Rroxscaffold_177G00434100

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000177
OG0008837
Physical Location & Seq
Reverse (-)
24763 .. 26402
1640 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_177G00434100.1

Sequence Viewer

Length: 1263 bp
ATGTTCGGTTGCGGTTGCGGTCGGTTGCGCCCGAAACCCTGCAACCCCGACCTCCTCCGACCTCAATCGCTGCCTAGACGCGTGCGGGAAGCCTCCGGCAAGCTCTCGTGCCGCCGTCGCTGCCTGGAACAGCCTGCTGCGTCGCCGTCCGCACTTTACCGAAAAAAGTTAGTCTCCACAGCCGAATGGAAGTCATTTCTGGAAGTTTGCAAGAAAAATGCAGACTTGCAATCGTGGGACACAGACCTCAAAACCCATGCAGAAGAAATCTTGAAAACCCTCAAGAGTGAAGGAGGACTCTCGATTGATTCCTGTAAACGAATCACACACTATGCGTGGGATTCAAACAAGAAGATTGTGCATGCAATCTTGGCACTCAAGGAAGGTATAACTAAAAGGAAGGGGAGTAGTAACAAGAAGAAGAGAAATGAAGAAAGGGTGAACATCCTGGCTGACGAATATATTAAGAATAGCTCAAACATGCTGGATTTCCTTTCTGAGATGGAAAACTTCCTCATGCTTGCTCGTAATAGCCATTCATATATTGCAACTGTGGTTCAACAGTACTTTGAGATACACAATGGTATGAGTGGAGCTGAGTCCTACGGGAAGATTGCGGATAACTTAAAAAAGCTCAAGGACTCTGATAATACCTTTGACCATTCTGCTGAAAACTTGCTCAAAAAGGCAGTAAGTTTGAAGAGTCAGCATGAAGAAATGCTTCATAATTTGCAAGGCGAAATCATTAAACTTAGTAAGAAGGAGAAACGCGCGAAATCATTGAAGAACGTCGCGTATATGATATTTGTAGGCGCTGTGGTTGGTTTGGCTATTGCCGGAATTGTGACTGCAGTTATAGCTGCTCCACCTGTTGCAGGAGCAATTGCTGCTGGCATTGTCCCCATAGCAATGGCTGGAAACTGGACTATCTATTGGTTTGAAGAGTCTGAAAAAGCATTTAAGAATCAGCGGGAAGTAAATGAGGCGAGGCGTAAGGAAACTAGAATTGCCATTAAAGAATTGGGTAACATTAAGTCTTCGATTGATGGTGTTATTAAACAGATTAATTCAGTCATGTACAATCCAAGCTCTGAAATGGATCGGGAACAATTAGATTTCAGAATGAAGGAAATCAAGAGCAAGCTGGGAGCGTTTCTGAATAAGGTTGAGGATTTGGAGAAGGACGTAGAAAAGACCAAAAAGGATATAAGGAAGGCAAGGGATGATGTTCATATCATGGACACCCAGGTCACGCAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

420

Amino Acids

47.34

Weight (kDa)

9.42

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 76 - 409 2.7e-43 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1247
AccII CGCG 4 cut(s) 81, 771, 773, 794
AciI CCGC 7 cut(s) 12, 18, 85, 112, 150, 617, 970
AclWI GGATC 1 cut(s) 1107
AfaI GTAC 2 cut(s) 566, 1079
AfiI CCNNNNNNNGG 1 cut(s) 875
AflIII ACRYGT 1 cut(s) 79
AgsI TTSAA 6 cut(s) 274, 345, 560, 700, 784, 941
AjnI CCWGG 3 cut(s) 123, 447, 1245
AluBI AGCT 7 cut(s) 103, 474, 596, 634, 860, 1089, 1144
AluI AGCT 7 cut(s) 103, 474, 596, 634, 860, 1089, 1144
Alw26I GTCTC 1 cut(s) 178
AlwI GGATC 1 cut(s) 1107
ApeKI GCWGC 5 cut(s) 70, 120, 137, 860, 887
AseI ATTAAT 1 cut(s) 1065
Asp700I GAANNNNTTC 1 cut(s) 720
AspLEI GCGC 3 cut(s) 30, 773, 815
AsuHPI GGTGA 1 cut(s) 451
BauI CACGAG 1 cut(s) 106
BbsI GAAGAC 1 cut(s) 1029
BbvI GCAGC 5 cut(s) 57, 107, 124, 847, 874
BccI CCATC 2 cut(s) 496, 1040
BceAI ACGGC 2 cut(s) 99, 130
BciT130I CCWGG 3 cut(s) 125, 449, 1247
BcoDI GTCTC 1 cut(s) 178
BfaI CTAG 2 cut(s) 75, 1002
BfmI CTRYAG 1 cut(s) 849
BfoI RGCGCY 1 cut(s) 816
BisI GCNGC 6 cut(s) 71, 112, 121, 138, 861, 888
BlsI GCNGC 6 cut(s) 72, 113, 122, 139, 862, 889
BmcAI AGTACT 1 cut(s) 566
Bme1390I CCNGG 3 cut(s) 125, 449, 1247
BmrFI CCNGG 3 cut(s) 125, 449, 1247
BpiI GAAGAC 1 cut(s) 1029
BpuEI CTTGAG 3 cut(s) 266, 362, 620
BsaJI CCNNGG 1 cut(s) 1245
Bsc4I CCNNNNNNNGG 1 cut(s) 875
Bse1I ACTGG 1 cut(s) 926
Bse3DI GCAATG 1 cut(s) 915
BseBI CCWGG 3 cut(s) 125, 449, 1247
BseDI CCNNGG 1 cut(s) 1245
BseGI GGATG 2 cut(s) 444, 1228
BseLI CCNNNNNNNGG 1 cut(s) 875
BseMI GCAATG 1 cut(s) 915
BseMII CTCAG 2 cut(s) 489, 588
BseNI ACTGG 1 cut(s) 926
BseRI GAGGAG 1 cut(s) 44
BseXI GCAGC 5 cut(s) 57, 107, 124, 847, 874
BseYI CCCAGC 1 cut(s) 1144
Bsh1236I CGCG 4 cut(s) 81, 771, 773, 794
Bsh1285I CGRYCG 1 cut(s) 22
BsiEI CGRYCG 1 cut(s) 22
BsiSI CCGG 2 cut(s) 96, 837
BslFI GGGAC 2 cut(s) 251, 884
BslI CCNNNNNNNGG 1 cut(s) 875
BsmAI GTCTC 1 cut(s) 178
BsmFI GGGAC 2 cut(s) 251, 884
Bsp1407I TGTACA 1 cut(s) 1077
Bsp143I GATC 1 cut(s) 1099
BspACI CCGC 7 cut(s) 12, 18, 85, 112, 150, 617, 970
BspCNI CTCAG 2 cut(s) 490, 589
BspFNI CGCG 4 cut(s) 81, 771, 773, 794
BspMAI CTGCAG 1 cut(s) 853
BspPI GGATC 1 cut(s) 1107
BsrDI GCAATG 1 cut(s) 915
BsrGI TGTACA 1 cut(s) 1077
BsrI ACTGG 1 cut(s) 926
BssECI CCNNGG 1 cut(s) 1245
BssMI GATC 1 cut(s) 1099
BssSI CACGAG 1 cut(s) 106
Bst2BI CACGAG 1 cut(s) 106
Bst2UI CCWGG 3 cut(s) 125, 449, 1247
Bst4CI ACNGT 2 cut(s) 553, 564
Bst6I CTCTTC 3 cut(s) 416, 695, 936
BstAPI GCANNNNNTGC 1 cut(s) 887
BstAUI TGTACA 1 cut(s) 1077
BstC8I GCNNGC 7 cut(s) 83, 101, 135, 363, 522, 892, 1142
BstDEI CTNAG 3 cut(s) 498, 597, 752
BstENI CCTNNNNNAGG 1 cut(s) 873
BstF5I GGATG 2 cut(s) 444, 1228
BstFNI CGCG 4 cut(s) 81, 771, 773, 794
BstH2I RGCGCY 1 cut(s) 816
BstHHI GCGC 3 cut(s) 30, 773, 815
BstKTI GATC 1 cut(s) 1102
BstMAI GTCTC 1 cut(s) 178
BstMBI GATC 1 cut(s) 1099
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 5 cut(s) 117, 120, 371, 857, 887
BstNI CCWGG 3 cut(s) 125, 449, 1247
BstNSI RCATGY 2 cut(s) 365, 484
BstSCI CCNGG 3 cut(s) 123, 447, 1245
BstSFI CTRYAG 1 cut(s) 849
BstUI CGCG 4 cut(s) 81, 771, 773, 794
BstV1I GCAGC 5 cut(s) 57, 107, 124, 847, 874
BstV2I GAAGAC 1 cut(s) 1029
BstXI CCANNNNNNTGG 1 cut(s) 910
BtsCI GGATG 2 cut(s) 444, 1228
Cac8I GCNNGC 7 cut(s) 83, 101, 135, 363, 522, 892, 1142
CfoI GCGC 3 cut(s) 30, 773, 815
CseI GACGC 2 cut(s) 87, 129
Csp6I GTAC 2 cut(s) 565, 1078
CviAII CATG 7 cut(s) 257, 362, 481, 517, 710, 1075, 1237
CviQI GTAC 2 cut(s) 565, 1078
DdeI CTNAG 3 cut(s) 498, 597, 752
DpnI GATC 1 cut(s) 1101
DpnII GATC 1 cut(s) 1099
DrdI GACNNNNNNGTC 1 cut(s) 1247
DseDI GACNNNNNNGTC 1 cut(s) 1247
Eam1104I CTCTTC 3 cut(s) 416, 695, 936
EarI CTCTTC 3 cut(s) 416, 695, 936
EcoNI CCTNNNNNAGG 1 cut(s) 873
EcoRII CCWGG 3 cut(s) 123, 447, 1245
FaeI CATG 7 cut(s) 260, 365, 484, 520, 713, 1078, 1240
FalI AAGNNNNNCTT 2 cut(s) 705, 737
FaqI GGGAC 2 cut(s) 251, 884
FatI CATG 7 cut(s) 256, 361, 480, 516, 709, 1074, 1236
FauI CCCGC 2 cut(s) 78, 963
Fnu4HI GCNGC 6 cut(s) 71, 112, 121, 138, 861, 888
FokI GGATG 2 cut(s) 431, 1235
Fsp4HI GCNGC 6 cut(s) 71, 112, 121, 138, 861, 888
FspBI CTAG 2 cut(s) 75, 1002
GlaI GCGC 3 cut(s) 29, 772, 814
GluI GCNGC 6 cut(s) 71, 112, 121, 138, 861, 888
GsaI CCCAGC 1 cut(s) 1148
HaeII RGCGCY 1 cut(s) 816
HapII CCGG 2 cut(s) 96, 837
HgaI GACGC 2 cut(s) 87, 129
HhaI GCGC 3 cut(s) 30, 773, 815
Hin1II CATG 7 cut(s) 260, 365, 484, 520, 713, 1078, 1240
Hin6I GCGC 3 cut(s) 28, 771, 813
HinP1I GCGC 3 cut(s) 28, 771, 813
HinfI GANTC 9 cut(s) 297, 308, 321, 341, 599, 641, 703, 944, 964
HpaII CCGG 2 cut(s) 96, 837
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 2 cut(s) 317, 442
Hpy188I TCNGA 7 cut(s) 59, 499, 646, 949, 1093, 1121, 1158
Hpy188III TCNNGA 7 cut(s) 200, 271, 283, 301, 1103, 1135, 1260
Hpy8I GTNNAC 2 cut(s) 317, 442
Hpy99I CGWCG 3 cut(s) 120, 145, 794
HpyAV CCTTC 7 cut(s) 284, 377, 394, 754, 1120, 1174, 1207
HpyCH4III ACNGT 2 cut(s) 553, 564
HpyCH4IV ACGT 2 cut(s) 789, 1185
HpyF10VI GCNNNNNNNGC 5 cut(s) 117, 120, 371, 857, 887
HpyF3I CTNAG 3 cut(s) 498, 597, 752
HpySE526I ACGT 2 cut(s) 789, 1185
Hsp92II CATG 7 cut(s) 260, 365, 484, 520, 713, 1078, 1240
HspAI GCGC 3 cut(s) 28, 771, 813
Kzo9I GATC 1 cut(s) 1099
LmnI GCTCC 4 cut(s) 593, 868, 878, 1148
Lsp1109I GCAGC 5 cut(s) 57, 107, 124, 847, 874
MaeI CTAG 2 cut(s) 75, 1002
MaeII ACGT 2 cut(s) 789, 1185
MaeIII GTNAC 4 cut(s) 410, 844, 1025, 1249
MalI GATC 1 cut(s) 1101
MboI GATC 1 cut(s) 1099
MfeI CAATTG 1 cut(s) 882
MluCI AATT 7 cut(s) 727, 840, 882, 1005, 1019, 1066, 1109
MluI ACGCGT 1 cut(s) 79
MlyI GAGTC 5 cut(s) 291, 608, 635, 712, 953
MmeI TCCRAC 1 cut(s) 82
MroXI GAANNNNTTC 1 cut(s) 720
MseI TTAA 8 cut(s) 465, 626, 747, 960, 1014, 1032, 1056, 1065
MslI CAYNNNNRTG 1 cut(s) 908
MspA1I CMGCKG 1 cut(s) 970
MspI CCGG 2 cut(s) 96, 837
MspR9I CCNGG 3 cut(s) 125, 449, 1247
MunI CAATTG 1 cut(s) 882
MvaI CCWGG 3 cut(s) 125, 449, 1247
MvnI CGCG 4 cut(s) 81, 771, 773, 794
MwoI GCNNNNNNNGC 5 cut(s) 117, 120, 371, 857, 887
NdeII GATC 1 cut(s) 1099
NlaIII CATG 7 cut(s) 260, 365, 484, 520, 713, 1078, 1240
NmuCI GTSAC 2 cut(s) 844, 1249
NspI RCATGY 2 cut(s) 365, 484
PaeI GCATGC 1 cut(s) 365
PdmI GAANNNNTTC 1 cut(s) 720
PfeI GAWTC 4 cut(s) 308, 321, 341, 964
PkrI GCNGC 6 cut(s) 72, 113, 122, 139, 862, 889
PleI GAGTC 5 cut(s) 291, 607, 635, 711, 952
PpsI GAGTC 5 cut(s) 291, 607, 635, 711, 952
PshBI ATTAAT 1 cut(s) 1065
Psp6I CCWGG 3 cut(s) 123, 447, 1245
PspFI CCCAGC 1 cut(s) 1144
PspGI CCWGG 3 cut(s) 123, 447, 1245
PstI CTGCAG 1 cut(s) 853
RsaI GTAC 2 cut(s) 566, 1079
RsaNI GTAC 2 cut(s) 565, 1078
RseI CAYNNNNRTG 1 cut(s) 908
SaqAI TTAA 8 cut(s) 465, 626, 747, 960, 1014, 1032, 1056, 1065
SatI GCNGC 6 cut(s) 71, 112, 121, 138, 861, 888
Sau3AI GATC 1 cut(s) 1099
ScaI AGTACT 1 cut(s) 566
SchI GAGTC 5 cut(s) 291, 608, 635, 712, 953
ScrFI CCNGG 3 cut(s) 125, 449, 1247
SfcI CTRYAG 1 cut(s) 849
SmiMI CAYNNNNRTG 1 cut(s) 908
SmlI CTYRAG 3 cut(s) 281, 377, 635
SmoI CTYRAG 3 cut(s) 281, 377, 635
SphI GCATGC 1 cut(s) 365
Sse9I AATT 7 cut(s) 727, 840, 882, 1005, 1019, 1066, 1109
SsiI CCGC 7 cut(s) 12, 18, 85, 112, 150, 617, 970
SspMI CTAG 2 cut(s) 75, 1002
StyD4I CCNGG 3 cut(s) 123, 447, 1245
TaaI ACNGT 2 cut(s) 553, 564
TaiI ACGT 2 cut(s) 792, 1188
TaqI TCGA 2 cut(s) 302, 1040
TasI AATT 7 cut(s) 727, 840, 882, 1005, 1019, 1066, 1109
TatI WGTACW 2 cut(s) 564, 1077
TauI GCSGC 1 cut(s) 114
TfiI GAWTC 4 cut(s) 308, 321, 341, 964
Tru1I TTAA 8 cut(s) 465, 626, 747, 960, 1014, 1032, 1056, 1065
Tru9I TTAA 8 cut(s) 465, 626, 747, 960, 1014, 1032, 1056, 1065
TseFI GTSAC 2 cut(s) 844, 1249
TseI GCWGC 5 cut(s) 70, 120, 137, 860, 887
Tsp45I GTSAC 2 cut(s) 844, 1249
TspDTI ATGAA 6 cut(s) 444, 528, 713, 726, 1139, 1220
VspI ATTAAT 1 cut(s) 1065
XagI CCTNNNNNAGG 1 cut(s) 873
XceI RCATGY 2 cut(s) 365, 484
XcmI CCANNNNNNNNNTGG 1 cut(s) 1018
XmnI GAANNNNTTC 1 cut(s) 720
XspI CTAG 2 cut(s) 75, 1002
ZrmI AGTACT 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.