MD10G1081000.v1.1

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002011
Physical Location & Seq
Forward (+)
11930877 .. 11934713
3837 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1081000.v1.1.491

Sequence Viewer

Length: 744 bp
ATGAAATCAGGACCAAAGGATGGCTGGCACTCTATTATGCCTCTTTGTTTGAGAGGCAAAGCAGCCTCACGTTTTTGCATGTTTCCAAAAGTGAAGGCTGAAGACTACATTCAAGGGAGCGCACCTGTCTATCTGAATGTATATGACTTGACACCAGCAAATGGCTATGTCTATTGGGCAGGCCTTGGCATCTTTCACTCCGGGGTAGAAGTTCATGGGGTGGAATATGCCTTTGGAGCCCATGAGTACTCATCAAGCGGTGTCTTTGAGGTTGAACCTCGACAATGCCCTGGCTTCAAGTTTAGAAGGTCAATTTTCATTGGCATGACATGCTTGGATCCTTTCCAGGTTAGAGAGTTCATGGAGCGTCAATCTGCCATCTACATTGGTGACTCATACCACTTGATTGTCAAGAACTGCAACCATTTCTGTGAAGATATATGTTATAAGCTGACTGGGAAGCGAATACCCAAATGGGTCAATCGACTCGCAAGAATAGGTTCTCTGTGCAACTGTATACTCCCAGACGCCCTAAAGACGACCACTATTCCTCATGACCCCAATTTCGAAGGATGCGAAAGTGAAAAAAAGAGACTAAGAAGTGCCTTCACTTGCTTGTCATCAATGTCGATGCCACAGAGGGAAGTATCAATGTCGTCATTGTTTCTACATTCTCACTATAAAGGCTGCCTACCACCATGGGAATTTAAAAGGTCTAGAAATGGCTCATTGAAGGAAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

248

Amino Acids

28.05

Weight (kDa)

8.87

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 42 - 177 2e-48 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 447
AccB7I CCANNNNNTGG 2 cut(s) 20, 161
AccI GTMKAC 1 cut(s) 517
AciI CCGC 1 cut(s) 258
AclWI GGATC 2 cut(s) 332, 345
AcsI RAATTY 1 cut(s) 704
AcuI CTGAAG 1 cut(s) 120
AcyI GRCGYC 1 cut(s) 528
AfaI GTAC 1 cut(s) 248
AfiI CCNNNNNNNGG 2 cut(s) 20, 161
AgsI TTSAA 4 cut(s) 113, 275, 298, 733
AjnI CCWGG 2 cut(s) 289, 345
AjuI GAANNNNNNNTTGG 2 cut(s) 216, 248
AluBI AGCT 1 cut(s) 451
AluI AGCT 1 cut(s) 451
Alw26I GTCTC 1 cut(s) 586
AlwI GGATC 2 cut(s) 332, 345
AoxI GGCC 1 cut(s) 181
ApeKI GCWGC 2 cut(s) 62, 687
ApoI RAATTY 1 cut(s) 704
ArsI GACNNNNNNTTYG 2 cut(s) 548, 580
Asp700I GAANNNNTTC 1 cut(s) 499
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 11
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 1 cut(s) 401
AsuII TTCGAA 1 cut(s) 567
AvaII GGWCC 1 cut(s) 11
BamHI GGATCC 1 cut(s) 337
BanII GRGCYC 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 108
BbvI GCAGC 2 cut(s) 74, 674
BccI CCATC 2 cut(s) 14, 386
BciT130I CCWGG 2 cut(s) 291, 347
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 1 cut(s) 586
BfaI CTAG 1 cut(s) 717
BisI GCNGC 2 cut(s) 63, 688
BlsI GCNGC 2 cut(s) 64, 689
BmcAI AGTACT 1 cut(s) 248
Bme1390I CCNGG 3 cut(s) 202, 291, 347
Bme18I GGWCC 1 cut(s) 11
BmgT120I GGNCC 1 cut(s) 11
BmiI GGNNCC 2 cut(s) 238, 339
BmrFI CCNGG 3 cut(s) 202, 291, 347
BmrI ACTGGG 1 cut(s) 465
BmsI GCATC 3 cut(s) 198, 563, 621
BmuI ACTGGG 1 cut(s) 465
BpiI GAAGAC 1 cut(s) 108
Bpu14I TTCGAA 1 cut(s) 567
BpuMI CCSGG 1 cut(s) 202
BsaHI GRCGYC 1 cut(s) 528
BsaJI CCNNGG 4 cut(s) 184, 201, 289, 698
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 161
Bse1I ACTGG 1 cut(s) 460
BseBI CCWGG 2 cut(s) 291, 347
BseDI CCNNGG 4 cut(s) 184, 201, 289, 698
BseGI GGATG 2 cut(s) 25, 578
BseLI CCNNNNNNNGG 2 cut(s) 20, 161
BseNI ACTGG 1 cut(s) 460
BseXI GCAGC 2 cut(s) 74, 674
BshFI GGCC 1 cut(s) 183
BsiSI CCGG 1 cut(s) 201
BslI CCNNNNNNNGG 2 cut(s) 20, 161
BsmAI GTCTC 1 cut(s) 586
BsnI GGCC 1 cut(s) 183
Bsp119I TTCGAA 1 cut(s) 567
Bsp1286I GDGCHC 1 cut(s) 241
Bsp143I GATC 1 cut(s) 337
Bsp19I CCATGG 1 cut(s) 698
BspACI CCGC 1 cut(s) 258
BspANI GGCC 1 cut(s) 183
BspHI TCATGA 1 cut(s) 553
BspLI GGNNCC 2 cut(s) 238, 339
BspPI GGATC 2 cut(s) 332, 345
BspT104I TTCGAA 1 cut(s) 567
BsrI ACTGG 1 cut(s) 460
BssECI CCNNGG 4 cut(s) 184, 201, 289, 698
BssMI GATC 1 cut(s) 337
BssNAI GTATAC 1 cut(s) 518
BssNI GRCGYC 1 cut(s) 528
BssT1I CCWWGG 2 cut(s) 184, 698
Bst1107I GTATAC 1 cut(s) 518
Bst2UI CCWGG 2 cut(s) 291, 347
Bst4CI ACNGT 1 cut(s) 515
BstACI GRCGYC 1 cut(s) 528
BstAPI GCANNNNNTGC 1 cut(s) 330
BstBI TTCGAA 1 cut(s) 567
BstC8I GCNNGC 2 cut(s) 26, 181
BstDEI CTNAG 1 cut(s) 596
BstDSI CCRYGG 1 cut(s) 698
BstF5I GGATG 2 cut(s) 25, 578
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 1 cut(s) 340
BstMAI GTCTC 1 cut(s) 586
BstMBI GATC 1 cut(s) 337
BstMWI GCNNNNNNNGC 2 cut(s) 236, 330
BstNI CCWGG 2 cut(s) 291, 347
BstNSI RCATGY 2 cut(s) 82, 333
BstSCI CCNGG 3 cut(s) 200, 289, 345
BstV1I GCAGC 2 cut(s) 74, 674
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
BstZ17I GTATAC 1 cut(s) 518
BsuRI GGCC 1 cut(s) 183
BtgI CCRYGG 1 cut(s) 698
BtsCI GGATG 2 cut(s) 25, 578
Cac8I GCNNGC 2 cut(s) 26, 181
CciI TCATGA 1 cut(s) 553
CfoI GCGC 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 11
CseI GACGC 2 cut(s) 356, 536
Csp6I GTAC 1 cut(s) 247
CviAII CATG 8 cut(s) 79, 215, 242, 325, 330, 361, 554, 699
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 1 cut(s) 596
DpnI GATC 1 cut(s) 339
DpnII GATC 1 cut(s) 337
DraI TTTAAA 1 cut(s) 709
Eco130I CCWWGG 2 cut(s) 184, 698
Eco147I AGGCCT 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 11
Eco57I CTGAAG 1 cut(s) 120
EcoRII CCWGG 2 cut(s) 289, 345
EcoT14I CCWWGG 2 cut(s) 184, 698
EcoT38I GRGCYC 1 cut(s) 241
ErhI CCWWGG 2 cut(s) 184, 698
FaeI CATG 8 cut(s) 82, 218, 245, 328, 333, 364, 557, 702
FatI CATG 8 cut(s) 78, 214, 241, 324, 329, 360, 553, 698
FblI GTMKAC 1 cut(s) 517
Fnu4HI GCNGC 2 cut(s) 63, 688
FokI GGATG 2 cut(s) 32, 585
FriOI GRGCYC 1 cut(s) 241
Fsp4HI GCNGC 2 cut(s) 63, 688
FspBI CTAG 1 cut(s) 717
GlaI GCGC 1 cut(s) 121
GluI GCNGC 2 cut(s) 63, 688
HaeIII GGCC 1 cut(s) 183
HapII CCGG 1 cut(s) 201
HgaI GACGC 2 cut(s) 356, 536
HhaI GCGC 1 cut(s) 122
Hin1I GRCGYC 1 cut(s) 528
Hin1II CATG 8 cut(s) 82, 218, 245, 328, 333, 364, 557, 702
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HinfI GANTC 2 cut(s) 392, 486
HpaII CCGG 1 cut(s) 201
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 1 cut(s) 518
Hpy188I TCNGA 1 cut(s) 135
Hpy188III TCNNGA 4 cut(s) 9, 412, 554, 717
Hpy8I GTNNAC 1 cut(s) 518
HpyAV CCTTC 6 cut(s) 88, 300, 563, 616, 727, 731
HpyCH4III ACNGT 1 cut(s) 515
HpyCH4IV ACGT 1 cut(s) 70
HpyCH4V TGCA 3 cut(s) 78, 420, 510
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 330
HpyF3I CTNAG 1 cut(s) 596
HpySE526I ACGT 1 cut(s) 70
Hsp92I GRCGYC 1 cut(s) 528
Hsp92II CATG 8 cut(s) 82, 218, 245, 328, 333, 364, 557, 702
HspAI GCGC 1 cut(s) 120
Kzo9I GATC 1 cut(s) 337
LmnI GCTCC 3 cut(s) 117, 236, 364
Lsp1109I GCAGC 2 cut(s) 74, 674
LweI GCATC 3 cut(s) 198, 563, 621
MaeI CTAG 1 cut(s) 717
MaeII ACGT 1 cut(s) 70
MaeIII GTNAC 1 cut(s) 389
MalI GATC 1 cut(s) 339
MboI GATC 1 cut(s) 337
MboII GAAGA 2 cut(s) 113, 446
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 1 cut(s) 241
MluCI AATT 3 cut(s) 312, 562, 704
MlyI GAGTC 2 cut(s) 386, 480
MnlI CCTC 7 cut(s) 47, 51, 76, 262, 288, 561, 633
MroXI GAANNNNTTC 1 cut(s) 499
MseI TTAA 1 cut(s) 708
MslI CAYNNNNRTG 2 cut(s) 323, 429
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 3 cut(s) 202, 291, 347
MvaI CCWGG 2 cut(s) 291, 347
MwoI GCNNNNNNNGC 2 cut(s) 236, 330
NciI CCSGG 1 cut(s) 202
NcoI CCATGG 1 cut(s) 698
NdeII GATC 1 cut(s) 337
NlaIII CATG 8 cut(s) 82, 218, 245, 328, 333, 364, 557, 702
NlaIV GGNNCC 2 cut(s) 238, 339
NmuCI GTSAC 1 cut(s) 389
NspI RCATGY 2 cut(s) 82, 333
NspV TTCGAA 1 cut(s) 567
PagI TCATGA 1 cut(s) 553
PceI AGGCCT 1 cut(s) 183
PcsI WCGNNNNNNNCGW 1 cut(s) 573
PdmI GAANNNNTTC 1 cut(s) 499
PflMI CCANNNNNTGG 2 cut(s) 20, 161
PkrI GCNGC 2 cut(s) 64, 689
PleI GAGTC 2 cut(s) 386, 480
PpsI GAGTC 2 cut(s) 386, 480
PsiI TTATAA 1 cut(s) 447
Psp6I CCWGG 2 cut(s) 289, 345
PspGI CCWGG 2 cut(s) 289, 345
PspN4I GGNNCC 2 cut(s) 238, 339
PspPI GGNCC 1 cut(s) 11
PsuI RGATCY 1 cut(s) 337
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
RseI CAYNNNNRTG 2 cut(s) 323, 429
SaqAI TTAA 1 cut(s) 708
SatI GCNGC 2 cut(s) 63, 688
Sau3AI GATC 1 cut(s) 337
Sau96I GGNCC 1 cut(s) 11
ScaI AGTACT 1 cut(s) 248
SchI GAGTC 2 cut(s) 386, 480
ScrFI CCNGG 3 cut(s) 202, 291, 347
SduI GDGCHC 1 cut(s) 241
SfaNI GCATC 3 cut(s) 198, 563, 621
SfuI TTCGAA 1 cut(s) 567
SinI GGWCC 1 cut(s) 11
SmiMI CAYNNNNRTG 2 cut(s) 323, 429
Sse9I AATT 3 cut(s) 312, 562, 704
SseBI AGGCCT 1 cut(s) 183
SsiI CCGC 1 cut(s) 258
SspMI CTAG 1 cut(s) 717
StuI AGGCCT 1 cut(s) 183
StyD4I CCNGG 3 cut(s) 200, 289, 345
StyI CCWWGG 2 cut(s) 184, 698
TaaI ACNGT 1 cut(s) 515
TaiI ACGT 1 cut(s) 73
TaqI TCGA 4 cut(s) 280, 484, 567, 629
TasI AATT 3 cut(s) 312, 562, 704
TatI WGTACW 1 cut(s) 246
Tru1I TTAA 1 cut(s) 708
Tru9I TTAA 1 cut(s) 708
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 2 cut(s) 62, 687
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 4 cut(s) 17, 203, 307, 349
Van91I CCANNNNNTGG 2 cut(s) 20, 161
VpaK11BI GGWCC 1 cut(s) 11
XapI RAATTY 1 cut(s) 704
XbaI TCTAGA 1 cut(s) 716
XceI RCATGY 2 cut(s) 82, 333
XcmI CCANNNNNNNNNTGG 1 cut(s) 21
XmiI GTMKAC 1 cut(s) 517
XmnI GAANNNNTTC 1 cut(s) 499
XspI CTAG 1 cut(s) 717
ZrmI AGTACT 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.