Rh6CG112700

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0008837
Physical Location & Seq
Forward (+)
12956647 .. 12957228
582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG112700.1

Sequence Viewer

Length: 582 bp
ATGATTCATAATTTGCAAGTCGAAATCATTAAACTTAGTAAGAAGGAGAATCTCGCGAAATCATGGAAGAACGTCGCGTTTAATTATGATATTTGTAGGCGGTGTGGTTGGTTTGGCTATTGCAGAATTGTGACTGCAGTTATAGGTGCTCCACCTGTTGCAGAAGCAATTGCTGCTGGCATTGTCCCGATAGCAATGGCTGGACACTGGACTATCTCTTGGTTTGAAGAGTCAGAAAAAGCATTTAAGAATCAACGGGAAGTAAATGAGGCGGGGCGTAAGGAAACTAGAATTGCCGTTAAAAAGTTGGGCAACACTAAGTCTTCGATTGATGGTGTTATTAAACAGATTAATTCAGTCATGTACAATCCAGGCTCTCAAATGGATTGGGAACAATTAGATTTCACAATGAAGGAAATCAAGAGCAAGCTGGGAGCGTTTCTGAATAAGGTTGAGGATTTGGAGAAGGACGTTGAAAAGACCAAAAAGGATACAAGGAAGGCAAGGGATGATGTTCATATCATGGACACCCAGGTCACACAGGTCCAAAAAAGGAGCATTTTGTTTTCAAAACTAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

193

Amino Acids

21.96

Weight (kDa)

9.36

Isoelectric Point (pI)

29.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 41 - 171 4.7e-16 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 533
AccII CGCG 2 cut(s) 56, 77
AciI CCGC 2 cut(s) 100, 272
AfaI GTAC 1 cut(s) 365
AgsI TTSAA 3 cut(s) 227, 476, 570
AjnI CCWGG 2 cut(s) 370, 531
AluBI AGCT 1 cut(s) 430
AluI AGCT 1 cut(s) 430
Alw21I GWGCWC 1 cut(s) 151
ApeKI GCWGC 1 cut(s) 173
AseI ATTAAT 1 cut(s) 351
AspS9I GGNCC 1 cut(s) 544
AvaII GGWCC 1 cut(s) 544
BbsI GAAGAC 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 151
BbvI GCAGC 1 cut(s) 160
BccI CCATC 1 cut(s) 326
BceAI ACGGC 1 cut(s) 281
BciT130I CCWGG 2 cut(s) 372, 533
BciVI GTATCC 1 cut(s) 484
BfaI CTAG 2 cut(s) 288, 575
BfmI CTRYAG 1 cut(s) 135
BfuI GTATCC 1 cut(s) 484
BisI GCNGC 1 cut(s) 174
BlsI GCNGC 1 cut(s) 175
Bme1390I CCNGG 2 cut(s) 372, 533
Bme18I GGWCC 1 cut(s) 544
BmgT120I GGNCC 1 cut(s) 544
BmrFI CCNGG 2 cut(s) 372, 533
BpiI GAAGAC 1 cut(s) 315
BsaJI CCNNGG 1 cut(s) 531
Bse1I ACTGG 1 cut(s) 212
Bse3DI GCAATG 1 cut(s) 201
BseBI CCWGG 2 cut(s) 372, 533
BseDI CCNNGG 1 cut(s) 531
BseGI GGATG 1 cut(s) 514
BseMI GCAATG 1 cut(s) 201
BseNI ACTGG 1 cut(s) 212
BseXI GCAGC 1 cut(s) 160
BseYI CCCAGC 1 cut(s) 430
Bsh1236I CGCG 2 cut(s) 56, 77
BsiHKAI GWGCWC 1 cut(s) 151
BslFI GGGAC 1 cut(s) 170
BsmFI GGGAC 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 151
Bsp1407I TGTACA 1 cut(s) 363
Bsp68I TCGCGA 1 cut(s) 56
BspACI CCGC 2 cut(s) 100, 272
BspFNI CGCG 2 cut(s) 56, 77
BspMAI CTGCAG 1 cut(s) 139
BsrDI GCAATG 1 cut(s) 201
BsrGI TGTACA 1 cut(s) 363
BsrI ACTGG 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 531
Bst2UI CCWGG 2 cut(s) 372, 533
Bst6I CTCTTC 1 cut(s) 222
BstAPI GCANNNNNTGC 1 cut(s) 173
BstAUI TGTACA 1 cut(s) 363
BstC8I GCNNGC 2 cut(s) 178, 428
BstDEI CTNAG 2 cut(s) 35, 318
BstF5I GGATG 1 cut(s) 514
BstFNI CGCG 2 cut(s) 56, 77
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstNI CCWGG 2 cut(s) 372, 533
BstSCI CCNGG 2 cut(s) 370, 531
BstSFI CTRYAG 1 cut(s) 135
BstUI CGCG 2 cut(s) 56, 77
BstV1I GCAGC 1 cut(s) 160
BstV2I GAAGAC 1 cut(s) 315
BsuI GTATCC 1 cut(s) 484
BtsCI GGATG 1 cut(s) 514
BtsIMutI CAGTG 1 cut(s) 205
BtuMI TCGCGA 1 cut(s) 56
Cac8I GCNNGC 2 cut(s) 178, 428
Cfr13I GGNCC 1 cut(s) 544
Csp6I GTAC 1 cut(s) 364
CviAII CATG 4 cut(s) 63, 361, 523, 579
CviJI RGCY 4 cut(s) 117, 200, 375, 430
CviKI_1 RGCY 4 cut(s) 117, 200, 375, 430
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 2 cut(s) 35, 318
DrdI GACNNNNNNGTC 1 cut(s) 533
DseDI GACNNNNNNGTC 1 cut(s) 533
Eam1104I CTCTTC 1 cut(s) 222
EarI CTCTTC 1 cut(s) 222
Eco47I GGWCC 1 cut(s) 544
EcoRII CCWGG 2 cut(s) 370, 531
FaeI CATG 4 cut(s) 66, 364, 526, 582
FaiI YATR 8 cut(s) 9, 64, 87, 143, 362, 519, 524, 580
FaqI GGGAC 1 cut(s) 170
FatI CATG 4 cut(s) 62, 360, 522, 578
FauI CCCGC 1 cut(s) 265
Fnu4HI GCNGC 1 cut(s) 174
FokI GGATG 1 cut(s) 521
Fsp4HI GCNGC 1 cut(s) 174
FspBI CTAG 2 cut(s) 288, 575
GluI GCNGC 1 cut(s) 174
GsaI CCCAGC 1 cut(s) 434
Hin1II CATG 4 cut(s) 66, 364, 526, 582
HinfI GANTC 4 cut(s) 4, 49, 230, 250
Hpy188I TCNGA 2 cut(s) 235, 444
Hpy188III TCNNGA 3 cut(s) 55, 187, 421
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 4 cut(s) 37, 406, 460, 493
HpyCH4IV ACGT 2 cut(s) 72, 471
HpyCH4V TGCA 4 cut(s) 16, 123, 137, 161
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
HpyF3I CTNAG 2 cut(s) 35, 318
HpySE526I ACGT 2 cut(s) 72, 471
Hsp92II CATG 4 cut(s) 66, 364, 526, 582
LmnI GCTCC 3 cut(s) 154, 434, 555
Lsp1109I GCAGC 1 cut(s) 160
MaeI CTAG 2 cut(s) 288, 575
MaeII ACGT 2 cut(s) 72, 471
MaeIII GTNAC 2 cut(s) 130, 535
MboII GAAGA 3 cut(s) 79, 239, 315
MfeI CAATTG 1 cut(s) 168
MhlI GDGCHC 1 cut(s) 151
MluCI AATT 7 cut(s) 10, 82, 126, 168, 291, 352, 395
MlyI GAGTC 1 cut(s) 239
MnlI CCTC 2 cut(s) 262, 448
MseI TTAA 6 cut(s) 30, 81, 246, 300, 342, 351
MspR9I CCNGG 2 cut(s) 372, 533
MunI CAATTG 1 cut(s) 168
MvaI CCWGG 2 cut(s) 372, 533
MvnI CGCG 2 cut(s) 56, 77
MwoI GCNNNNNNNGC 1 cut(s) 173
NlaIII CATG 4 cut(s) 66, 364, 526, 582
NmuCI GTSAC 2 cut(s) 130, 535
NruI TCGCGA 1 cut(s) 56
PfeI GAWTC 3 cut(s) 4, 49, 250
PkrI GCNGC 1 cut(s) 175
PleI GAGTC 1 cut(s) 238
PpsI GAGTC 1 cut(s) 238
PshBI ATTAAT 1 cut(s) 351
Psp6I CCWGG 2 cut(s) 370, 531
PspFI CCCAGC 1 cut(s) 430
PspGI CCWGG 2 cut(s) 370, 531
PspPI GGNCC 1 cut(s) 544
PstI CTGCAG 1 cut(s) 139
RruI TCGCGA 1 cut(s) 56
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
SaqAI TTAA 6 cut(s) 30, 81, 246, 300, 342, 351
SatI GCNGC 1 cut(s) 174
Sau96I GGNCC 1 cut(s) 544
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 2 cut(s) 372, 533
SduI GDGCHC 1 cut(s) 151
SetI ASST 8 cut(s) 75, 148, 157, 432, 453, 474, 537, 546
SfcI CTRYAG 1 cut(s) 135
SinI GGWCC 1 cut(s) 544
Sse9I AATT 7 cut(s) 10, 82, 126, 168, 291, 352, 395
SsiI CCGC 2 cut(s) 100, 272
SspMI CTAG 2 cut(s) 288, 575
StyD4I CCNGG 2 cut(s) 370, 531
TaiI ACGT 2 cut(s) 75, 474
TaqI TCGA 2 cut(s) 21, 326
TasI AATT 7 cut(s) 10, 82, 126, 168, 291, 352, 395
TatI WGTACW 1 cut(s) 363
TfiI GAWTC 3 cut(s) 4, 49, 250
Tru1I TTAA 6 cut(s) 30, 81, 246, 300, 342, 351
Tru9I TTAA 6 cut(s) 30, 81, 246, 300, 342, 351
TscAI CASTG 1 cut(s) 212
TseFI GTSAC 2 cut(s) 130, 535
TseI GCWGC 1 cut(s) 173
Tsp45I GTSAC 2 cut(s) 130, 535
TspDTI ATGAA 2 cut(s) 425, 506
TspRI CASTG 1 cut(s) 212
VpaK11BI GGWCC 1 cut(s) 544
VspI ATTAAT 1 cut(s) 351
XspI CTAG 2 cut(s) 288, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.