MD15G1029100.v1.1

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002011
Physical Location & Seq
Reverse (-)
1777360 .. 1780017
2658 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1029100.v1.1.491

Sequence Viewer

Length: 744 bp
ATGAAATTTGAGTTAAAGAAGAGATGGAGATCCATTTCCCCGCTTCAATTGAAACCCAAATCAGCGGGACGTTTTTGTTTATTTCCCAAATCAAAGTCCGATAACGCTGAATCAGGAAAAGCACCAGTTTATCTCAATGTATATGATTTGACTCCCATGAATGGCTATGTCTATTGGGCAGGCTTTGGAATTTTTCACTCTGGTGTTGAAGTTCATGGTGTAGAATATGCATTCGGAGCACATGACTACCCTACAAGTGGCGTTTTTGAGGTTGAACCTCGCCAATGCCCAGGGTTCAAGTTCAGGAAGTCTATTTTAATCGGAACAACATGCTTGGACCCTACTCAGGTTCGAGAGTTTATGGAGCGCCAGTCTTTGAACTATAACGGTGATACATATCACCTGATTGTCAAGAACTGCAACCATTTCTGCAGGGATATTTGTCGCAAGCTCACTGGAAAATCAATTCCTAAATGGGTGAATCGACTGGCAAGAATAGGTTCAGTATGCAACTGTGTACTTCCGGAATCTCTGAAGATTTCCGCTGTACAACACGACCCAAACTGCCAACCATACGACAGCGAGAAGAGGAGCTTGAGAAGCGCCTTCAGTTGCCTGTCTTCCATCTCAATGCGGCAGAAGCAATTATCATCATCTTCGTTATTTCTGCAGTCGCCCCTGAAAGGCTGCTTACCGCCATGGGAATTGCGAAGGTCGTTGAATGGTTCGTTGAAAGAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

248

Amino Acids

28.08

Weight (kDa)

9.49

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 42 - 177 5e-51 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 523
AciI CCGC 5 cut(s) 41, 65, 543, 634, 695
AclWI GGATC 1 cut(s) 24
AcsI RAATTY 2 cut(s) 5, 189
AcuI CTGAAG 2 cut(s) 554, 592
AfaI GTAC 2 cut(s) 519, 549
AfiI CCNNNNNNNGG 4 cut(s) 161, 257, 346, 683
AgsI TTSAA 8 cut(s) 47, 52, 209, 275, 298, 379, 721, 733
AjnI CCWGG 1 cut(s) 289
AleI CACNNNNGTG 1 cut(s) 201
AluBI AGCT 2 cut(s) 451, 594
AluI AGCT 2 cut(s) 451, 594
Alw21I GWGCWC 1 cut(s) 241
AlwI GGATC 1 cut(s) 24
Aor13HI TCCGGA 1 cut(s) 523
ApeKI GCWGC 1 cut(s) 687
ApoI RAATTY 2 cut(s) 5, 189
Asp700I GAANNNNTTC 1 cut(s) 499
AspLEI GCGC 2 cut(s) 369, 605
AspS9I GGNCC 1 cut(s) 337
AsuHPI GGTGA 3 cut(s) 392, 401, 490
AvaII GGWCC 1 cut(s) 337
BbsI GAAGAC 1 cut(s) 612
Bbv12I GWGCWC 1 cut(s) 241
BbvI GCAGC 1 cut(s) 674
BccI CCATC 2 cut(s) 18, 632
BciT130I CCWGG 1 cut(s) 291
BfmI CTRYAG 2 cut(s) 430, 668
BfoI RGCGCY 2 cut(s) 370, 606
BisI GCNGC 2 cut(s) 635, 688
BlsI GCNGC 2 cut(s) 636, 689
Bme1390I CCNGG 1 cut(s) 291
Bme18I GGWCC 1 cut(s) 337
BmgT120I GGNCC 1 cut(s) 337
BmiI GGNNCC 1 cut(s) 339
BmrFI CCNGG 1 cut(s) 291
BpiI GAAGAC 1 cut(s) 612
BpuEI CTTGAG 1 cut(s) 616
BsaBI GATNNNNATC 2 cut(s) 28, 396
BsaJI CCNNGG 3 cut(s) 289, 290, 698
BsaWI WCCGGW 1 cut(s) 523
BsaXI ACNNNNNCTCC 2 cut(s) 356, 386
Bsc4I CCNNNNNNNGG 4 cut(s) 161, 257, 346, 683
Bse1I ACTGG 4 cut(s) 125, 370, 460, 492
Bse8I GATNNNNATC 2 cut(s) 28, 396
BseAI TCCGGA 1 cut(s) 523
BseBI CCWGG 1 cut(s) 291
BseDI CCNNGG 3 cut(s) 289, 290, 698
BseJI GATNNNNATC 2 cut(s) 28, 396
BseLI CCNNNNNNNGG 4 cut(s) 161, 257, 346, 683
BseMII CTCAG 1 cut(s) 359
BseNI ACTGG 4 cut(s) 125, 370, 460, 492
BseRI GAGGAG 1 cut(s) 604
BseXI GCAGC 1 cut(s) 674
BsiHKAI GWGCWC 1 cut(s) 241
BsiSI CCGG 1 cut(s) 524
BslFI GGGAC 1 cut(s) 81
BslI CCNNNNNNNGG 4 cut(s) 161, 257, 346, 683
BsmFI GGGAC 1 cut(s) 81
BsmI GAATGC 1 cut(s) 230
Bsp1286I GDGCHC 1 cut(s) 241
Bsp13I TCCGGA 1 cut(s) 523
Bsp1407I TGTACA 1 cut(s) 547
Bsp143I GATC 1 cut(s) 29
Bsp19I CCATGG 1 cut(s) 698
BspACI CCGC 5 cut(s) 41, 65, 543, 634, 695
BspCNI CTCAG 1 cut(s) 358
BspEI TCCGGA 1 cut(s) 523
BspLI GGNNCC 1 cut(s) 339
BspMAI CTGCAG 2 cut(s) 434, 672
BspPI GGATC 1 cut(s) 24
BsrGI TGTACA 1 cut(s) 547
BsrI ACTGG 4 cut(s) 125, 370, 460, 492
BssECI CCNNGG 3 cut(s) 289, 290, 698
BssMI GATC 1 cut(s) 29
BssT1I CCWWGG 1 cut(s) 698
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 2 cut(s) 389, 515
Bst6I CTCTTC 2 cut(s) 14, 581
BstAUI TGTACA 1 cut(s) 547
BstC8I GCNNGC 2 cut(s) 181, 449
BstDEI CTNAG 1 cut(s) 345
BstDSI CCRYGG 1 cut(s) 698
BstH2I RGCGCY 2 cut(s) 370, 606
BstHHI GCGC 2 cut(s) 369, 605
BstKTI GATC 1 cut(s) 32
BstMBI GATC 1 cut(s) 29
BstMWI GCNNNNNNNGC 3 cut(s) 236, 600, 640
BstNI CCWGG 1 cut(s) 291
BstNSI RCATGY 1 cut(s) 333
BstSCI CCNGG 1 cut(s) 289
BstSFI CTRYAG 2 cut(s) 430, 668
BstV1I GCAGC 1 cut(s) 674
BstV2I GAAGAC 1 cut(s) 612
BstX2I RGATCY 1 cut(s) 29
BstYI RGATCY 1 cut(s) 29
BtgI CCRYGG 1 cut(s) 698
BtsIMutI CAGTG 1 cut(s) 453
Cac8I GCNNGC 2 cut(s) 181, 449
CfoI GCGC 2 cut(s) 369, 605
Cfr13I GGNCC 1 cut(s) 337
Csp6I GTAC 2 cut(s) 518, 548
CviAII CATG 5 cut(s) 157, 215, 242, 330, 699
CviJI RGCY 5 cut(s) 165, 183, 451, 594, 687
CviKI_1 RGCY 5 cut(s) 165, 183, 451, 594, 687
CviQI GTAC 2 cut(s) 518, 548
DdeI CTNAG 1 cut(s) 345
DpnI GATC 1 cut(s) 31
DpnII GATC 1 cut(s) 29
Eam1104I CTCTTC 2 cut(s) 14, 581
EarI CTCTTC 2 cut(s) 14, 581
Eco130I CCWWGG 1 cut(s) 698
Eco47I GGWCC 1 cut(s) 337
Eco57I CTGAAG 2 cut(s) 554, 592
EcoRII CCWGG 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 698
EcoT22I ATGCAT 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 698
FaeI CATG 5 cut(s) 160, 218, 245, 333, 702
FalI AAGNNNNNCTT 2 cut(s) 578, 610
FaqI GGGAC 1 cut(s) 81
FatI CATG 5 cut(s) 156, 214, 241, 329, 698
FauI CCCGC 2 cut(s) 48, 58
Fnu4HI GCNGC 2 cut(s) 635, 688
Fsp4HI GCNGC 2 cut(s) 635, 688
GlaI GCGC 2 cut(s) 368, 604
GluI GCNGC 2 cut(s) 635, 688
HaeII RGCGCY 2 cut(s) 370, 606
HapII CCGG 1 cut(s) 524
HhaI GCGC 2 cut(s) 369, 605
Hin1II CATG 5 cut(s) 160, 218, 245, 333, 702
Hin6I GCGC 2 cut(s) 367, 603
HinP1I GCGC 2 cut(s) 367, 603
HinfI GANTC 4 cut(s) 110, 151, 481, 527
HpaII CCGG 1 cut(s) 524
HphI GGTGA 3 cut(s) 392, 401, 490
Hpy166II GTNNAC 1 cut(s) 518
Hpy188I TCNGA 4 cut(s) 100, 236, 323, 534
Hpy188III TCNNGA 5 cut(s) 114, 304, 353, 412, 524
Hpy8I GTNNAC 1 cut(s) 518
HpyAV CCTTC 2 cut(s) 616, 705
HpyCH4III ACNGT 2 cut(s) 389, 515
HpyCH4IV ACGT 1 cut(s) 70
HpyCH4V TGCA 5 cut(s) 230, 420, 432, 510, 670
HpyF10VI GCNNNNNNNGC 3 cut(s) 236, 600, 640
HpyF3I CTNAG 1 cut(s) 345
HpySE526I ACGT 1 cut(s) 70
Hsp92II CATG 5 cut(s) 160, 218, 245, 333, 702
HspAI GCGC 2 cut(s) 367, 603
Kpn2I TCCGGA 1 cut(s) 523
Kzo9I GATC 1 cut(s) 29
LmnI GCTCC 3 cut(s) 236, 364, 591
Lsp1109I GCAGC 1 cut(s) 674
MaeII ACGT 1 cut(s) 70
MalI GATC 1 cut(s) 31
MboI GATC 1 cut(s) 29
MboII GAAGA 5 cut(s) 31, 547, 598, 612, 648
MfeI CAATTG 1 cut(s) 47
MflI RGATCY 1 cut(s) 29
MhlI GDGCHC 1 cut(s) 241
MluCI AATT 6 cut(s) 5, 47, 189, 465, 644, 704
MlyI GAGTC 1 cut(s) 145
MnlI CCTC 3 cut(s) 262, 288, 582
Mph1103I ATGCAT 1 cut(s) 232
MroI TCCGGA 1 cut(s) 523
MroXI GAANNNNTTC 1 cut(s) 499
MseI TTAA 2 cut(s) 14, 317
MslI CAYNNNNRTG 2 cut(s) 201, 629
MspA1I CMGCKG 2 cut(s) 65, 545
MspI CCGG 1 cut(s) 524
MspR9I CCNGG 1 cut(s) 291
MunI CAATTG 1 cut(s) 47
Mva1269I GAATGC 1 cut(s) 230
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 3 cut(s) 236, 600, 640
NcoI CCATGG 1 cut(s) 698
NdeII GATC 1 cut(s) 29
NlaIII CATG 5 cut(s) 160, 218, 245, 333, 702
NlaIV GGNNCC 1 cut(s) 339
NsiI ATGCAT 1 cut(s) 232
NspI RCATGY 1 cut(s) 333
OliI CACNNNNGTG 1 cut(s) 201
PasI CCCWGGG 1 cut(s) 290
PctI GAATGC 1 cut(s) 230
PdmI GAANNNNTTC 1 cut(s) 499
PfeI GAWTC 3 cut(s) 110, 481, 527
PkrI GCNGC 2 cut(s) 636, 689
PleI GAGTC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 145
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 1 cut(s) 339
PspPI GGNCC 1 cut(s) 337
PstI CTGCAG 2 cut(s) 434, 672
PsuI RGATCY 1 cut(s) 29
RsaI GTAC 2 cut(s) 519, 549
RsaNI GTAC 2 cut(s) 518, 548
RseI CAYNNNNRTG 2 cut(s) 201, 629
SaqAI TTAA 2 cut(s) 14, 317
SatI GCNGC 2 cut(s) 635, 688
Sau3AI GATC 1 cut(s) 29
Sau96I GGNCC 1 cut(s) 337
SchI GAGTC 1 cut(s) 145
ScrFI CCNGG 1 cut(s) 291
SduI GDGCHC 1 cut(s) 241
SfcI CTRYAG 2 cut(s) 430, 668
SinI GGWCC 1 cut(s) 337
SmiMI CAYNNNNRTG 2 cut(s) 201, 629
SmlI CTYRAG 1 cut(s) 595
SmoI CTYRAG 1 cut(s) 595
Sse9I AATT 6 cut(s) 5, 47, 189, 465, 644, 704
SsiI CCGC 5 cut(s) 41, 65, 543, 634, 695
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 1 cut(s) 698
TaaI ACNGT 2 cut(s) 389, 515
TaiI ACGT 1 cut(s) 73
TaqI TCGA 2 cut(s) 352, 484
TasI AATT 6 cut(s) 5, 47, 189, 465, 644, 704
TatI WGTACW 2 cut(s) 517, 547
TauI GCSGC 1 cut(s) 637
TfiI GAWTC 3 cut(s) 110, 481, 527
Tru1I TTAA 2 cut(s) 14, 317
Tru9I TTAA 2 cut(s) 14, 317
TscAI CASTG 1 cut(s) 460
TseI GCWGC 1 cut(s) 687
TspDTI ATGAA 3 cut(s) 17, 173, 203
TspRI CASTG 1 cut(s) 460
VpaK11BI GGWCC 1 cut(s) 337
XapI RAATTY 2 cut(s) 5, 189
XceI RCATGY 1 cut(s) 333
XmnI GAANNNNTTC 1 cut(s) 499
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.