pycom08g02570

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002011
Physical Location & Seq
Reverse (-)
1888683 .. 1889975
1293 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g02570.3

Sequence Viewer

Length: 744 bp
ATGAAATTTGAATTGAAGAAGAGATGGAGATCCATGGCCCCCCTTCAATTGAAAAACAAATCCGCGGGGCGGTTTTGTTTATTTCCCAAATCAAAATCGGATAGCGCTGAATCAGGAAAAGCACGAGTTTATCTCAATGTATATGACTTGACTCCCATGAATGGCTATGTCTATTGGGCTGGCTTTGGAATTTTTCACTCTGGTGTTGAAGTTCATGGTATAGAATATGCATTCGGAGCACATGACTATCCTACAAGTGGCGTTTTTGAGGTTGAACCTCGCCAATGCCCAGGGTTCAAGTTCAGAAAGTCGATATTAATCGGGACAACAGGCTTGGACCCTACTCAGGTTCGAGAGTTTATGGAGCGCCATTCTTTGAGCTACAACGGTGATACATATCACCTGATTGTCAAGAACTGCAACCATTTCTGCAGGGATATTTGTCGCAGGCTCACCGGAAAATCAATTCCTAAATGGGTGAACCGACTGGCAAGAATTGGTTCAGTATGCAACTGCATACTTCCGGAATCTCTGAAGATTTCCGCTGTACAACACGACCCTAACTGCCAACCATACGACAGCGACAAGAGGAGCTTGAGAAGCACCTTCGGTTGCCTGTCGTCTATCTCAATGCGGCAGAAGCAGTTATCTTCATCTTCGTTATTTCTACAGTCGCCCCTGAAAGGTTGCTTGCCGCCATGGGAACTGCGAAGATCGTTGAATGGTTCATTGAAAGAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

248

Amino Acids

28.11

Weight (kDa)

9.61

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 65
AccIII TCCGGA 1 cut(s) 523
AciI CCGC 6 cut(s) 63, 65, 70, 543, 634, 695
AclWI GGATC 1 cut(s) 24
AcsI RAATTY 2 cut(s) 5, 189
AcuI CTGAAG 1 cut(s) 554
AfaI GTAC 1 cut(s) 549
AfeI AGCGCT 1 cut(s) 106
AfiI CCNNNNNNNGG 5 cut(s) 69, 161, 257, 346, 683
AgsI TTSAA 9 cut(s) 11, 16, 47, 52, 209, 275, 298, 721, 733
AjnI CCWGG 1 cut(s) 289
AleI CACNNNNGTG 1 cut(s) 201
AluBI AGCT 2 cut(s) 381, 594
AluI AGCT 2 cut(s) 381, 594
Alw21I GWGCWC 1 cut(s) 241
AlwI GGATC 1 cut(s) 24
Aor13HI TCCGGA 1 cut(s) 523
Aor51HI AGCGCT 1 cut(s) 106
AoxI GGCC 1 cut(s) 36
ApoI RAATTY 2 cut(s) 5, 189
AseI ATTAAT 1 cut(s) 317
Asp700I GAANNNNTTC 1 cut(s) 499
AspLEI GCGC 2 cut(s) 107, 369
AspS9I GGNCC 2 cut(s) 37, 337
AsuHPI GGTGA 4 cut(s) 392, 401, 445, 490
AvaII GGWCC 1 cut(s) 337
BauI CACGAG 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 241
BccI CCATC 1 cut(s) 18
BciT130I CCWGG 1 cut(s) 291
BfmI CTRYAG 2 cut(s) 430, 668
BfoI RGCGCY 2 cut(s) 108, 370
BisI GCNGC 2 cut(s) 635, 695
BlsI GCNGC 2 cut(s) 636, 696
Bme1390I CCNGG 1 cut(s) 291
Bme18I GGWCC 1 cut(s) 337
BmgT120I GGNCC 2 cut(s) 37, 337
BmiI GGNNCC 2 cut(s) 39, 339
BmrFI CCNGG 1 cut(s) 291
BplI GAGNNNNNCTC 2 cut(s) 117, 149
BpuEI CTTGAG 1 cut(s) 616
BsaBI GATNNNNATC 3 cut(s) 28, 317, 396
BsaJI CCNNGG 5 cut(s) 33, 63, 289, 290, 698
BsaWI WCCGGW 2 cut(s) 455, 523
Bsc4I CCNNNNNNNGG 5 cut(s) 69, 161, 257, 346, 683
Bse1I ACTGG 1 cut(s) 492
Bse8I GATNNNNATC 3 cut(s) 28, 317, 396
BseAI TCCGGA 1 cut(s) 523
BseBI CCWGG 1 cut(s) 291
BseDI CCNNGG 5 cut(s) 33, 63, 289, 290, 698
BseJI GATNNNNATC 3 cut(s) 28, 317, 396
BseLI CCNNNNNNNGG 5 cut(s) 69, 161, 257, 346, 683
BseMII CTCAG 1 cut(s) 359
BseNI ACTGG 1 cut(s) 492
BseRI GAGGAG 1 cut(s) 604
Bsh1236I CGCG 1 cut(s) 65
BshFI GGCC 1 cut(s) 38
BsiHKAI GWGCWC 1 cut(s) 241
BsiSI CCGG 2 cut(s) 456, 524
BslFI GGGAC 1 cut(s) 337
BslI CCNNNNNNNGG 5 cut(s) 69, 161, 257, 346, 683
BsmFI GGGAC 1 cut(s) 337
BsmI GAATGC 1 cut(s) 230
BsnI GGCC 1 cut(s) 38
Bsp1286I GDGCHC 1 cut(s) 241
Bsp13I TCCGGA 1 cut(s) 523
Bsp1407I TGTACA 1 cut(s) 547
Bsp143I GATC 2 cut(s) 29, 713
Bsp19I CCATGG 2 cut(s) 33, 698
BspACI CCGC 6 cut(s) 63, 65, 70, 543, 634, 695
BspANI GGCC 1 cut(s) 38
BspCNI CTCAG 1 cut(s) 358
BspEI TCCGGA 1 cut(s) 523
BspFNI CGCG 1 cut(s) 65
BspLI GGNNCC 2 cut(s) 39, 339
BspMAI CTGCAG 1 cut(s) 434
BspPI GGATC 1 cut(s) 24
BsrGI TGTACA 1 cut(s) 547
BsrI ACTGG 1 cut(s) 492
BssECI CCNNGG 5 cut(s) 33, 63, 289, 290, 698
BssMI GATC 2 cut(s) 29, 713
BssSI CACGAG 1 cut(s) 123
BssT1I CCWWGG 2 cut(s) 33, 698
Bst2BI CACGAG 1 cut(s) 123
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 2 cut(s) 389, 672
Bst6I CTCTTC 1 cut(s) 14
BstAUI TGTACA 1 cut(s) 547
BstC8I GCNNGC 3 cut(s) 181, 449, 692
BstDEI CTNAG 1 cut(s) 345
BstDSI CCRYGG 3 cut(s) 33, 63, 698
BstFNI CGCG 1 cut(s) 65
BstH2I RGCGCY 2 cut(s) 108, 370
BstHHI GCGC 2 cut(s) 107, 369
BstKTI GATC 2 cut(s) 32, 716
BstMBI GATC 2 cut(s) 29, 713
BstMWI GCNNNNNNNGC 3 cut(s) 236, 600, 640
BstNI CCWGG 1 cut(s) 291
BstSCI CCNGG 1 cut(s) 289
BstSFI CTRYAG 2 cut(s) 430, 668
BstUI CGCG 1 cut(s) 65
BstX2I RGATCY 1 cut(s) 29
BstYI RGATCY 1 cut(s) 29
BsuRI GGCC 1 cut(s) 38
BtgI CCRYGG 3 cut(s) 33, 63, 698
Cac8I GCNNGC 3 cut(s) 181, 449, 692
CfoI GCGC 2 cut(s) 107, 369
Cfr13I GGNCC 2 cut(s) 37, 337
Cfr42I CCGCGG 1 cut(s) 66
Csp6I GTAC 1 cut(s) 548
CviAII CATG 5 cut(s) 34, 157, 215, 242, 699
CviJI RGCY 8 cut(s) 38, 165, 179, 183, 333, 381, 451, 594
CviKI_1 RGCY 8 cut(s) 38, 165, 179, 183, 333, 381, 451, 594
CviQI GTAC 1 cut(s) 548
DdeI CTNAG 1 cut(s) 345
DpnI GATC 2 cut(s) 31, 715
DpnII GATC 2 cut(s) 29, 713
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
Eco130I CCWWGG 2 cut(s) 33, 698
Eco47I GGWCC 1 cut(s) 337
Eco47III AGCGCT 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 554
EcoRII CCWGG 1 cut(s) 289
EcoT14I CCWWGG 2 cut(s) 33, 698
EcoT22I ATGCAT 1 cut(s) 232
ErhI CCWWGG 2 cut(s) 33, 698
FaeI CATG 5 cut(s) 37, 160, 218, 245, 702
FalI AAGNNNNNCTT 2 cut(s) 578, 610
FaqI GGGAC 1 cut(s) 337
FatI CATG 5 cut(s) 33, 156, 214, 241, 698
FauI CCCGC 1 cut(s) 58
Fnu4HI GCNGC 2 cut(s) 635, 695
Fsp4HI GCNGC 2 cut(s) 635, 695
GlaI GCGC 2 cut(s) 106, 368
GluI GCNGC 2 cut(s) 635, 695
HaeII RGCGCY 2 cut(s) 108, 370
HaeIII GGCC 1 cut(s) 38
HapII CCGG 2 cut(s) 456, 524
HhaI GCGC 2 cut(s) 107, 369
Hin1II CATG 5 cut(s) 37, 160, 218, 245, 702
Hin6I GCGC 2 cut(s) 105, 367
HinP1I GCGC 2 cut(s) 105, 367
HinfI GANTC 3 cut(s) 110, 151, 527
HpaII CCGG 2 cut(s) 456, 524
HphI GGTGA 4 cut(s) 392, 401, 445, 490
Hpy166II GTNNAC 1 cut(s) 481
Hpy188I TCNGA 4 cut(s) 100, 236, 305, 534
Hpy188III TCNNGA 5 cut(s) 114, 322, 353, 412, 524
Hpy8I GTNNAC 1 cut(s) 481
HpyAV CCTTC 2 cut(s) 53, 616
HpyCH4III ACNGT 2 cut(s) 389, 672
HpyCH4V TGCA 5 cut(s) 230, 420, 432, 510, 516
HpyF10VI GCNNNNNNNGC 3 cut(s) 236, 600, 640
HpyF3I CTNAG 1 cut(s) 345
Hsp92II CATG 5 cut(s) 37, 160, 218, 245, 702
HspAI GCGC 2 cut(s) 105, 367
Kpn2I TCCGGA 1 cut(s) 523
KspI CCGCGG 1 cut(s) 66
Kzo9I GATC 2 cut(s) 29, 713
LmnI GCTCC 3 cut(s) 236, 364, 591
MalI GATC 2 cut(s) 31, 715
MboI GATC 2 cut(s) 29, 713
MboII GAAGA 6 cut(s) 28, 31, 547, 642, 648, 723
MfeI CAATTG 1 cut(s) 47
MflI RGATCY 1 cut(s) 29
MhlI GDGCHC 1 cut(s) 241
MluCI AATT 6 cut(s) 5, 11, 47, 189, 465, 495
MlyI GAGTC 1 cut(s) 145
MnlI CCTC 3 cut(s) 262, 288, 582
Mph1103I ATGCAT 1 cut(s) 232
MroI TCCGGA 1 cut(s) 523
MroXI GAANNNNTTC 1 cut(s) 499
MseI TTAA 1 cut(s) 317
MslI CAYNNNNRTG 1 cut(s) 201
MspA1I CMGCKG 2 cut(s) 65, 545
MspI CCGG 2 cut(s) 456, 524
MspR9I CCNGG 1 cut(s) 291
MunI CAATTG 1 cut(s) 47
Mva1269I GAATGC 1 cut(s) 230
MvaI CCWGG 1 cut(s) 291
MvnI CGCG 1 cut(s) 65
MwoI GCNNNNNNNGC 3 cut(s) 236, 600, 640
NcoI CCATGG 2 cut(s) 33, 698
NdeII GATC 2 cut(s) 29, 713
NlaIII CATG 5 cut(s) 37, 160, 218, 245, 702
NlaIV GGNNCC 2 cut(s) 39, 339
NsiI ATGCAT 1 cut(s) 232
OliI CACNNNNGTG 1 cut(s) 201
PasI CCCWGGG 1 cut(s) 290
PctI GAATGC 1 cut(s) 230
PdmI GAANNNNTTC 1 cut(s) 499
PfeI GAWTC 2 cut(s) 110, 527
PkrI GCNGC 2 cut(s) 636, 696
PleI GAGTC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 145
PshBI ATTAAT 1 cut(s) 317
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 2 cut(s) 39, 339
PspPI GGNCC 2 cut(s) 37, 337
PstI CTGCAG 1 cut(s) 434
PsuI RGATCY 1 cut(s) 29
RsaI GTAC 1 cut(s) 549
RsaNI GTAC 1 cut(s) 548
RseI CAYNNNNRTG 1 cut(s) 201
SacII CCGCGG 1 cut(s) 66
SaqAI TTAA 1 cut(s) 317
SatI GCNGC 2 cut(s) 635, 695
Sau3AI GATC 2 cut(s) 29, 713
Sau96I GGNCC 2 cut(s) 37, 337
SchI GAGTC 1 cut(s) 145
ScrFI CCNGG 1 cut(s) 291
SduI GDGCHC 1 cut(s) 241
SetI ASST 9 cut(s) 273, 280, 351, 383, 405, 596, 608, 688, 743
SfcI CTRYAG 2 cut(s) 430, 668
Sfr303I CCGCGG 1 cut(s) 66
SgrBI CCGCGG 1 cut(s) 66
SinI GGWCC 1 cut(s) 337
SmiMI CAYNNNNRTG 1 cut(s) 201
SmlI CTYRAG 1 cut(s) 595
SmoI CTYRAG 1 cut(s) 595
Sse9I AATT 6 cut(s) 5, 11, 47, 189, 465, 495
SsiI CCGC 6 cut(s) 63, 65, 70, 543, 634, 695
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 2 cut(s) 33, 698
TaaI ACNGT 2 cut(s) 389, 672
TaqI TCGA 2 cut(s) 311, 352
TasI AATT 6 cut(s) 5, 11, 47, 189, 465, 495
TatI WGTACW 1 cut(s) 547
TauI GCSGC 2 cut(s) 637, 697
TfiI GAWTC 2 cut(s) 110, 527
Tru1I TTAA 1 cut(s) 317
Tru9I TTAA 1 cut(s) 317
TspDTI ATGAA 5 cut(s) 17, 173, 203, 642, 717
VpaK11BI GGWCC 1 cut(s) 337
VspI ATTAAT 1 cut(s) 317
XapI RAATTY 2 cut(s) 5, 189
XmnI GAANNNNTTC 1 cut(s) 499
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.