FvH4_2g08240

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007487
Physical Location & Seq
Reverse (-)
7161781 .. 7165851
4071 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g08240.t1

Sequence Viewer

Length: 642 bp
ATGGCATCTAATGTCAATGACCACTCTGGTGAAAAGTTGGTCAAAAAGGCTCGTTCTTTGCTTGAGAGGCAGGAAGAAATGTTCTCCAAATGTCTCCTTGAGCAAAAGAAGCTTGGGAAGGACCAGAAGAGTATTCATGGTTGGAGAAAGCTGTTGAATTATATATCCATGGGGACTAAGTGCCTCGTTCTCATTGTTTTGGTTGGGATGATGGCCAATTCTATAGGATCTGTGAGAGCTGGTTCGTCTAATGTTGTTGCAGATTTGTCGATCAAAGCAGCCTGCATGATTGATATCTCGGTTTTTATAGAGAAGGCAGATCACTGGAATAGCTCCCACCTCGAAGCATGTGACAATGAGCTTGTGCTGAAGAATCATGCAATGAACACAATGCAGAAAGGGGCTTTACATTGCATAAAAGAGATGGGGGAAATTAAAGATCTCATTGGTAAGGTTGTAAATTCTATTGAGTCTCTTCTTGCTTTAAGTACAAACGTGGCGAATGGTGGAAATATAGAAGTCGCAATCACAAATATCAAGTGTTTCCTGAATAAGTTTTTGGAAAAAATTGAGGTTTTGGAGAAGAAAACAGGGGAATGCAGCTCTAAAATTTTGAAAGCAAGGCAAGCTGTTCTTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

214

Amino Acids

23.48

Weight (kDa)

9.03

Isoelectric Point (pI)

36.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 6 - 212 1.2e-18 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 235
AcoI YGGCCR 1 cut(s) 213
AcsI RAATTY 2 cut(s) 460, 609
AcuI CTGAAG 1 cut(s) 389
AfaI GTAC 1 cut(s) 490
AgsI TTSAA 2 cut(s) 157, 616
AjuI GAANNNNNNNTTGG 2 cut(s) 542, 574
AleI CACNNNNGTG 1 cut(s) 27
AloI GAACNNNNNNTCC 2 cut(s) 65, 97
AluBI AGCT 7 cut(s) 112, 151, 239, 333, 361, 603, 629
AluI AGCT 7 cut(s) 112, 151, 239, 333, 361, 603, 629
Alw26I GTCTC 2 cut(s) 98, 477
AlwI GGATC 1 cut(s) 235
AoxI GGCC 1 cut(s) 213
ApeKI GCWGC 2 cut(s) 278, 600
ApoI RAATTY 2 cut(s) 460, 609
AspS9I GGNCC 1 cut(s) 121
AsuHPI GGTGA 1 cut(s) 41
AvaII GGWCC 1 cut(s) 121
BalI TGGCCA 1 cut(s) 215
BbvI GCAGC 2 cut(s) 290, 612
BccI CCATC 2 cut(s) 205, 418
BcgI CGANNNNNNTGC 2 cut(s) 249, 283
BcoDI GTCTC 2 cut(s) 98, 477
BfmI CTRYAG 1 cut(s) 222
BglII AGATCT 1 cut(s) 439
BisI GCNGC 2 cut(s) 279, 601
BlsI GCNGC 2 cut(s) 280, 602
Bme18I GGWCC 1 cut(s) 121
BmgT120I GGNCC 1 cut(s) 121
BmsI GCATC 1 cut(s) 14
BpuEI CTTGAG 2 cut(s) 83, 119
BsaBI GATNNNNATC 1 cut(s) 293
BsaJI CCNNGG 1 cut(s) 168
Bse1I ACTGG 1 cut(s) 329
Bse3DI GCAATG 2 cut(s) 387, 409
Bse8I GATNNNNATC 1 cut(s) 293
BseDI CCNNGG 1 cut(s) 168
BseGI GGATG 1 cut(s) 213
BseJI GATNNNNATC 1 cut(s) 293
BseMI GCAATG 2 cut(s) 387, 409
BseNI ACTGG 1 cut(s) 329
BseXI GCAGC 2 cut(s) 290, 612
BshFI GGCC 1 cut(s) 215
BslFI GGGAC 1 cut(s) 187
BsmAI GTCTC 2 cut(s) 98, 477
BsmFI GGGAC 1 cut(s) 187
BsmI GAATGC 1 cut(s) 602
BsnI GGCC 1 cut(s) 215
Bsp143I GATC 4 cut(s) 227, 270, 319, 439
Bsp19I CCATGG 1 cut(s) 168
BspANI GGCC 1 cut(s) 215
BspPI GGATC 1 cut(s) 235
BsrDI GCAATG 2 cut(s) 387, 409
BsrI ACTGG 1 cut(s) 329
BssECI CCNNGG 1 cut(s) 168
BssMI GATC 4 cut(s) 227, 270, 319, 439
BssT1I CCWWGG 1 cut(s) 168
Bst6I CTCTTC 2 cut(s) 122, 480
BstC8I GCNNGC 2 cut(s) 283, 627
BstDEI CTNAG 2 cut(s) 177, 635
BstDSI CCRYGG 1 cut(s) 168
BstF5I GGATG 1 cut(s) 213
BstKTI GATC 4 cut(s) 230, 273, 322, 442
BstMAI GTCTC 2 cut(s) 98, 477
BstMBI GATC 4 cut(s) 227, 270, 319, 439
BstMWI GCNNNNNNNGC 3 cut(s) 67, 109, 626
BstNSI RCATGY 1 cut(s) 351
BstSFI CTRYAG 1 cut(s) 222
BstV1I GCAGC 2 cut(s) 290, 612
BstX2I RGATCY 2 cut(s) 227, 439
BstYI RGATCY 2 cut(s) 227, 439
BsuRI GGCC 1 cut(s) 215
BtgI CCRYGG 1 cut(s) 168
BtsCI GGATG 1 cut(s) 213
BtsIMutI CAGTG 1 cut(s) 322
Cac8I GCNNGC 2 cut(s) 283, 627
Cfr13I GGNCC 1 cut(s) 121
Csp6I GTAC 1 cut(s) 489
CviAII CATG 5 cut(s) 137, 169, 286, 348, 377
CviQI GTAC 1 cut(s) 489
DdeI CTNAG 2 cut(s) 177, 635
DpnI GATC 4 cut(s) 229, 272, 321, 441
DpnII GATC 4 cut(s) 227, 270, 319, 439
EaeI YGGCCR 1 cut(s) 213
Eam1104I CTCTTC 2 cut(s) 122, 480
EarI CTCTTC 2 cut(s) 122, 480
Eco130I CCWWGG 1 cut(s) 168
Eco32I GATATC 1 cut(s) 295
Eco47I GGWCC 1 cut(s) 121
Eco57I CTGAAG 1 cut(s) 389
EcoRV GATATC 1 cut(s) 295
EcoT14I CCWWGG 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 168
FaeI CATG 5 cut(s) 140, 172, 289, 351, 380
FalI AAGNNNNNCTT 1 cut(s) 618
FaqI GGGAC 1 cut(s) 187
FatI CATG 5 cut(s) 136, 168, 285, 347, 376
Fnu4HI GCNGC 2 cut(s) 279, 601
FokI GGATG 1 cut(s) 220
Fsp4HI GCNGC 2 cut(s) 279, 601
GluI GCNGC 2 cut(s) 279, 601
HaeIII GGCC 1 cut(s) 215
Hin1II CATG 5 cut(s) 140, 172, 289, 351, 380
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 2 cut(s) 373, 470
HphI GGTGA 1 cut(s) 41
Hpy188III TCNNGA 1 cut(s) 547
HpyAV CCTTC 2 cut(s) 112, 307
HpyCH4IV ACGT 1 cut(s) 495
HpyCH4V TGCA 6 cut(s) 260, 285, 380, 394, 414, 600
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 109, 626
HpyF3I CTNAG 2 cut(s) 177, 635
HpySE526I ACGT 1 cut(s) 495
Hsp92II CATG 5 cut(s) 140, 172, 289, 351, 380
Kzo9I GATC 4 cut(s) 227, 270, 319, 439
LmnI GCTCC 1 cut(s) 338
LpnPI CCDG 8 cut(s) 12, 56, 137, 225, 295, 310, 560, 576
Lsp1109I GCAGC 2 cut(s) 290, 612
LweI GCATC 1 cut(s) 14
MaeII ACGT 1 cut(s) 495
MaeIII GTNAC 1 cut(s) 350
MalI GATC 4 cut(s) 229, 272, 321, 441
MboI GATC 4 cut(s) 227, 270, 319, 439
MboII GAAGA 5 cut(s) 86, 139, 382, 467, 595
MflI RGATCY 2 cut(s) 227, 439
MlsI TGGCCA 1 cut(s) 215
MluCI AATT 6 cut(s) 157, 217, 432, 460, 567, 609
MluNI TGGCCA 1 cut(s) 215
MlyI GAGTC 1 cut(s) 479
MmeI TCCRAC 1 cut(s) 122
MnlI CCTC 4 cut(s) 60, 194, 350, 565
Mox20I TGGCCA 1 cut(s) 215
MscI TGGCCA 1 cut(s) 215
MseI TTAA 2 cut(s) 435, 485
MslI CAYNNNNRTG 1 cut(s) 27
Msp20I TGGCCA 1 cut(s) 215
Mva1269I GAATGC 1 cut(s) 602
MwoI GCNNNNNNNGC 3 cut(s) 67, 109, 626
NcoI CCATGG 1 cut(s) 168
NdeII GATC 4 cut(s) 227, 270, 319, 439
NlaIII CATG 5 cut(s) 140, 172, 289, 351, 380
NmuCI GTSAC 1 cut(s) 350
NspI RCATGY 1 cut(s) 351
OliI CACNNNNGTG 1 cut(s) 27
PctI GAATGC 1 cut(s) 602
PfeI GAWTC 1 cut(s) 373
PkrI GCNGC 2 cut(s) 280, 602
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
PspPI GGNCC 1 cut(s) 121
PsuI RGATCY 2 cut(s) 227, 439
RsaI GTAC 1 cut(s) 490
RsaNI GTAC 1 cut(s) 489
RseI CAYNNNNRTG 1 cut(s) 27
SaqAI TTAA 2 cut(s) 435, 485
SatI GCNGC 2 cut(s) 279, 601
Sau3AI GATC 4 cut(s) 227, 270, 319, 439
Sau96I GGNCC 1 cut(s) 121
SchI GAGTC 1 cut(s) 479
SfaNI GCATC 1 cut(s) 14
SfcI CTRYAG 1 cut(s) 222
SinI GGWCC 1 cut(s) 121
SmiMI CAYNNNNRTG 1 cut(s) 27
SmlI CTYRAG 2 cut(s) 62, 98
SmoI CTYRAG 2 cut(s) 62, 98
Sse9I AATT 6 cut(s) 157, 217, 432, 460, 567, 609
StyI CCWWGG 1 cut(s) 168
TaiI ACGT 1 cut(s) 498
TaqI TCGA 2 cut(s) 269, 342
TasI AATT 6 cut(s) 157, 217, 432, 460, 567, 609
TatI WGTACW 1 cut(s) 488
TfiI GAWTC 1 cut(s) 373
Tru1I TTAA 2 cut(s) 435, 485
Tru9I TTAA 2 cut(s) 435, 485
TscAI CASTG 1 cut(s) 329
TseFI GTSAC 1 cut(s) 350
TseI GCWGC 2 cut(s) 278, 600
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 2 cut(s) 125, 398
TspRI CASTG 1 cut(s) 329
VpaK11BI GGWCC 1 cut(s) 121
XapI RAATTY 2 cut(s) 460, 609
XceI RCATGY 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.