Rorug06G0003700

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002011
Physical Location & Seq
Reverse (-)
588187 .. 591503
3317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0003700.1

Sequence Viewer

Length: 591 bp
ATGGCAAATATCGGGTTGCTCACCATCTGGCTCAACAAGAAGATCGTCGAGCCTCTACTCGAAATCCTTCGCAAGGGTGCCGAGCCTAAGCTGTTGGCCTTCTCTGCAGCTCTTGGCTTCACCATTGGATTGTTTCCAATCTGTGGGTCAACTGTGATTATCTGTGGGTTGGCTATTGCATCTCTTGGATCCCACTGCCATGCGCCAACTGTCATGGTGATTAATCTCATTGCTACACCAATTGAACTGAGTTTAATGGTACCGTTCCTGCGCTTTGGTGAATACGTCTCTGGTGGACCTCATTTTCCATTGACTTCTGATGCTTTGAAGAAAGTTTTAACCCTCCAAGCTTCGCGTGAAGTCTTATTAAGTATTGCACATGCGTTGTTGGGATGGCTTGTTGCGGCTCCTTTTATTTTTGCTGCAGTGTATATACTCTCTTTACCATTGTTTAAGGTGTTGGTTCACAAGTTTAGCACGCGTCCTTTAAGCCCAAAGAGATCACTTTCACCAACAGAAGTCAGGATTAAGAGCTGCTTGCAAGAGGGAAGGCAAGAGACAATGGAGGAGTTGGAAGACCTTCTGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

196

Amino Acids

21.34

Weight (kDa)

8.73

Isoelectric Point (pI)

52.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2062 PF09835 19 - 157 8.2e-07 Uncharacterized protein conserved in bacteria (DUF2062)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 259
AccB1I GGYRCC 2 cut(s) 77, 259
AccB7I CCANNNNNTGG 1 cut(s) 143
AccII CGCG 2 cut(s) 355, 481
AciI CCGC 1 cut(s) 404
AclWI GGATC 2 cut(s) 183, 196
AfaI GTAC 1 cut(s) 261
AfiI CCNNNNNNNGG 2 cut(s) 73, 143
AflIII ACRYGT 1 cut(s) 479
AgsI TTSAA 2 cut(s) 245, 328
AluBI AGCT 4 cut(s) 91, 110, 350, 534
AluI AGCT 4 cut(s) 91, 110, 350, 534
Alw26I GTCTC 2 cut(s) 292, 551
AlwI GGATC 2 cut(s) 183, 196
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 3 cut(s) 107, 422, 534
AseI ATTAAT 1 cut(s) 222
Asp700I GAANNNNTTC 2 cut(s) 66, 579
Asp718I GGTACC 1 cut(s) 259
AspLEI GCGC 2 cut(s) 205, 273
AspS9I GGNCC 1 cut(s) 296
AsuHPI GGTGA 5 cut(s) 13, 112, 229, 290, 501
AvaII GGWCC 1 cut(s) 296
BamHI GGATCC 1 cut(s) 188
BanI GGYRCC 2 cut(s) 77, 259
BbsI GAAGAC 1 cut(s) 582
BbvI GCAGC 3 cut(s) 119, 409, 521
BccI CCATC 2 cut(s) 32, 387
BcoDI GTCTC 2 cut(s) 292, 551
BfmI CTRYAG 2 cut(s) 105, 423
BisI GCNGC 4 cut(s) 108, 405, 423, 535
BlsI GCNGC 4 cut(s) 109, 406, 424, 536
Bme18I GGWCC 1 cut(s) 296
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 4 cut(s) 79, 190, 261, 408
BmsI GCATC 2 cut(s) 188, 310
BpiI GAAGAC 1 cut(s) 582
Bpu10I CCTNAGC 1 cut(s) 87
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 143
Bse3DI GCAATG 1 cut(s) 228
BseGI GGATG 1 cut(s) 398
BseLI CCNNNNNNNGG 2 cut(s) 73, 143
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 1 cut(s) 239
BseRI GAGGAG 1 cut(s) 581
BseXI GCAGC 3 cut(s) 119, 409, 521
Bsh1236I CGCG 2 cut(s) 355, 481
BshFI GGCC 1 cut(s) 98
BshNI GGYRCC 2 cut(s) 77, 259
BslI CCNNNNNNNGG 2 cut(s) 73, 143
BsmAI GTCTC 2 cut(s) 292, 551
BsmBI CGTCTC 1 cut(s) 292
BsnI GGCC 1 cut(s) 98
Bsp143I GATC 3 cut(s) 42, 188, 500
BspACI CCGC 1 cut(s) 404
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 1 cut(s) 240
BspFNI CGCG 2 cut(s) 355, 481
BspLI GGNNCC 4 cut(s) 79, 190, 261, 408
BspMAI CTGCAG 2 cut(s) 109, 427
BspPI GGATC 2 cut(s) 183, 196
BspT107I GGYRCC 2 cut(s) 77, 259
BsrDI GCAATG 1 cut(s) 228
BssMI GATC 3 cut(s) 42, 188, 500
Bst4CI ACNGT 3 cut(s) 154, 211, 264
BstC8I GCNNGC 2 cut(s) 479, 539
BstDEI CTNAG 2 cut(s) 87, 248
BstENI CCTNNNNNAGG 1 cut(s) 71
BstF5I GGATG 1 cut(s) 398
BstFNI CGCG 2 cut(s) 355, 481
BstHHI GCGC 2 cut(s) 205, 273
BstKTI GATC 3 cut(s) 45, 191, 503
BstMAI GTCTC 2 cut(s) 292, 551
BstMBI GATC 3 cut(s) 42, 188, 500
BstMWI GCNNNNNNNGC 1 cut(s) 104
BstNSI RCATGY 1 cut(s) 383
BstSFI CTRYAG 2 cut(s) 105, 423
BstUI CGCG 2 cut(s) 355, 481
BstV1I GCAGC 3 cut(s) 119, 409, 521
BstV2I GAAGAC 1 cut(s) 582
BstX2I RGATCY 1 cut(s) 188
BstYI RGATCY 1 cut(s) 188
BsuRI GGCC 1 cut(s) 98
BtsCI GGATG 1 cut(s) 398
BtsI GCAGTG 2 cut(s) 193, 432
BtsIMutI CAGTG 2 cut(s) 193, 432
Cac8I GCNNGC 2 cut(s) 479, 539
CfoI GCGC 2 cut(s) 205, 273
Cfr13I GGNCC 1 cut(s) 296
CseI GACGC 1 cut(s) 470
Csp6I GTAC 1 cut(s) 260
CviAII CATG 3 cut(s) 200, 214, 380
CviQI GTAC 1 cut(s) 260
DdeI CTNAG 2 cut(s) 87, 248
DpnI GATC 3 cut(s) 44, 190, 502
DpnII GATC 3 cut(s) 42, 188, 500
Eco47I GGWCC 1 cut(s) 296
EcoNI CCTNNNNNAGG 1 cut(s) 71
Esp3I CGTCTC 1 cut(s) 292
FaeI CATG 3 cut(s) 203, 217, 383
FaiI YATR 6 cut(s) 201, 215, 381, 432, 434, 589
FalI AAGNNNNNCTT 2 cut(s) 521, 553
FatI CATG 3 cut(s) 199, 213, 379
Fnu4HI GCNGC 4 cut(s) 108, 405, 423, 535
FokI GGATG 1 cut(s) 405
Fsp4HI GCNGC 4 cut(s) 108, 405, 423, 535
GlaI GCGC 2 cut(s) 204, 272
GluI GCNGC 4 cut(s) 108, 405, 423, 535
HaeIII GGCC 1 cut(s) 98
HgaI GACGC 1 cut(s) 470
HhaI GCGC 2 cut(s) 205, 273
Hin1II CATG 3 cut(s) 203, 217, 383
Hin6I GCGC 2 cut(s) 203, 271
HinP1I GCGC 2 cut(s) 203, 271
HincII GTYRAC 1 cut(s) 150
HindII GTYRAC 1 cut(s) 150
HindIII AAGCTT 1 cut(s) 348
HphI GGTGA 5 cut(s) 13, 112, 229, 290, 501
Hpy166II GTNNAC 3 cut(s) 150, 296, 466
Hpy188I TCNGA 1 cut(s) 319
Hpy188III TCNNGA 1 cut(s) 523
Hpy8I GTNNAC 3 cut(s) 150, 296, 466
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 4 cut(s) 77, 109, 543, 590
HpyCH4III ACNGT 3 cut(s) 154, 211, 264
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 5 cut(s) 107, 179, 377, 425, 541
HpyF10VI GCNNNNNNNGC 1 cut(s) 104
HpyF3I CTNAG 2 cut(s) 87, 248
HpySE526I ACGT 1 cut(s) 285
Hsp92II CATG 3 cut(s) 203, 217, 383
HspAI GCGC 2 cut(s) 203, 271
KpnI GGTACC 1 cut(s) 263
Kzo9I GATC 3 cut(s) 42, 188, 500
LmnI GCTCC 1 cut(s) 412
LpnPI CCDG 4 cut(s) 13, 276, 281, 508
Lsp1109I GCAGC 3 cut(s) 119, 409, 521
LweI GCATC 2 cut(s) 188, 310
MaeII ACGT 1 cut(s) 285
MalI GATC 3 cut(s) 44, 190, 502
MboI GATC 3 cut(s) 42, 188, 500
MboII GAAGA 3 cut(s) 52, 340, 587
MfeI CAATTG 1 cut(s) 240
MflI RGATCY 1 cut(s) 188
MluCI AATT 1 cut(s) 240
MluI ACGCGT 1 cut(s) 479
MmeI TCCRAC 1 cut(s) 552
MnlI CCTC 5 cut(s) 63, 309, 353, 538, 559
MroXI GAANNNNTTC 2 cut(s) 66, 579
MseI TTAA 7 cut(s) 222, 254, 338, 368, 453, 488, 528
MslI CAYNNNNRTG 1 cut(s) 198
MunI CAATTG 1 cut(s) 240
MvnI CGCG 2 cut(s) 355, 481
MwoI GCNNNNNNNGC 1 cut(s) 104
NdeII GATC 3 cut(s) 42, 188, 500
NlaIII CATG 3 cut(s) 203, 217, 383
NlaIV GGNNCC 4 cut(s) 79, 190, 261, 408
NmeAIII GCCGAG 1 cut(s) 106
NspI RCATGY 1 cut(s) 383
PdmI GAANNNNTTC 2 cut(s) 66, 579
PflMI CCANNNNNTGG 1 cut(s) 143
PkrI GCNGC 4 cut(s) 109, 406, 424, 536
PshBI ATTAAT 1 cut(s) 222
PspN4I GGNNCC 4 cut(s) 79, 190, 261, 408
PspPI GGNCC 1 cut(s) 296
PstI CTGCAG 2 cut(s) 109, 427
PsuI RGATCY 1 cut(s) 188
RsaI GTAC 1 cut(s) 261
RsaNI GTAC 1 cut(s) 260
RseI CAYNNNNRTG 1 cut(s) 198
SaqAI TTAA 7 cut(s) 222, 254, 338, 368, 453, 488, 528
SatI GCNGC 4 cut(s) 108, 405, 423, 535
Sau3AI GATC 3 cut(s) 42, 188, 500
Sau96I GGNCC 1 cut(s) 296
SetI ASST 8 cut(s) 93, 112, 288, 301, 352, 459, 536, 582
SfaNI GCATC 2 cut(s) 188, 310
SfcI CTRYAG 2 cut(s) 105, 423
SinI GGWCC 1 cut(s) 296
SmiMI CAYNNNNRTG 1 cut(s) 198
Sse9I AATT 1 cut(s) 240
SsiI CCGC 1 cut(s) 404
TaaI ACNGT 3 cut(s) 154, 211, 264
TaiI ACGT 1 cut(s) 288
TaqI TCGA 2 cut(s) 48, 60
TasI AATT 1 cut(s) 240
TauI GCSGC 1 cut(s) 407
Tru1I TTAA 7 cut(s) 222, 254, 338, 368, 453, 488, 528
Tru9I TTAA 7 cut(s) 222, 254, 338, 368, 453, 488, 528
TscAI CASTG 2 cut(s) 200, 432
TseI GCWGC 3 cut(s) 107, 422, 534
TspRI CASTG 2 cut(s) 200, 432
Van91I CCANNNNNTGG 1 cut(s) 143
VpaK11BI GGWCC 1 cut(s) 296
VspI ATTAAT 1 cut(s) 222
XagI CCTNNNNNAGG 1 cut(s) 71
XceI RCATGY 1 cut(s) 383
XmnI GAANNNNTTC 2 cut(s) 66, 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.