Rw6G012370

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0008837
Physical Location & Seq
Reverse (-)
22497572 .. 22498813
1242 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G012370.1

Sequence Viewer

Length: 1098 bp
ATGAAGACCCTTACCTGCTTTACAGCTGAGATTCCTACGAAATATTCCTATAGCGAGTTAGTCTCCACAGCTGAATGGAAGTCATTTCAGGAACATTGCAAGGAAAACGCAGACTTGCAATCGTGGGACACAGAACTCAAAGAACATGCAGAAGAGATCTTGAAAACCGTCAAGAGTGAAGGAGGTCTCTCGATTGATTCCTGGAATCAATCACACACTACTGCAATCTTGGCATTCAAGAAAGGTATAACTGAAGGGGAGGGGAGTAGTAGGAAGAAGAAGAAAAATGAAGAAAGAGGGAACATCCTGACTGACGAGTATATTAAGAATAGCTCAAACATGCTGGATTTACTTTCTGAAATGGAAAACTTCCTCCTGCTTGCTCGTAATAGCCATTCATATATTGCAAAAATGATTCAACAGTACTTTGAGATACCCAATGGCATGAGTGGAGCTGAGTTCTACGGGAAGATTGAGGATAACTTAAAAAAGCTCAGGGGCTTTGATAATATCTTTGACCATTCTGCTGAAAACTTGCTCAAAAAGGCAGAAAGTTTGAAGAGTCAGTATGAAGAAATGCTTAGTAATTTGCAAGCCGAAATCTCTAAACTTGTTAAGAAGGAGAAACGCGCAAAATTATGGAAGAATGTCGCGTATATGATATTTGTAGGCGCTGCGGTTGGTTTGGCTATTGCCGGAATTGTGACTGTAGTTATAGCTGCTCCACCTGTTGCAGCAGCTATTACTTCTGCCATTGGCCCCATAGCAATGGCTGGAAACTGGACTGTCTCTTTGCTTGAAGAGTCTGAAAGAGCATTTAAGAATCAGCTGGAATTAAATGAGAAGATGCGTAAGGAAACTAGAATTTCCATTAAAGACTTGGGCAACATTAAGTCTTCGATTGATGGTGTTATTAACCAGATTAATTCAGTCATGTACAATCCAGGCTCTGAAATGGATCGGGAACAATTAGATTTCACAATGAAGGAAATCAAGAGCAAGCTGGGAGCGTTTCTGAATAAGGTTGAGGATTTGGAGAAGGACGTAGAAAAGACCATAAAGTATATAAGGAAGGCAAGGGATGACGTCATATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

365

Amino Acids

41.15

Weight (kDa)

6.1

Isoelectric Point (pI)

35.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 39 - 363 1.1e-38 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1089
Acc36I ACCTGC 1 cut(s) 23
AccII CGCG 2 cut(s) 630, 653
AciI CCGC 1 cut(s) 677
AclWI GGATC 1 cut(s) 966
AcsI RAATTY 1 cut(s) 864
AcuI CTGAAG 1 cut(s) 273
AcyI GRCGYC 1 cut(s) 1086
AfaI GTAC 2 cut(s) 425, 938
AgsI TTSAA 5 cut(s) 163, 238, 419, 559, 800
AjnI CCWGG 2 cut(s) 200, 943
AluBI AGCT 9 cut(s) 26, 71, 333, 455, 493, 719, 740, 829, 1003
AluI AGCT 9 cut(s) 26, 71, 333, 455, 493, 719, 740, 829, 1003
Alw26I GTCTC 3 cut(s) 67, 191, 793
AlwI GGATC 1 cut(s) 966
AlwNI CAGNNNCTG 1 cut(s) 950
AoxI GGCC 1 cut(s) 757
ApeKI GCWGC 4 cut(s) 674, 719, 734, 737
ApoI RAATTY 1 cut(s) 864
ArsI GACNNNNNNTTYG 2 cut(s) 775, 807
AseI ATTAAT 1 cut(s) 924
AspLEI GCGC 2 cut(s) 632, 674
AspS9I GGNCC 1 cut(s) 758
BbsI GAAGAC 2 cut(s) 11, 888
BbvI GCAGC 4 cut(s) 661, 706, 746, 749
BccI CCATC 1 cut(s) 899
BciT130I CCWGG 2 cut(s) 202, 945
BcoDI GTCTC 3 cut(s) 67, 191, 793
BfaI CTAG 1 cut(s) 861
BfmI CTRYAG 2 cut(s) 49, 708
BfoI RGCGCY 1 cut(s) 675
BfuAI ACCTGC 1 cut(s) 23
BglII AGATCT 1 cut(s) 156
BisI GCNGC 4 cut(s) 675, 720, 735, 738
BlsI GCNGC 4 cut(s) 676, 721, 736, 739
BmcAI AGTACT 1 cut(s) 425
Bme1390I CCNGG 2 cut(s) 202, 945
BmgT120I GGNCC 1 cut(s) 758
BmiI GGNNCC 1 cut(s) 760
BmrFI CCNGG 2 cut(s) 202, 945
BmsI GCATC 1 cut(s) 837
BpiI GAAGAC 2 cut(s) 11, 888
BplI GAGNNNNNCTC 2 cut(s) 47, 79
Bpu10I CCTNAGC 1 cut(s) 494
BsaHI GRCGYC 1 cut(s) 1086
BsaI GGTCTC 1 cut(s) 191
Bse1I ACTGG 1 cut(s) 785
Bse3DI GCAATG 2 cut(s) 94, 774
BseBI CCWGG 2 cut(s) 202, 945
BseGI GGATG 2 cut(s) 303, 1087
BseMI GCAATG 2 cut(s) 94, 774
BseMII CTCAG 3 cut(s) 18, 447, 508
BseNI ACTGG 1 cut(s) 785
BseXI GCAGC 4 cut(s) 661, 706, 746, 749
BseYI CCCAGC 1 cut(s) 1003
Bsh1236I CGCG 2 cut(s) 630, 653
BshFI GGCC 1 cut(s) 759
BsiSI CCGG 1 cut(s) 696
BslFI GGGAC 1 cut(s) 140
BsmAI GTCTC 3 cut(s) 67, 191, 793
BsmFI GGGAC 1 cut(s) 140
BsmI GAATGC 1 cut(s) 233
BsnI GGCC 1 cut(s) 759
Bso31I GGTCTC 1 cut(s) 191
Bsp1407I TGTACA 1 cut(s) 936
Bsp143I GATC 2 cut(s) 156, 958
BspACI CCGC 1 cut(s) 677
BspANI GGCC 1 cut(s) 759
BspCNI CTCAG 3 cut(s) 19, 448, 507
BspFNI CGCG 2 cut(s) 630, 653
BspHI TCATGA 1 cut(s) 1094
BspLI GGNNCC 1 cut(s) 760
BspMI ACCTGC 1 cut(s) 23
BspPI GGATC 1 cut(s) 966
BspTNI GGTCTC 1 cut(s) 191
BsrDI GCAATG 2 cut(s) 94, 774
BsrGI TGTACA 1 cut(s) 936
BsrI ACTGG 1 cut(s) 785
BssMI GATC 2 cut(s) 156, 958
BssNI GRCGYC 1 cut(s) 1086
Bst2UI CCWGG 2 cut(s) 202, 945
Bst4CI ACNGT 4 cut(s) 169, 423, 709, 787
Bst6I CTCTTC 3 cut(s) 147, 554, 795
BstACI GRCGYC 1 cut(s) 1086
BstAUI TGTACA 1 cut(s) 936
BstC8I GCNNGC 3 cut(s) 381, 594, 1001
BstDEI CTNAG 4 cut(s) 27, 456, 494, 581
BstF5I GGATG 2 cut(s) 303, 1087
BstFNI CGCG 2 cut(s) 630, 653
BstH2I RGCGCY 1 cut(s) 675
BstHHI GCGC 2 cut(s) 632, 674
BstKTI GATC 2 cut(s) 159, 961
BstMAI GTCTC 3 cut(s) 67, 191, 793
BstMBI GATC 2 cut(s) 156, 958
BstMWI GCNNNNNNNGC 1 cut(s) 230
BstNI CCWGG 2 cut(s) 202, 945
BstNSI RCATGY 2 cut(s) 149, 343
BstSCI CCNGG 2 cut(s) 200, 943
BstSFI CTRYAG 2 cut(s) 49, 708
BstUI CGCG 2 cut(s) 630, 653
BstV1I GCAGC 4 cut(s) 661, 706, 746, 749
BstV2I GAAGAC 2 cut(s) 11, 888
BstX2I RGATCY 1 cut(s) 156
BstXI CCANNNNNNTGG 1 cut(s) 769
BstYI RGATCY 1 cut(s) 156
BsuRI GGCC 1 cut(s) 759
BtsCI GGATG 2 cut(s) 303, 1087
BveI ACCTGC 1 cut(s) 23
Cac8I GCNNGC 3 cut(s) 381, 594, 1001
CaiI CAGNNNCTG 1 cut(s) 950
CciI TCATGA 1 cut(s) 1094
CfoI GCGC 2 cut(s) 632, 674
Cfr13I GGNCC 1 cut(s) 758
Csp6I GTAC 2 cut(s) 424, 937
CviAII CATG 5 cut(s) 146, 340, 445, 934, 1095
CviQI GTAC 2 cut(s) 424, 937
DdeI CTNAG 4 cut(s) 27, 456, 494, 581
DpnI GATC 2 cut(s) 158, 960
DpnII GATC 2 cut(s) 156, 958
Eam1104I CTCTTC 3 cut(s) 147, 554, 795
EarI CTCTTC 3 cut(s) 147, 554, 795
Eco31I GGTCTC 1 cut(s) 191
Eco57I CTGAAG 1 cut(s) 273
EcoRII CCWGG 2 cut(s) 200, 943
FaeI CATG 5 cut(s) 149, 343, 448, 937, 1098
FalI AAGNNNNNCTT 2 cut(s) 564, 596
FaqI GGGAC 1 cut(s) 140
FatI CATG 5 cut(s) 145, 339, 444, 933, 1094
Fnu4HI GCNGC 4 cut(s) 675, 720, 735, 738
FokI GGATG 2 cut(s) 290, 1094
Fsp4HI GCNGC 4 cut(s) 675, 720, 735, 738
FspBI CTAG 1 cut(s) 861
GlaI GCGC 2 cut(s) 631, 673
GluI GCNGC 4 cut(s) 675, 720, 735, 738
GsaI CCCAGC 1 cut(s) 1007
HaeII RGCGCY 1 cut(s) 675
HaeIII GGCC 1 cut(s) 759
HapII CCGG 1 cut(s) 696
HhaI GCGC 2 cut(s) 632, 674
Hin1I GRCGYC 1 cut(s) 1086
Hin1II CATG 5 cut(s) 149, 343, 448, 937, 1098
Hin6I GCGC 2 cut(s) 630, 672
HinP1I GCGC 2 cut(s) 630, 672
HinfI GANTC 7 cut(s) 31, 197, 205, 415, 562, 803, 823
HpaII CCGG 1 cut(s) 696
Hpy188I TCNGA 4 cut(s) 358, 808, 952, 1017
Hpy188III TCNNGA 9 cut(s) 89, 160, 172, 190, 238, 307, 962, 994, 1095
HpyAV CCTTC 6 cut(s) 173, 248, 613, 979, 1033, 1066
HpyCH4III ACNGT 4 cut(s) 169, 423, 709, 787
HpyCH4IV ACGT 2 cut(s) 1044, 1086
HpyCH4V TGCA 7 cut(s) 99, 118, 149, 224, 407, 592, 734
HpyF10VI GCNNNNNNNGC 1 cut(s) 230
HpyF3I CTNAG 4 cut(s) 27, 456, 494, 581
HpySE526I ACGT 2 cut(s) 1044, 1086
Hsp92I GRCGYC 1 cut(s) 1086
Hsp92II CATG 5 cut(s) 149, 343, 448, 937, 1098
HspAI GCGC 2 cut(s) 630, 672
Kzo9I GATC 2 cut(s) 156, 958
LmnI GCTCC 3 cut(s) 452, 727, 1007
Lsp1109I GCAGC 4 cut(s) 661, 706, 746, 749
LweI GCATC 1 cut(s) 837
MaeI CTAG 1 cut(s) 861
MaeII ACGT 2 cut(s) 1044, 1086
MaeIII GTNAC 1 cut(s) 703
MalI GATC 2 cut(s) 158, 960
MboI GATC 2 cut(s) 156, 958
MflI RGATCY 1 cut(s) 156
MluCI AATT 7 cut(s) 586, 635, 699, 833, 864, 925, 968
MlyI GAGTC 2 cut(s) 571, 812
MnlI CCTC 6 cut(s) 176, 253, 290, 383, 469, 1021
MseI TTAA 9 cut(s) 324, 485, 615, 819, 836, 873, 891, 915, 924
MslI CAYNNNNRTG 1 cut(s) 767
MspA1I CMGCKG 3 cut(s) 26, 71, 829
MspI CCGG 1 cut(s) 696
MspR9I CCNGG 2 cut(s) 202, 945
Mva1269I GAATGC 1 cut(s) 233
MvaI CCWGG 2 cut(s) 202, 945
MvnI CGCG 2 cut(s) 630, 653
MwoI GCNNNNNNNGC 1 cut(s) 230
NdeII GATC 2 cut(s) 156, 958
NlaIII CATG 5 cut(s) 149, 343, 448, 937, 1098
NlaIV GGNNCC 1 cut(s) 760
NmuCI GTSAC 1 cut(s) 703
NspI RCATGY 2 cut(s) 149, 343
PagI TCATGA 1 cut(s) 1094
PctI GAATGC 1 cut(s) 233
PfeI GAWTC 5 cut(s) 31, 197, 205, 415, 823
PfoI TCCNGGA 1 cut(s) 200
PkrI GCNGC 4 cut(s) 676, 721, 736, 739
PleI GAGTC 2 cut(s) 570, 811
PpsI GAGTC 2 cut(s) 570, 811
PshBI ATTAAT 1 cut(s) 924
Psp6I CCWGG 2 cut(s) 200, 943
PspFI CCCAGC 1 cut(s) 1003
PspGI CCWGG 2 cut(s) 200, 943
PspN4I GGNNCC 1 cut(s) 760
PspPI GGNCC 1 cut(s) 758
PstNI CAGNNNCTG 1 cut(s) 950
PsuI RGATCY 1 cut(s) 156
PvuII CAGCTG 3 cut(s) 26, 71, 829
RsaI GTAC 2 cut(s) 425, 938
RsaNI GTAC 2 cut(s) 424, 937
RseI CAYNNNNRTG 1 cut(s) 767
SaqAI TTAA 9 cut(s) 324, 485, 615, 819, 836, 873, 891, 915, 924
SatI GCNGC 4 cut(s) 675, 720, 735, 738
Sau3AI GATC 2 cut(s) 156, 958
Sau96I GGNCC 1 cut(s) 758
ScaI AGTACT 1 cut(s) 425
SchI GAGTC 2 cut(s) 571, 812
ScrFI CCNGG 2 cut(s) 202, 945
SfaNI GCATC 1 cut(s) 837
SfcI CTRYAG 2 cut(s) 49, 708
SmiMI CAYNNNNRTG 1 cut(s) 767
Sse9I AATT 7 cut(s) 586, 635, 699, 833, 864, 925, 968
SsiI CCGC 1 cut(s) 677
SspI AATATT 1 cut(s) 44
SspMI CTAG 1 cut(s) 861
StyD4I CCNGG 2 cut(s) 200, 943
TaaI ACNGT 4 cut(s) 169, 423, 709, 787
TaiI ACGT 2 cut(s) 1047, 1089
TaqI TCGA 2 cut(s) 191, 899
TasI AATT 7 cut(s) 586, 635, 699, 833, 864, 925, 968
TatI WGTACW 2 cut(s) 423, 936
TfiI GAWTC 5 cut(s) 31, 197, 205, 415, 823
Tru1I TTAA 9 cut(s) 324, 485, 615, 819, 836, 873, 891, 915, 924
Tru9I TTAA 9 cut(s) 324, 485, 615, 819, 836, 873, 891, 915, 924
TseFI GTSAC 1 cut(s) 703
TseI GCWGC 4 cut(s) 674, 719, 734, 737
Tsp45I GTSAC 1 cut(s) 703
TspDTI ATGAA 5 cut(s) 17, 303, 387, 585, 998
VspI ATTAAT 1 cut(s) 924
XapI RAATTY 1 cut(s) 864
XceI RCATGY 2 cut(s) 149, 343
XcmI CCANNNNNNNNNTGG 1 cut(s) 877
XspI CTAG 1 cut(s) 861
ZraI GACGTC 1 cut(s) 1087
ZrmI AGTACT 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.