FvH4_2g06181

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008837
Physical Location & Seq
Reverse (-)
5133632 .. 5135639
2008 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06181.t1

Sequence Viewer

Length: 846 bp
ATGATGGCTGACGAGTATATGAAGAACAGCTCTAACATGCTGGACTTCCTTTCTGAAATGGAAAAATTCCTCACACTTGCTCGCAATAGCCATTCATATATTACAACTGTGATTGAAAAGTACTTTAAGATCCACAATGGCATGAGAGGCAGAACTGAGTACTACAGGAAGATTGCGGATAACTTGAAGAAGCTCAGAGACTCTGATAATACCTTTGATCATTCTGCTGAAAACTTGCTCAAGAAGGCAGAAAGTTTGAAGATCCAGCATGAAGAAATGCTTCAGAAGTTTCAAGGTGAATTCATTAAGCTTTGTAAGAAGGAGAAACACTTGAAATCATGGGAGAACGTTGTGCTTATGATATTTGTAGGTGCTATGGTTGGATTGGCTATTGCCGGGATTGTGACTGCAGTTATAGCTGCTCCATCTGTTGCAACCGCAATTGCTGCCTGTACTTTACCGATAGCAATGGCTGGACACTGGTCTATCACTTGGTTTGAGGAGTCTGCAAAAGCCTTTAAGTATGAGCTGGAAGTAAATGAGGCGAGGCGCGAGGAAACTAGAGTTGCCATCAAAGAGTTGGGCAACATCAAGTCTTCGATTGATGGTGTTATTACACAGATTAATTCAGTCATCTACAATCCGAGCTCGGCAATGGATCGGGAACAAATAGATTTCACAATGAAGGCAATCAGGAGCAAACTGGGAGCGTTTCTGAATAAAGTTAAGGATTTGGAGAGAGACGTTGAAAAGGCCCGAAGGGAAATAAGGAAGGCAAGGGATGATGTTCATATCACGGGCACGCATGTAAAAAAAAGGAGCATTTTGTTCTCAAAACTAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

282

Amino Acids

32.08

Weight (kDa)

9.27

Isoelectric Point (pI)

33.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 4 - 262 9.2e-37 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 552
AciI CCGC 2 cut(s) 176, 438
AclI AACGTT 1 cut(s) 348
AclWI GGATC 3 cut(s) 124, 256, 666
AcsI RAATTY 2 cut(s) 65, 299
AcuI CTGAAG 1 cut(s) 266
AfaI GTAC 3 cut(s) 122, 161, 454
AgsI TTSAA 6 cut(s) 116, 187, 259, 293, 334, 749
AluBI AGCT 6 cut(s) 30, 193, 310, 419, 529, 648
AluI AGCT 6 cut(s) 30, 193, 310, 419, 529, 648
Alw21I GWGCWC 1 cut(s) 650
Alw26I GTCTC 2 cut(s) 192, 735
AlwI GGATC 3 cut(s) 124, 256, 666
AoxI GGCC 1 cut(s) 753
ApeKI GCWGC 2 cut(s) 419, 446
ApoI RAATTY 2 cut(s) 65, 299
AseI ATTAAT 1 cut(s) 624
Asp700I GAANNNNTTC 1 cut(s) 279
AspLEI GCGC 1 cut(s) 552
AspS9I GGNCC 1 cut(s) 754
AsuC2I CCSGG 1 cut(s) 397
AsuHPI GGTGA 1 cut(s) 308
BaeGI GKGCMC 1 cut(s) 803
BanII GRGCYC 1 cut(s) 650
BbsI GAAGAC 1 cut(s) 588
Bbv12I GWGCWC 1 cut(s) 650
BbvI GCAGC 2 cut(s) 406, 433
BccI CCATC 3 cut(s) 433, 578, 599
BclI TGATCA 1 cut(s) 217
BcnI CCSGG 1 cut(s) 397
BcoDI GTCTC 2 cut(s) 192, 735
BfaI CTAG 2 cut(s) 561, 839
BfmI CTRYAG 2 cut(s) 163, 408
BisI GCNGC 2 cut(s) 420, 447
BlsI GCNGC 2 cut(s) 421, 448
BmcAI AGTACT 2 cut(s) 122, 161
Bme1390I CCNGG 1 cut(s) 397
BmgT120I GGNCC 1 cut(s) 754
BmrFI CCNGG 1 cut(s) 397
BmrI ACTGGG 1 cut(s) 713
BmuI ACTGGG 1 cut(s) 713
BoxI GACNNNNGTC 1 cut(s) 481
BpiI GAAGAC 1 cut(s) 588
BpuEI CTTGAG 1 cut(s) 224
BpuMI CCSGG 1 cut(s) 397
Bse1I ACTGG 2 cut(s) 485, 708
Bse3DI GCAATG 2 cut(s) 474, 660
BseGI GGATG 1 cut(s) 787
BseMI GCAATG 2 cut(s) 474, 660
BseMII CTCAG 2 cut(s) 147, 208
BseNI ACTGG 2 cut(s) 485, 708
BseRI GAGGAG 1 cut(s) 515
BseSI GKGCMC 1 cut(s) 803
BseXI GCAGC 2 cut(s) 406, 433
Bsh1236I CGCG 1 cut(s) 552
BshFI GGCC 1 cut(s) 755
BsiHKAI GWGCWC 1 cut(s) 650
BsiSI CCGG 1 cut(s) 396
BsmAI GTCTC 2 cut(s) 192, 735
BsmBI CGTCTC 1 cut(s) 735
BsnI GGCC 1 cut(s) 755
Bsp1286I GDGCHC 2 cut(s) 650, 803
Bsp143I GATC 4 cut(s) 129, 217, 261, 658
BspACI CCGC 2 cut(s) 176, 438
BspANI GGCC 1 cut(s) 755
BspCNI CTCAG 2 cut(s) 148, 207
BspFNI CGCG 1 cut(s) 552
BspMAI CTGCAG 1 cut(s) 412
BspPI GGATC 3 cut(s) 124, 256, 666
BsrDI GCAATG 2 cut(s) 474, 660
BsrI ACTGG 2 cut(s) 485, 708
BssMI GATC 4 cut(s) 129, 217, 261, 658
Bst4CI ACNGT 1 cut(s) 109
BstAPI GCANNNNNTGC 1 cut(s) 446
BstC8I GCNNGC 2 cut(s) 82, 803
BstDEI CTNAG 2 cut(s) 156, 194
BstF5I GGATG 1 cut(s) 787
BstFNI CGCG 1 cut(s) 552
BstHHI GCGC 1 cut(s) 552
BstKTI GATC 4 cut(s) 132, 220, 264, 661
BstMAI GTCTC 2 cut(s) 192, 735
BstMBI GATC 4 cut(s) 129, 217, 261, 658
BstMWI GCNNNNNNNGC 3 cut(s) 147, 416, 446
BstNSI RCATGY 2 cut(s) 40, 809
BstPAI GACNNNNGTC 1 cut(s) 481
BstSCI CCNGG 1 cut(s) 395
BstSFI CTRYAG 2 cut(s) 163, 408
BstSLI GKGCMC 1 cut(s) 803
BstUI CGCG 1 cut(s) 552
BstV1I GCAGC 2 cut(s) 406, 433
BstV2I GAAGAC 1 cut(s) 588
BstX2I RGATCY 2 cut(s) 129, 261
BstYI RGATCY 2 cut(s) 129, 261
BsuRI GGCC 1 cut(s) 755
BtsCI GGATG 1 cut(s) 787
BtsIMutI CAGTG 1 cut(s) 478
Cac8I GCNNGC 2 cut(s) 82, 803
CfoI GCGC 1 cut(s) 552
Cfr13I GGNCC 1 cut(s) 754
Csp6I GTAC 3 cut(s) 121, 160, 453
CviAII CATG 6 cut(s) 37, 142, 269, 339, 806, 843
CviQI GTAC 3 cut(s) 121, 160, 453
DdeI CTNAG 2 cut(s) 156, 194
DpnI GATC 4 cut(s) 131, 219, 263, 660
DpnII GATC 4 cut(s) 129, 217, 261, 658
Ecl136II GAGCTC 1 cut(s) 648
Eco24I GRGCYC 1 cut(s) 650
Eco53kI GAGCTC 1 cut(s) 648
Eco57I CTGAAG 1 cut(s) 266
EcoICRI GAGCTC 1 cut(s) 648
EcoRI GAATTC 1 cut(s) 299
EcoT38I GRGCYC 1 cut(s) 650
Esp3I CGTCTC 1 cut(s) 735
FaeI CATG 6 cut(s) 40, 145, 272, 342, 809, 846
FalI AAGNNNNNCTT 2 cut(s) 264, 296
FatI CATG 6 cut(s) 36, 141, 268, 338, 805, 842
FbaI TGATCA 1 cut(s) 217
Fnu4HI GCNGC 2 cut(s) 420, 447
FokI GGATG 1 cut(s) 794
FriOI GRGCYC 1 cut(s) 650
Fsp4HI GCNGC 2 cut(s) 420, 447
FspBI CTAG 2 cut(s) 561, 839
GlaI GCGC 1 cut(s) 551
GluI GCNGC 2 cut(s) 420, 447
HaeIII GGCC 1 cut(s) 755
HapII CCGG 1 cut(s) 396
HhaI GCGC 1 cut(s) 552
Hin1II CATG 6 cut(s) 40, 145, 272, 342, 809, 846
Hin6I GCGC 1 cut(s) 550
HinP1I GCGC 1 cut(s) 550
HindIII AAGCTT 1 cut(s) 308
HinfI GANTC 2 cut(s) 200, 503
HpaII CCGG 1 cut(s) 396
HphI GGTGA 1 cut(s) 308
Hpy188I TCNGA 6 cut(s) 55, 197, 205, 285, 645, 717
Hpy188III TCNNGA 3 cut(s) 241, 662, 694
HpyAV CCTTC 5 cut(s) 238, 313, 679, 753, 766
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4IV ACGT 2 cut(s) 348, 744
HpyCH4V TGCA 3 cut(s) 410, 434, 509
HpyF10VI GCNNNNNNNGC 3 cut(s) 147, 416, 446
HpyF3I CTNAG 2 cut(s) 156, 194
HpySE526I ACGT 2 cut(s) 348, 744
Hsp92II CATG 6 cut(s) 40, 145, 272, 342, 809, 846
HspAI GCGC 1 cut(s) 550
Ksp22I TGATCA 1 cut(s) 217
Kzo9I GATC 4 cut(s) 129, 217, 261, 658
LmnI GCTCC 4 cut(s) 427, 696, 707, 819
Lsp1109I GCAGC 2 cut(s) 406, 433
MaeI CTAG 2 cut(s) 561, 839
MaeII ACGT 2 cut(s) 348, 744
MaeIII GTNAC 1 cut(s) 403
MalI GATC 4 cut(s) 131, 219, 263, 660
MboI GATC 4 cut(s) 129, 217, 261, 658
MboII GAAGA 6 cut(s) 34, 181, 199, 271, 284, 588
MfeI CAATTG 1 cut(s) 441
MflI RGATCY 2 cut(s) 129, 261
MhlI GDGCHC 2 cut(s) 650, 803
MluCI AATT 4 cut(s) 65, 299, 441, 625
MlyI GAGTC 2 cut(s) 194, 512
MmeI TCCRAC 1 cut(s) 361
MnlI CCTC 6 cut(s) 80, 140, 493, 535, 540, 547
MroXI GAANNNNTTC 1 cut(s) 279
MseI TTAA 5 cut(s) 126, 306, 519, 624, 726
MspI CCGG 1 cut(s) 396
MspR9I CCNGG 1 cut(s) 397
MunI CAATTG 1 cut(s) 441
MvnI CGCG 1 cut(s) 552
MwoI GCNNNNNNNGC 3 cut(s) 147, 416, 446
NciI CCSGG 1 cut(s) 397
NdeII GATC 4 cut(s) 129, 217, 261, 658
NlaIII CATG 6 cut(s) 40, 145, 272, 342, 809, 846
NmeAIII GCCGAG 1 cut(s) 629
NmuCI GTSAC 1 cut(s) 403
NspI RCATGY 2 cut(s) 40, 809
PdmI GAANNNNTTC 1 cut(s) 279
PkrI GCNGC 2 cut(s) 421, 448
PleI GAGTC 2 cut(s) 194, 511
PpsI GAGTC 2 cut(s) 194, 511
PshAI GACNNNNGTC 1 cut(s) 481
PshBI ATTAAT 1 cut(s) 624
Psp124BI GAGCTC 1 cut(s) 650
Psp1406I AACGTT 1 cut(s) 348
PspPI GGNCC 1 cut(s) 754
PstI CTGCAG 1 cut(s) 412
PsuI RGATCY 2 cut(s) 129, 261
RsaI GTAC 3 cut(s) 122, 161, 454
RsaNI GTAC 3 cut(s) 121, 160, 453
SacI GAGCTC 1 cut(s) 650
SaqAI TTAA 5 cut(s) 126, 306, 519, 624, 726
SatI GCNGC 2 cut(s) 420, 447
Sau3AI GATC 4 cut(s) 129, 217, 261, 658
Sau96I GGNCC 1 cut(s) 754
ScaI AGTACT 2 cut(s) 122, 161
SchI GAGTC 2 cut(s) 194, 512
ScrFI CCNGG 1 cut(s) 397
SduI GDGCHC 2 cut(s) 650, 803
SfcI CTRYAG 2 cut(s) 163, 408
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 4 cut(s) 65, 299, 441, 625
SsiI CCGC 2 cut(s) 176, 438
SspMI CTAG 2 cut(s) 561, 839
SstI GAGCTC 1 cut(s) 650
StyD4I CCNGG 1 cut(s) 395
TaaI ACNGT 1 cut(s) 109
TaiI ACGT 2 cut(s) 351, 747
TaqI TCGA 1 cut(s) 599
TasI AATT 4 cut(s) 65, 299, 441, 625
TatI WGTACW 3 cut(s) 120, 159, 452
Tru1I TTAA 5 cut(s) 126, 306, 519, 624, 726
Tru9I TTAA 5 cut(s) 126, 306, 519, 624, 726
TscAI CASTG 1 cut(s) 485
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 2 cut(s) 419, 446
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 6 cut(s) 35, 84, 285, 292, 698, 779
TspRI CASTG 1 cut(s) 485
VspI ATTAAT 1 cut(s) 624
XapI RAATTY 2 cut(s) 65, 299
XceI RCATGY 2 cut(s) 40, 809
XcmI CCANNNNNNNNNTGG 1 cut(s) 577
XmnI GAANNNNTTC 1 cut(s) 279
XspI CTAG 2 cut(s) 561, 839
ZrmI AGTACT 2 cut(s) 122, 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.