Rh6AG120900

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0008837
Physical Location & Seq
Forward (+)
18088447 .. 18093033
4587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG120900.1

Sequence Viewer

Length: 1176 bp
ATGACGACATATACAGCTGAGATTTCTATGAAATATTTGTATAGAAAGTTAGTCTCCACAGCCGAATGGAAGTCATTTCTGGAAGTTTGCAAGAAAAATGCAGACTTGCAATCGTGGGACACAGACCTCAAAACCCATGCAGAAGAAATCTTGAAAACCCTCAAGAGTGAAGGAGGACTCTCGATTGATTCCTGTAAACGAATCACACACTATGCGTGGGATTCAAACAAGAAGATTGTGCATGCAATCTTGGCACTCAAGGAAGGTATAACTAAAAGGAAGGGGAGTAGTAACAAGAAGAAGAGAAATGAAGAAAGGGTGAACATCCTGGCTGACGAATATATTAAGAATAGCTCAAACATGCTGGATTTCCTTTCTGAGATAGAAAACTTCCTCATGCTTGCTCGTAATAGCCATTCATATATTGCAACTGTGGTTCAACAGTACTTTGAGATACACAATGGTATGAGTGGAGCTGAGTCCTACGGGAAGATTGCGGATAACTTAAAAAAGCTCAAGGACTCTGATAATACCTTTGACCATTCTGCTGAAAACTTGCTCAAAAAGGCAGTAAGTTTGAAGAGTCAGCATGAAGAAATGCTTCATAATTTGCAAGGCGAAATCATTAAACTTAGTAAGAAGGAGAAACGCGCGAAATCATTGAAGAACGTCGCGTATATGATATTTGTAGGCGCTGTGGTTGGTTTGGCTATTGCCGGAATTGTGACTGCAGTTATAGCTGCTCCACCTGTTGCAGGAGCAATTACTGCTTGCATTGTCCCCATAGCAATGGCTGGAAACTGGACTATCTATTGGTTTGAAGAGTCTGAAAAAGCATTTAAGAATCAGCGGGAAGTAAATGAGGCGAGGCGTAAGGAAACTAGAATTGCCATTAAAGAATTGGGTAACATTAAGTCTTCGATTGATGGTGTTATTAAACAGATTAATTCAGTCATGTACAATCCAAGCTCTGAAATGGATCGGGAACAATTGGATTTCAGAATGAAGGAAATCAAGAGCAAGCTGGGAGCGTTTCTGAATAAGGTTGAGGATTTGGAGAAGGACTTAGAAAAGACCAAAAAGGATATAAGGAAGGCAAGGGATGATGTTCATATCATGGACACCCAGGTCACGCAGGTAAAAAAAAAGAGCATTTTGTTTTCAAAACTAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

391

Amino Acids

44.36

Weight (kDa)

9.29

Isoelectric Point (pI)

37.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 36 - 369 6.2e-43 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1127
Acc36I ACCTGC 1 cut(s) 1126
AccII CGCG 3 cut(s) 651, 653, 674
AciI CCGC 2 cut(s) 497, 850
AclWI GGATC 1 cut(s) 987
AfaI GTAC 2 cut(s) 446, 959
AfiI CCNNNNNNNGG 1 cut(s) 755
AgsI TTSAA 7 cut(s) 154, 225, 440, 580, 664, 821, 1164
AjnI CCWGG 2 cut(s) 327, 1125
AluBI AGCT 7 cut(s) 17, 354, 476, 514, 740, 969, 1024
AluI AGCT 7 cut(s) 17, 354, 476, 514, 740, 969, 1024
Alw26I GTCTC 1 cut(s) 58
AlwI GGATC 1 cut(s) 987
ApeKI GCWGC 1 cut(s) 740
AseI ATTAAT 1 cut(s) 945
Asp700I GAANNNNTTC 1 cut(s) 600
AspLEI GCGC 2 cut(s) 653, 695
AsuHPI GGTGA 1 cut(s) 331
BbsI GAAGAC 1 cut(s) 909
BbvI GCAGC 1 cut(s) 727
BccI CCATC 1 cut(s) 920
BciT130I CCWGG 2 cut(s) 329, 1127
BcoDI GTCTC 1 cut(s) 58
BfaI CTAG 2 cut(s) 882, 1169
BfmI CTRYAG 1 cut(s) 729
BfoI RGCGCY 1 cut(s) 696
BfuAI ACCTGC 1 cut(s) 1126
BisI GCNGC 1 cut(s) 741
BlsI GCNGC 1 cut(s) 742
BmcAI AGTACT 1 cut(s) 446
Bme1390I CCNGG 2 cut(s) 329, 1127
BmrFI CCNGG 2 cut(s) 329, 1127
BpiI GAAGAC 1 cut(s) 909
BpuEI CTTGAG 3 cut(s) 146, 242, 500
BsaJI CCNNGG 1 cut(s) 1125
Bsc4I CCNNNNNNNGG 1 cut(s) 755
Bse1I ACTGG 1 cut(s) 806
Bse3DI GCAATG 1 cut(s) 795
BseBI CCWGG 2 cut(s) 329, 1127
BseDI CCNNGG 1 cut(s) 1125
BseGI GGATG 2 cut(s) 324, 1108
BseLI CCNNNNNNNGG 1 cut(s) 755
BseMI GCAATG 1 cut(s) 795
BseMII CTCAG 3 cut(s) 9, 369, 468
BseNI ACTGG 1 cut(s) 806
BseXI GCAGC 1 cut(s) 727
BseYI CCCAGC 1 cut(s) 1024
Bsh1236I CGCG 3 cut(s) 651, 653, 674
BsiSI CCGG 1 cut(s) 717
BslFI GGGAC 2 cut(s) 131, 764
BslI CCNNNNNNNGG 1 cut(s) 755
BsmAI GTCTC 1 cut(s) 58
BsmFI GGGAC 2 cut(s) 131, 764
Bsp1407I TGTACA 1 cut(s) 957
Bsp143I GATC 1 cut(s) 979
BspACI CCGC 2 cut(s) 497, 850
BspCNI CTCAG 3 cut(s) 10, 370, 469
BspFNI CGCG 3 cut(s) 651, 653, 674
BspMAI CTGCAG 1 cut(s) 733
BspMI ACCTGC 1 cut(s) 1126
BspPI GGATC 1 cut(s) 987
BsrDI GCAATG 1 cut(s) 795
BsrGI TGTACA 1 cut(s) 957
BsrI ACTGG 1 cut(s) 806
BssECI CCNNGG 1 cut(s) 1125
BssMI GATC 1 cut(s) 979
Bst2UI CCWGG 2 cut(s) 329, 1127
Bst4CI ACNGT 2 cut(s) 433, 444
Bst6I CTCTTC 3 cut(s) 296, 575, 816
BstAPI GCANNNNNTGC 1 cut(s) 767
BstAUI TGTACA 1 cut(s) 957
BstC8I GCNNGC 4 cut(s) 243, 402, 772, 1022
BstDEI CTNAG 5 cut(s) 18, 378, 477, 632, 1066
BstENI CCTNNNNNAGG 1 cut(s) 753
BstF5I GGATG 2 cut(s) 324, 1108
BstFNI CGCG 3 cut(s) 651, 653, 674
BstH2I RGCGCY 1 cut(s) 696
BstHHI GCGC 2 cut(s) 653, 695
BstKTI GATC 1 cut(s) 982
BstMAI GTCTC 1 cut(s) 58
BstMBI GATC 1 cut(s) 979
BstMWI GCNNNNNNNGC 3 cut(s) 251, 737, 767
BstNI CCWGG 2 cut(s) 329, 1127
BstNSI RCATGY 2 cut(s) 245, 364
BstSCI CCNGG 2 cut(s) 327, 1125
BstSFI CTRYAG 1 cut(s) 729
BstUI CGCG 3 cut(s) 651, 653, 674
BstV1I GCAGC 1 cut(s) 727
BstV2I GAAGAC 1 cut(s) 909
BstXI CCANNNNNNTGG 1 cut(s) 790
BtsCI GGATG 2 cut(s) 324, 1108
BveI ACCTGC 1 cut(s) 1126
Cac8I GCNNGC 4 cut(s) 243, 402, 772, 1022
CfoI GCGC 2 cut(s) 653, 695
Csp6I GTAC 2 cut(s) 445, 958
CviAII CATG 8 cut(s) 137, 242, 361, 397, 590, 955, 1117, 1173
CviQI GTAC 2 cut(s) 445, 958
DdeI CTNAG 5 cut(s) 18, 378, 477, 632, 1066
DpnI GATC 1 cut(s) 981
DpnII GATC 1 cut(s) 979
DrdI GACNNNNNNGTC 1 cut(s) 1127
DseDI GACNNNNNNGTC 1 cut(s) 1127
Eam1104I CTCTTC 3 cut(s) 296, 575, 816
EarI CTCTTC 3 cut(s) 296, 575, 816
EcoNI CCTNNNNNAGG 1 cut(s) 753
EcoRII CCWGG 2 cut(s) 327, 1125
FaeI CATG 8 cut(s) 140, 245, 364, 400, 593, 958, 1120, 1176
FalI AAGNNNNNCTT 2 cut(s) 585, 617
FaqI GGGAC 2 cut(s) 131, 764
FatI CATG 8 cut(s) 136, 241, 360, 396, 589, 954, 1116, 1172
FauI CCCGC 1 cut(s) 843
Fnu4HI GCNGC 1 cut(s) 741
FokI GGATG 2 cut(s) 311, 1115
Fsp4HI GCNGC 1 cut(s) 741
FspBI CTAG 2 cut(s) 882, 1169
GlaI GCGC 2 cut(s) 652, 694
GluI GCNGC 1 cut(s) 741
GsaI CCCAGC 1 cut(s) 1028
HaeII RGCGCY 1 cut(s) 696
HapII CCGG 1 cut(s) 717
HhaI GCGC 2 cut(s) 653, 695
Hin1II CATG 8 cut(s) 140, 245, 364, 400, 593, 958, 1120, 1176
Hin6I GCGC 2 cut(s) 651, 693
HinP1I GCGC 2 cut(s) 651, 693
HinfI GANTC 9 cut(s) 177, 188, 201, 221, 479, 521, 583, 824, 844
HpaII CCGG 1 cut(s) 717
HphI GGTGA 1 cut(s) 331
Hpy166II GTNNAC 2 cut(s) 197, 322
Hpy188I TCNGA 6 cut(s) 379, 526, 829, 973, 1001, 1038
Hpy188III TCNNGA 6 cut(s) 80, 151, 163, 181, 983, 1015
Hpy8I GTNNAC 2 cut(s) 197, 322
Hpy99I CGWCG 1 cut(s) 674
HpyAV CCTTC 7 cut(s) 164, 257, 274, 634, 1000, 1054, 1087
HpyCH4III ACNGT 2 cut(s) 433, 444
HpyCH4IV ACGT 1 cut(s) 669
HpyF10VI GCNNNNNNNGC 3 cut(s) 251, 737, 767
HpyF3I CTNAG 5 cut(s) 18, 378, 477, 632, 1066
HpySE526I ACGT 1 cut(s) 669
Hsp92II CATG 8 cut(s) 140, 245, 364, 400, 593, 958, 1120, 1176
HspAI GCGC 2 cut(s) 651, 693
Kzo9I GATC 1 cut(s) 979
LmnI GCTCC 4 cut(s) 473, 748, 758, 1028
Lsp1109I GCAGC 1 cut(s) 727
MaeI CTAG 2 cut(s) 882, 1169
MaeII ACGT 1 cut(s) 669
MaeIII GTNAC 4 cut(s) 290, 724, 905, 1129
MalI GATC 1 cut(s) 981
MboI GATC 1 cut(s) 979
MfeI CAATTG 1 cut(s) 989
MluCI AATT 7 cut(s) 607, 720, 762, 885, 899, 946, 989
MlyI GAGTC 5 cut(s) 171, 488, 515, 592, 833
MnlI CCTC 7 cut(s) 137, 167, 170, 404, 856, 861, 1042
MroXI GAANNNNTTC 1 cut(s) 600
MseI TTAA 8 cut(s) 345, 506, 627, 840, 894, 912, 936, 945
MslI CAYNNNNRTG 1 cut(s) 788
MspA1I CMGCKG 2 cut(s) 17, 850
MspI CCGG 1 cut(s) 717
MspR9I CCNGG 2 cut(s) 329, 1127
MunI CAATTG 1 cut(s) 989
MvaI CCWGG 2 cut(s) 329, 1127
MvnI CGCG 3 cut(s) 651, 653, 674
MwoI GCNNNNNNNGC 3 cut(s) 251, 737, 767
NdeII GATC 1 cut(s) 979
NlaIII CATG 8 cut(s) 140, 245, 364, 400, 593, 958, 1120, 1176
NmuCI GTSAC 2 cut(s) 724, 1129
NspI RCATGY 2 cut(s) 245, 364
PaeI GCATGC 1 cut(s) 245
PdmI GAANNNNTTC 1 cut(s) 600
PfeI GAWTC 4 cut(s) 188, 201, 221, 844
PkrI GCNGC 1 cut(s) 742
PleI GAGTC 5 cut(s) 171, 487, 515, 591, 832
PpsI GAGTC 5 cut(s) 171, 487, 515, 591, 832
PshBI ATTAAT 1 cut(s) 945
Psp6I CCWGG 2 cut(s) 327, 1125
PspFI CCCAGC 1 cut(s) 1024
PspGI CCWGG 2 cut(s) 327, 1125
PstI CTGCAG 1 cut(s) 733
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 2 cut(s) 446, 959
RsaNI GTAC 2 cut(s) 445, 958
RseI CAYNNNNRTG 1 cut(s) 788
SaqAI TTAA 8 cut(s) 345, 506, 627, 840, 894, 912, 936, 945
SatI GCNGC 1 cut(s) 741
Sau3AI GATC 1 cut(s) 979
ScaI AGTACT 1 cut(s) 446
SchI GAGTC 5 cut(s) 171, 488, 515, 592, 833
ScrFI CCNGG 2 cut(s) 329, 1127
SfcI CTRYAG 1 cut(s) 729
SmiMI CAYNNNNRTG 1 cut(s) 788
SmlI CTYRAG 3 cut(s) 161, 257, 515
SmoI CTYRAG 3 cut(s) 161, 257, 515
SphI GCATGC 1 cut(s) 245
Sse9I AATT 7 cut(s) 607, 720, 762, 885, 899, 946, 989
SsiI CCGC 2 cut(s) 497, 850
SspI AATATT 1 cut(s) 35
SspMI CTAG 2 cut(s) 882, 1169
StyD4I CCNGG 2 cut(s) 327, 1125
TaaI ACNGT 2 cut(s) 433, 444
TaiI ACGT 1 cut(s) 672
TaqI TCGA 2 cut(s) 182, 920
TasI AATT 7 cut(s) 607, 720, 762, 885, 899, 946, 989
TatI WGTACW 2 cut(s) 444, 957
TfiI GAWTC 4 cut(s) 188, 201, 221, 844
Tru1I TTAA 8 cut(s) 345, 506, 627, 840, 894, 912, 936, 945
Tru9I TTAA 8 cut(s) 345, 506, 627, 840, 894, 912, 936, 945
TseFI GTSAC 2 cut(s) 724, 1129
TseI GCWGC 1 cut(s) 740
Tsp45I GTSAC 2 cut(s) 724, 1129
TspDTI ATGAA 7 cut(s) 44, 324, 408, 593, 606, 1019, 1100
VspI ATTAAT 1 cut(s) 945
XagI CCTNNNNNAGG 1 cut(s) 753
XceI RCATGY 2 cut(s) 245, 364
XcmI CCANNNNNNNNNTGG 1 cut(s) 898
XmnI GAANNNNTTC 1 cut(s) 600
XspI CTAG 2 cut(s) 882, 1169
ZrmI AGTACT 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.