Rh6BG143200

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0008837
Physical Location & Seq
Reverse (-)
22938733 .. 22940078
1346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG143200.1

Sequence Viewer

Length: 828 bp
ATGCTGGATTTCCTTTCTGAAATAGAAAACTTCTTCCTGCTTGCTCGTAATAGCCATTCATATACTGCAAAAGTGATTCAACAGTACTTTGAGATACCCAATGGCATGAGTGGAGCTGAGTTCTACAAGAAGATTGAGGATAACTTAAAAAAGCTCAGGGGCTCTGATAATATCTTTGACCATTCTGCTGAAAACTTGCTCAAAAAGGCAGAAAGTTTGAAGAGTCAGCATGAAGAAATGCTTCGTAATTTGCAAGCAGAAATCTCTAAACTTACAAAGAAGGAGAAACGCGCGAAATTATGGAAGAACATCGCGTATATGATATTTGTAGGCGCTGCGGTTGGTTTGGCTATTGCCGGAATTGTGACTGCAGTTATAGCTGCTCCACATGTTGCAGCGGCTATTACTTCTGCCATTGGCCCCATAGCAATGGCTGGACACTGGACTGTCTCTTTGTTTGAAGAATCTGAAAGAGCATTTAAGAATCAGCGGGAATTAAATGATGAGAGGCGTAAGGAAACTAGAATTGCCATTAAAGAGTTGGGCAACATTAAGTCTTCGATTGATGGTGTTATTAACCAGATTAATTCAGTCATGTACAAGCCAGGCTCTGAAATGGATCGGGAACAATTAGATTTCACAATGGAGCAAATCAAGAGCAAGCTGGGAGAGTTTCTGAATAAGGTTGAGGATTTGGAGAAGGACGTAGAAAAGACCATAAAGTATATAAGGAAGGCAAGGGATGATGTTCATATCATGAACACCCAGGTCACGCGGGTCAAAAAAGGAGCACTTTGCTTTGAAAGCTTAGCTCGATCTGACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

275

Amino Acids

31.2

Weight (kDa)

8.72

Isoelectric Point (pI)

36.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 2 - 249 5.7e-33 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 291, 293, 314, 775
AciI CCGC 4 cut(s) 338, 398, 490, 775
AclWI GGATC 1 cut(s) 627
AfaI GTAC 2 cut(s) 86, 599
AflIII ACRYGT 1 cut(s) 388
AgsI TTSAA 4 cut(s) 80, 220, 461, 803
AjnI CCWGG 2 cut(s) 604, 765
AluBI AGCT 6 cut(s) 116, 154, 380, 664, 807, 812
AluI AGCT 6 cut(s) 116, 154, 380, 664, 807, 812
Alw21I GWGCWC 1 cut(s) 793
Alw26I GTCTC 1 cut(s) 454
AlwI GGATC 1 cut(s) 627
AlwNI CAGNNNCTG 1 cut(s) 611
AoxI GGCC 1 cut(s) 418
ApeKI GCWGC 3 cut(s) 335, 380, 395
ArsI GACNNNNNNTTYG 2 cut(s) 436, 468
AseI ATTAAT 1 cut(s) 585
Asp700I GAANNNNTTC 1 cut(s) 240
AspLEI GCGC 2 cut(s) 293, 335
AspS9I GGNCC 1 cut(s) 419
BanII GRGCYC 1 cut(s) 164
BbsI GAAGAC 1 cut(s) 549
Bbv12I GWGCWC 1 cut(s) 793
BbvI GCAGC 3 cut(s) 322, 367, 407
BccI CCATC 1 cut(s) 560
BciT130I CCWGG 2 cut(s) 606, 767
BcoDI GTCTC 1 cut(s) 454
BfaI CTAG 1 cut(s) 522
BfmI CTRYAG 1 cut(s) 369
BfoI RGCGCY 1 cut(s) 336
BisI GCNGC 4 cut(s) 336, 381, 396, 399
BlpI GCTNAGC 1 cut(s) 808
BlsI GCNGC 4 cut(s) 337, 382, 397, 400
BmcAI AGTACT 1 cut(s) 86
Bme1390I CCNGG 2 cut(s) 606, 767
BmgT120I GGNCC 1 cut(s) 419
BmiI GGNNCC 1 cut(s) 421
BmrFI CCNGG 2 cut(s) 606, 767
BpiI GAAGAC 1 cut(s) 549
Bpu10I CCTNAGC 1 cut(s) 155
Bpu1102I GCTNAGC 1 cut(s) 808
BsaJI CCNNGG 1 cut(s) 765
Bse1I ACTGG 1 cut(s) 446
Bse3DI GCAATG 1 cut(s) 435
BseBI CCWGG 2 cut(s) 606, 767
BseDI CCNNGG 1 cut(s) 765
BseGI GGATG 1 cut(s) 748
BseMI GCAATG 1 cut(s) 435
BseMII CTCAG 2 cut(s) 108, 169
BseNI ACTGG 1 cut(s) 446
BseXI GCAGC 3 cut(s) 322, 367, 407
BseYI CCCAGC 1 cut(s) 664
Bsh1236I CGCG 4 cut(s) 291, 293, 314, 775
BshFI GGCC 1 cut(s) 420
BsiHKAI GWGCWC 1 cut(s) 793
BsiSI CCGG 1 cut(s) 357
BsmAI GTCTC 1 cut(s) 454
BsnI GGCC 1 cut(s) 420
Bsp1286I GDGCHC 2 cut(s) 164, 793
Bsp1407I TGTACA 1 cut(s) 597
Bsp143I GATC 2 cut(s) 619, 815
Bsp1720I GCTNAGC 1 cut(s) 808
BspACI CCGC 4 cut(s) 338, 398, 490, 775
BspANI GGCC 1 cut(s) 420
BspCNI CTCAG 2 cut(s) 109, 168
BspFNI CGCG 4 cut(s) 291, 293, 314, 775
BspHI TCATGA 1 cut(s) 756
BspLI GGNNCC 1 cut(s) 421
BspMAI CTGCAG 1 cut(s) 373
BspPI GGATC 1 cut(s) 627
BsrDI GCAATG 1 cut(s) 435
BsrGI TGTACA 1 cut(s) 597
BsrI ACTGG 1 cut(s) 446
BssECI CCNNGG 1 cut(s) 765
BssMI GATC 2 cut(s) 619, 815
Bst2UI CCWGG 2 cut(s) 606, 767
Bst4CI ACNGT 2 cut(s) 84, 448
Bst6I CTCTTC 1 cut(s) 215
BstAUI TGTACA 1 cut(s) 597
BstC8I GCNNGC 3 cut(s) 42, 255, 662
BstDEI CTNAG 3 cut(s) 117, 155, 808
BstF5I GGATG 1 cut(s) 748
BstFNI CGCG 4 cut(s) 291, 293, 314, 775
BstH2I RGCGCY 1 cut(s) 336
BstHHI GCGC 2 cut(s) 293, 335
BstKTI GATC 2 cut(s) 622, 818
BstMAI GTCTC 1 cut(s) 454
BstMBI GATC 2 cut(s) 619, 815
BstMWI GCNNNNNNNGC 2 cut(s) 377, 804
BstNI CCWGG 2 cut(s) 606, 767
BstNSI RCATGY 1 cut(s) 392
BstSCI CCNGG 2 cut(s) 604, 765
BstSFI CTRYAG 1 cut(s) 369
BstUI CGCG 4 cut(s) 291, 293, 314, 775
BstV1I GCAGC 3 cut(s) 322, 367, 407
BstV2I GAAGAC 1 cut(s) 549
BstXI CCANNNNNNTGG 1 cut(s) 430
BsuRI GGCC 1 cut(s) 420
BtgZI GCGATG 1 cut(s) 295
BtsCI GGATG 1 cut(s) 748
BtsIMutI CAGTG 1 cut(s) 439
Cac8I GCNNGC 3 cut(s) 42, 255, 662
CaiI CAGNNNCTG 1 cut(s) 611
CciI TCATGA 1 cut(s) 756
CfoI GCGC 2 cut(s) 293, 335
Cfr13I GGNCC 1 cut(s) 419
Csp6I GTAC 2 cut(s) 85, 598
CviAII CATG 5 cut(s) 106, 230, 389, 595, 757
CviQI GTAC 2 cut(s) 85, 598
DdeI CTNAG 3 cut(s) 117, 155, 808
DpnI GATC 2 cut(s) 621, 817
DpnII GATC 2 cut(s) 619, 815
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
Eco24I GRGCYC 1 cut(s) 164
EcoRII CCWGG 2 cut(s) 604, 765
EcoT38I GRGCYC 1 cut(s) 164
FaeI CATG 5 cut(s) 109, 233, 392, 598, 760
FalI AAGNNNNNCTT 4 cut(s) 225, 257, 777, 809
FatI CATG 5 cut(s) 105, 229, 388, 594, 756
FauI CCCGC 2 cut(s) 483, 768
Fnu4HI GCNGC 4 cut(s) 336, 381, 396, 399
FokI GGATG 1 cut(s) 755
FriOI GRGCYC 1 cut(s) 164
Fsp4HI GCNGC 4 cut(s) 336, 381, 396, 399
FspBI CTAG 1 cut(s) 522
GlaI GCGC 2 cut(s) 292, 334
GluI GCNGC 4 cut(s) 336, 381, 396, 399
GsaI CCCAGC 1 cut(s) 668
HaeII RGCGCY 1 cut(s) 336
HaeIII GGCC 1 cut(s) 420
HapII CCGG 1 cut(s) 357
HhaI GCGC 2 cut(s) 293, 335
Hin1II CATG 5 cut(s) 109, 233, 392, 598, 760
Hin6I GCGC 2 cut(s) 291, 333
HinP1I GCGC 2 cut(s) 291, 333
HindIII AAGCTT 1 cut(s) 805
HinfI GANTC 4 cut(s) 76, 223, 464, 484
HpaII CCGG 1 cut(s) 357
Hpy188I TCNGA 6 cut(s) 19, 166, 469, 613, 678, 820
Hpy188III TCNNGA 3 cut(s) 623, 655, 757
HpyAV CCTTC 3 cut(s) 274, 694, 727
HpyCH4III ACNGT 2 cut(s) 84, 448
HpyCH4IV ACGT 1 cut(s) 705
HpyCH4V TGCA 4 cut(s) 68, 253, 371, 395
HpyF10VI GCNNNNNNNGC 2 cut(s) 377, 804
HpyF3I CTNAG 3 cut(s) 117, 155, 808
HpySE526I ACGT 1 cut(s) 705
Hsp92II CATG 5 cut(s) 109, 233, 392, 598, 760
HspAI GCGC 2 cut(s) 291, 333
Kzo9I GATC 2 cut(s) 619, 815
LmnI GCTCC 4 cut(s) 113, 388, 646, 788
Lsp1109I GCAGC 3 cut(s) 322, 367, 407
MaeI CTAG 1 cut(s) 522
MaeII ACGT 1 cut(s) 705
MaeIII GTNAC 2 cut(s) 364, 769
MalI GATC 2 cut(s) 621, 817
MboI GATC 2 cut(s) 619, 815
MboII GAAGA 7 cut(s) 25, 142, 232, 245, 316, 473, 549
MhlI GDGCHC 2 cut(s) 164, 793
MluCI AATT 7 cut(s) 247, 296, 360, 494, 525, 586, 629
MlyI GAGTC 1 cut(s) 232
MnlI CCTC 3 cut(s) 130, 501, 682
MroXI GAANNNNTTC 1 cut(s) 240
MseI TTAA 7 cut(s) 146, 480, 497, 534, 552, 576, 585
MslI CAYNNNNRTG 1 cut(s) 428
MspA1I CMGCKG 2 cut(s) 398, 490
MspI CCGG 1 cut(s) 357
MspR9I CCNGG 2 cut(s) 606, 767
MvaI CCWGG 2 cut(s) 606, 767
MvnI CGCG 4 cut(s) 291, 293, 314, 775
MwoI GCNNNNNNNGC 2 cut(s) 377, 804
NdeII GATC 2 cut(s) 619, 815
NlaIII CATG 5 cut(s) 109, 233, 392, 598, 760
NlaIV GGNNCC 1 cut(s) 421
NmuCI GTSAC 2 cut(s) 364, 769
NspI RCATGY 1 cut(s) 392
PagI TCATGA 1 cut(s) 756
PciI ACATGT 1 cut(s) 388
PdmI GAANNNNTTC 1 cut(s) 240
PfeI GAWTC 3 cut(s) 76, 464, 484
PkrI GCNGC 4 cut(s) 337, 382, 397, 400
PleI GAGTC 1 cut(s) 231
PpsI GAGTC 1 cut(s) 231
PscI ACATGT 1 cut(s) 388
PshBI ATTAAT 1 cut(s) 585
Psp6I CCWGG 2 cut(s) 604, 765
PspFI CCCAGC 1 cut(s) 664
PspGI CCWGG 2 cut(s) 604, 765
PspN4I GGNNCC 1 cut(s) 421
PspPI GGNCC 1 cut(s) 419
PstI CTGCAG 1 cut(s) 373
PstNI CAGNNNCTG 1 cut(s) 611
RsaI GTAC 2 cut(s) 86, 599
RsaNI GTAC 2 cut(s) 85, 598
RseI CAYNNNNRTG 1 cut(s) 428
SaqAI TTAA 7 cut(s) 146, 480, 497, 534, 552, 576, 585
SatI GCNGC 4 cut(s) 336, 381, 396, 399
Sau3AI GATC 2 cut(s) 619, 815
Sau96I GGNCC 1 cut(s) 419
ScaI AGTACT 1 cut(s) 86
SchI GAGTC 1 cut(s) 232
ScrFI CCNGG 2 cut(s) 606, 767
SduI GDGCHC 2 cut(s) 164, 793
SetI ASST 9 cut(s) 118, 156, 382, 666, 687, 708, 771, 809, 814
SfcI CTRYAG 1 cut(s) 369
SmiMI CAYNNNNRTG 1 cut(s) 428
Sse9I AATT 7 cut(s) 247, 296, 360, 494, 525, 586, 629
SsiI CCGC 4 cut(s) 338, 398, 490, 775
SspMI CTAG 1 cut(s) 522
StyD4I CCNGG 2 cut(s) 604, 765
TaaI ACNGT 2 cut(s) 84, 448
TaiI ACGT 1 cut(s) 708
TaqI TCGA 2 cut(s) 560, 814
TasI AATT 7 cut(s) 247, 296, 360, 494, 525, 586, 629
TatI WGTACW 2 cut(s) 84, 597
TauI GCSGC 1 cut(s) 401
TfiI GAWTC 3 cut(s) 76, 464, 484
Tru1I TTAA 7 cut(s) 146, 480, 497, 534, 552, 576, 585
Tru9I TTAA 7 cut(s) 146, 480, 497, 534, 552, 576, 585
TscAI CASTG 1 cut(s) 446
TseFI GTSAC 2 cut(s) 364, 769
TseI GCWGC 3 cut(s) 335, 380, 395
Tsp45I GTSAC 2 cut(s) 364, 769
TspDTI ATGAA 4 cut(s) 48, 246, 740, 773
TspRI CASTG 1 cut(s) 446
VspI ATTAAT 1 cut(s) 585
XceI RCATGY 1 cut(s) 392
XcmI CCANNNNNNNNNTGG 1 cut(s) 538
XmnI GAANNNNTTC 1 cut(s) 240
XspI CTAG 1 cut(s) 522
ZrmI AGTACT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.