MD05G1070300.v1.1

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002011
Physical Location & Seq
Forward (+)
13585476 .. 13588759
3284 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1070300.v1.1.491

Sequence Viewer

Length: 855 bp
ATGAAATCAGGACCAAAGGATGGCAGGCACTCTATTATGCCTCTTTGTTTGAGAGGCAAAGCAGCCTCACATTTTTGCATGTTTCCAAAGGTGAAGGCAGCAGACTACGTTAAAGGGAGCGCACCTGTCTATCTGAATGTATATGACTTGACACCGGCAAATGGCTATATCTATTGGGCAGGCCTTGGCATCTTTCACTCTGGCGTAGAAGTTCATGGGGTAGAATATGCCTTTGGAGCCCATGAGTACTCATCAAGCGGTGTTTTCGAGGTTGAACCTCGACAATGCCCTGGCTTCAAGTTTAGAAGGTCAATTTTCATTGGCATGACATGCTTGGATCCTTTCCAGGTTAGAGAGTTCATGGAGCGTCAATCTGCAATCTACAATGGTGACTCATATCACTTGATTGTCAAGAACTGCAACCATTTCTGTGAAGATATAGGTTATAAGCTGACTGGAAAGCGAATACCAAAATGGGTCAATCGACTTGCAAGAATAGGTTCTCTGTGCAACTGTATACTCCCAGACACCCTAAAGACGACCACTGTTCCACATGACCCCAATTTTGAAGGATGCGAAAGTGAAAAAAAGAGACTAAGAAGTGCCTTCACTTGCTTGTCATCAATGTCGATGCCACAGAGGGAAGTATCAATGTCGTCGTTGTTTCTACATTCTCACTATAAAGGCTGCCTACCACCATGGGAGTTTAAAAGGTCTAGAAATGGCTCATTGAAGGAAGGGATAGATGCTGCTGCTAGTTTTGTGGTTCAGGATGATCGGGGCTGTCCTATTCTTGCAGGAATCATAAATTTAAGGAATAATGCAGTATCAAGGCGGACTCTAGGAGTTGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

285

Amino Acids

31.76

Weight (kDa)

9.11

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 42 - 176 4.1e-47 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 447
AccB7I CCANNNNNTGG 1 cut(s) 20
AccI GTMKAC 1 cut(s) 517
AciI CCGC 2 cut(s) 258, 835
AclWI GGATC 2 cut(s) 332, 345
AcsI RAATTY 1 cut(s) 808
AfaI GTAC 1 cut(s) 248
AfiI CCNNNNNNNGG 2 cut(s) 20, 161
AgsI TTSAA 4 cut(s) 275, 298, 569, 733
AjnI CCWGG 2 cut(s) 289, 345
AjuI GAANNNNNNNTTGG 2 cut(s) 216, 248
AluBI AGCT 1 cut(s) 451
AluI AGCT 1 cut(s) 451
Alw26I GTCTC 1 cut(s) 586
AlwI GGATC 2 cut(s) 332, 345
AoxI GGCC 1 cut(s) 181
ApeKI GCWGC 5 cut(s) 62, 98, 687, 749, 752
ApoI RAATTY 1 cut(s) 808
ArsI GACNNNNNNTTYG 2 cut(s) 548, 580
Asp700I GAANNNNTTC 1 cut(s) 499
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 11
AsuHPI GGTGA 2 cut(s) 103, 401
AvaII GGWCC 1 cut(s) 11
BamHI GGATCC 1 cut(s) 337
BanII GRGCYC 1 cut(s) 241
BbvI GCAGC 5 cut(s) 74, 110, 674, 736, 739
BccI CCATC 1 cut(s) 14
BciT130I CCWGG 2 cut(s) 291, 347
BcoDI GTCTC 1 cut(s) 586
BfaI CTAG 3 cut(s) 717, 756, 842
BisI GCNGC 5 cut(s) 63, 99, 688, 750, 753
BlsI GCNGC 5 cut(s) 64, 100, 689, 751, 754
BmcAI AGTACT 1 cut(s) 248
Bme1390I CCNGG 2 cut(s) 291, 347
Bme18I GGWCC 1 cut(s) 11
BmgT120I GGNCC 1 cut(s) 11
BmiI GGNNCC 2 cut(s) 238, 339
BmrFI CCNGG 2 cut(s) 291, 347
BmsI GCATC 4 cut(s) 198, 563, 621, 736
BsaJI CCNNGG 3 cut(s) 184, 289, 698
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 161
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 1 cut(s) 460
BseBI CCWGG 2 cut(s) 291, 347
BseDI CCNNGG 3 cut(s) 184, 289, 698
BseGI GGATG 3 cut(s) 25, 578, 778
BseLI CCNNNNNNNGG 2 cut(s) 20, 161
BseNI ACTGG 1 cut(s) 460
BseXI GCAGC 5 cut(s) 74, 110, 674, 736, 739
BshFI GGCC 1 cut(s) 183
BsiSI CCGG 1 cut(s) 155
BslI CCNNNNNNNGG 2 cut(s) 20, 161
BsmAI GTCTC 1 cut(s) 586
BsnI GGCC 1 cut(s) 183
Bsp1286I GDGCHC 1 cut(s) 241
Bsp143I GATC 2 cut(s) 337, 775
Bsp19I CCATGG 1 cut(s) 698
BspACI CCGC 2 cut(s) 258, 835
BspANI GGCC 1 cut(s) 183
BspLI GGNNCC 2 cut(s) 238, 339
BspPI GGATC 2 cut(s) 332, 345
BsrFI RCCGGY 1 cut(s) 154
BsrI ACTGG 1 cut(s) 460
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 3 cut(s) 184, 289, 698
BssMI GATC 2 cut(s) 337, 775
BssNAI GTATAC 1 cut(s) 518
BssT1I CCWWGG 2 cut(s) 184, 698
Bst1107I GTATAC 1 cut(s) 518
Bst2UI CCWGG 2 cut(s) 291, 347
Bst4CI ACNGT 2 cut(s) 515, 547
BstAPI GCANNNNNTGC 1 cut(s) 330
BstC8I GCNNGC 2 cut(s) 26, 181
BstDEI CTNAG 1 cut(s) 596
BstDSI CCRYGG 1 cut(s) 698
BstF5I GGATG 3 cut(s) 25, 578, 778
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 2 cut(s) 340, 778
BstMAI GTCTC 1 cut(s) 586
BstMBI GATC 2 cut(s) 337, 775
BstMWI GCNNNNNNNGC 2 cut(s) 236, 330
BstNI CCWGG 2 cut(s) 291, 347
BstNSI RCATGY 2 cut(s) 82, 333
BstSCI CCNGG 2 cut(s) 289, 345
BstV1I GCAGC 5 cut(s) 74, 110, 674, 736, 739
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
BstZ17I GTATAC 1 cut(s) 518
BsuRI GGCC 1 cut(s) 183
BtgI CCRYGG 1 cut(s) 698
BtsCI GGATG 3 cut(s) 25, 578, 778
BtsIMutI CAGTG 1 cut(s) 543
Cac8I GCNNGC 2 cut(s) 26, 181
CfoI GCGC 1 cut(s) 122
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 1 cut(s) 11
CseI GACGC 1 cut(s) 356
Csp6I GTAC 1 cut(s) 247
CviAII CATG 8 cut(s) 79, 215, 242, 325, 330, 361, 554, 699
CviJI RGCY 9 cut(s) 65, 165, 183, 239, 294, 451, 687, 726, 783
CviKI_1 RGCY 9 cut(s) 65, 165, 183, 239, 294, 451, 687, 726, 783
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 1 cut(s) 596
DpnI GATC 2 cut(s) 339, 777
DpnII GATC 2 cut(s) 337, 775
DraI TTTAAA 1 cut(s) 709
EciI GGCGGA 1 cut(s) 850
Eco130I CCWWGG 2 cut(s) 184, 698
Eco147I AGGCCT 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 11
EcoRII CCWGG 2 cut(s) 289, 345
EcoT14I CCWWGG 2 cut(s) 184, 698
EcoT38I GRGCYC 1 cut(s) 241
ErhI CCWWGG 2 cut(s) 184, 698
FaeI CATG 8 cut(s) 82, 218, 245, 328, 333, 364, 557, 702
FatI CATG 8 cut(s) 78, 214, 241, 324, 329, 360, 553, 698
FblI GTMKAC 1 cut(s) 517
Fnu4HI GCNGC 5 cut(s) 63, 99, 688, 750, 753
FokI GGATG 3 cut(s) 32, 585, 785
FriOI GRGCYC 1 cut(s) 241
Fsp4HI GCNGC 5 cut(s) 63, 99, 688, 750, 753
FspBI CTAG 3 cut(s) 717, 756, 842
GlaI GCGC 1 cut(s) 121
GluI GCNGC 5 cut(s) 63, 99, 688, 750, 753
HaeIII GGCC 1 cut(s) 183
HapII CCGG 1 cut(s) 155
HgaI GACGC 1 cut(s) 356
HhaI GCGC 1 cut(s) 122
Hin1II CATG 8 cut(s) 82, 218, 245, 328, 333, 364, 557, 702
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HinfI GANTC 3 cut(s) 392, 801, 838
HpaII CCGG 1 cut(s) 155
HphI GGTGA 2 cut(s) 103, 401
Hpy166II GTNNAC 1 cut(s) 518
Hpy188I TCNGA 1 cut(s) 135
Hpy188III TCNNGA 4 cut(s) 9, 412, 717, 770
Hpy8I GTNNAC 1 cut(s) 518
Hpy99I CGWCG 1 cut(s) 661
HpyAV CCTTC 6 cut(s) 88, 300, 563, 616, 727, 731
HpyCH4III ACNGT 2 cut(s) 515, 547
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 7 cut(s) 78, 377, 420, 491, 510, 797, 824
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 330
HpyF3I CTNAG 1 cut(s) 596
HpySE526I ACGT 1 cut(s) 108
Hsp92II CATG 8 cut(s) 82, 218, 245, 328, 333, 364, 557, 702
HspAI GCGC 1 cut(s) 120
Kzo9I GATC 2 cut(s) 337, 775
LmnI GCTCC 3 cut(s) 117, 236, 364
Lsp1109I GCAGC 5 cut(s) 74, 110, 674, 736, 739
LweI GCATC 4 cut(s) 198, 563, 621, 736
MaeI CTAG 3 cut(s) 717, 756, 842
MaeII ACGT 1 cut(s) 108
MaeIII GTNAC 1 cut(s) 389
MalI GATC 2 cut(s) 339, 777
MboI GATC 2 cut(s) 337, 775
MboII GAAGA 1 cut(s) 446
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 1 cut(s) 241
MluCI AATT 3 cut(s) 312, 562, 808
MlyI GAGTC 2 cut(s) 386, 832
MnlI CCTC 6 cut(s) 47, 51, 76, 262, 288, 633
MroXI GAANNNNTTC 1 cut(s) 499
MseI TTAA 3 cut(s) 111, 708, 812
MslI CAYNNNNRTG 2 cut(s) 323, 429
MspI CCGG 1 cut(s) 155
MspR9I CCNGG 2 cut(s) 291, 347
MvaI CCWGG 2 cut(s) 291, 347
MwoI GCNNNNNNNGC 2 cut(s) 236, 330
NcoI CCATGG 1 cut(s) 698
NdeII GATC 2 cut(s) 337, 775
NlaIII CATG 8 cut(s) 82, 218, 245, 328, 333, 364, 557, 702
NlaIV GGNNCC 2 cut(s) 238, 339
NmuCI GTSAC 1 cut(s) 389
NspI RCATGY 2 cut(s) 82, 333
PceI AGGCCT 1 cut(s) 183
PdmI GAANNNNTTC 1 cut(s) 499
PfeI GAWTC 1 cut(s) 801
PflMI CCANNNNNTGG 1 cut(s) 20
PkrI GCNGC 5 cut(s) 64, 100, 689, 751, 754
PleI GAGTC 2 cut(s) 386, 832
PpsI GAGTC 2 cut(s) 386, 832
PsiI TTATAA 1 cut(s) 447
Psp6I CCWGG 2 cut(s) 289, 345
PspGI CCWGG 2 cut(s) 289, 345
PspN4I GGNNCC 2 cut(s) 238, 339
PspPI GGNCC 1 cut(s) 11
PsuI RGATCY 1 cut(s) 337
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
RseI CAYNNNNRTG 2 cut(s) 323, 429
SaqAI TTAA 3 cut(s) 111, 708, 812
SatI GCNGC 5 cut(s) 63, 99, 688, 750, 753
Sau3AI GATC 2 cut(s) 337, 775
Sau96I GGNCC 1 cut(s) 11
ScaI AGTACT 1 cut(s) 248
SchI GAGTC 2 cut(s) 386, 832
ScrFI CCNGG 2 cut(s) 291, 347
SduI GDGCHC 1 cut(s) 241
SfaNI GCATC 4 cut(s) 198, 563, 621, 736
SinI GGWCC 1 cut(s) 11
SmiMI CAYNNNNRTG 2 cut(s) 323, 429
Sse9I AATT 3 cut(s) 312, 562, 808
SseBI AGGCCT 1 cut(s) 183
SsiI CCGC 2 cut(s) 258, 835
SspMI CTAG 3 cut(s) 717, 756, 842
StuI AGGCCT 1 cut(s) 183
StyD4I CCNGG 2 cut(s) 289, 345
StyI CCWWGG 2 cut(s) 184, 698
TaaI ACNGT 2 cut(s) 515, 547
TaiI ACGT 1 cut(s) 111
TaqI TCGA 4 cut(s) 267, 280, 484, 629
TasI AATT 3 cut(s) 312, 562, 808
TatI WGTACW 1 cut(s) 246
TfiI GAWTC 1 cut(s) 801
Tru1I TTAA 3 cut(s) 111, 708, 812
Tru9I TTAA 3 cut(s) 111, 708, 812
TscAI CASTG 1 cut(s) 550
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 5 cut(s) 62, 98, 687, 749, 752
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 4 cut(s) 17, 203, 307, 349
TspRI CASTG 1 cut(s) 550
Van91I CCANNNNNTGG 1 cut(s) 20
VpaK11BI GGWCC 1 cut(s) 11
XapI RAATTY 1 cut(s) 808
XbaI TCTAGA 1 cut(s) 716
XceI RCATGY 2 cut(s) 82, 333
XmiI GTMKAC 1 cut(s) 517
XmnI GAANNNNTTC 1 cut(s) 499
XspI CTAG 3 cut(s) 717, 756, 842
ZrmI AGTACT 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.