RchiOBHm_Chr6g0263091

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0007487
Physical Location & Seq
Reverse (-)
17881160 .. 17883972
2813 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23595

Sequence Viewer

Length: 756 bp
ATGAAGGAAACGAAAGCAAAAAGTATCCCAATGCAACTAACTAGTGTTTTGTGGGCATGCAAGAACGTTTTACTGCAACGTGTAGGGTATCAGACAGCAAATCAGGAGGACTGCATGAATGATGCAAAGTTGCTGTCCTTCGATGATGCCAGCCTCGAAACCAGTACCAATAAGCTCATTACCAAACTTAAGGAGGATGGAGGTCTTTCCCTGGATGTTTTGGAGAATGTGCTGACAAGTATGTCTGATTTAGGGCAAAAAACTGCTGACGCGATCTTGGATTTCAAGAGTAAGATGATTGATATGAAGGACGATAGCTATAAGCTTGTGGTGATGGAATACTTGAGCAGCAGCCTGGAGCTGCTGGACTTCTTTACTGAATTGGAAAAGTTTCTAGACTCGGCTCGTCGGAGCCATTCACATATCGAACATGCGATTGAACAGTGTAGCAGCTTGGGGAGTAAACATAGACCAGGAAGCGAGGAGGCCAAGATGATTTCCAATATATTGAGTAACATTATGGCGTCTAATGTCAATGACCACTCTGGTGAAAAGTTGGTCGAAAAAGCTCATTGTTTGCTTGAGAAGCAGAAAGAAATGTTTTCCAAATGTCGCATTGAACAAGAGAAGCTTGGGAAGGATAAGAAGAGTAATGTTTGGAGGAAGCTCTTGAGTCGTATATTCATGGTTGTCAAGTGGCTCGTTCTCGTTGTTCTAGGGGTGATATTTGTAAACTTAAACAGAAAAAACTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

251

Amino Acids

28.54

Weight (kDa)

7.63

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 46 - 244 3.2e-16 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 241
AccII CGCG 1 cut(s) 272
AclI AACGTT 1 cut(s) 66
AcyI GRCGYC 1 cut(s) 524
AfaI GTAC 1 cut(s) 166
AflII CTTAAG 1 cut(s) 188
AflIII ACRYGT 1 cut(s) 79
AgsI TTSAA 3 cut(s) 286, 440, 620
AhlI ACTAGT 1 cut(s) 41
AjnI CCWGG 3 cut(s) 210, 354, 472
AleI CACNNNNGTG 1 cut(s) 546
AluBI AGCT 8 cut(s) 175, 318, 325, 361, 453, 569, 631, 667
AluI AGCT 8 cut(s) 175, 318, 325, 361, 453, 569, 631, 667
AoxI GGCC 1 cut(s) 486
ApeKI GCWGC 4 cut(s) 348, 351, 361, 450
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
Asp700I GAANNNNTTC 1 cut(s) 390
AsuHPI GGTGA 3 cut(s) 343, 560, 733
BbvI GCAGC 4 cut(s) 348, 360, 363, 462
BccI CCATC 2 cut(s) 191, 328
BciT130I CCWGG 3 cut(s) 212, 356, 474
BciVI GTATCC 1 cut(s) 35
BcuI ACTAGT 1 cut(s) 41
BfaI CTAG 3 cut(s) 42, 395, 716
BfrI CTTAAG 1 cut(s) 188
BfuI GTATCC 1 cut(s) 35
BisI GCNGC 4 cut(s) 349, 352, 362, 451
BlsI GCNGC 4 cut(s) 350, 353, 363, 452
Bme1390I CCNGG 3 cut(s) 212, 356, 474
BmiI GGNNCC 1 cut(s) 413
BmrFI CCNGG 3 cut(s) 212, 356, 474
BmsI GCATC 2 cut(s) 112, 136
BpmI CTGGAG 1 cut(s) 377
BpuEI CTTGAG 3 cut(s) 364, 602, 691
BsaHI GRCGYC 1 cut(s) 524
BsaJI CCNNGG 1 cut(s) 210
Bse1I ACTGG 1 cut(s) 162
BseBI CCWGG 3 cut(s) 212, 356, 474
BseDI CCNNGG 1 cut(s) 210
BseGI GGATG 2 cut(s) 202, 220
BseNI ACTGG 1 cut(s) 162
BseRI GAGGAG 1 cut(s) 497
BseXI GCAGC 4 cut(s) 348, 360, 363, 462
Bsh1236I CGCG 1 cut(s) 272
BshFI GGCC 1 cut(s) 488
BsnI GGCC 1 cut(s) 488
Bsp143I GATC 1 cut(s) 273
BspANI GGCC 1 cut(s) 488
BspFNI CGCG 1 cut(s) 272
BspLI GGNNCC 1 cut(s) 413
BspTI CTTAAG 1 cut(s) 188
BsrI ACTGG 1 cut(s) 162
BssECI CCNNGG 1 cut(s) 210
BssMI GATC 1 cut(s) 273
BssNI GRCGYC 1 cut(s) 524
Bst2UI CCWGG 3 cut(s) 212, 356, 474
Bst4CI ACNGT 1 cut(s) 444
Bst6I CTCTTC 1 cut(s) 641
BstACI GRCGYC 1 cut(s) 524
BstAFI CTTAAG 1 cut(s) 188
BstC8I GCNNGC 2 cut(s) 58, 151
BstF5I GGATG 2 cut(s) 202, 220
BstFNI CGCG 1 cut(s) 272
BstKTI GATC 1 cut(s) 276
BstMBI GATC 1 cut(s) 273
BstMWI GCNNNNNNNGC 1 cut(s) 586
BstNI CCWGG 3 cut(s) 212, 356, 474
BstNSI RCATGY 2 cut(s) 60, 434
BstSCI CCNGG 3 cut(s) 210, 354, 472
BstUI CGCG 1 cut(s) 272
BstV1I GCAGC 4 cut(s) 348, 360, 363, 462
BsuI GTATCC 1 cut(s) 35
BsuRI GGCC 1 cut(s) 488
BtsCI GGATG 2 cut(s) 202, 220
BtsIMutI CAGTG 1 cut(s) 449
Cac8I GCNNGC 2 cut(s) 58, 151
CseI GACGC 2 cut(s) 278, 513
Csp6I GTAC 1 cut(s) 165
CviAII CATG 4 cut(s) 57, 115, 431, 685
CviQI GTAC 1 cut(s) 165
DpnI GATC 1 cut(s) 275
DpnII GATC 1 cut(s) 273
DrdI GACNNNNNNGTC 1 cut(s) 241
DseDI GACNNNNNNGTC 1 cut(s) 241
Eam1104I CTCTTC 1 cut(s) 641
EarI CTCTTC 1 cut(s) 641
EcoRII CCWGG 3 cut(s) 210, 354, 472
FaeI CATG 4 cut(s) 60, 118, 434, 688
FalI AAGNNNNNCTT 2 cut(s) 615, 647
FatI CATG 4 cut(s) 56, 114, 430, 684
Fnu4HI GCNGC 4 cut(s) 349, 352, 362, 451
FokI GGATG 2 cut(s) 209, 227
Fsp4HI GCNGC 4 cut(s) 349, 352, 362, 451
FspBI CTAG 3 cut(s) 42, 395, 716
GluI GCNGC 4 cut(s) 349, 352, 362, 451
GsuI CTGGAG 1 cut(s) 377
HaeIII GGCC 1 cut(s) 488
HgaI GACGC 2 cut(s) 278, 513
Hin1I GRCGYC 1 cut(s) 524
Hin1II CATG 4 cut(s) 60, 118, 434, 688
HindIII AAGCTT 2 cut(s) 323, 629
HinfI GANTC 2 cut(s) 398, 673
HphI GGTGA 3 cut(s) 343, 560, 733
Hpy166II GTNNAC 2 cut(s) 464, 733
Hpy188I TCNGA 3 cut(s) 93, 247, 411
Hpy188III TCNNGA 4 cut(s) 104, 286, 395, 670
Hpy8I GTNNAC 2 cut(s) 464, 733
Hpy99I CGWCG 1 cut(s) 411
HpyAV CCTTC 3 cut(s) 148, 301, 631
HpyCH4III ACNGT 1 cut(s) 444
HpyCH4IV ACGT 2 cut(s) 66, 79
HpyCH4V TGCA 5 cut(s) 34, 60, 76, 114, 125
HpyF10VI GCNNNNNNNGC 1 cut(s) 586
HpySE526I ACGT 2 cut(s) 66, 79
Hsp92I GRCGYC 1 cut(s) 524
Hsp92II CATG 4 cut(s) 60, 118, 434, 688
Kzo9I GATC 1 cut(s) 273
LmnI GCTCC 2 cut(s) 358, 411
Lsp1109I GCAGC 4 cut(s) 348, 360, 363, 462
LweI GCATC 2 cut(s) 112, 136
MaeI CTAG 3 cut(s) 42, 395, 716
MaeII ACGT 2 cut(s) 66, 79
MaeIII GTNAC 1 cut(s) 512
MalI GATC 1 cut(s) 275
MboI GATC 1 cut(s) 273
MboII GAAGA 1 cut(s) 658
MluCI AATT 1 cut(s) 380
MlyI GAGTC 2 cut(s) 392, 682
MmeI TCCRAC 1 cut(s) 389
MnlI CCTC 7 cut(s) 100, 164, 187, 194, 475, 478, 654
MroXI GAANNNNTTC 1 cut(s) 390
MseI TTAA 3 cut(s) 189, 737, 754
MslI CAYNNNNRTG 1 cut(s) 546
MspCI CTTAAG 1 cut(s) 188
MspR9I CCNGG 3 cut(s) 212, 356, 474
MvaI CCWGG 3 cut(s) 212, 356, 474
MvnI CGCG 1 cut(s) 272
MwoI GCNNNNNNNGC 1 cut(s) 586
NdeII GATC 1 cut(s) 273
NlaIII CATG 4 cut(s) 60, 118, 434, 688
NlaIV GGNNCC 1 cut(s) 413
NmeAIII GCCGAG 1 cut(s) 380
NspI RCATGY 2 cut(s) 60, 434
OliI CACNNNNGTG 1 cut(s) 546
PaeI GCATGC 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 390
PkrI GCNGC 4 cut(s) 350, 353, 363, 452
PleI GAGTC 2 cut(s) 392, 681
PpsI GAGTC 2 cut(s) 392, 681
Psp1406I AACGTT 1 cut(s) 66
Psp6I CCWGG 3 cut(s) 210, 354, 472
PspGI CCWGG 3 cut(s) 210, 354, 472
PspN4I GGNNCC 1 cut(s) 413
RsaI GTAC 1 cut(s) 166
RsaNI GTAC 1 cut(s) 165
RseI CAYNNNNRTG 1 cut(s) 546
SaqAI TTAA 3 cut(s) 189, 737, 754
SatI GCNGC 4 cut(s) 349, 352, 362, 451
Sau3AI GATC 1 cut(s) 273
SchI GAGTC 2 cut(s) 392, 682
ScrFI CCNGG 3 cut(s) 212, 356, 474
SfaNI GCATC 2 cut(s) 112, 136
SmiMI CAYNNNNRTG 1 cut(s) 546
SmlI CTYRAG 4 cut(s) 188, 343, 581, 670
SmoI CTYRAG 4 cut(s) 188, 343, 581, 670
SpeI ACTAGT 1 cut(s) 41
SphI GCATGC 1 cut(s) 60
Sse9I AATT 1 cut(s) 380
SspMI CTAG 3 cut(s) 42, 395, 716
StyD4I CCNGG 3 cut(s) 210, 354, 472
TaaI ACNGT 1 cut(s) 444
TaiI ACGT 2 cut(s) 69, 82
TaqI TCGA 4 cut(s) 141, 156, 426, 561
TasI AATT 1 cut(s) 380
Tru1I TTAA 3 cut(s) 189, 737, 754
Tru9I TTAA 3 cut(s) 189, 737, 754
TscAI CASTG 1 cut(s) 449
TseI GCWGC 4 cut(s) 348, 351, 361, 450
TspDTI ATGAA 4 cut(s) 17, 131, 320, 673
TspRI CASTG 1 cut(s) 449
Vha464I CTTAAG 1 cut(s) 188
XbaI TCTAGA 1 cut(s) 394
XceI RCATGY 2 cut(s) 60, 434
XmnI GAANNNNTTC 1 cut(s) 390
XspI CTAG 3 cut(s) 42, 395, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.