Rroxscaffold_7G00204950

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008837
Physical Location & Seq
Reverse (-)
54432853 .. 54434253
1401 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00204950.1

Sequence Viewer

Length: 1038 bp
ATGTTTGACCCCGACACCATTCCCACAGCTGAATGGAAGTCATTTCAGGAAGTTTGCGAGAAAAATGCGGACATGCAATCGTGTGACACAGACCTCAAAACCCATGCAAAAGAGATCTTGAAAACCTTCAAGGGTGAAGGAGGTCTCTCGATTGATTCTTGGAAACCATTAACACTCTATGCGTGGTATTCAAACAAGAAGATTGTGCAAGAAATCTTGGAATTCAAGGAAGATATAACTAAAGGGAAGGGGATTAGTAAGAAGAAGAAGACAAATGAAGAAAGGGTGAACATCCCGGCGCCGAGGTTGGTTTGGCTATTGCGGAATTGTGATTGCAGTTATAGGTGCTCCACCCGTTGCGAAGCAATTGCTGCTGGCATTGTCCCGATAGCAATGGGTGGACACCGGACTATCTCTTGGTTTGAAGAGTCTGAAAAAGCATTTAAGAATCAGCGGGAAGTAAATGAGGCGGGGCGTAAGGAAACTAGAATTGCCATTAAAGAGTTGGGCAACATTAAGACTTTGATTGATGGTGTTATTAAACAGATTAATTCAGTCATGTACAATCCAGGCTCTCAAATGGATTGGGAACAATTAGATTTCACAATGAAGGAAATCAAGAGCAAGCTAGGAGCGTTTCTGAATAAGATTGAGGATTTGGAGAAGGAAGTAGAAAAGACCAAAAAAGATATATGCAAGGCAAGGGATGATGTTCATATCATGGACACCTGGTCACACAGTCCAAAAAAGGAGCATTTTGTTTTTAAAACTAGCATGAACTTGTTATTAGCTCCCATGGCCATCTGCTTGTTGATCCGTAGTCTTGAAGGCTTAGGCTATGTTTGGTATGGCTGTACTTCTCGAAAAGCCGACTTCGCTTATTTCGAGAATAAGTGGCCGGAAAGCAAAGCGGCCGAGTGTTTGGTAAACGGCTTTTTTATAAGTGTCGTCGTTGACAAAAGCAGTGTCAAAGTGTTTGGTAAACTACTACACCAATTTTTTCAATCGACGACAGTTTTCATCGTCGTCCGATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

345

Amino Acids

39.53

Weight (kDa)

8.64

Isoelectric Point (pI)

32.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 124 - 237 5.2e-11 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 941
AccB1I GGYRCC 1 cut(s) 298
AciI CCGC 5 cut(s) 68, 322, 454, 470, 911
AclWI GGATC 1 cut(s) 808
AcoI YGGCCR 3 cut(s) 798, 896, 912
AcsI RAATTY 1 cut(s) 221
AcyI GRCGYC 1 cut(s) 299
AfaI GTAC 2 cut(s) 563, 856
AgsI TTSAA 7 cut(s) 121, 130, 192, 226, 425, 827, 1004
AhdI GACNNNNNGTC 1 cut(s) 730
AjnI CCWGG 2 cut(s) 568, 728
AluBI AGCT 3 cut(s) 29, 628, 791
AluI AGCT 3 cut(s) 29, 628, 791
Alw21I GWGCWC 1 cut(s) 350
Alw26I GTCTC 1 cut(s) 149
AlwI GGATC 1 cut(s) 808
AoxI GGCC 3 cut(s) 798, 896, 912
ApeKI GCWGC 1 cut(s) 371
ApoI RAATTY 1 cut(s) 221
ArsI GACNNNNNNTTYG 2 cut(s) 951, 983
AseI ATTAAT 1 cut(s) 549
Asp700I GAANNNNTTC 1 cut(s) 125
AspLEI GCGC 1 cut(s) 301
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 2 cut(s) 146, 298
BalI TGGCCA 1 cut(s) 800
BanI GGYRCC 1 cut(s) 298
BbsI GAAGAC 1 cut(s) 275
Bbv12I GWGCWC 1 cut(s) 350
BbvI GCAGC 1 cut(s) 358
BccI CCATC 2 cut(s) 524, 809
BceAI ACGGC 1 cut(s) 946
BcgI CGANNNNNNTGC 4 cut(s) 47, 81, 350, 384
BciT130I CCWGG 2 cut(s) 570, 730
BcnI CCSGG 1 cut(s) 296
BcoDI GTCTC 1 cut(s) 149
BfaI CTAG 3 cut(s) 486, 629, 771
BfoI RGCGCY 1 cut(s) 302
BglII AGATCT 1 cut(s) 114
BisI GCNGC 2 cut(s) 372, 912
BlsI GCNGC 2 cut(s) 373, 913
Bme1390I CCNGG 3 cut(s) 296, 570, 730
BmeRI GACNNNNNGTC 1 cut(s) 730
BmiI GGNNCC 1 cut(s) 300
BmrFI CCNGG 3 cut(s) 296, 570, 730
BpiI GAAGAC 1 cut(s) 275
Bpu10I CCTNAGC 1 cut(s) 832
BpuMI CCSGG 1 cut(s) 296
BsaHI GRCGYC 1 cut(s) 299
BsaI GGTCTC 1 cut(s) 149
BsaJI CCNNGG 2 cut(s) 302, 795
BsaWI WCCGGW 1 cut(s) 405
Bse3DI GCAATG 1 cut(s) 399
BseBI CCWGG 2 cut(s) 570, 730
BseDI CCNNGG 2 cut(s) 302, 795
BseGI GGATG 2 cut(s) 291, 712
BseMI GCAATG 1 cut(s) 399
BseX3I CGGCCG 1 cut(s) 912
BseXI GCAGC 1 cut(s) 358
Bsh1285I CGRYCG 1 cut(s) 915
BshFI GGCC 3 cut(s) 800, 898, 914
BshNI GGYRCC 1 cut(s) 298
BsiEI CGRYCG 1 cut(s) 915
BsiHKAI GWGCWC 1 cut(s) 350
BsiSI CCGG 3 cut(s) 296, 406, 899
BslFI GGGAC 1 cut(s) 368
BsmAI GTCTC 1 cut(s) 149
BsmFI GGGAC 1 cut(s) 368
BsnI GGCC 3 cut(s) 800, 898, 914
Bso31I GGTCTC 1 cut(s) 149
Bsp1286I GDGCHC 1 cut(s) 350
Bsp1407I TGTACA 1 cut(s) 561
Bsp143I GATC 3 cut(s) 114, 813, 1031
Bsp19I CCATGG 1 cut(s) 795
BspACI CCGC 5 cut(s) 68, 322, 454, 470, 911
BspANI GGCC 3 cut(s) 800, 898, 914
BspLI GGNNCC 1 cut(s) 300
BspPI GGATC 1 cut(s) 808
BspT107I GGYRCC 1 cut(s) 298
BspTNI GGTCTC 1 cut(s) 149
BsrDI GCAATG 1 cut(s) 399
BsrGI TGTACA 1 cut(s) 561
BssECI CCNNGG 2 cut(s) 302, 795
BssMI GATC 3 cut(s) 114, 813, 1031
BssNI GRCGYC 1 cut(s) 299
BssT1I CCWWGG 1 cut(s) 795
Bst2UI CCWGG 2 cut(s) 570, 730
Bst4CI ACNGT 2 cut(s) 740, 1015
Bst6I CTCTTC 1 cut(s) 420
BstACI GRCGYC 1 cut(s) 299
BstAPI GCANNNNNTGC 1 cut(s) 371
BstAUI TGTACA 1 cut(s) 561
BstC8I GCNNGC 2 cut(s) 376, 626
BstDEI CTNAG 1 cut(s) 832
BstDSI CCRYGG 1 cut(s) 795
BstF5I GGATG 2 cut(s) 291, 712
BstH2I RGCGCY 1 cut(s) 302
BstHHI GCGC 1 cut(s) 301
BstKTI GATC 3 cut(s) 117, 816, 1034
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 3 cut(s) 114, 813, 1031
BstMCI CGRYCG 1 cut(s) 915
BstMWI GCNNNNNNNGC 3 cut(s) 371, 797, 875
BstNI CCWGG 2 cut(s) 570, 730
BstNSI RCATGY 1 cut(s) 76
BstSCI CCNGG 3 cut(s) 294, 568, 728
BstV1I GCAGC 1 cut(s) 358
BstV2I GAAGAC 1 cut(s) 275
BstX2I RGATCY 1 cut(s) 114
BstYI RGATCY 1 cut(s) 114
BstZI CGGCCG 1 cut(s) 912
BsuRI GGCC 3 cut(s) 800, 898, 914
BtgI CCRYGG 1 cut(s) 795
BtsCI GGATG 2 cut(s) 291, 712
BtsI GCAGTG 1 cut(s) 970
BtsIMutI CAGTG 1 cut(s) 970
Cac8I GCNNGC 2 cut(s) 376, 626
CfoI GCGC 1 cut(s) 301
CsiI ACCWGGT 1 cut(s) 728
Csp6I GTAC 2 cut(s) 562, 855
CviAII CATG 6 cut(s) 73, 104, 559, 721, 775, 796
CviQI GTAC 2 cut(s) 562, 855
DdeI CTNAG 1 cut(s) 832
DinI GGCGCC 1 cut(s) 300
DpnI GATC 3 cut(s) 116, 815, 1033
DpnII GATC 3 cut(s) 114, 813, 1031
DraI TTTAAA 1 cut(s) 766
DriI GACNNNNNGTC 1 cut(s) 730
EaeI YGGCCR 3 cut(s) 798, 896, 912
EagI CGGCCG 1 cut(s) 912
Eam1104I CTCTTC 1 cut(s) 420
Eam1105I GACNNNNNGTC 1 cut(s) 730
EarI CTCTTC 1 cut(s) 420
EclXI CGGCCG 1 cut(s) 912
Eco130I CCWWGG 1 cut(s) 795
Eco31I GGTCTC 1 cut(s) 149
Eco52I CGGCCG 1 cut(s) 912
EcoRI GAATTC 1 cut(s) 221
EcoRII CCWGG 2 cut(s) 568, 728
EcoT14I CCWWGG 1 cut(s) 795
EgeI GGCGCC 1 cut(s) 300
EheI GGCGCC 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 795
FaeI CATG 6 cut(s) 76, 107, 562, 724, 778, 799
FaqI GGGAC 1 cut(s) 368
FatI CATG 6 cut(s) 72, 103, 558, 720, 774, 795
FauI CCCGC 2 cut(s) 447, 463
Fnu4HI GCNGC 2 cut(s) 372, 912
FokI GGATG 2 cut(s) 278, 719
Fsp4HI GCNGC 2 cut(s) 372, 912
FspBI CTAG 3 cut(s) 486, 629, 771
GlaI GCGC 1 cut(s) 300
GluI GCNGC 2 cut(s) 372, 912
HaeII RGCGCY 1 cut(s) 302
HaeIII GGCC 3 cut(s) 800, 898, 914
HapII CCGG 3 cut(s) 296, 406, 899
HhaI GCGC 1 cut(s) 301
Hin1I GRCGYC 1 cut(s) 299
Hin1II CATG 6 cut(s) 76, 107, 562, 724, 778, 799
Hin6I GCGC 1 cut(s) 299
HinP1I GCGC 1 cut(s) 299
HincII GTYRAC 1 cut(s) 955
HindII GTYRAC 1 cut(s) 955
HinfI GANTC 3 cut(s) 155, 428, 448
HpaII CCGG 3 cut(s) 296, 406, 899
HphI GGTGA 2 cut(s) 146, 298
Hpy166II GTNNAC 5 cut(s) 289, 401, 928, 955, 983
Hpy188I TCNGA 3 cut(s) 433, 642, 1031
Hpy188III TCNNGA 8 cut(s) 47, 118, 148, 385, 619, 824, 861, 886
Hpy8I GTNNAC 5 cut(s) 289, 401, 928, 955, 983
Hpy99I CGWCG 3 cut(s) 953, 1012, 1028
HpyAV CCTTC 6 cut(s) 131, 136, 241, 604, 658, 821
HpyCH4III ACNGT 2 cut(s) 740, 1015
HpyCH4V TGCA 5 cut(s) 76, 107, 208, 336, 696
HpyF10VI GCNNNNNNNGC 3 cut(s) 371, 797, 875
HpyF3I CTNAG 1 cut(s) 832
Hsp92I GRCGYC 1 cut(s) 299
Hsp92II CATG 6 cut(s) 76, 107, 562, 724, 778, 799
HspAI GCGC 1 cut(s) 299
KasI GGCGCC 1 cut(s) 298
Kzo9I GATC 3 cut(s) 114, 813, 1031
LmnI GCTCC 4 cut(s) 353, 632, 751, 796
LpnPI CCDG 9 cut(s) 32, 309, 360, 419, 555, 582, 715, 742, 912
Lsp1109I GCAGC 1 cut(s) 358
MabI ACCWGGT 1 cut(s) 728
MaeI CTAG 3 cut(s) 486, 629, 771
MaeIII GTNAC 2 cut(s) 83, 732
MalI GATC 3 cut(s) 116, 815, 1033
MboI GATC 3 cut(s) 114, 813, 1031
MboII GAAGA 7 cut(s) 211, 242, 274, 277, 280, 290, 437
MfeI CAATTG 1 cut(s) 366
MflI RGATCY 1 cut(s) 114
MhlI GDGCHC 1 cut(s) 350
MlsI TGGCCA 1 cut(s) 800
MluCI AATT 7 cut(s) 221, 325, 366, 489, 550, 593, 995
MluNI TGGCCA 1 cut(s) 800
Mly113I GGCGCC 1 cut(s) 299
MlyI GAGTC 1 cut(s) 437
MnlI CCTC 5 cut(s) 104, 134, 297, 460, 646
Mox20I TGGCCA 1 cut(s) 800
MroXI GAANNNNTTC 1 cut(s) 125
MscI TGGCCA 1 cut(s) 800
MseI TTAA 7 cut(s) 170, 444, 498, 516, 540, 549, 765
Msp20I TGGCCA 1 cut(s) 800
MspA1I CMGCKG 2 cut(s) 29, 454
MspI CCGG 3 cut(s) 296, 406, 899
MspR9I CCNGG 3 cut(s) 296, 570, 730
MunI CAATTG 1 cut(s) 366
MvaI CCWGG 2 cut(s) 570, 730
MwoI GCNNNNNNNGC 3 cut(s) 371, 797, 875
NarI GGCGCC 1 cut(s) 299
NciI CCSGG 1 cut(s) 296
NcoI CCATGG 1 cut(s) 795
NdeII GATC 3 cut(s) 114, 813, 1031
NlaIII CATG 6 cut(s) 76, 107, 562, 724, 778, 799
NlaIV GGNNCC 1 cut(s) 300
NmeAIII GCCGAG 2 cut(s) 327, 940
NmuCI GTSAC 2 cut(s) 83, 732
NspI RCATGY 1 cut(s) 76
PcsI WCGNNNNNNNCGW 1 cut(s) 882
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 2 cut(s) 155, 448
PkrI GCNGC 2 cut(s) 373, 913
PleI GAGTC 1 cut(s) 436
PluTI GGCGCC 1 cut(s) 302
PpsI GAGTC 1 cut(s) 436
PshBI ATTAAT 1 cut(s) 549
PsiI TTATAA 1 cut(s) 941
Psp6I CCWGG 2 cut(s) 568, 728
PspGI CCWGG 2 cut(s) 568, 728
PspN4I GGNNCC 1 cut(s) 300
PsuI RGATCY 1 cut(s) 114
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 2 cut(s) 563, 856
RsaNI GTAC 2 cut(s) 562, 855
SaqAI TTAA 7 cut(s) 170, 444, 498, 516, 540, 549, 765
SatI GCNGC 2 cut(s) 372, 912
Sau3AI GATC 3 cut(s) 114, 813, 1031
SchI GAGTC 1 cut(s) 437
ScrFI CCNGG 3 cut(s) 296, 570, 730
SduI GDGCHC 1 cut(s) 350
SetI ASST 9 cut(s) 31, 96, 128, 145, 308, 347, 630, 731, 793
SexAI ACCWGGT 1 cut(s) 728
SfoI GGCGCC 1 cut(s) 300
Sse9I AATT 7 cut(s) 221, 325, 366, 489, 550, 593, 995
SsiI CCGC 5 cut(s) 68, 322, 454, 470, 911
SspDI GGCGCC 1 cut(s) 298
SspMI CTAG 3 cut(s) 486, 629, 771
StyD4I CCNGG 3 cut(s) 294, 568, 728
StyI CCWWGG 1 cut(s) 795
TaaI ACNGT 2 cut(s) 740, 1015
TaqI TCGA 4 cut(s) 149, 862, 885, 1007
TasI AATT 7 cut(s) 221, 325, 366, 489, 550, 593, 995
TatI WGTACW 2 cut(s) 561, 854
TauI GCSGC 1 cut(s) 914
TfiI GAWTC 2 cut(s) 155, 448
Tru1I TTAA 7 cut(s) 170, 444, 498, 516, 540, 549, 765
Tru9I TTAA 7 cut(s) 170, 444, 498, 516, 540, 549, 765
TscAI CASTG 1 cut(s) 970
TseFI GTSAC 2 cut(s) 83, 732
TseI GCWGC 1 cut(s) 371
Tsp45I GTSAC 2 cut(s) 83, 732
TspDTI ATGAA 5 cut(s) 291, 623, 704, 791, 1009
TspGWI ACGGA 1 cut(s) 806
TspRI CASTG 1 cut(s) 970
VspI ATTAAT 1 cut(s) 549
XapI RAATTY 1 cut(s) 221
XceI RCATGY 1 cut(s) 76
XcmI CCANNNNNNNNNTGG 1 cut(s) 502
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 3 cut(s) 486, 629, 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.