FvH4_2g08250

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002011
Physical Location & Seq
Reverse (-)
7166953 .. 7172068
5116 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g08250.t4

Sequence Viewer

Length: 744 bp
ATGAAATCAGGACCAAAGGATGGTTGGCAATCTATCATGCCTCTTTGTTTGAGAGGAAAATCAGCTTCACGTTTTTGCATGTTTCGAAATGTGAAGACAGCAGACTACAGTCCAGGAAATGTACCTGTCTATCTAAATGTTTATGACTTGACACCTGCAAATGGCTATGTTTATTGGGCAGGTGTTGGCATCTTTCACTCTGGAGTTGAAGTCTACGGTGTAGAATATGCCTTTGGAGCCCACGAGTACTCATCAAGTGGTGTCTTTGAGGTTGAACCTCGACAGTGCCCTGGCTTCAAGTTTAGAAGGTCAATTTTCATCGGCACAACATGCTTGGATCCTTTCCAGGTTAGAGAGTTCATGGAGCGTGAATCTGCAAATTACAATGGTGACACATATCACTTGATAATTAAGAACTGCAACCATTTCTGTCACAATATATGTTATAAGCTGACTGGAAAATCAACACCAAAATGGGTCAACCGCCTCGCAAAAATAGGTTCAATGTGCAACTGTATACTCCCAGATGCCCTTAAGTCGACCACTGTTCCACATGACCCCAATTTCCAAGAATATGAAAGTGAAAAAAAGAGGCTAAGAAGTGCCTTCACTTGCTTGTCATCAATCTCAATGCCTCAGAAGGAAGTATCAATGTCATCATTATTTCTACATTCTCACTATAAAGGCTGCTTACCACCGTGGGAGTTTAGAAGGTCTAGAAATGGCTCATTGAAGAAAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

248

Amino Acids

28.08

Weight (kDa)

9.14

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 42 - 177 1.1e-48 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 447
AarI CACCTGC 2 cut(s) 163, 170
Acc36I ACCTGC 2 cut(s) 163, 170
AccB7I CCANNNNNTGG 1 cut(s) 20
AccI GTMKAC 3 cut(s) 213, 517, 539
AciI CCGC 1 cut(s) 484
AclWI GGATC 2 cut(s) 332, 345
AfaI GTAC 2 cut(s) 123, 248
AfiI CCNNNNNNNGG 2 cut(s) 20, 161
AflII CTTAAG 1 cut(s) 533
AgsI TTSAA 5 cut(s) 209, 275, 298, 504, 733
AjnI CCWGG 3 cut(s) 112, 289, 345
AjuI GAANNNNNNNTTGG 2 cut(s) 216, 248
AluBI AGCT 2 cut(s) 65, 451
AluI AGCT 2 cut(s) 65, 451
AlwI GGATC 2 cut(s) 332, 345
ApeKI GCWGC 1 cut(s) 687
AspS9I GGNCC 1 cut(s) 11
AsuHPI GGTGA 1 cut(s) 401
AsuII TTCGAA 1 cut(s) 85
AvaII GGWCC 1 cut(s) 11
BaeGI GKGCMC 1 cut(s) 290
BamHI GGATCC 1 cut(s) 337
BanII GRGCYC 1 cut(s) 241
BauI CACGAG 1 cut(s) 242
BbsI GAAGAC 1 cut(s) 101
BbvI GCAGC 1 cut(s) 674
BccI CCATC 1 cut(s) 14
BciT130I CCWGG 3 cut(s) 114, 291, 347
BfaI CTAG 1 cut(s) 717
BfmI CTRYAG 1 cut(s) 106
BfrI CTTAAG 1 cut(s) 533
BfuAI ACCTGC 2 cut(s) 163, 170
BisI GCNGC 1 cut(s) 688
BlsI GCNGC 1 cut(s) 689
BmcAI AGTACT 1 cut(s) 248
Bme1390I CCNGG 3 cut(s) 114, 291, 347
Bme18I GGWCC 1 cut(s) 11
BmgT120I GGNCC 1 cut(s) 11
BmiI GGNNCC 2 cut(s) 238, 339
BmrFI CCNGG 3 cut(s) 114, 291, 347
BmsI GCATC 2 cut(s) 198, 517
BoxI GACNNNNGTC 1 cut(s) 108
BpiI GAAGAC 1 cut(s) 101
BpmI CTGGAG 1 cut(s) 222
Bpu14I TTCGAA 1 cut(s) 85
BsaJI CCNNGG 2 cut(s) 289, 698
BsaXI ACNNNNNCTCC 2 cut(s) 195, 225
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 161
Bse1I ACTGG 1 cut(s) 460
BseBI CCWGG 3 cut(s) 114, 291, 347
BseDI CCNNGG 2 cut(s) 289, 698
BseGI GGATG 1 cut(s) 25
BseLI CCNNNNNNNGG 2 cut(s) 20, 161
BseMII CTCAG 1 cut(s) 650
BseNI ACTGG 1 cut(s) 460
BseSI GKGCMC 1 cut(s) 290
BseXI GCAGC 1 cut(s) 674
BslI CCNNNNNNNGG 2 cut(s) 20, 161
Bsp119I TTCGAA 1 cut(s) 85
Bsp1286I GDGCHC 2 cut(s) 241, 290
Bsp143I GATC 1 cut(s) 337
BspACI CCGC 1 cut(s) 484
BspCNI CTCAG 1 cut(s) 649
BspLI GGNNCC 2 cut(s) 238, 339
BspMI ACCTGC 2 cut(s) 163, 170
BspPI GGATC 2 cut(s) 332, 345
BspT104I TTCGAA 1 cut(s) 85
BspTI CTTAAG 1 cut(s) 533
BsrI ACTGG 1 cut(s) 460
BssECI CCNNGG 2 cut(s) 289, 698
BssMI GATC 1 cut(s) 337
BssNAI GTATAC 1 cut(s) 518
BssSI CACGAG 1 cut(s) 242
Bst1107I GTATAC 1 cut(s) 518
Bst2BI CACGAG 1 cut(s) 242
Bst2UI CCWGG 3 cut(s) 114, 291, 347
Bst4CI ACNGT 6 cut(s) 110, 218, 285, 515, 547, 699
BstAFI CTTAAG 1 cut(s) 533
BstAPI GCANNNNNTGC 1 cut(s) 330
BstBI TTCGAA 1 cut(s) 85
BstDEI CTNAG 2 cut(s) 596, 636
BstDSI CCRYGG 1 cut(s) 698
BstF5I GGATG 1 cut(s) 25
BstKTI GATC 1 cut(s) 340
BstMBI GATC 1 cut(s) 337
BstMWI GCNNNNNNNGC 2 cut(s) 236, 330
BstNI CCWGG 3 cut(s) 114, 291, 347
BstNSI RCATGY 2 cut(s) 82, 333
BstPAI GACNNNNGTC 1 cut(s) 108
BstSCI CCNGG 3 cut(s) 112, 289, 345
BstSFI CTRYAG 1 cut(s) 106
BstSLI GKGCMC 1 cut(s) 290
BstV1I GCAGC 1 cut(s) 674
BstV2I GAAGAC 1 cut(s) 101
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
BstZ17I GTATAC 1 cut(s) 518
BtgI CCRYGG 1 cut(s) 698
BtsCI GGATG 1 cut(s) 25
BtsIMutI CAGTG 2 cut(s) 290, 543
BveI ACCTGC 2 cut(s) 163, 170
Cfr13I GGNCC 1 cut(s) 11
Csp6I GTAC 2 cut(s) 122, 247
CviAII CATG 5 cut(s) 37, 79, 330, 361, 554
CviJI RGCY 8 cut(s) 65, 165, 239, 294, 451, 595, 687, 726
CviKI_1 RGCY 8 cut(s) 65, 165, 239, 294, 451, 595, 687, 726
CviQI GTAC 2 cut(s) 122, 247
DdeI CTNAG 2 cut(s) 596, 636
DpnI GATC 1 cut(s) 339
DpnII GATC 1 cut(s) 337
Eco24I GRGCYC 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 11
EcoRII CCWGG 3 cut(s) 112, 289, 345
EcoT38I GRGCYC 1 cut(s) 241
FaeI CATG 5 cut(s) 40, 82, 333, 364, 557
FatI CATG 5 cut(s) 36, 78, 329, 360, 553
FblI GTMKAC 3 cut(s) 213, 517, 539
Fnu4HI GCNGC 1 cut(s) 688
FokI GGATG 1 cut(s) 32
FriOI GRGCYC 1 cut(s) 241
Fsp4HI GCNGC 1 cut(s) 688
FspBI CTAG 1 cut(s) 717
GluI GCNGC 1 cut(s) 688
GsuI CTGGAG 1 cut(s) 222
Hin1II CATG 5 cut(s) 40, 82, 333, 364, 557
HincII GTYRAC 2 cut(s) 481, 540
HindII GTYRAC 2 cut(s) 481, 540
HinfI GANTC 1 cut(s) 371
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 4 cut(s) 214, 481, 518, 540
Hpy188I TCNGA 1 cut(s) 639
Hpy188III TCNNGA 3 cut(s) 9, 201, 717
Hpy8I GTNNAC 4 cut(s) 214, 481, 518, 540
HpyAV CCTTC 4 cut(s) 300, 616, 634, 705
HpyCH4III ACNGT 6 cut(s) 110, 218, 285, 515, 547, 699
HpyCH4IV ACGT 1 cut(s) 70
HpyCH4V TGCA 5 cut(s) 78, 158, 377, 420, 510
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 330
HpyF3I CTNAG 2 cut(s) 596, 636
HpySE526I ACGT 1 cut(s) 70
Hsp92II CATG 5 cut(s) 40, 82, 333, 364, 557
Kzo9I GATC 1 cut(s) 337
LmnI GCTCC 2 cut(s) 236, 364
Lsp1109I GCAGC 1 cut(s) 674
LweI GCATC 2 cut(s) 198, 517
MaeI CTAG 1 cut(s) 717
MaeII ACGT 1 cut(s) 70
MaeIII GTNAC 2 cut(s) 389, 431
MalI GATC 1 cut(s) 339
MboI GATC 1 cut(s) 337
MboII GAAGA 1 cut(s) 106
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 2 cut(s) 241, 290
MluCI AATT 4 cut(s) 312, 379, 408, 562
MnlI CCTC 7 cut(s) 47, 51, 262, 288, 497, 585, 645
MseI TTAA 2 cut(s) 411, 534
MslI CAYNNNNRTG 1 cut(s) 472
MspCI CTTAAG 1 cut(s) 533
MspR9I CCNGG 3 cut(s) 114, 291, 347
MvaI CCWGG 3 cut(s) 114, 291, 347
MwoI GCNNNNNNNGC 2 cut(s) 236, 330
NdeII GATC 1 cut(s) 337
NlaIII CATG 5 cut(s) 40, 82, 333, 364, 557
NlaIV GGNNCC 2 cut(s) 238, 339
NmuCI GTSAC 2 cut(s) 389, 431
NspI RCATGY 2 cut(s) 82, 333
NspV TTCGAA 1 cut(s) 85
PaqCI CACCTGC 2 cut(s) 163, 170
PfeI GAWTC 1 cut(s) 371
PflMI CCANNNNNTGG 1 cut(s) 20
PfoI TCCNGGA 1 cut(s) 112
PkrI GCNGC 1 cut(s) 689
PshAI GACNNNNGTC 1 cut(s) 108
PsiI TTATAA 1 cut(s) 447
Psp6I CCWGG 3 cut(s) 112, 289, 345
PspGI CCWGG 3 cut(s) 112, 289, 345
PspN4I GGNNCC 2 cut(s) 238, 339
PspPI GGNCC 1 cut(s) 11
PsuI RGATCY 1 cut(s) 337
RsaI GTAC 2 cut(s) 123, 248
RsaNI GTAC 2 cut(s) 122, 247
RseI CAYNNNNRTG 1 cut(s) 472
SalI GTCGAC 1 cut(s) 538
SaqAI TTAA 2 cut(s) 411, 534
SatI GCNGC 1 cut(s) 688
Sau3AI GATC 1 cut(s) 337
Sau96I GGNCC 1 cut(s) 11
ScaI AGTACT 1 cut(s) 248
ScrFI CCNGG 3 cut(s) 114, 291, 347
SduI GDGCHC 2 cut(s) 241, 290
SfaNI GCATC 2 cut(s) 198, 517
SfcI CTRYAG 1 cut(s) 106
SfuI TTCGAA 1 cut(s) 85
SinI GGWCC 1 cut(s) 11
SmiMI CAYNNNNRTG 1 cut(s) 472
SmlI CTYRAG 1 cut(s) 533
SmoI CTYRAG 1 cut(s) 533
Sse9I AATT 4 cut(s) 312, 379, 408, 562
SsiI CCGC 1 cut(s) 484
SspMI CTAG 1 cut(s) 717
StyD4I CCNGG 3 cut(s) 112, 289, 345
TaaI ACNGT 6 cut(s) 110, 218, 285, 515, 547, 699
TaiI ACGT 1 cut(s) 73
TaqI TCGA 3 cut(s) 85, 280, 539
TasI AATT 4 cut(s) 312, 379, 408, 562
TatI WGTACW 1 cut(s) 246
TfiI GAWTC 1 cut(s) 371
Tru1I TTAA 2 cut(s) 411, 534
Tru9I TTAA 2 cut(s) 411, 534
TscAI CASTG 2 cut(s) 290, 550
TseFI GTSAC 2 cut(s) 389, 431
TseI GCWGC 1 cut(s) 687
Tsp45I GTSAC 2 cut(s) 389, 431
TspDTI ATGAA 4 cut(s) 17, 307, 349, 591
TspRI CASTG 2 cut(s) 290, 550
Van91I CCANNNNNTGG 1 cut(s) 20
Vha464I CTTAAG 1 cut(s) 533
VpaK11BI GGWCC 1 cut(s) 11
XbaI TCTAGA 1 cut(s) 716
XceI RCATGY 2 cut(s) 82, 333
XcmI CCANNNNNNNNNTGG 1 cut(s) 21
XmiI GTMKAC 3 cut(s) 213, 517, 539
XspI CTAG 1 cut(s) 717
ZrmI AGTACT 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.