Rh6CG139800

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0008837
Physical Location & Seq
Reverse (-)
18068104 .. 18074460
6357 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG139800.1

Sequence Viewer

Length: 969 bp
ATGTTGAAGAGTACGTTCAATACCAAAAACGTCGAAGAAGACTCCAGATCGAAAGGACTCCGGCCGAAACTTCCCCACGGTAGCAGAGAGAGCACTGATCAGTTTGTTTTATCTCAAACCTTGAATCCCAAATTTTCCTACCTCATCGACCGCTCCAACTCCCTCCAAGTCTTTTATTCTTCGCAAAAGGGAAATAGAACGTGGACTTGTGGCCGGACAAACTTAGTCTCCACAGCTGAATGGAAGTCATTTCAGGAACATTGCAAGGAAAACGCAGACTTGCAATCGTGGGACACAGAACTCAAAGAACATGCAGAAGAGATCTTGAAAACCGTCAAGAGTGAAGGAGGTCTCTCGATTGATTCCTGGAAACCAATCACACACTACTGGTGGTGTTCAAACAAGAAGATTGTGGAAGCAATCTTGGCATTCAAGAAAGGTATAACTGAAGGGGAGGGGAGTAGTAGGAAGAAGAAGAAAAATGAAGAAAGAGGGAACATCCTGACTGACGAGTATATTAAGAATAGCTCAAACATGCTGGATTTACTTTCTGAAATGGAAAACTTCCTCCTGCTTGCTCGTAATAGCCATTCATATATTGCAAAAATGATTCAACAGTACTTTGAGATACCCAATGGCATGAGTGGAGCTGAGTTCTACGGGAAGATTGAGGATGACTTAAAAAAGCTCAGGGGCTCTGATAATATCTTTGACCATTCTGCTGAAAACTTGCTCAAAAAGGCAGAAAGTTTGAAGAGTCAGCATGAAGAAATGCTTCGTAATTTGCAAGCCGAAATCTCTAAACTTATTAAGAAGGAGAAACGCGCGAAATCATGGACGGACGTCACGTATATGATACTTGTAGGCACTGCGGTTGGTTTGGCTATTGCCGGAATTGTGACTGCAGTTATAGCTGCTCCACATGTTGCAGAAGCTATTACTTCTGCCATTGGCCCCATTACCCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

322

Amino Acids

36.38

Weight (kDa)

8.52

Isoelectric Point (pI)

40.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 94 - 316 6.1e-20 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 846
AccBSI CCGCTC 1 cut(s) 153
AccII CGCG 2 cut(s) 825, 827
AciI CCGC 2 cut(s) 151, 872
AcoI YGGCCR 2 cut(s) 62, 211
AcsI RAATTY 1 cut(s) 131
AcuI CTGAAG 1 cut(s) 468
AcyI GRCGYC 1 cut(s) 843
AfaI GTAC 2 cut(s) 13, 620
AflIII ACRYGT 1 cut(s) 922
AgsI TTSAA 8 cut(s) 7, 19, 124, 328, 399, 433, 614, 754
AjnI CCWGG 1 cut(s) 365
AluBI AGCT 6 cut(s) 236, 528, 650, 688, 914, 935
AluI AGCT 6 cut(s) 236, 528, 650, 688, 914, 935
Alw21I GWGCWC 1 cut(s) 95
Alw26I GTCTC 2 cut(s) 232, 356
AoxI GGCC 3 cut(s) 62, 211, 952
ApeKI GCWGC 1 cut(s) 914
ApoI RAATTY 1 cut(s) 131
Asp700I GAANNNNTTC 1 cut(s) 774
AspLEI GCGC 1 cut(s) 827
AspS9I GGNCC 1 cut(s) 953
BanII GRGCYC 1 cut(s) 698
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 95
BbvI GCAGC 1 cut(s) 901
BciT130I CCWGG 1 cut(s) 367
BclI TGATCA 1 cut(s) 97
BcoDI GTCTC 2 cut(s) 232, 356
BfmI CTRYAG 1 cut(s) 903
BglII AGATCT 1 cut(s) 321
BisI GCNGC 1 cut(s) 915
BlsI GCNGC 1 cut(s) 916
BmcAI AGTACT 1 cut(s) 620
Bme1390I CCNGG 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 953
BmiI GGNNCC 1 cut(s) 955
BmrFI CCNGG 1 cut(s) 367
BoxI GACNNNNGTC 1 cut(s) 842
BpiI GAAGAC 1 cut(s) 45
BpmI CTGGAG 1 cut(s) 28
Bpu10I CCTNAGC 1 cut(s) 689
BsaAI YACGTR 1 cut(s) 849
BsaHI GRCGYC 1 cut(s) 843
BsaI GGTCTC 1 cut(s) 356
BsaJI CCNNGG 1 cut(s) 76
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bse1I ACTGG 1 cut(s) 392
Bse3DI GCAATG 1 cut(s) 259
BseBI CCWGG 1 cut(s) 367
BseDI CCNNGG 1 cut(s) 76
BseGI GGATG 2 cut(s) 498, 679
BseMI GCAATG 1 cut(s) 259
BseMII CTCAG 2 cut(s) 642, 703
BseNI ACTGG 1 cut(s) 392
BseX3I CGGCCG 1 cut(s) 62
BseXI GCAGC 1 cut(s) 901
Bsh1236I CGCG 2 cut(s) 825, 827
Bsh1285I CGRYCG 2 cut(s) 65, 151
BshFI GGCC 3 cut(s) 64, 213, 954
BsiEI CGRYCG 2 cut(s) 65, 151
BsiHKAI GWGCWC 1 cut(s) 95
BsiSI CCGG 3 cut(s) 61, 214, 891
BslFI GGGAC 1 cut(s) 305
BsmAI GTCTC 2 cut(s) 232, 356
BsmFI GGGAC 1 cut(s) 305
BsmI GAATGC 1 cut(s) 428
BsnI GGCC 3 cut(s) 64, 213, 954
Bso31I GGTCTC 1 cut(s) 356
Bsp1286I GDGCHC 2 cut(s) 95, 698
Bsp143I GATC 3 cut(s) 47, 97, 321
BspACI CCGC 2 cut(s) 151, 872
BspANI GGCC 3 cut(s) 64, 213, 954
BspCNI CTCAG 2 cut(s) 643, 702
BspFNI CGCG 2 cut(s) 825, 827
BspLI GGNNCC 1 cut(s) 955
BspMAI CTGCAG 1 cut(s) 907
BspTNI GGTCTC 1 cut(s) 356
BsrBI CCGCTC 1 cut(s) 153
BsrDI GCAATG 1 cut(s) 259
BsrI ACTGG 1 cut(s) 392
BssECI CCNNGG 1 cut(s) 76
BssMI GATC 3 cut(s) 47, 97, 321
BssNI GRCGYC 1 cut(s) 843
Bst2UI CCWGG 1 cut(s) 367
Bst4CI ACNGT 3 cut(s) 80, 334, 618
Bst6I CTCTTC 3 cut(s) 2, 312, 749
BstACI GRCGYC 1 cut(s) 843
BstBAI YACGTR 1 cut(s) 849
BstC8I GCNNGC 2 cut(s) 576, 789
BstDEI CTNAG 3 cut(s) 223, 651, 689
BstDSI CCRYGG 1 cut(s) 76
BstF5I GGATG 2 cut(s) 498, 679
BstFNI CGCG 2 cut(s) 825, 827
BstHHI GCGC 1 cut(s) 827
BstKTI GATC 3 cut(s) 50, 100, 324
BstMAI GTCTC 2 cut(s) 232, 356
BstMBI GATC 3 cut(s) 47, 97, 321
BstMCI CGRYCG 2 cut(s) 65, 151
BstMWI GCNNNNNNNGC 3 cut(s) 90, 425, 911
BstNI CCWGG 1 cut(s) 367
BstNSI RCATGY 3 cut(s) 314, 538, 926
BstPAI GACNNNNGTC 1 cut(s) 842
BstSCI CCNGG 1 cut(s) 365
BstSFI CTRYAG 1 cut(s) 903
BstUI CGCG 2 cut(s) 825, 827
BstV1I GCAGC 1 cut(s) 901
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 1 cut(s) 321
BstYI RGATCY 1 cut(s) 321
BstZI CGGCCG 1 cut(s) 62
BsuRI GGCC 3 cut(s) 64, 213, 954
BtgI CCRYGG 1 cut(s) 76
BtsCI GGATG 2 cut(s) 498, 679
BtsI GCAGTG 1 cut(s) 867
BtsIMutI CAGTG 2 cut(s) 93, 867
Cac8I GCNNGC 2 cut(s) 576, 789
CfoI GCGC 1 cut(s) 827
Cfr13I GGNCC 1 cut(s) 953
Csp6I GTAC 2 cut(s) 12, 619
CviAII CATG 6 cut(s) 311, 535, 640, 764, 834, 923
CviQI GTAC 2 cut(s) 12, 619
DdeI CTNAG 3 cut(s) 223, 651, 689
DpnI GATC 3 cut(s) 49, 99, 323
DpnII GATC 3 cut(s) 47, 97, 321
EaeI YGGCCR 2 cut(s) 62, 211
EagI CGGCCG 1 cut(s) 62
Eam1104I CTCTTC 3 cut(s) 2, 312, 749
EarI CTCTTC 3 cut(s) 2, 312, 749
EclXI CGGCCG 1 cut(s) 62
Eco24I GRGCYC 1 cut(s) 698
Eco31I GGTCTC 1 cut(s) 356
Eco52I CGGCCG 1 cut(s) 62
Eco57I CTGAAG 1 cut(s) 468
EcoRII CCWGG 1 cut(s) 365
EcoT38I GRGCYC 1 cut(s) 698
FaeI CATG 6 cut(s) 314, 538, 643, 767, 837, 926
FalI AAGNNNNNCTT 2 cut(s) 759, 791
FaqI GGGAC 1 cut(s) 305
FatI CATG 6 cut(s) 310, 534, 639, 763, 833, 922
FbaI TGATCA 1 cut(s) 97
Fnu4HI GCNGC 1 cut(s) 915
FokI GGATG 2 cut(s) 485, 686
FriOI GRGCYC 1 cut(s) 698
Fsp4HI GCNGC 1 cut(s) 915
GlaI GCGC 1 cut(s) 826
GluI GCNGC 1 cut(s) 915
GsuI CTGGAG 1 cut(s) 28
HaeIII GGCC 3 cut(s) 64, 213, 954
HapII CCGG 3 cut(s) 61, 214, 891
HhaI GCGC 1 cut(s) 827
Hin1I GRCGYC 1 cut(s) 843
Hin1II CATG 6 cut(s) 314, 538, 643, 767, 837, 926
Hin6I GCGC 1 cut(s) 825
HinP1I GCGC 1 cut(s) 825
HinfI GANTC 6 cut(s) 41, 57, 124, 362, 610, 757
HpaII CCGG 3 cut(s) 61, 214, 891
Hpy166II GTNNAC 1 cut(s) 204
Hpy188I TCNGA 2 cut(s) 553, 700
Hpy188III TCNNGA 7 cut(s) 45, 254, 325, 337, 355, 433, 502
Hpy8I GTNNAC 1 cut(s) 204
Hpy99I CGWCG 1 cut(s) 35
HpyAV CCTTC 3 cut(s) 338, 443, 808
HpyCH4III ACNGT 3 cut(s) 80, 334, 618
HpyCH4IV ACGT 5 cut(s) 14, 30, 200, 843, 848
HpyCH4V TGCA 7 cut(s) 264, 283, 314, 602, 787, 905, 929
HpyF10VI GCNNNNNNNGC 3 cut(s) 90, 425, 911
HpyF3I CTNAG 3 cut(s) 223, 651, 689
HpySE526I ACGT 5 cut(s) 14, 30, 200, 843, 848
Hsp92I GRCGYC 1 cut(s) 843
Hsp92II CATG 6 cut(s) 314, 538, 643, 767, 837, 926
HspAI GCGC 1 cut(s) 825
Ksp22I TGATCA 1 cut(s) 97
Kzo9I GATC 3 cut(s) 47, 97, 321
LmnI GCTCC 3 cut(s) 158, 647, 922
Lsp1109I GCAGC 1 cut(s) 901
MaeII ACGT 5 cut(s) 14, 30, 200, 843, 848
MaeIII GTNAC 2 cut(s) 844, 898
MalI GATC 3 cut(s) 49, 99, 323
MbiI CCGCTC 1 cut(s) 153
MboI GATC 3 cut(s) 47, 97, 321
MflI RGATCY 1 cut(s) 321
MhlI GDGCHC 2 cut(s) 95, 698
MluCI AATT 3 cut(s) 131, 781, 894
MlyI GAGTC 3 cut(s) 35, 51, 766
MmeI TCCRAC 1 cut(s) 180
MnlI CCTC 7 cut(s) 152, 173, 341, 448, 485, 578, 664
MroXI GAANNNNTTC 1 cut(s) 774
MseI TTAA 3 cut(s) 519, 680, 810
MslI CAYNNNNRTG 1 cut(s) 851
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 3 cut(s) 61, 214, 891
MspR9I CCNGG 1 cut(s) 367
Mva1269I GAATGC 1 cut(s) 428
MvaI CCWGG 1 cut(s) 367
MvnI CGCG 2 cut(s) 825, 827
MwoI GCNNNNNNNGC 3 cut(s) 90, 425, 911
NdeII GATC 3 cut(s) 47, 97, 321
NlaIII CATG 6 cut(s) 314, 538, 643, 767, 837, 926
NlaIV GGNNCC 1 cut(s) 955
NmuCI GTSAC 2 cut(s) 844, 898
NspI RCATGY 3 cut(s) 314, 538, 926
PciI ACATGT 1 cut(s) 922
PcsI WCGNNNNNNNCGW 1 cut(s) 845
PctI GAATGC 1 cut(s) 428
PdmI GAANNNNTTC 1 cut(s) 774
PfeI GAWTC 3 cut(s) 124, 362, 610
PfoI TCCNGGA 1 cut(s) 365
PkrI GCNGC 1 cut(s) 916
PleI GAGTC 3 cut(s) 35, 51, 765
PpsI GAGTC 3 cut(s) 35, 51, 765
Ppu21I YACGTR 1 cut(s) 849
PscI ACATGT 1 cut(s) 922
PshAI GACNNNNGTC 1 cut(s) 842
Psp6I CCWGG 1 cut(s) 365
PspGI CCWGG 1 cut(s) 365
PspN4I GGNNCC 1 cut(s) 955
PspPI GGNCC 1 cut(s) 953
PstI CTGCAG 1 cut(s) 907
PsuI RGATCY 1 cut(s) 321
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 2 cut(s) 13, 620
RsaNI GTAC 2 cut(s) 12, 619
RseI CAYNNNNRTG 1 cut(s) 851
SaqAI TTAA 3 cut(s) 519, 680, 810
SatI GCNGC 1 cut(s) 915
Sau3AI GATC 3 cut(s) 47, 97, 321
Sau96I GGNCC 1 cut(s) 953
ScaI AGTACT 1 cut(s) 620
SchI GAGTC 3 cut(s) 35, 51, 766
ScrFI CCNGG 1 cut(s) 367
SduI GDGCHC 2 cut(s) 95, 698
SfcI CTRYAG 1 cut(s) 903
SmiMI CAYNNNNRTG 1 cut(s) 851
Sse9I AATT 3 cut(s) 131, 781, 894
SsiI CCGC 2 cut(s) 151, 872
StyD4I CCNGG 1 cut(s) 365
TaaI ACNGT 3 cut(s) 80, 334, 618
TaiI ACGT 5 cut(s) 17, 33, 203, 846, 851
TaqI TCGA 4 cut(s) 33, 50, 147, 356
TasI AATT 3 cut(s) 131, 781, 894
TatI WGTACW 1 cut(s) 618
TfiI GAWTC 3 cut(s) 124, 362, 610
Tru1I TTAA 3 cut(s) 519, 680, 810
Tru9I TTAA 3 cut(s) 519, 680, 810
TscAI CASTG 2 cut(s) 100, 874
TseFI GTSAC 2 cut(s) 844, 898
TseI GCWGC 1 cut(s) 914
Tsp45I GTSAC 2 cut(s) 844, 898
TspDTI ATGAA 3 cut(s) 498, 582, 780
TspGWI ACGGA 1 cut(s) 854
TspRI CASTG 2 cut(s) 100, 874
XapI RAATTY 1 cut(s) 131
XceI RCATGY 3 cut(s) 314, 538, 926
XmnI GAANNNNTTC 1 cut(s) 774
ZraI GACGTC 1 cut(s) 844
ZrmI AGTACT 1 cut(s) 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.