RLG00000014144

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008837
Physical Location & Seq
Forward (+)
48086494 .. 48087883
1390 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014144

Sequence Viewer

Length: 837 bp
ATGACCCTTGCCTGCTTTACAGCTGAGATTCCTACGAAATATTCCTATAGCGAGTTTGTCTCCACAGCTGAATGGAAGTCATTTCAGGAACATTGCAAGGAAAACACAGACTTGCAATGGTGGGACACAGAACTCAAAGAACATGTAGAAGAGATCTTGAAAACCGTCAAGAGTGAAGGAGGTCTCTCGATTGATTACTGGAAACCAATCACATACTACTGGTGGTGTTCAAACAAGAAGATTGTGGAAGAAATCTTGGCATTCAAGAAAGGTATAACTGAAGGGGAGGGGAGTAGTAGGAAGAAGAAGAAAAATGAAGAAAGAGGGAACATCCTGGCTGACAAGTATATTAAGAATAGCTCAAACATGCTGGATTTACTTTCTGAAATGGAAAGCTTCCTCCTGCATGCTCGAATTGTGACTGCAGTTATAGCTGCTCCACTTGTTGCAACAGCTATTACTTCTGCCATTGGCCCCATAGCAATGGCTAGACACTGGACTGTCTCTTTGTTTGAAGAGTCTGAAAGAGCATTTAAGAATCAGCGGGAATTAAATGAGGTGATGCGTAAGGAAACTAGAATTGCCATTAAAGAGTTGGGCGACATCAAGTCTTCGATTGATGGTGTTATTAACCAGATTAATTCAGTCATGTACAATCCAGGCTCTGAAATGGATCTGGAACAATTAGATTTCACAATGAAGGAAATCATGAGCAAGCTGGGAGCGTTTCTGAATAAGGTTGAGGATTTGGAGAAGGACGTAGAAAAGACCATAAAGTATATAAGGAAGGCAAGGGATGATGTTCTTATCATGAACACCCAGGTCACGCAGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

279

Amino Acids

31.98

Weight (kDa)

5.5

Isoelectric Point (pI)

27.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 126 - 269 1.9e-14 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 820
AciI CCGC 1 cut(s) 544
AclWI GGATC 1 cut(s) 681
AcuI CTGAAG 1 cut(s) 300
AfaI GTAC 1 cut(s) 653
AflIII ACRYGT 1 cut(s) 142
AgsI TTSAA 4 cut(s) 160, 231, 265, 515
AhdI GACNNNNNGTC 1 cut(s) 607
AjnI CCWGG 3 cut(s) 333, 658, 819
AluBI AGCT 7 cut(s) 23, 68, 360, 396, 434, 455, 718
AluI AGCT 7 cut(s) 23, 68, 360, 396, 434, 455, 718
Alw26I GTCTC 3 cut(s) 64, 188, 508
AlwI GGATC 1 cut(s) 681
AlwNI CAGNNNCTG 1 cut(s) 665
AoxI GGCC 1 cut(s) 472
ApeKI GCWGC 1 cut(s) 434
ArsI GACNNNNNNTTYG 2 cut(s) 490, 522
AseI ATTAAT 1 cut(s) 639
AspS9I GGNCC 1 cut(s) 473
AsuHPI GGTGA 1 cut(s) 571
BbsI GAAGAC 1 cut(s) 603
BbvI GCAGC 1 cut(s) 421
BccI CCATC 1 cut(s) 614
BciT130I CCWGG 3 cut(s) 335, 660, 821
BcoDI GTCTC 3 cut(s) 64, 188, 508
BfaI CTAG 2 cut(s) 489, 576
BfmI CTRYAG 2 cut(s) 46, 423
BfuAI ACCTGC 1 cut(s) 820
BglII AGATCT 1 cut(s) 153
BisI GCNGC 1 cut(s) 435
BlsI GCNGC 1 cut(s) 436
Bme1390I CCNGG 3 cut(s) 335, 660, 821
BmeRI GACNNNNNGTC 1 cut(s) 607
BmgT120I GGNCC 1 cut(s) 473
BmiI GGNNCC 1 cut(s) 475
BmrFI CCNGG 3 cut(s) 335, 660, 821
BmsI GCATC 1 cut(s) 552
BpiI GAAGAC 1 cut(s) 603
BplI GAGNNNNNCTC 2 cut(s) 44, 76
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 1 cut(s) 819
Bse1I ACTGG 3 cut(s) 203, 224, 500
Bse3DI GCAATG 3 cut(s) 91, 122, 489
BseBI CCWGG 3 cut(s) 335, 660, 821
BseDI CCNNGG 1 cut(s) 819
BseGI GGATG 2 cut(s) 330, 802
BseMI GCAATG 3 cut(s) 91, 122, 489
BseMII CTCAG 1 cut(s) 15
BseNI ACTGG 3 cut(s) 203, 224, 500
BseXI GCAGC 1 cut(s) 421
BseYI CCCAGC 1 cut(s) 718
BshFI GGCC 1 cut(s) 474
BslFI GGGAC 1 cut(s) 137
BsmAI GTCTC 3 cut(s) 64, 188, 508
BsmFI GGGAC 1 cut(s) 137
BsmI GAATGC 1 cut(s) 260
BsnI GGCC 1 cut(s) 474
Bso31I GGTCTC 1 cut(s) 188
Bsp1407I TGTACA 1 cut(s) 651
Bsp143I GATC 2 cut(s) 153, 673
BspACI CCGC 1 cut(s) 544
BspANI GGCC 1 cut(s) 474
BspCNI CTCAG 1 cut(s) 16
BspHI TCATGA 2 cut(s) 708, 810
BspLI GGNNCC 1 cut(s) 475
BspMAI CTGCAG 1 cut(s) 427
BspMI ACCTGC 1 cut(s) 820
BspPI GGATC 1 cut(s) 681
BspTNI GGTCTC 1 cut(s) 188
BsrDI GCAATG 3 cut(s) 91, 122, 489
BsrGI TGTACA 1 cut(s) 651
BsrI ACTGG 3 cut(s) 203, 224, 500
BssECI CCNNGG 1 cut(s) 819
BssMI GATC 2 cut(s) 153, 673
Bst2UI CCWGG 3 cut(s) 335, 660, 821
Bst4CI ACNGT 2 cut(s) 166, 502
Bst6I CTCTTC 2 cut(s) 144, 510
BstAUI TGTACA 1 cut(s) 651
BstC8I GCNNGC 3 cut(s) 13, 408, 716
BstDEI CTNAG 1 cut(s) 24
BstF5I GGATG 2 cut(s) 330, 802
BstKTI GATC 2 cut(s) 156, 676
BstMAI GTCTC 3 cut(s) 64, 188, 508
BstMBI GATC 2 cut(s) 153, 673
BstMWI GCNNNNNNNGC 1 cut(s) 431
BstNI CCWGG 3 cut(s) 335, 660, 821
BstNSI RCATGY 3 cut(s) 146, 370, 410
BstSCI CCNGG 3 cut(s) 333, 658, 819
BstSFI CTRYAG 2 cut(s) 46, 423
BstV1I GCAGC 1 cut(s) 421
BstV2I GAAGAC 1 cut(s) 603
BstX2I RGATCY 2 cut(s) 153, 673
BstXI CCANNNNNNTGG 1 cut(s) 484
BstYI RGATCY 2 cut(s) 153, 673
BsuRI GGCC 1 cut(s) 474
BtsCI GGATG 2 cut(s) 330, 802
BtsIMutI CAGTG 1 cut(s) 493
BveI ACCTGC 1 cut(s) 820
Cac8I GCNNGC 3 cut(s) 13, 408, 716
CaiI CAGNNNCTG 1 cut(s) 665
CciI TCATGA 2 cut(s) 708, 810
Cfr13I GGNCC 1 cut(s) 473
Csp6I GTAC 1 cut(s) 652
CviAII CATG 6 cut(s) 143, 367, 407, 649, 709, 811
CviQI GTAC 1 cut(s) 652
DdeI CTNAG 1 cut(s) 24
DpnI GATC 2 cut(s) 155, 675
DpnII GATC 2 cut(s) 153, 673
DriI GACNNNNNGTC 1 cut(s) 607
Eam1104I CTCTTC 2 cut(s) 144, 510
Eam1105I GACNNNNNGTC 1 cut(s) 607
EarI CTCTTC 2 cut(s) 144, 510
Eco31I GGTCTC 1 cut(s) 188
Eco57I CTGAAG 1 cut(s) 300
EcoRII CCWGG 3 cut(s) 333, 658, 819
FaeI CATG 6 cut(s) 146, 370, 410, 652, 712, 814
FaqI GGGAC 1 cut(s) 137
FatI CATG 6 cut(s) 142, 366, 406, 648, 708, 810
FauI CCCGC 1 cut(s) 537
Fnu4HI GCNGC 1 cut(s) 435
FokI GGATG 2 cut(s) 317, 809
Fsp4HI GCNGC 1 cut(s) 435
FspBI CTAG 2 cut(s) 489, 576
GluI GCNGC 1 cut(s) 435
GsaI CCCAGC 1 cut(s) 722
HaeIII GGCC 1 cut(s) 474
Hin1II CATG 6 cut(s) 146, 370, 410, 652, 712, 814
HindIII AAGCTT 1 cut(s) 394
HinfI GANTC 3 cut(s) 28, 518, 538
HphI GGTGA 1 cut(s) 571
Hpy188I TCNGA 4 cut(s) 385, 523, 667, 732
Hpy188III TCNNGA 8 cut(s) 86, 157, 169, 187, 265, 677, 709, 811
HpyAV CCTTC 5 cut(s) 170, 275, 694, 748, 781
HpyCH4III ACNGT 2 cut(s) 166, 502
HpyCH4IV ACGT 1 cut(s) 759
HpyCH4V TGCA 5 cut(s) 96, 115, 406, 425, 449
HpyF10VI GCNNNNNNNGC 1 cut(s) 431
HpyF3I CTNAG 1 cut(s) 24
HpySE526I ACGT 1 cut(s) 759
Hsp92II CATG 6 cut(s) 146, 370, 410, 652, 712, 814
Kzo9I GATC 2 cut(s) 153, 673
LmnI GCTCC 2 cut(s) 442, 722
Lsp1109I GCAGC 1 cut(s) 421
LweI GCATC 1 cut(s) 552
MaeI CTAG 2 cut(s) 489, 576
MaeII ACGT 1 cut(s) 759
MaeIII GTNAC 2 cut(s) 418, 823
MalI GATC 2 cut(s) 155, 675
MboI GATC 2 cut(s) 153, 673
MboII GAAGA 9 cut(s) 161, 250, 260, 313, 316, 319, 329, 527, 603
MflI RGATCY 2 cut(s) 153, 673
MluCI AATT 5 cut(s) 414, 548, 579, 640, 683
MlyI GAGTC 1 cut(s) 527
MnlI CCTC 6 cut(s) 173, 280, 317, 410, 550, 736
MseI TTAA 6 cut(s) 351, 534, 551, 588, 630, 639
MslI CAYNNNNRTG 1 cut(s) 482
MspA1I CMGCKG 3 cut(s) 23, 68, 544
MspR9I CCNGG 3 cut(s) 335, 660, 821
Mva1269I GAATGC 1 cut(s) 260
MvaI CCWGG 3 cut(s) 335, 660, 821
MwoI GCNNNNNNNGC 1 cut(s) 431
NdeII GATC 2 cut(s) 153, 673
NlaIII CATG 6 cut(s) 146, 370, 410, 652, 712, 814
NlaIV GGNNCC 1 cut(s) 475
NmuCI GTSAC 2 cut(s) 418, 823
NspI RCATGY 3 cut(s) 146, 370, 410
PaeI GCATGC 1 cut(s) 410
PagI TCATGA 2 cut(s) 708, 810
PciI ACATGT 1 cut(s) 142
PctI GAATGC 1 cut(s) 260
PfeI GAWTC 2 cut(s) 28, 538
PkrI GCNGC 1 cut(s) 436
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
PscI ACATGT 1 cut(s) 142
PshBI ATTAAT 1 cut(s) 639
Psp6I CCWGG 3 cut(s) 333, 658, 819
PspFI CCCAGC 1 cut(s) 718
PspGI CCWGG 3 cut(s) 333, 658, 819
PspN4I GGNNCC 1 cut(s) 475
PspPI GGNCC 1 cut(s) 473
PstI CTGCAG 1 cut(s) 427
PstNI CAGNNNCTG 1 cut(s) 665
PsuI RGATCY 2 cut(s) 153, 673
PvuII CAGCTG 2 cut(s) 23, 68
RsaI GTAC 1 cut(s) 653
RsaNI GTAC 1 cut(s) 652
RseI CAYNNNNRTG 1 cut(s) 482
SaqAI TTAA 6 cut(s) 351, 534, 551, 588, 630, 639
SatI GCNGC 1 cut(s) 435
Sau3AI GATC 2 cut(s) 153, 673
Sau96I GGNCC 1 cut(s) 473
SchI GAGTC 1 cut(s) 527
ScrFI CCNGG 3 cut(s) 335, 660, 821
SfaNI GCATC 1 cut(s) 552
SfcI CTRYAG 2 cut(s) 46, 423
SmiMI CAYNNNNRTG 1 cut(s) 482
SphI GCATGC 1 cut(s) 410
Sse9I AATT 5 cut(s) 414, 548, 579, 640, 683
SsiI CCGC 1 cut(s) 544
SspI AATATT 1 cut(s) 41
SspMI CTAG 2 cut(s) 489, 576
StyD4I CCNGG 3 cut(s) 333, 658, 819
TaaI ACNGT 2 cut(s) 166, 502
TaiI ACGT 1 cut(s) 762
TaqI TCGA 3 cut(s) 188, 412, 614
TasI AATT 5 cut(s) 414, 548, 579, 640, 683
TatI WGTACW 1 cut(s) 651
TfiI GAWTC 2 cut(s) 28, 538
Tru1I TTAA 6 cut(s) 351, 534, 551, 588, 630, 639
Tru9I TTAA 6 cut(s) 351, 534, 551, 588, 630, 639
TscAI CASTG 1 cut(s) 500
TseFI GTSAC 2 cut(s) 418, 823
TseI GCWGC 1 cut(s) 434
Tsp45I GTSAC 2 cut(s) 418, 823
TspDTI ATGAA 3 cut(s) 330, 713, 827
TspRI CASTG 1 cut(s) 500
VspI ATTAAT 1 cut(s) 639
XceI RCATGY 3 cut(s) 146, 370, 410
XcmI CCANNNNNNNNNTGG 1 cut(s) 592
XspI CTAG 2 cut(s) 489, 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.