MD08G1032400.v1.1

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002011
Physical Location & Seq
Reverse (-)
2310145 .. 2312835
2691 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1032400.v1.1.491

Sequence Viewer

Length: 744 bp
ATGAAATTTGAATTGAAGAAGAGATGGAGATCCGTTGCCCCCCTTCAATTGAAGAACAAATCCGCGGGGCGTTTTTGTTTATTTCCCAAATCAAAATCGGATAGCGCTGAATCAGGAAAAGCACCAGTTTATCTCAATGTATATGATCTGACTCCCATGAATGGCTATGTCTATTGGGCTGGCTTTGGAATTTTTCACTCTGGTGTTGAAGTTCATGGTGTGGAATATGCATTCGGAGCACATGACTATCCTACGAGTGGTGTTTTTGAGGTTGAACCTCGCCAATGCCCAGGGTTCAAGTTCAGAAGGTCGATATTGATCGGGACAACAGGCTTGGACCCTACTCAGGTTCGAGAGTTTATGGAGCGCCATTCTTTGAGCTACAACGGGGATACATATCACCTGATTGTCAAGAACTGCAACCATTTCTGCAGGGATATTTGTCGCAGGCTCACCGGAAAATCAATTCCTAAATGGGTGAACCGACTGGCAAGAATTGGTTCAGTATGCAACTGCATACTTCCGGAATCTCTGAAGATTTCCGATGTACAACACGACCCTAACTGCCAACCGTACGACAGCGACAAGAGGAGCTTGAGAAGCGCCTTCAGCTGCGTGTCGTCTATCTCAATGCGGCAGAAGCAGTTATCTTCATCTTCGTTATTTCTACAGTCGCCCCTGAAAGGTTGCTTGCCGCCATGGGAACTGCGAAGATCGTTGAATGGTTCGTTGAAAGAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

248

Amino Acids

28.06

Weight (kDa)

9.51

Isoelectric Point (pI)

50.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 42 - 177 8.9e-51 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 65
AccIII TCCGGA 1 cut(s) 523
AciI CCGC 4 cut(s) 63, 65, 634, 695
AclWI GGATC 1 cut(s) 24
AcsI RAATTY 2 cut(s) 5, 189
AcuI CTGAAG 2 cut(s) 554, 592
AfaI GTAC 2 cut(s) 549, 575
AfeI AGCGCT 1 cut(s) 106
AfiI CCNNNNNNNGG 4 cut(s) 161, 257, 346, 683
AgsI TTSAA 9 cut(s) 11, 16, 47, 52, 209, 275, 298, 721, 733
AjnI CCWGG 1 cut(s) 289
AleI CACNNNNGTG 1 cut(s) 201
AluBI AGCT 3 cut(s) 381, 594, 612
AluI AGCT 3 cut(s) 381, 594, 612
Alw21I GWGCWC 1 cut(s) 241
AlwI GGATC 1 cut(s) 24
Aor13HI TCCGGA 1 cut(s) 523
Aor51HI AGCGCT 1 cut(s) 106
ApeKI GCWGC 1 cut(s) 612
ApoI RAATTY 2 cut(s) 5, 189
Asp700I GAANNNNTTC 1 cut(s) 499
AspLEI GCGC 3 cut(s) 107, 369, 605
AspS9I GGNCC 1 cut(s) 337
AsuHPI GGTGA 3 cut(s) 392, 445, 490
AvaII GGWCC 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 241
BbvI GCAGC 1 cut(s) 599
BccI CCATC 1 cut(s) 18
BciT130I CCWGG 1 cut(s) 291
BciVI GTATCC 1 cut(s) 385
BfmI CTRYAG 2 cut(s) 430, 668
BfoI RGCGCY 3 cut(s) 108, 370, 606
BfuI GTATCC 1 cut(s) 385
BisI GCNGC 3 cut(s) 613, 635, 695
BlsI GCNGC 3 cut(s) 614, 636, 696
Bme1390I CCNGG 1 cut(s) 291
Bme18I GGWCC 1 cut(s) 337
BmgT120I GGNCC 1 cut(s) 337
BmiI GGNNCC 1 cut(s) 339
BmrFI CCNGG 1 cut(s) 291
BpuEI CTTGAG 1 cut(s) 616
BsaBI GATNNNNATC 3 cut(s) 28, 317, 396
BsaJI CCNNGG 4 cut(s) 63, 289, 290, 698
BsaWI WCCGGW 2 cut(s) 455, 523
Bsc4I CCNNNNNNNGG 4 cut(s) 161, 257, 346, 683
Bse1I ACTGG 2 cut(s) 125, 492
Bse8I GATNNNNATC 3 cut(s) 28, 317, 396
BseAI TCCGGA 1 cut(s) 523
BseBI CCWGG 1 cut(s) 291
BseDI CCNNGG 4 cut(s) 63, 289, 290, 698
BseJI GATNNNNATC 3 cut(s) 28, 317, 396
BseLI CCNNNNNNNGG 4 cut(s) 161, 257, 346, 683
BseMII CTCAG 1 cut(s) 359
BseNI ACTGG 2 cut(s) 125, 492
BseRI GAGGAG 1 cut(s) 604
BseXI GCAGC 1 cut(s) 599
Bsh1236I CGCG 1 cut(s) 65
BsiHKAI GWGCWC 1 cut(s) 241
BsiSI CCGG 2 cut(s) 456, 524
BsiWI CGTACG 1 cut(s) 573
BslFI GGGAC 1 cut(s) 337
BslI CCNNNNNNNGG 4 cut(s) 161, 257, 346, 683
BsmFI GGGAC 1 cut(s) 337
BsmI GAATGC 1 cut(s) 230
Bsp1286I GDGCHC 1 cut(s) 241
Bsp13I TCCGGA 1 cut(s) 523
Bsp1407I TGTACA 1 cut(s) 547
Bsp143I GATC 4 cut(s) 29, 145, 318, 713
Bsp19I CCATGG 1 cut(s) 698
BspACI CCGC 4 cut(s) 63, 65, 634, 695
BspCNI CTCAG 1 cut(s) 358
BspEI TCCGGA 1 cut(s) 523
BspFNI CGCG 1 cut(s) 65
BspLI GGNNCC 1 cut(s) 339
BspMAI CTGCAG 1 cut(s) 434
BspPI GGATC 1 cut(s) 24
BsrGI TGTACA 1 cut(s) 547
BsrI ACTGG 2 cut(s) 125, 492
BssECI CCNNGG 4 cut(s) 63, 289, 290, 698
BssMI GATC 4 cut(s) 29, 145, 318, 713
BssT1I CCWWGG 1 cut(s) 698
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 2 cut(s) 573, 672
Bst6I CTCTTC 1 cut(s) 14
BstAUI TGTACA 1 cut(s) 547
BstC8I GCNNGC 3 cut(s) 181, 449, 692
BstDEI CTNAG 1 cut(s) 345
BstDSI CCRYGG 2 cut(s) 63, 698
BstFNI CGCG 1 cut(s) 65
BstH2I RGCGCY 3 cut(s) 108, 370, 606
BstHHI GCGC 3 cut(s) 107, 369, 605
BstKTI GATC 4 cut(s) 32, 148, 321, 716
BstMBI GATC 4 cut(s) 29, 145, 318, 713
BstMWI GCNNNNNNNGC 4 cut(s) 236, 600, 609, 640
BstNI CCWGG 1 cut(s) 291
BstSCI CCNGG 1 cut(s) 289
BstSFI CTRYAG 2 cut(s) 430, 668
BstUI CGCG 1 cut(s) 65
BstV1I GCAGC 1 cut(s) 599
BstX2I RGATCY 1 cut(s) 29
BstYI RGATCY 1 cut(s) 29
BsuI GTATCC 1 cut(s) 385
BtgI CCRYGG 2 cut(s) 63, 698
Cac8I GCNNGC 3 cut(s) 181, 449, 692
CfoI GCGC 3 cut(s) 107, 369, 605
Cfr13I GGNCC 1 cut(s) 337
Cfr42I CCGCGG 1 cut(s) 66
Csp6I GTAC 2 cut(s) 548, 574
CviAII CATG 4 cut(s) 157, 215, 242, 699
CviJI RGCY 8 cut(s) 165, 179, 183, 333, 381, 451, 594, 612
CviKI_1 RGCY 8 cut(s) 165, 179, 183, 333, 381, 451, 594, 612
CviQI GTAC 2 cut(s) 548, 574
DdeI CTNAG 1 cut(s) 345
DpnI GATC 4 cut(s) 31, 147, 320, 715
DpnII GATC 4 cut(s) 29, 145, 318, 713
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
Eco130I CCWWGG 1 cut(s) 698
Eco47I GGWCC 1 cut(s) 337
Eco47III AGCGCT 1 cut(s) 106
Eco57I CTGAAG 2 cut(s) 554, 592
EcoRII CCWGG 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 698
EcoT22I ATGCAT 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 698
FaeI CATG 4 cut(s) 160, 218, 245, 702
FalI AAGNNNNNCTT 2 cut(s) 578, 610
FaqI GGGAC 1 cut(s) 337
FatI CATG 4 cut(s) 156, 214, 241, 698
FauI CCCGC 1 cut(s) 58
Fnu4HI GCNGC 3 cut(s) 613, 635, 695
Fsp4HI GCNGC 3 cut(s) 613, 635, 695
GlaI GCGC 3 cut(s) 106, 368, 604
GluI GCNGC 3 cut(s) 613, 635, 695
HaeII RGCGCY 3 cut(s) 108, 370, 606
HapII CCGG 2 cut(s) 456, 524
HhaI GCGC 3 cut(s) 107, 369, 605
Hin1II CATG 4 cut(s) 160, 218, 245, 702
Hin6I GCGC 3 cut(s) 105, 367, 603
HinP1I GCGC 3 cut(s) 105, 367, 603
HinfI GANTC 3 cut(s) 110, 151, 527
HpaII CCGG 2 cut(s) 456, 524
HphI GGTGA 3 cut(s) 392, 445, 490
Hpy166II GTNNAC 1 cut(s) 481
Hpy188I TCNGA 6 cut(s) 100, 150, 236, 305, 534, 544
Hpy188III TCNNGA 5 cut(s) 114, 322, 353, 412, 524
Hpy8I GTNNAC 1 cut(s) 481
HpyAV CCTTC 3 cut(s) 53, 300, 616
HpyCH4III ACNGT 2 cut(s) 573, 672
HpyCH4V TGCA 5 cut(s) 230, 420, 432, 510, 516
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 600, 609, 640
HpyF3I CTNAG 1 cut(s) 345
Hsp92II CATG 4 cut(s) 160, 218, 245, 702
HspAI GCGC 3 cut(s) 105, 367, 603
Kpn2I TCCGGA 1 cut(s) 523
KspI CCGCGG 1 cut(s) 66
Kzo9I GATC 4 cut(s) 29, 145, 318, 713
LmnI GCTCC 3 cut(s) 236, 364, 591
Lsp1109I GCAGC 1 cut(s) 599
MalI GATC 4 cut(s) 31, 147, 320, 715
MboI GATC 4 cut(s) 29, 145, 318, 713
MboII GAAGA 7 cut(s) 28, 31, 64, 547, 642, 648, 723
MfeI CAATTG 1 cut(s) 47
MflI RGATCY 1 cut(s) 29
MhlI GDGCHC 1 cut(s) 241
MluCI AATT 6 cut(s) 5, 11, 47, 189, 465, 495
MlyI GAGTC 1 cut(s) 145
MnlI CCTC 3 cut(s) 262, 288, 582
Mph1103I ATGCAT 1 cut(s) 232
MroI TCCGGA 1 cut(s) 523
MroXI GAANNNNTTC 1 cut(s) 499
MslI CAYNNNNRTG 1 cut(s) 201
MspA1I CMGCKG 2 cut(s) 65, 612
MspI CCGG 2 cut(s) 456, 524
MspR9I CCNGG 1 cut(s) 291
MunI CAATTG 1 cut(s) 47
Mva1269I GAATGC 1 cut(s) 230
MvaI CCWGG 1 cut(s) 291
MvnI CGCG 1 cut(s) 65
MwoI GCNNNNNNNGC 4 cut(s) 236, 600, 609, 640
NcoI CCATGG 1 cut(s) 698
NdeII GATC 4 cut(s) 29, 145, 318, 713
NlaIII CATG 4 cut(s) 160, 218, 245, 702
NlaIV GGNNCC 1 cut(s) 339
NsiI ATGCAT 1 cut(s) 232
OliI CACNNNNGTG 1 cut(s) 201
PasI CCCWGGG 1 cut(s) 290
PctI GAATGC 1 cut(s) 230
PdmI GAANNNNTTC 1 cut(s) 499
PfeI GAWTC 2 cut(s) 110, 527
Pfl23II CGTACG 1 cut(s) 573
PkrI GCNGC 3 cut(s) 614, 636, 696
PleI GAGTC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 145
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspLI CGTACG 1 cut(s) 573
PspN4I GGNNCC 1 cut(s) 339
PspPI GGNCC 1 cut(s) 337
PstI CTGCAG 1 cut(s) 434
PsuI RGATCY 1 cut(s) 29
PvuII CAGCTG 1 cut(s) 612
RsaI GTAC 2 cut(s) 549, 575
RsaNI GTAC 2 cut(s) 548, 574
RseI CAYNNNNRTG 1 cut(s) 201
SacII CCGCGG 1 cut(s) 66
SatI GCNGC 3 cut(s) 613, 635, 695
Sau3AI GATC 4 cut(s) 29, 145, 318, 713
Sau96I GGNCC 1 cut(s) 337
SchI GAGTC 1 cut(s) 145
ScrFI CCNGG 1 cut(s) 291
SduI GDGCHC 1 cut(s) 241
SfcI CTRYAG 2 cut(s) 430, 668
Sfr303I CCGCGG 1 cut(s) 66
SgrBI CCGCGG 1 cut(s) 66
SinI GGWCC 1 cut(s) 337
SmiMI CAYNNNNRTG 1 cut(s) 201
SmlI CTYRAG 1 cut(s) 595
SmoI CTYRAG 1 cut(s) 595
Sse9I AATT 6 cut(s) 5, 11, 47, 189, 465, 495
SsiI CCGC 4 cut(s) 63, 65, 634, 695
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 1 cut(s) 698
TaaI ACNGT 2 cut(s) 573, 672
TaqI TCGA 2 cut(s) 311, 352
TasI AATT 6 cut(s) 5, 11, 47, 189, 465, 495
TatI WGTACW 1 cut(s) 547
TauI GCSGC 2 cut(s) 637, 697
TfiI GAWTC 2 cut(s) 110, 527
TseI GCWGC 1 cut(s) 612
TspDTI ATGAA 4 cut(s) 17, 173, 203, 642
TspGWI ACGGA 1 cut(s) 22
VpaK11BI GGWCC 1 cut(s) 337
XapI RAATTY 2 cut(s) 5, 189
XmnI GAANNNNTTC 1 cut(s) 499
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.