Rroxscaffold_7G00204890

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008837
Physical Location & Seq
Reverse (-)
54374753 .. 54376409
1657 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00204890.1

Sequence Viewer

Length: 1287 bp
ATGAGGTTGCAGCGCCGCATGTTCGGTTGCGGTTGCAGGTCGGGTTGCGCCGAAACCTGCAACCCCGACCTCCTCCGACCTCAATCGCTGCCTAGACGCGTGCGGGAAGCCTCCGGCAAGCTCGTGCCGCCGTCGCTGCCTGGAACAGCCTGCTGCGTCGCCGTCCGCACTTTACCGAAAGTGCGGACAAAGTTAGTCTCCACAGCCGAATGGAAGTCATTTCTGGAAGTTTGCAAGAAAAATGCAGACTTGCAATCGTGGGACACAGACCTCAAAACCCATGCAGAAGAAATCTTGAAAACCCTCAAGAGTGAAGGAGGACTCTCGATTGATTCCTGTAAACGAATCACACACTATGCGTGGGATTCAAACAAGAAGATTGTGCATGCAATCTTGGCACTCAAGGAAGGTATAACTAAAAGGAAGGGGAGTAGTAACAAGAAGAAGAGAAATGAAGAAAGGGTGAACATCCTGGCTGACGAATATATTAAGAATAGCTCAAACATGCTGGATTTCCTTTCTGAGATGGAAAACTTCCTCATGCTTGCTCGTAATAGCCATTCATATATTGCAACTGTGGTTCAACAGTACTTTGAGATACACAATGGTATGAGTGGAGCTGAGTCCTACGGGAAGATTGCGGATAACTTAAAAAAGCTCAAGGACTCTGATAATACCTTTGACCATTCTGCTGAAAACTTGCTCAAAAAGGCAGTAAGTTTGAAGAGTCAGCATGAAGAAATGCTTCATAATTTGCAAGGCGAAATCATTAAACTTAGTAAGAAGGAGAAACGCGCGAAATCATTGAAGAACGTCGCGTATATGATATTTGTAGGCGCTGTGGTTGGTTTGGCTATTGCCGGAATTGTGACTGCAGTTATAGCTGCTCCACCTGTTGCAGGAGCAATTGCTGCTGGCATTGTCCCCATAGCAATGGCTGGAAACTGGACTATCTATTGGTTTGAAGAGTCTGAAAAAGCATTTAAGAATCAGCGGGAAGTAAATGAGGCGAGGCGTAAGGAAACTAGAATTGCCATTAAAGAATTGGGTAACATTAAGTCTTCGATTGATGGTGTTATTAAACAGATTAATTCAGTCATGTACAATCCAAGCTCTGAAATGGATCGGGAACAATTAGATTTCAGAATGAAGGAAATCAAGAGCAAGCTGGGAGCGTTTCTGAATAAGGTTGAGGATTTGGAGAAGGACGTAGAAAAGACCAAAAAGGATATAAGGAAGGCAAGGGATGATGTTCATATCATGGACACCCAGGTCACGCAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

428

Amino Acids

48.06

Weight (kDa)

9.37

Isoelectric Point (pI)

42.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 84 - 417 2.9e-43 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1271
Acc36I ACCTGC 2 cut(s) 27, 65
AccII CGCG 4 cut(s) 99, 795, 797, 818
AciI CCGC 8 cut(s) 16, 30, 103, 128, 166, 184, 641, 994
AclWI GGATC 1 cut(s) 1131
AfaI GTAC 2 cut(s) 590, 1103
AfiI CCNNNNNNNGG 1 cut(s) 899
AflIII ACRYGT 1 cut(s) 97
AgsI TTSAA 6 cut(s) 298, 369, 584, 724, 808, 965
AjnI CCWGG 3 cut(s) 139, 471, 1269
AluBI AGCT 7 cut(s) 121, 498, 620, 658, 884, 1113, 1168
AluI AGCT 7 cut(s) 121, 498, 620, 658, 884, 1113, 1168
Alw26I GTCTC 1 cut(s) 202
AlwI GGATC 1 cut(s) 1131
ApeKI GCWGC 6 cut(s) 10, 88, 136, 153, 884, 911
AseI ATTAAT 1 cut(s) 1089
Asp700I GAANNNNTTC 1 cut(s) 744
AspLEI GCGC 4 cut(s) 15, 50, 797, 839
AsuHPI GGTGA 1 cut(s) 475
BauI CACGAG 1 cut(s) 122
BbsI GAAGAC 1 cut(s) 1053
BbvI GCAGC 6 cut(s) 22, 75, 123, 140, 871, 898
BccI CCATC 2 cut(s) 520, 1064
BceAI ACGGC 2 cut(s) 115, 146
BciT130I CCWGG 3 cut(s) 141, 473, 1271
BcoDI GTCTC 1 cut(s) 202
BfaI CTAG 2 cut(s) 93, 1026
BfmI CTRYAG 1 cut(s) 873
BfoI RGCGCY 2 cut(s) 16, 840
BfuAI ACCTGC 2 cut(s) 27, 65
BisI GCNGC 8 cut(s) 11, 16, 89, 128, 137, 154, 885, 912
BlsI GCNGC 8 cut(s) 12, 17, 90, 129, 138, 155, 886, 913
BmcAI AGTACT 1 cut(s) 590
Bme1390I CCNGG 3 cut(s) 141, 473, 1271
BmrFI CCNGG 3 cut(s) 141, 473, 1271
BpiI GAAGAC 1 cut(s) 1053
BpuEI CTTGAG 3 cut(s) 290, 386, 644
BsaJI CCNNGG 1 cut(s) 1269
Bsc4I CCNNNNNNNGG 1 cut(s) 899
Bse1I ACTGG 1 cut(s) 950
Bse3DI GCAATG 1 cut(s) 939
BseBI CCWGG 3 cut(s) 141, 473, 1271
BseDI CCNNGG 1 cut(s) 1269
BseGI GGATG 2 cut(s) 468, 1252
BseLI CCNNNNNNNGG 1 cut(s) 899
BseMI GCAATG 1 cut(s) 939
BseMII CTCAG 2 cut(s) 513, 612
BseNI ACTGG 1 cut(s) 950
BseRI GAGGAG 1 cut(s) 62
BseXI GCAGC 6 cut(s) 22, 75, 123, 140, 871, 898
BseYI CCCAGC 1 cut(s) 1168
Bsh1236I CGCG 4 cut(s) 99, 795, 797, 818
BsiSI CCGG 2 cut(s) 114, 861
BslFI GGGAC 2 cut(s) 275, 908
BslI CCNNNNNNNGG 1 cut(s) 899
BsmAI GTCTC 1 cut(s) 202
BsmFI GGGAC 2 cut(s) 275, 908
Bsp1407I TGTACA 1 cut(s) 1101
Bsp143I GATC 1 cut(s) 1123
BspACI CCGC 8 cut(s) 16, 30, 103, 128, 166, 184, 641, 994
BspCNI CTCAG 2 cut(s) 514, 613
BspFNI CGCG 4 cut(s) 99, 795, 797, 818
BspMAI CTGCAG 1 cut(s) 877
BspMI ACCTGC 2 cut(s) 27, 65
BspPI GGATC 1 cut(s) 1131
BsrDI GCAATG 1 cut(s) 939
BsrGI TGTACA 1 cut(s) 1101
BsrI ACTGG 1 cut(s) 950
BssECI CCNNGG 1 cut(s) 1269
BssMI GATC 1 cut(s) 1123
BssSI CACGAG 1 cut(s) 122
Bst2BI CACGAG 1 cut(s) 122
Bst2UI CCWGG 3 cut(s) 141, 473, 1271
Bst4CI ACNGT 2 cut(s) 577, 588
Bst6I CTCTTC 3 cut(s) 440, 719, 960
BstAPI GCANNNNNTGC 1 cut(s) 911
BstAUI TGTACA 1 cut(s) 1101
BstC8I GCNNGC 7 cut(s) 101, 119, 151, 387, 546, 916, 1166
BstDEI CTNAG 3 cut(s) 522, 621, 776
BstENI CCTNNNNNAGG 1 cut(s) 897
BstF5I GGATG 2 cut(s) 468, 1252
BstFNI CGCG 4 cut(s) 99, 795, 797, 818
BstH2I RGCGCY 2 cut(s) 16, 840
BstHHI GCGC 4 cut(s) 15, 50, 797, 839
BstKTI GATC 1 cut(s) 1126
BstMAI GTCTC 1 cut(s) 202
BstMBI GATC 1 cut(s) 1123
BstMWI GCNNNNNNNGC 6 cut(s) 127, 133, 136, 395, 881, 911
BstNI CCWGG 3 cut(s) 141, 473, 1271
BstNSI RCATGY 3 cut(s) 22, 389, 508
BstSCI CCNGG 3 cut(s) 139, 471, 1269
BstSFI CTRYAG 1 cut(s) 873
BstUI CGCG 4 cut(s) 99, 795, 797, 818
BstV1I GCAGC 6 cut(s) 22, 75, 123, 140, 871, 898
BstV2I GAAGAC 1 cut(s) 1053
BstXI CCANNNNNNTGG 1 cut(s) 934
BtsCI GGATG 2 cut(s) 468, 1252
BveI ACCTGC 2 cut(s) 27, 65
Cac8I GCNNGC 7 cut(s) 101, 119, 151, 387, 546, 916, 1166
CfoI GCGC 4 cut(s) 15, 50, 797, 839
CseI GACGC 2 cut(s) 105, 145
Csp6I GTAC 2 cut(s) 589, 1102
CviAII CATG 8 cut(s) 19, 281, 386, 505, 541, 734, 1099, 1261
CviQI GTAC 2 cut(s) 589, 1102
DdeI CTNAG 3 cut(s) 522, 621, 776
DpnI GATC 1 cut(s) 1125
DpnII GATC 1 cut(s) 1123
DrdI GACNNNNNNGTC 1 cut(s) 1271
DseDI GACNNNNNNGTC 1 cut(s) 1271
Eam1104I CTCTTC 3 cut(s) 440, 719, 960
EarI CTCTTC 3 cut(s) 440, 719, 960
EcoNI CCTNNNNNAGG 1 cut(s) 897
EcoRII CCWGG 3 cut(s) 139, 471, 1269
FaeI CATG 8 cut(s) 22, 284, 389, 508, 544, 737, 1102, 1264
FalI AAGNNNNNCTT 2 cut(s) 729, 761
FaqI GGGAC 2 cut(s) 275, 908
FatI CATG 8 cut(s) 18, 280, 385, 504, 540, 733, 1098, 1260
FauI CCCGC 2 cut(s) 96, 987
Fnu4HI GCNGC 8 cut(s) 11, 16, 89, 128, 137, 154, 885, 912
FokI GGATG 2 cut(s) 455, 1259
Fsp4HI GCNGC 8 cut(s) 11, 16, 89, 128, 137, 154, 885, 912
FspBI CTAG 2 cut(s) 93, 1026
GlaI GCGC 4 cut(s) 14, 49, 796, 838
GluI GCNGC 8 cut(s) 11, 16, 89, 128, 137, 154, 885, 912
GsaI CCCAGC 1 cut(s) 1172
HaeII RGCGCY 2 cut(s) 16, 840
HapII CCGG 2 cut(s) 114, 861
HgaI GACGC 2 cut(s) 105, 145
HhaI GCGC 4 cut(s) 15, 50, 797, 839
Hin1II CATG 8 cut(s) 22, 284, 389, 508, 544, 737, 1102, 1264
Hin6I GCGC 4 cut(s) 13, 48, 795, 837
HinP1I GCGC 4 cut(s) 13, 48, 795, 837
HinfI GANTC 9 cut(s) 321, 332, 345, 365, 623, 665, 727, 968, 988
HpaII CCGG 2 cut(s) 114, 861
HphI GGTGA 1 cut(s) 475
Hpy166II GTNNAC 2 cut(s) 341, 466
Hpy188I TCNGA 7 cut(s) 77, 523, 670, 973, 1117, 1145, 1182
Hpy188III TCNNGA 7 cut(s) 224, 295, 307, 325, 1127, 1159, 1284
Hpy8I GTNNAC 2 cut(s) 341, 466
Hpy99I CGWCG 3 cut(s) 136, 161, 818
HpyAV CCTTC 7 cut(s) 308, 401, 418, 778, 1144, 1198, 1231
HpyCH4III ACNGT 2 cut(s) 577, 588
HpyCH4IV ACGT 2 cut(s) 813, 1209
HpyF10VI GCNNNNNNNGC 6 cut(s) 127, 133, 136, 395, 881, 911
HpyF3I CTNAG 3 cut(s) 522, 621, 776
HpySE526I ACGT 2 cut(s) 813, 1209
Hsp92II CATG 8 cut(s) 22, 284, 389, 508, 544, 737, 1102, 1264
HspAI GCGC 4 cut(s) 13, 48, 795, 837
Kzo9I GATC 1 cut(s) 1123
LmnI GCTCC 4 cut(s) 617, 892, 902, 1172
Lsp1109I GCAGC 6 cut(s) 22, 75, 123, 140, 871, 898
MaeI CTAG 2 cut(s) 93, 1026
MaeII ACGT 2 cut(s) 813, 1209
MaeIII GTNAC 4 cut(s) 434, 868, 1049, 1273
MalI GATC 1 cut(s) 1125
MboI GATC 1 cut(s) 1123
MfeI CAATTG 1 cut(s) 906
MluCI AATT 7 cut(s) 751, 864, 906, 1029, 1043, 1090, 1133
MluI ACGCGT 1 cut(s) 97
MlyI GAGTC 5 cut(s) 315, 632, 659, 736, 977
MmeI TCCRAC 1 cut(s) 100
MroXI GAANNNNTTC 1 cut(s) 744
MseI TTAA 8 cut(s) 489, 650, 771, 984, 1038, 1056, 1080, 1089
MslI CAYNNNNRTG 1 cut(s) 932
MspA1I CMGCKG 1 cut(s) 994
MspI CCGG 2 cut(s) 114, 861
MspR9I CCNGG 3 cut(s) 141, 473, 1271
MunI CAATTG 1 cut(s) 906
MvaI CCWGG 3 cut(s) 141, 473, 1271
MvnI CGCG 4 cut(s) 99, 795, 797, 818
MwoI GCNNNNNNNGC 6 cut(s) 127, 133, 136, 395, 881, 911
NdeII GATC 1 cut(s) 1123
NlaIII CATG 8 cut(s) 22, 284, 389, 508, 544, 737, 1102, 1264
NmuCI GTSAC 2 cut(s) 868, 1273
NspI RCATGY 3 cut(s) 22, 389, 508
PaeI GCATGC 1 cut(s) 389
PdmI GAANNNNTTC 1 cut(s) 744
PfeI GAWTC 4 cut(s) 332, 345, 365, 988
PkrI GCNGC 8 cut(s) 12, 17, 90, 129, 138, 155, 886, 913
PleI GAGTC 5 cut(s) 315, 631, 659, 735, 976
PpsI GAGTC 5 cut(s) 315, 631, 659, 735, 976
PshBI ATTAAT 1 cut(s) 1089
Psp6I CCWGG 3 cut(s) 139, 471, 1269
PspFI CCCAGC 1 cut(s) 1168
PspGI CCWGG 3 cut(s) 139, 471, 1269
PstI CTGCAG 1 cut(s) 877
RsaI GTAC 2 cut(s) 590, 1103
RsaNI GTAC 2 cut(s) 589, 1102
RseI CAYNNNNRTG 1 cut(s) 932
SaqAI TTAA 8 cut(s) 489, 650, 771, 984, 1038, 1056, 1080, 1089
SatI GCNGC 8 cut(s) 11, 16, 89, 128, 137, 154, 885, 912
Sau3AI GATC 1 cut(s) 1123
ScaI AGTACT 1 cut(s) 590
SchI GAGTC 5 cut(s) 315, 632, 659, 736, 977
ScrFI CCNGG 3 cut(s) 141, 473, 1271
SfcI CTRYAG 1 cut(s) 873
SmiMI CAYNNNNRTG 1 cut(s) 932
SmlI CTYRAG 3 cut(s) 305, 401, 659
SmoI CTYRAG 3 cut(s) 305, 401, 659
SphI GCATGC 1 cut(s) 389
Sse9I AATT 7 cut(s) 751, 864, 906, 1029, 1043, 1090, 1133
SsiI CCGC 8 cut(s) 16, 30, 103, 128, 166, 184, 641, 994
SspMI CTAG 2 cut(s) 93, 1026
StyD4I CCNGG 3 cut(s) 139, 471, 1269
TaaI ACNGT 2 cut(s) 577, 588
TaiI ACGT 2 cut(s) 816, 1212
TaqI TCGA 2 cut(s) 326, 1064
TasI AATT 7 cut(s) 751, 864, 906, 1029, 1043, 1090, 1133
TatI WGTACW 2 cut(s) 588, 1101
TauI GCSGC 2 cut(s) 18, 130
TfiI GAWTC 4 cut(s) 332, 345, 365, 988
Tru1I TTAA 8 cut(s) 489, 650, 771, 984, 1038, 1056, 1080, 1089
Tru9I TTAA 8 cut(s) 489, 650, 771, 984, 1038, 1056, 1080, 1089
TseFI GTSAC 2 cut(s) 868, 1273
TseI GCWGC 6 cut(s) 10, 88, 136, 153, 884, 911
Tsp45I GTSAC 2 cut(s) 868, 1273
TspDTI ATGAA 6 cut(s) 468, 552, 737, 750, 1163, 1244
VspI ATTAAT 1 cut(s) 1089
XagI CCTNNNNNAGG 1 cut(s) 897
XceI RCATGY 3 cut(s) 22, 389, 508
XcmI CCANNNNNNNNNTGG 1 cut(s) 1042
XmnI GAANNNNTTC 1 cut(s) 744
XspI CTAG 2 cut(s) 93, 1026
ZrmI AGTACT 1 cut(s) 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.