RLG00000014373

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0007487
Physical Location & Seq
Forward (+)
52287042 .. 52288272
1231 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014373

Sequence Viewer

Length: 1167 bp
ATGAAGGAAACGAAATCGAAAATTATTCCAATGCAACTAACTAATGCTTTGTGGGCATGCAAGAACGTTTTAATGCAACATCTAGGGTTGACGGCAGCAAATCAGGAGGACTGCAAGAGTGATGCAGAGTTCCGGTCCTTCAATGATGCCAGCCTCAAAACCGGTACCAATGAGATCATTACCAAATTTAAGGAGGATGGAGGTCTTTCCTTGGATGTTTTGGACAATGTGCTGACAAGTATGTCTGATTTGGGGCAAAAAACAGCTGACGCGATCTTGGATTTCAAAAGTAGTAAGATGATTCCCATGAAGGACGATAGCTATAAGCTTGTGGTGAAGGAGTACTTGAGCAGCAGCTTGGAGCTGCTGGAGTTCTTTACTGAACTGGAAAAGTTTCTAGACTCAGCTAGTCGGAGCCATTCACATATCGAACATGCGATTGAAGAGTGTAGCAGCTTGGGGAGTAAACATGGAGTAGGAAGGGAGGAGGCCAAGATGATTTTGAATATATTGAGTAACATTATGAAGTCTAATGTCAATGACTACTCTGGTGAAAAGTTGGTCGAAAAGGCTCGTTGTTTGCTTGAGAAGCAGGAAGAAATGATTGACAAATGTCGCATTGAACAAGAGAAGCTTGGGAAGGATCAGAAGACTATTAATGGTTGGAAGAAGCTCTTGAATTATATATTCATGGGTGCTAAGTGCCTCATTATCGTTGTTCTAGTTGTGACATTGGCCAATTCTATCAAATCTGTTAGAGATGGTTCGTCTGATATAGTTGGGGATTTGAAAATCAAAGCAGCATGTTACTTTGAGGTCTCTAGTATAGTAGAAATAGCAGATCACTGGAGTAGCTCCCGCCTTGAAGTATGTCAAAATGATTTTATGCTGAAGAATGATGCAATAAACACAATACAGGAAGGAGCTTTTCATTCCATAAAAGAAATGGGGGAAATTGAAGTTCTCATCTTTAAGGTAGTAAATTCCATTGACTCTCTCCTTGCTGTAAGTACAAACTTTGCAAATGGTGGAAATGTAGAAGTGGCAATCAAGAATATTAAGCTTGACCTGAAGAAGTTTTTGGATAAAATTAACGCTTTGGAGGAGAAAACAGAGGAATGCAGCTCTAGAATTTTGAAAGCAAGGCAAGCTGTTCTCGCGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

389

Amino Acids

43.37

Weight (kDa)

5.58

Isoelectric Point (pI)

37.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 47 - 386 8.2e-32 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 241
Acc65I GGTACC 1 cut(s) 164
AccB1I GGYRCC 1 cut(s) 164
AccII CGCG 2 cut(s) 272, 1160
AciI CCGC 1 cut(s) 859
AclI AACGTT 1 cut(s) 66
AclWI GGATC 1 cut(s) 651
AcoI YGGCCR 1 cut(s) 735
AcsI RAATTY 3 cut(s) 185, 982, 1131
AcuI CTGAAG 2 cut(s) 911, 1091
AfaI GTAC 3 cut(s) 166, 344, 1012
AgeI ACCGGT 1 cut(s) 161
AjuI GAANNNNNNNTTGG 2 cut(s) 1064, 1096
Alw26I GTCTC 1 cut(s) 823
AlwI GGATC 1 cut(s) 651
AoxI GGCC 2 cut(s) 489, 735
ApeKI GCWGC 7 cut(s) 95, 351, 354, 364, 453, 800, 1122
ApoI RAATTY 3 cut(s) 185, 982, 1131
AseI ATTAAT 1 cut(s) 657
AsiGI ACCGGT 1 cut(s) 161
Asp700I GAANNNNTTC 1 cut(s) 393
Asp718I GGTACC 1 cut(s) 164
AspS9I GGNCC 1 cut(s) 135
AsuHPI GGTGA 2 cut(s) 346, 563
AvaII GGWCC 1 cut(s) 135
BalI TGGCCA 1 cut(s) 737
BanI GGYRCC 1 cut(s) 164
BbsI GAAGAC 1 cut(s) 656
BbvI GCAGC 7 cut(s) 107, 351, 363, 366, 465, 812, 1134
BccI CCATC 2 cut(s) 191, 755
BceAI ACGGC 1 cut(s) 108
BcoDI GTCTC 1 cut(s) 823
BfaI CTAG 6 cut(s) 83, 398, 408, 722, 822, 1128
BisI GCNGC 7 cut(s) 96, 352, 355, 365, 454, 801, 1123
BlsI GCNGC 7 cut(s) 97, 353, 356, 366, 455, 802, 1124
BmcAI AGTACT 1 cut(s) 344
Bme18I GGWCC 1 cut(s) 135
BmgT120I GGNCC 1 cut(s) 135
BmiI GGNNCC 2 cut(s) 166, 416
BmsI GCATC 3 cut(s) 112, 136, 889
BoxI GACNNNNGTC 1 cut(s) 612
BpiI GAAGAC 1 cut(s) 656
BpmI CTGGAG 2 cut(s) 389, 868
BpuEI CTTGAG 2 cut(s) 367, 605
BsaI GGTCTC 1 cut(s) 823
BsaJI CCNNGG 1 cut(s) 210
BsaWI WCCGGW 2 cut(s) 132, 161
Bse118I RCCGGY 1 cut(s) 161
Bse1I ACTGG 2 cut(s) 390, 851
BseDI CCNNGG 1 cut(s) 210
BseGI GGATG 2 cut(s) 202, 220
BseMII CTCAG 1 cut(s) 417
BseNI ACTGG 2 cut(s) 390, 851
BseRI GAGGAG 2 cut(s) 500, 1118
BseXI GCAGC 7 cut(s) 107, 351, 363, 366, 465, 812, 1134
Bsh1236I CGCG 2 cut(s) 272, 1160
BshFI GGCC 2 cut(s) 491, 737
BshNI GGYRCC 1 cut(s) 164
BshTI ACCGGT 1 cut(s) 161
BsiSI CCGG 2 cut(s) 133, 162
BsmAI GTCTC 1 cut(s) 823
BsmI GAATGC 1 cut(s) 1124
BsnI GGCC 2 cut(s) 491, 737
Bso31I GGTCTC 1 cut(s) 823
Bsp143I GATC 4 cut(s) 174, 273, 643, 841
BspACI CCGC 1 cut(s) 859
BspANI GGCC 2 cut(s) 491, 737
BspCNI CTCAG 1 cut(s) 416
BspFNI CGCG 2 cut(s) 272, 1160
BspLI GGNNCC 2 cut(s) 166, 416
BspPI GGATC 1 cut(s) 651
BspT107I GGYRCC 1 cut(s) 164
BspTNI GGTCTC 1 cut(s) 823
BsrFI RCCGGY 1 cut(s) 161
BsrI ACTGG 2 cut(s) 390, 851
BssAI RCCGGY 1 cut(s) 161
BssECI CCNNGG 1 cut(s) 210
BssMI GATC 4 cut(s) 174, 273, 643, 841
BssT1I CCWWGG 1 cut(s) 210
Bst6I CTCTTC 1 cut(s) 438
BstC8I GCNNGC 3 cut(s) 58, 151, 1149
BstDEI CTNAG 2 cut(s) 403, 699
BstF5I GGATG 2 cut(s) 202, 220
BstFNI CGCG 2 cut(s) 272, 1160
BstKTI GATC 4 cut(s) 177, 276, 646, 844
BstMAI GTCTC 1 cut(s) 823
BstMBI GATC 4 cut(s) 174, 273, 643, 841
BstMWI GCNNNNNNNGC 4 cut(s) 53, 589, 1148, 1157
BstNSI RCATGY 3 cut(s) 60, 437, 807
BstPAI GACNNNNGTC 1 cut(s) 612
BstUI CGCG 2 cut(s) 272, 1160
BstV1I GCAGC 7 cut(s) 107, 351, 363, 366, 465, 812, 1134
BstV2I GAAGAC 1 cut(s) 656
BsuRI GGCC 2 cut(s) 491, 737
BtsCI GGATG 2 cut(s) 202, 220
BtsIMutI CAGTG 1 cut(s) 844
Cac8I GCNNGC 3 cut(s) 58, 151, 1149
Cfr10I RCCGGY 1 cut(s) 161
Cfr13I GGNCC 1 cut(s) 135
CseI GACGC 1 cut(s) 278
Csp6I GTAC 3 cut(s) 165, 343, 1011
CspAI ACCGGT 1 cut(s) 161
CviAII CATG 6 cut(s) 57, 307, 434, 470, 691, 804
CviQI GTAC 3 cut(s) 165, 343, 1011
DdeI CTNAG 2 cut(s) 403, 699
DpnI GATC 4 cut(s) 176, 275, 645, 843
DpnII GATC 4 cut(s) 174, 273, 643, 841
DrdI GACNNNNNNGTC 1 cut(s) 241
DseDI GACNNNNNNGTC 1 cut(s) 241
EaeI YGGCCR 1 cut(s) 735
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Eco130I CCWWGG 1 cut(s) 210
Eco31I GGTCTC 1 cut(s) 823
Eco47I GGWCC 1 cut(s) 135
Eco57I CTGAAG 2 cut(s) 911, 1091
EcoT14I CCWWGG 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 210
FaeI CATG 6 cut(s) 60, 310, 437, 473, 694, 807
FalI AAGNNNNNCTT 6 cut(s) 329, 361, 618, 650, 659, 691
FatI CATG 6 cut(s) 56, 306, 433, 469, 690, 803
FauI CCCGC 1 cut(s) 866
Fnu4HI GCNGC 7 cut(s) 96, 352, 355, 365, 454, 801, 1123
FokI GGATG 2 cut(s) 209, 227
Fsp4HI GCNGC 7 cut(s) 96, 352, 355, 365, 454, 801, 1123
FspBI CTAG 6 cut(s) 83, 398, 408, 722, 822, 1128
GluI GCNGC 7 cut(s) 96, 352, 355, 365, 454, 801, 1123
GsuI CTGGAG 2 cut(s) 389, 868
HaeIII GGCC 2 cut(s) 491, 737
HapII CCGG 2 cut(s) 133, 162
HgaI GACGC 1 cut(s) 278
Hin1II CATG 6 cut(s) 60, 310, 437, 473, 694, 807
HincII GTYRAC 1 cut(s) 90
HindII GTYRAC 1 cut(s) 90
HindIII AAGCTT 3 cut(s) 326, 632, 1061
HinfI GANTC 3 cut(s) 301, 401, 992
HpaII CCGG 2 cut(s) 133, 162
HphI GGTGA 2 cut(s) 346, 563
Hpy166II GTNNAC 2 cut(s) 90, 467
Hpy188I TCNGA 4 cut(s) 247, 414, 648, 772
Hpy188III TCNNGA 5 cut(s) 104, 398, 676, 1051, 1128
Hpy8I GTNNAC 2 cut(s) 90, 467
HpyAV CCTTC 6 cut(s) 148, 304, 331, 474, 634, 914
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 8 cut(s) 34, 60, 76, 114, 125, 902, 1022, 1122
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 589, 1148, 1157
HpyF3I CTNAG 2 cut(s) 403, 699
HpySE526I ACGT 1 cut(s) 66
Hsp92II CATG 6 cut(s) 60, 310, 437, 473, 694, 807
KpnI GGTACC 1 cut(s) 168
Kzo9I GATC 4 cut(s) 174, 273, 643, 841
LmnI GCTCC 4 cut(s) 361, 414, 860, 923
Lsp1109I GCAGC 7 cut(s) 107, 351, 363, 366, 465, 812, 1134
LweI GCATC 3 cut(s) 112, 136, 889
MaeI CTAG 6 cut(s) 83, 398, 408, 722, 822, 1128
MaeII ACGT 1 cut(s) 66
MaeIII GTNAC 3 cut(s) 515, 727, 806
MalI GATC 4 cut(s) 176, 275, 645, 843
MboI GATC 4 cut(s) 174, 273, 643, 841
MboII GAAGA 6 cut(s) 455, 608, 661, 679, 904, 1084
MlsI TGGCCA 1 cut(s) 737
MluCI AATT 8 cut(s) 21, 185, 679, 739, 954, 982, 1089, 1131
MluNI TGGCCA 1 cut(s) 737
MlyI GAGTC 2 cut(s) 395, 986
MmeI TCCRAC 2 cut(s) 392, 644
Mox20I TGGCCA 1 cut(s) 737
MroXI GAANNNNTTC 1 cut(s) 393
MscI TGGCCA 1 cut(s) 737
MseI TTAA 6 cut(s) 71, 189, 657, 972, 1059, 1092
Msp20I TGGCCA 1 cut(s) 737
MspA1I CMGCKG 1 cut(s) 266
MspI CCGG 2 cut(s) 133, 162
Mva1269I GAATGC 1 cut(s) 1124
MvnI CGCG 2 cut(s) 272, 1160
MwoI GCNNNNNNNGC 4 cut(s) 53, 589, 1148, 1157
NdeII GATC 4 cut(s) 174, 273, 643, 841
NlaIII CATG 6 cut(s) 60, 310, 437, 473, 694, 807
NlaIV GGNNCC 2 cut(s) 166, 416
NmuCI GTSAC 1 cut(s) 727
NspI RCATGY 3 cut(s) 60, 437, 807
PaeI GCATGC 1 cut(s) 60
PctI GAATGC 1 cut(s) 1124
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 1 cut(s) 301
PinAI ACCGGT 1 cut(s) 161
PkrI GCNGC 7 cut(s) 97, 353, 356, 366, 455, 802, 1124
PleI GAGTC 2 cut(s) 395, 986
PpsI GAGTC 2 cut(s) 395, 986
PshAI GACNNNNGTC 1 cut(s) 612
PshBI ATTAAT 1 cut(s) 657
Psp1406I AACGTT 1 cut(s) 66
PspN4I GGNNCC 2 cut(s) 166, 416
PspPI GGNCC 1 cut(s) 135
PvuII CAGCTG 1 cut(s) 266
RsaI GTAC 3 cut(s) 166, 344, 1012
RsaNI GTAC 3 cut(s) 165, 343, 1011
SaqAI TTAA 6 cut(s) 71, 189, 657, 972, 1059, 1092
SatI GCNGC 7 cut(s) 96, 352, 355, 365, 454, 801, 1123
Sau3AI GATC 4 cut(s) 174, 273, 643, 841
Sau96I GGNCC 1 cut(s) 135
ScaI AGTACT 1 cut(s) 344
SchI GAGTC 2 cut(s) 395, 986
SfaNI GCATC 3 cut(s) 112, 136, 889
SinI GGWCC 1 cut(s) 135
SmlI CTYRAG 2 cut(s) 346, 584
SmoI CTYRAG 2 cut(s) 346, 584
SphI GCATGC 1 cut(s) 60
Sse9I AATT 8 cut(s) 21, 185, 679, 739, 954, 982, 1089, 1131
SsiI CCGC 1 cut(s) 859
SspI AATATT 1 cut(s) 1057
SspMI CTAG 6 cut(s) 83, 398, 408, 722, 822, 1128
StyI CCWWGG 1 cut(s) 210
TaiI ACGT 1 cut(s) 69
TaqI TCGA 3 cut(s) 17, 429, 564
TasI AATT 8 cut(s) 21, 185, 679, 739, 954, 982, 1089, 1131
TatI WGTACW 2 cut(s) 342, 1010
TfiI GAWTC 1 cut(s) 301
Tru1I TTAA 6 cut(s) 71, 189, 657, 972, 1059, 1092
Tru9I TTAA 6 cut(s) 71, 189, 657, 972, 1059, 1092
TscAI CASTG 1 cut(s) 851
TseFI GTSAC 1 cut(s) 727
TseI GCWGC 7 cut(s) 95, 351, 354, 364, 453, 800, 1122
Tsp45I GTSAC 1 cut(s) 727
TspDTI ATGAA 5 cut(s) 17, 323, 539, 679, 920
TspRI CASTG 1 cut(s) 851
VpaK11BI GGWCC 1 cut(s) 135
VspI ATTAAT 1 cut(s) 657
XapI RAATTY 3 cut(s) 185, 982, 1131
XbaI TCTAGA 2 cut(s) 397, 1127
XceI RCATGY 3 cut(s) 60, 437, 807
XcmI CCANNNNNNNNNTGG 1 cut(s) 943
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 6 cut(s) 83, 398, 408, 722, 822, 1128
ZrmI AGTACT 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.