FvH4_2g40100

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002011
Physical Location & Seq
Forward (+)
28652812 .. 28656320
3509 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g40100.t4

Sequence Viewer

Length: 741 bp
ATGAAATTTGGATTAAAGAAAAGATGGAAATCCATTGTCCCACTTCAGTTGAAAAATAAATCAGCCAATCGCTTTTGCTTATTTCCCAAATCGAAGTCGGGTTTTGGTCCAGGCAAAGCACCTGTATATCTCAATGTATATGACCTGACAACCATGAATGGCTATGTCTATTGGGCAGGCTTTGGAATTTTTCACTCTGGTGTTGAAGTTCATGGTGTAGAATATGCATTTGGAGCACATGACTACCCTACCAGTGGTGTTTTTGAGGTTGAACCTCGGCAATGCCCAGGATTCAAGTTCAGGAAATCTATATTTATTGGGACCACAAGCTTGGACCCTATTCAGGTCAGGGAGTTTATGGAGCGCCAGTCATTGAGCTACAATGGTGATACATATCACCTGATTGTCAAGAATTGCAACCATTTCTGCAGAGATATCTGCTACAAGCTAACCGGGAAATCAATTCCAAAGTGGGTAAATCGACTGGCAAGAATAGGTTCAATATGCAACTGCATACTTCCTGAAGCGCTAAAAATTTCTGCTGTACAACACGACCCTAACAACCAACCATATGATAGCGAGAAAAGGAGTTTGAGAAGTGCCTTCAGTTGCCTGTCTTCTATCTCAATGCGGCAAAAGCAATTGTCATCATCTTCATTGTTTCTACAGTCACCCTTGAAAGGCTGCTTGCCACCATGGGAATTAAGAAGGTCAAACAATGGGTCCTTGAAGGAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

247

Amino Acids

27.95

Weight (kDa)

9.67

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 41 - 176 1e-49 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 631
AcsI RAATTY 3 cut(s) 5, 186, 534
AcuI CTGAAG 3 cut(s) 29, 543, 589
AfaI GTAC 1 cut(s) 546
AfeI AGCGCT 1 cut(s) 528
AfiI CCNNNNNNNGG 3 cut(s) 254, 343, 680
AgsI TTSAA 7 cut(s) 52, 206, 272, 295, 501, 679, 730
AjnI CCWGG 2 cut(s) 109, 286
AjuI GAANNNNNNNTTGG 2 cut(s) 213, 245
AleI CACNNNNGTG 1 cut(s) 198
AluBI AGCT 3 cut(s) 330, 378, 448
AluI AGCT 3 cut(s) 330, 378, 448
Alw21I GWGCWC 1 cut(s) 238
Aor51HI AGCGCT 1 cut(s) 528
ApeKI GCWGC 1 cut(s) 684
ApoI RAATTY 3 cut(s) 5, 186, 534
ArsI GACNNNNNNTTYG 2 cut(s) 707, 739
Asp700I GAANNNNTTC 1 cut(s) 496
AspLEI GCGC 2 cut(s) 366, 529
AspS9I GGNCC 4 cut(s) 107, 321, 334, 723
AsuC2I CCSGG 1 cut(s) 454
AsuHPI GGTGA 3 cut(s) 389, 398, 663
AvaII GGWCC 4 cut(s) 107, 321, 334, 723
BbsI GAAGAC 1 cut(s) 609
Bbv12I GWGCWC 1 cut(s) 238
BbvI GCAGC 1 cut(s) 671
BccI CCATC 1 cut(s) 18
BciT130I CCWGG 2 cut(s) 111, 288
BcnI CCSGG 1 cut(s) 454
BfmI CTRYAG 2 cut(s) 427, 665
BfoI RGCGCY 2 cut(s) 367, 530
BisI GCNGC 2 cut(s) 632, 685
BlsI GCNGC 2 cut(s) 633, 686
Bme1390I CCNGG 3 cut(s) 111, 288, 454
Bme18I GGWCC 4 cut(s) 107, 321, 334, 723
BmgT120I GGNCC 4 cut(s) 107, 321, 334, 723
BmiI GGNNCC 3 cut(s) 322, 336, 724
BmrFI CCNGG 3 cut(s) 111, 288, 454
BpiI GAAGAC 1 cut(s) 609
BpuMI CCSGG 1 cut(s) 454
BsaBI GATNNNNATC 2 cut(s) 28, 393
BsaJI CCNNGG 3 cut(s) 275, 286, 695
BsaXI ACNNNNNCTCC 2 cut(s) 353, 383
Bsc4I CCNNNNNNNGG 3 cut(s) 254, 343, 680
Bse1I ACTGG 3 cut(s) 252, 367, 489
Bse3DI GCAATG 1 cut(s) 287
Bse8I GATNNNNATC 2 cut(s) 28, 393
BseBI CCWGG 2 cut(s) 111, 288
BseDI CCNNGG 3 cut(s) 275, 286, 695
BseJI GATNNNNATC 2 cut(s) 28, 393
BseLI CCNNNNNNNGG 3 cut(s) 254, 343, 680
BseMI GCAATG 1 cut(s) 287
BseNI ACTGG 3 cut(s) 252, 367, 489
BseXI GCAGC 1 cut(s) 671
BsiHKAI GWGCWC 1 cut(s) 238
BsiSI CCGG 1 cut(s) 453
BslFI GGGAC 2 cut(s) 23, 334
BslI CCNNNNNNNGG 3 cut(s) 254, 343, 680
BsmFI GGGAC 2 cut(s) 23, 334
Bsp1286I GDGCHC 1 cut(s) 238
Bsp1407I TGTACA 1 cut(s) 544
Bsp19I CCATGG 1 cut(s) 695
BspACI CCGC 1 cut(s) 631
BspLI GGNNCC 3 cut(s) 322, 336, 724
BspMAI CTGCAG 1 cut(s) 431
BsrDI GCAATG 1 cut(s) 287
BsrGI TGTACA 1 cut(s) 544
BsrI ACTGG 3 cut(s) 252, 367, 489
BssECI CCNNGG 3 cut(s) 275, 286, 695
BssT1I CCWWGG 1 cut(s) 695
Bst2UI CCWGG 2 cut(s) 111, 288
Bst4CI ACNGT 1 cut(s) 669
BstAUI TGTACA 1 cut(s) 544
BstC8I GCNNGC 2 cut(s) 178, 689
BstDSI CCRYGG 1 cut(s) 695
BstH2I RGCGCY 2 cut(s) 367, 530
BstHHI GCGC 2 cut(s) 366, 529
BstMWI GCNNNNNNNGC 2 cut(s) 233, 637
BstNI CCWGG 2 cut(s) 111, 288
BstSCI CCNGG 3 cut(s) 109, 286, 452
BstSFI CTRYAG 2 cut(s) 427, 665
BstV1I GCAGC 1 cut(s) 671
BstV2I GAAGAC 1 cut(s) 609
BstXI CCANNNNNNTGG 1 cut(s) 331
BtgI CCRYGG 1 cut(s) 695
BtsIMutI CAGTG 1 cut(s) 259
Cac8I GCNNGC 2 cut(s) 178, 689
CfoI GCGC 2 cut(s) 366, 529
Cfr13I GGNCC 4 cut(s) 107, 321, 334, 723
Csp6I GTAC 1 cut(s) 545
CspCI CAANNNNNGTGG 2 cut(s) 30, 65
CviAII CATG 4 cut(s) 154, 212, 239, 696
CviJI RGCY 7 cut(s) 65, 162, 180, 330, 378, 448, 684
CviKI_1 RGCY 7 cut(s) 65, 162, 180, 330, 378, 448, 684
CviQI GTAC 1 cut(s) 545
Eco130I CCWWGG 1 cut(s) 695
Eco32I GATATC 1 cut(s) 436
Eco47I GGWCC 4 cut(s) 107, 321, 334, 723
Eco47III AGCGCT 1 cut(s) 528
Eco57I CTGAAG 3 cut(s) 29, 543, 589
EcoO109I RGGNCCY 1 cut(s) 723
EcoRII CCWGG 2 cut(s) 109, 286
EcoRV GATATC 1 cut(s) 436
EcoT14I CCWWGG 1 cut(s) 695
EcoT22I ATGCAT 1 cut(s) 229
ErhI CCWWGG 1 cut(s) 695
FaeI CATG 4 cut(s) 157, 215, 242, 699
FaqI GGGAC 2 cut(s) 23, 334
FatI CATG 4 cut(s) 153, 211, 238, 695
FauNDI CATATG 1 cut(s) 571
Fnu4HI GCNGC 2 cut(s) 632, 685
Fsp4HI GCNGC 2 cut(s) 632, 685
GlaI GCGC 2 cut(s) 365, 528
GluI GCNGC 2 cut(s) 632, 685
HaeII RGCGCY 2 cut(s) 367, 530
HapII CCGG 1 cut(s) 453
HhaI GCGC 2 cut(s) 366, 529
Hin1II CATG 4 cut(s) 157, 215, 242, 699
Hin6I GCGC 2 cut(s) 364, 527
HinP1I GCGC 2 cut(s) 364, 527
HindIII AAGCTT 1 cut(s) 328
HinfI GANTC 1 cut(s) 291
HpaII CCGG 1 cut(s) 453
HphI GGTGA 3 cut(s) 389, 398, 663
Hpy188III TCNNGA 3 cut(s) 301, 409, 521
HpyAV CCTTC 3 cut(s) 613, 702, 724
HpyCH4III ACNGT 1 cut(s) 669
HpyCH4V TGCA 5 cut(s) 227, 417, 429, 507, 513
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 637
Hsp92II CATG 4 cut(s) 157, 215, 242, 699
HspAI GCGC 2 cut(s) 364, 527
LmnI GCTCC 2 cut(s) 233, 361
Lsp1109I GCAGC 1 cut(s) 671
MaeIII GTNAC 1 cut(s) 669
MboII GAAGA 2 cut(s) 609, 645
MfeI CAATTG 1 cut(s) 641
MhlI GDGCHC 1 cut(s) 238
MluCI AATT 7 cut(s) 5, 186, 412, 462, 534, 641, 701
MnlI CCTC 2 cut(s) 259, 285
Mph1103I ATGCAT 1 cut(s) 229
MroXI GAANNNNTTC 1 cut(s) 496
MseI TTAA 2 cut(s) 14, 704
MslI CAYNNNNRTG 1 cut(s) 198
MspI CCGG 1 cut(s) 453
MspR9I CCNGG 3 cut(s) 111, 288, 454
MunI CAATTG 1 cut(s) 641
MvaI CCWGG 2 cut(s) 111, 288
MwoI GCNNNNNNNGC 2 cut(s) 233, 637
NciI CCSGG 1 cut(s) 454
NcoI CCATGG 1 cut(s) 695
NdeI CATATG 1 cut(s) 571
NlaIII CATG 4 cut(s) 157, 215, 242, 699
NlaIV GGNNCC 3 cut(s) 322, 336, 724
NmeAIII GCCGAG 1 cut(s) 256
NmuCI GTSAC 1 cut(s) 669
NsiI ATGCAT 1 cut(s) 229
OliI CACNNNNGTG 1 cut(s) 198
PdmI GAANNNNTTC 1 cut(s) 496
PfeI GAWTC 1 cut(s) 291
PkrI GCNGC 2 cut(s) 633, 686
PpuMI RGGWCCY 1 cut(s) 723
Psp5II RGGWCCY 1 cut(s) 723
Psp6I CCWGG 2 cut(s) 109, 286
PspGI CCWGG 2 cut(s) 109, 286
PspN4I GGNNCC 3 cut(s) 322, 336, 724
PspPI GGNCC 4 cut(s) 107, 321, 334, 723
PspPPI RGGWCCY 1 cut(s) 723
PstI CTGCAG 1 cut(s) 431
RsaI GTAC 1 cut(s) 546
RsaNI GTAC 1 cut(s) 545
RseI CAYNNNNRTG 1 cut(s) 198
SaqAI TTAA 2 cut(s) 14, 704
SatI GCNGC 2 cut(s) 632, 685
Sau96I GGNCC 4 cut(s) 107, 321, 334, 723
ScrFI CCNGG 3 cut(s) 111, 288, 454
SduI GDGCHC 1 cut(s) 238
SfcI CTRYAG 2 cut(s) 427, 665
SinI GGWCC 4 cut(s) 107, 321, 334, 723
SmiMI CAYNNNNRTG 1 cut(s) 198
Sse9I AATT 7 cut(s) 5, 186, 412, 462, 534, 641, 701
SsiI CCGC 1 cut(s) 631
StyD4I CCNGG 3 cut(s) 109, 286, 452
StyI CCWWGG 1 cut(s) 695
TaaI ACNGT 1 cut(s) 669
TaqI TCGA 2 cut(s) 92, 481
TasI AATT 7 cut(s) 5, 186, 412, 462, 534, 641, 701
TatI WGTACW 1 cut(s) 544
TauI GCSGC 1 cut(s) 634
TfiI GAWTC 1 cut(s) 291
Tru1I TTAA 2 cut(s) 14, 704
Tru9I TTAA 2 cut(s) 14, 704
TscAI CASTG 1 cut(s) 259
TseFI GTSAC 1 cut(s) 669
TseI GCWGC 1 cut(s) 684
Tsp45I GTSAC 1 cut(s) 669
TspDTI ATGAA 4 cut(s) 17, 170, 200, 645
TspRI CASTG 1 cut(s) 259
VpaK11BI GGWCC 4 cut(s) 107, 321, 334, 723
XapI RAATTY 3 cut(s) 5, 186, 534
XmnI GAANNNNTTC 1 cut(s) 496
Zsp2I ATGCAT 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.