Rh6AG143000

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0008837
Physical Location & Seq
Reverse (-)
22216269 .. 22221438
5170 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG143000.1

Sequence Viewer

Length: 1128 bp
ATGAAGACCCTTACCTGCTTTACAGCTGAGATTCCTACGAAATATTCCTATAGCGAGTTAGTCTCCACAGCTGAATGGAAGTCATTTCAGGAACATTGCAAGGAAAACGCAGACTTGCAATCGTGGGACACAGAACTCAAAGAACATGCAGAAGAGATCTTGAAAACCGTCAAGAGTGAAGGAGGTCTCTCGATTGATTCCTGGAAATCAATCACACACTACTGGTGGTTTTCAAACAAGAAGATTGTGGAAGCAATCTTGGCATTCAAGAAAGGTATAACTGAAGGGGAGGGGAGTAGTAGGAAGAAGAAGAAAAATGAAGAAAGAGGGAACATCCTGACTGACGAGTATATTAAGAATAGCTCAAACATGCTGGATTTACTTTCTGAAATGGAAAACTTCCTCCTGCTTGCTCGTAATAGCCATTCATATATTGCAAAAATGATTCAACAGTACTTTGAGATACCCAATGGCATGAGTGGAGCTGAGTTCTACGGGAAGATTGAGGATAACTTAAAAAAGCTCAGGGGCTTTGATAATATCTTTGACCATTCTGCTGAAAACTTGCTCAAAAAGGCAGAAAGTTTGAAGAGTCAGTATGAAGAAATGCTTAGTAATTTGCAAGCCGAAATCTCTAAACTTGTTAAGAAGGAGAAACGCGCAAAATTATGGAAGAATGTCGCGTATATGATATTTGTAGGCGCTGCGGTTGGTTTGGCTATTGCCGGAATTGTGACTGTAGTTATAGCTGCTCCACCTGTTGCAGCAGCTATTACTTCTGCCATTGGCCCCATAGCAATGGCTGGAAACTGGACTGTCTCTTTGCTTGAAGAGTCTGAAAGAGCATTTAAGAATCAGCTGGAATTAAATGAGAAGATGCGTAAGGAAACTAGAATTTCCATTAAAGACTTGGGCAACATTAAGTCTTCGATTGATGGTGTTATTAACCAGATTAATTCAGTCATGTACAATCCAGGCTCTGAAATGGATCGGGAACAATTAGATTTCACAATGAAGGAAATCAAGAGCAAGCTGGGAGCGTTTCTGAATAAGGTTGAGGATTTGGAGAAGGACGTAGAAAAGACCATAAAGTATATAAGGAAGGCAAGGGATGACGTCATATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

375

Amino Acids

42.53

Weight (kDa)

6.59

Isoelectric Point (pI)

34.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 39 - 373 8.7e-41 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1119
Acc36I ACCTGC 1 cut(s) 23
AccII CGCG 2 cut(s) 660, 683
AciI CCGC 1 cut(s) 707
AclWI GGATC 1 cut(s) 996
AcsI RAATTY 1 cut(s) 894
AcuI CTGAAG 1 cut(s) 303
AcyI GRCGYC 1 cut(s) 1116
AfaI GTAC 2 cut(s) 455, 968
AgsI TTSAA 6 cut(s) 163, 234, 268, 449, 589, 830
AjnI CCWGG 2 cut(s) 200, 973
AluBI AGCT 9 cut(s) 26, 71, 363, 485, 523, 749, 770, 859, 1033
AluI AGCT 9 cut(s) 26, 71, 363, 485, 523, 749, 770, 859, 1033
Alw26I GTCTC 3 cut(s) 67, 191, 823
AlwI GGATC 1 cut(s) 996
AlwNI CAGNNNCTG 1 cut(s) 980
AoxI GGCC 1 cut(s) 787
ApeKI GCWGC 4 cut(s) 704, 749, 764, 767
ApoI RAATTY 1 cut(s) 894
ArsI GACNNNNNNTTYG 2 cut(s) 805, 837
AseI ATTAAT 1 cut(s) 954
AspLEI GCGC 2 cut(s) 662, 704
AspS9I GGNCC 1 cut(s) 788
BbsI GAAGAC 2 cut(s) 11, 918
BbvI GCAGC 4 cut(s) 691, 736, 776, 779
BccI CCATC 1 cut(s) 929
BciT130I CCWGG 2 cut(s) 202, 975
BcoDI GTCTC 3 cut(s) 67, 191, 823
BfaI CTAG 1 cut(s) 891
BfmI CTRYAG 2 cut(s) 49, 738
BfoI RGCGCY 1 cut(s) 705
BfuAI ACCTGC 1 cut(s) 23
BglII AGATCT 1 cut(s) 156
BisI GCNGC 4 cut(s) 705, 750, 765, 768
BlsI GCNGC 4 cut(s) 706, 751, 766, 769
BmcAI AGTACT 1 cut(s) 455
Bme1390I CCNGG 2 cut(s) 202, 975
BmgT120I GGNCC 1 cut(s) 788
BmiI GGNNCC 1 cut(s) 790
BmrFI CCNGG 2 cut(s) 202, 975
BmsI GCATC 1 cut(s) 867
BpiI GAAGAC 2 cut(s) 11, 918
BplI GAGNNNNNCTC 2 cut(s) 47, 79
Bpu10I CCTNAGC 1 cut(s) 524
BsaHI GRCGYC 1 cut(s) 1116
BsaI GGTCTC 1 cut(s) 191
Bse1I ACTGG 2 cut(s) 227, 815
Bse3DI GCAATG 2 cut(s) 94, 804
BseBI CCWGG 2 cut(s) 202, 975
BseGI GGATG 2 cut(s) 333, 1117
BseMI GCAATG 2 cut(s) 94, 804
BseMII CTCAG 3 cut(s) 18, 477, 538
BseNI ACTGG 2 cut(s) 227, 815
BseXI GCAGC 4 cut(s) 691, 736, 776, 779
BseYI CCCAGC 1 cut(s) 1033
Bsh1236I CGCG 2 cut(s) 660, 683
BshFI GGCC 1 cut(s) 789
BsiSI CCGG 1 cut(s) 726
BslFI GGGAC 1 cut(s) 140
BsmAI GTCTC 3 cut(s) 67, 191, 823
BsmFI GGGAC 1 cut(s) 140
BsmI GAATGC 1 cut(s) 263
BsnI GGCC 1 cut(s) 789
Bso31I GGTCTC 1 cut(s) 191
Bsp1407I TGTACA 1 cut(s) 966
Bsp143I GATC 2 cut(s) 156, 988
BspACI CCGC 1 cut(s) 707
BspANI GGCC 1 cut(s) 789
BspCNI CTCAG 3 cut(s) 19, 478, 537
BspFNI CGCG 2 cut(s) 660, 683
BspHI TCATGA 1 cut(s) 1124
BspLI GGNNCC 1 cut(s) 790
BspMI ACCTGC 1 cut(s) 23
BspPI GGATC 1 cut(s) 996
BspTNI GGTCTC 1 cut(s) 191
BsrDI GCAATG 2 cut(s) 94, 804
BsrGI TGTACA 1 cut(s) 966
BsrI ACTGG 2 cut(s) 227, 815
BssMI GATC 2 cut(s) 156, 988
BssNI GRCGYC 1 cut(s) 1116
Bst2UI CCWGG 2 cut(s) 202, 975
Bst4CI ACNGT 4 cut(s) 169, 453, 739, 817
Bst6I CTCTTC 3 cut(s) 147, 584, 825
BstACI GRCGYC 1 cut(s) 1116
BstAUI TGTACA 1 cut(s) 966
BstC8I GCNNGC 3 cut(s) 411, 624, 1031
BstDEI CTNAG 4 cut(s) 27, 486, 524, 611
BstF5I GGATG 2 cut(s) 333, 1117
BstFNI CGCG 2 cut(s) 660, 683
BstH2I RGCGCY 1 cut(s) 705
BstHHI GCGC 2 cut(s) 662, 704
BstKTI GATC 2 cut(s) 159, 991
BstMAI GTCTC 3 cut(s) 67, 191, 823
BstMBI GATC 2 cut(s) 156, 988
BstMWI GCNNNNNNNGC 1 cut(s) 260
BstNI CCWGG 2 cut(s) 202, 975
BstNSI RCATGY 2 cut(s) 149, 373
BstSCI CCNGG 2 cut(s) 200, 973
BstSFI CTRYAG 2 cut(s) 49, 738
BstUI CGCG 2 cut(s) 660, 683
BstV1I GCAGC 4 cut(s) 691, 736, 776, 779
BstV2I GAAGAC 2 cut(s) 11, 918
BstX2I RGATCY 1 cut(s) 156
BstXI CCANNNNNNTGG 1 cut(s) 799
BstYI RGATCY 1 cut(s) 156
BsuRI GGCC 1 cut(s) 789
BtsCI GGATG 2 cut(s) 333, 1117
BveI ACCTGC 1 cut(s) 23
Cac8I GCNNGC 3 cut(s) 411, 624, 1031
CaiI CAGNNNCTG 1 cut(s) 980
CciI TCATGA 1 cut(s) 1124
CfoI GCGC 2 cut(s) 662, 704
Cfr13I GGNCC 1 cut(s) 788
Csp6I GTAC 2 cut(s) 454, 967
CviAII CATG 5 cut(s) 146, 370, 475, 964, 1125
CviQI GTAC 2 cut(s) 454, 967
DdeI CTNAG 4 cut(s) 27, 486, 524, 611
DpnI GATC 2 cut(s) 158, 990
DpnII GATC 2 cut(s) 156, 988
Eam1104I CTCTTC 3 cut(s) 147, 584, 825
EarI CTCTTC 3 cut(s) 147, 584, 825
Eco31I GGTCTC 1 cut(s) 191
Eco57I CTGAAG 1 cut(s) 303
EcoRII CCWGG 2 cut(s) 200, 973
FaeI CATG 5 cut(s) 149, 373, 478, 967, 1128
FalI AAGNNNNNCTT 2 cut(s) 594, 626
FaqI GGGAC 1 cut(s) 140
FatI CATG 5 cut(s) 145, 369, 474, 963, 1124
Fnu4HI GCNGC 4 cut(s) 705, 750, 765, 768
FokI GGATG 2 cut(s) 320, 1124
Fsp4HI GCNGC 4 cut(s) 705, 750, 765, 768
FspBI CTAG 1 cut(s) 891
GlaI GCGC 2 cut(s) 661, 703
GluI GCNGC 4 cut(s) 705, 750, 765, 768
GsaI CCCAGC 1 cut(s) 1037
HaeII RGCGCY 1 cut(s) 705
HaeIII GGCC 1 cut(s) 789
HapII CCGG 1 cut(s) 726
HhaI GCGC 2 cut(s) 662, 704
Hin1I GRCGYC 1 cut(s) 1116
Hin1II CATG 5 cut(s) 149, 373, 478, 967, 1128
Hin6I GCGC 2 cut(s) 660, 702
HinP1I GCGC 2 cut(s) 660, 702
HinfI GANTC 6 cut(s) 31, 197, 445, 592, 833, 853
HpaII CCGG 1 cut(s) 726
Hpy188I TCNGA 4 cut(s) 388, 838, 982, 1047
Hpy188III TCNNGA 9 cut(s) 89, 160, 172, 190, 268, 337, 992, 1024, 1125
HpyAV CCTTC 6 cut(s) 173, 278, 643, 1009, 1063, 1096
HpyCH4III ACNGT 4 cut(s) 169, 453, 739, 817
HpyCH4IV ACGT 2 cut(s) 1074, 1116
HpyCH4V TGCA 6 cut(s) 99, 118, 149, 437, 622, 764
HpyF10VI GCNNNNNNNGC 1 cut(s) 260
HpyF3I CTNAG 4 cut(s) 27, 486, 524, 611
HpySE526I ACGT 2 cut(s) 1074, 1116
Hsp92I GRCGYC 1 cut(s) 1116
Hsp92II CATG 5 cut(s) 149, 373, 478, 967, 1128
HspAI GCGC 2 cut(s) 660, 702
Kzo9I GATC 2 cut(s) 156, 988
LmnI GCTCC 3 cut(s) 482, 757, 1037
Lsp1109I GCAGC 4 cut(s) 691, 736, 776, 779
LweI GCATC 1 cut(s) 867
MaeI CTAG 1 cut(s) 891
MaeII ACGT 2 cut(s) 1074, 1116
MaeIII GTNAC 1 cut(s) 733
MalI GATC 2 cut(s) 158, 990
MboI GATC 2 cut(s) 156, 988
MflI RGATCY 1 cut(s) 156
MluCI AATT 7 cut(s) 616, 665, 729, 863, 894, 955, 998
MlyI GAGTC 2 cut(s) 601, 842
MnlI CCTC 6 cut(s) 176, 283, 320, 413, 499, 1051
MseI TTAA 9 cut(s) 354, 515, 645, 849, 866, 903, 921, 945, 954
MslI CAYNNNNRTG 1 cut(s) 797
MspA1I CMGCKG 3 cut(s) 26, 71, 859
MspI CCGG 1 cut(s) 726
MspR9I CCNGG 2 cut(s) 202, 975
Mva1269I GAATGC 1 cut(s) 263
MvaI CCWGG 2 cut(s) 202, 975
MvnI CGCG 2 cut(s) 660, 683
MwoI GCNNNNNNNGC 1 cut(s) 260
NdeII GATC 2 cut(s) 156, 988
NlaIII CATG 5 cut(s) 149, 373, 478, 967, 1128
NlaIV GGNNCC 1 cut(s) 790
NmuCI GTSAC 1 cut(s) 733
NspI RCATGY 2 cut(s) 149, 373
PagI TCATGA 1 cut(s) 1124
PctI GAATGC 1 cut(s) 263
PfeI GAWTC 4 cut(s) 31, 197, 445, 853
PfoI TCCNGGA 1 cut(s) 200
PkrI GCNGC 4 cut(s) 706, 751, 766, 769
PleI GAGTC 2 cut(s) 600, 841
PpsI GAGTC 2 cut(s) 600, 841
PshBI ATTAAT 1 cut(s) 954
Psp6I CCWGG 2 cut(s) 200, 973
PspFI CCCAGC 1 cut(s) 1033
PspGI CCWGG 2 cut(s) 200, 973
PspN4I GGNNCC 1 cut(s) 790
PspPI GGNCC 1 cut(s) 788
PstNI CAGNNNCTG 1 cut(s) 980
PsuI RGATCY 1 cut(s) 156
PvuII CAGCTG 3 cut(s) 26, 71, 859
RsaI GTAC 2 cut(s) 455, 968
RsaNI GTAC 2 cut(s) 454, 967
RseI CAYNNNNRTG 1 cut(s) 797
SaqAI TTAA 9 cut(s) 354, 515, 645, 849, 866, 903, 921, 945, 954
SatI GCNGC 4 cut(s) 705, 750, 765, 768
Sau3AI GATC 2 cut(s) 156, 988
Sau96I GGNCC 1 cut(s) 788
ScaI AGTACT 1 cut(s) 455
SchI GAGTC 2 cut(s) 601, 842
ScrFI CCNGG 2 cut(s) 202, 975
SfaNI GCATC 1 cut(s) 867
SfcI CTRYAG 2 cut(s) 49, 738
SmiMI CAYNNNNRTG 1 cut(s) 797
Sse9I AATT 7 cut(s) 616, 665, 729, 863, 894, 955, 998
SsiI CCGC 1 cut(s) 707
SspI AATATT 1 cut(s) 44
SspMI CTAG 1 cut(s) 891
StyD4I CCNGG 2 cut(s) 200, 973
TaaI ACNGT 4 cut(s) 169, 453, 739, 817
TaiI ACGT 2 cut(s) 1077, 1119
TaqI TCGA 2 cut(s) 191, 929
TasI AATT 7 cut(s) 616, 665, 729, 863, 894, 955, 998
TatI WGTACW 2 cut(s) 453, 966
TfiI GAWTC 4 cut(s) 31, 197, 445, 853
Tru1I TTAA 9 cut(s) 354, 515, 645, 849, 866, 903, 921, 945, 954
Tru9I TTAA 9 cut(s) 354, 515, 645, 849, 866, 903, 921, 945, 954
TseFI GTSAC 1 cut(s) 733
TseI GCWGC 4 cut(s) 704, 749, 764, 767
Tsp45I GTSAC 1 cut(s) 733
TspDTI ATGAA 5 cut(s) 17, 333, 417, 615, 1028
VspI ATTAAT 1 cut(s) 954
XapI RAATTY 1 cut(s) 894
XceI RCATGY 2 cut(s) 149, 373
XcmI CCANNNNNNNNNTGG 1 cut(s) 907
XspI CTAG 1 cut(s) 891
ZraI GACGTC 1 cut(s) 1117
ZrmI AGTACT 1 cut(s) 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.