Rroxscaffold_7G00204710

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0007487
Physical Location & Seq
Forward (+)
54182797 .. 54183719
923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00204710.1

Sequence Viewer

Length: 828 bp
ATGCAACTAACTAGTGCTTTGTGGGCATGCAAGAACGTTTTACTGCAACGTGTAGGGAATCAGACAGCAAATCAGGAGGACTGCATGAATGATGCAAAGTTGCTGTCCTTCGATGATGCCAGCCTCGAAACCAGTACCAATAAGCTCATTACCAAACTTAAGGAGGATGGAGGTCTTTCCCTGGATGTTTTGGAGAATGTGCTGACAAGTATGTCTGATTTAGGGCAAAAAACTGCTGACGCGATCTTGGATTTCAAGAGTAAGATGATTGATATGAAGGACGATAGCTATAAGCTTGTGGTGATGGAATACTTGAGCAGCAGCCTGGAGCTGCTGGACTTCTTTACTGAATTGGAAAAGTTTCTAGACTCGGCTCGTCGGAGCCATTCACATATCGAACATGCGATTGAACAGTGTAGCAGCTTGGGATTGGTCGAAAAAGCTCATTGTTTGCTTGAGAAGCAGAAAGAAATGTTTTCCAAATATCGCATTGAACAAGAGAAGCTTGGGAAGGATAAGAAGAGTAATGTTTGGAGGAAGCTCTTGAGTCGTATATTCATGGTTGTCAAGTGGCTCGTTCTCGTTGTTCTAGGGGTGATATTGGCCTATACGCCCTATAATATAACATCTGATGGAGATGGTTTGTCTAATGTGGTTGGAGATTTGAAAATCAAAGCAGCATGTTTCCTTGATGTCTCTGTTATAACAGAAATAGTAGATTACTGGAATAGCTCCCACCTTGAAGTATGTCAAGATGATTTTGGGCTGAAGAATGAAGCAATGAACATAATACAGGAAGGAGCTTTGAAAGAAAGAAGATTTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

275

Amino Acids

31.09

Weight (kDa)

5.18

Isoelectric Point (pI)

40.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 36 - 138 4.8e-11 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 704
AasI GACNNNNNNGTC 1 cut(s) 211
AccII CGCG 1 cut(s) 242
AclI AACGTT 1 cut(s) 36
AcuI CTGAAG 1 cut(s) 788
AfaI GTAC 1 cut(s) 136
AflII CTTAAG 1 cut(s) 158
AflIII ACRYGT 1 cut(s) 49
AgsI TTSAA 6 cut(s) 256, 410, 494, 667, 743, 808
AhlI ACTAGT 1 cut(s) 11
AjnI CCWGG 2 cut(s) 180, 324
AjuI GAANNNNNNNTTGG 1 cut(s) 804
Alw26I GTCTC 1 cut(s) 700
AoxI GGCC 1 cut(s) 603
ApeKI GCWGC 5 cut(s) 318, 321, 331, 420, 677
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
Asp700I GAANNNNTTC 1 cut(s) 360
AsuHPI GGTGA 2 cut(s) 313, 607
BbvI GCAGC 5 cut(s) 318, 330, 333, 432, 689
BccI CCATC 4 cut(s) 161, 298, 626, 632
BciT130I CCWGG 2 cut(s) 182, 326
BcoDI GTCTC 1 cut(s) 700
BcuI ACTAGT 1 cut(s) 11
BfaI CTAG 3 cut(s) 12, 365, 590
BfrI CTTAAG 1 cut(s) 158
BisI GCNGC 5 cut(s) 319, 322, 332, 421, 678
BlsI GCNGC 5 cut(s) 320, 323, 333, 422, 679
Bme1390I CCNGG 2 cut(s) 182, 326
BmiI GGNNCC 1 cut(s) 383
BmrFI CCNGG 2 cut(s) 182, 326
BmsI GCATC 2 cut(s) 82, 106
BpmI CTGGAG 1 cut(s) 347
BpuEI CTTGAG 3 cut(s) 334, 476, 565
BsaJI CCNNGG 1 cut(s) 180
Bse1I ACTGG 2 cut(s) 132, 728
Bse3DI GCAATG 1 cut(s) 786
BseBI CCWGG 2 cut(s) 182, 326
BseDI CCNNGG 1 cut(s) 180
BseGI GGATG 2 cut(s) 172, 190
BseMI GCAATG 1 cut(s) 786
BseNI ACTGG 2 cut(s) 132, 728
BseXI GCAGC 5 cut(s) 318, 330, 333, 432, 689
Bsh1236I CGCG 1 cut(s) 242
BshFI GGCC 1 cut(s) 605
BsmAI GTCTC 1 cut(s) 700
BsnI GGCC 1 cut(s) 605
Bsp143I GATC 1 cut(s) 243
BspANI GGCC 1 cut(s) 605
BspFNI CGCG 1 cut(s) 242
BspLI GGNNCC 1 cut(s) 383
BspTI CTTAAG 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 786
BsrI ACTGG 2 cut(s) 132, 728
BssECI CCNNGG 1 cut(s) 180
BssMI GATC 1 cut(s) 243
Bst2UI CCWGG 2 cut(s) 182, 326
Bst4CI ACNGT 1 cut(s) 414
Bst6I CTCTTC 1 cut(s) 515
BstAFI CTTAAG 1 cut(s) 158
BstC8I GCNNGC 2 cut(s) 28, 121
BstF5I GGATG 2 cut(s) 172, 190
BstFNI CGCG 1 cut(s) 242
BstKTI GATC 1 cut(s) 246
BstMAI GTCTC 1 cut(s) 700
BstMBI GATC 1 cut(s) 243
BstMWI GCNNNNNNNGC 2 cut(s) 23, 460
BstNI CCWGG 2 cut(s) 182, 326
BstNSI RCATGY 3 cut(s) 30, 404, 684
BstSCI CCNGG 2 cut(s) 180, 324
BstUI CGCG 1 cut(s) 242
BstV1I GCAGC 5 cut(s) 318, 330, 333, 432, 689
BsuRI GGCC 1 cut(s) 605
BtsCI GGATG 2 cut(s) 172, 190
BtsIMutI CAGTG 1 cut(s) 419
Cac8I GCNNGC 2 cut(s) 28, 121
CseI GACGC 1 cut(s) 248
Csp6I GTAC 1 cut(s) 135
CviAII CATG 5 cut(s) 27, 85, 401, 559, 681
CviQI GTAC 1 cut(s) 135
DpnI GATC 1 cut(s) 245
DpnII GATC 1 cut(s) 243
DrdI GACNNNNNNGTC 1 cut(s) 211
DseDI GACNNNNNNGTC 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 515
EarI CTCTTC 1 cut(s) 515
Eco57I CTGAAG 1 cut(s) 788
EcoRII CCWGG 2 cut(s) 180, 324
FaeI CATG 5 cut(s) 30, 88, 404, 562, 684
FalI AAGNNNNNCTT 2 cut(s) 489, 521
FatI CATG 5 cut(s) 26, 84, 400, 558, 680
Fnu4HI GCNGC 5 cut(s) 319, 322, 332, 421, 678
FokI GGATG 2 cut(s) 179, 197
Fsp4HI GCNGC 5 cut(s) 319, 322, 332, 421, 678
FspBI CTAG 3 cut(s) 12, 365, 590
GluI GCNGC 5 cut(s) 319, 322, 332, 421, 678
GsuI CTGGAG 1 cut(s) 347
HaeIII GGCC 1 cut(s) 605
HgaI GACGC 1 cut(s) 248
Hin1II CATG 5 cut(s) 30, 88, 404, 562, 684
HindIII AAGCTT 2 cut(s) 293, 503
HinfI GANTC 3 cut(s) 58, 368, 547
HphI GGTGA 2 cut(s) 313, 607
Hpy188I TCNGA 4 cut(s) 63, 217, 381, 631
Hpy188III TCNNGA 5 cut(s) 74, 256, 365, 544, 752
Hpy99I CGWCG 1 cut(s) 381
HpyAV CCTTC 4 cut(s) 118, 271, 505, 791
HpyCH4III ACNGT 1 cut(s) 414
HpyCH4IV ACGT 2 cut(s) 36, 49
HpyCH4V TGCA 5 cut(s) 4, 30, 46, 84, 95
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 460
HpySE526I ACGT 2 cut(s) 36, 49
Hsp92II CATG 5 cut(s) 30, 88, 404, 562, 684
Kzo9I GATC 1 cut(s) 243
LmnI GCTCC 4 cut(s) 328, 381, 737, 800
Lsp1109I GCAGC 5 cut(s) 318, 330, 333, 432, 689
LweI GCATC 2 cut(s) 82, 106
MaeI CTAG 3 cut(s) 12, 365, 590
MaeII ACGT 2 cut(s) 36, 49
MalI GATC 1 cut(s) 245
MboI GATC 1 cut(s) 243
MboII GAAGA 3 cut(s) 532, 781, 828
MluCI AATT 1 cut(s) 350
MlyI GAGTC 2 cut(s) 362, 556
MmeI TCCRAC 2 cut(s) 359, 637
MnlI CCTC 5 cut(s) 70, 134, 157, 164, 528
MroXI GAANNNNTTC 1 cut(s) 360
MseI TTAA 1 cut(s) 159
MspCI CTTAAG 1 cut(s) 158
MspR9I CCNGG 2 cut(s) 182, 326
MvaI CCWGG 2 cut(s) 182, 326
MvnI CGCG 1 cut(s) 242
MwoI GCNNNNNNNGC 2 cut(s) 23, 460
NdeII GATC 1 cut(s) 243
NlaIII CATG 5 cut(s) 30, 88, 404, 562, 684
NlaIV GGNNCC 1 cut(s) 383
NmeAIII GCCGAG 1 cut(s) 350
NspI RCATGY 3 cut(s) 30, 404, 684
PaeI GCATGC 1 cut(s) 30
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 1 cut(s) 58
PkrI GCNGC 5 cut(s) 320, 323, 333, 422, 679
PleI GAGTC 2 cut(s) 362, 555
PpsI GAGTC 2 cut(s) 362, 555
PsiI TTATAA 1 cut(s) 704
Psp1406I AACGTT 1 cut(s) 36
Psp6I CCWGG 2 cut(s) 180, 324
PspGI CCWGG 2 cut(s) 180, 324
PspN4I GGNNCC 1 cut(s) 383
RsaI GTAC 1 cut(s) 136
RsaNI GTAC 1 cut(s) 135
SaqAI TTAA 1 cut(s) 159
SatI GCNGC 5 cut(s) 319, 322, 332, 421, 678
Sau3AI GATC 1 cut(s) 243
SchI GAGTC 2 cut(s) 362, 556
ScrFI CCNGG 2 cut(s) 182, 326
SfaNI GCATC 2 cut(s) 82, 106
SmlI CTYRAG 4 cut(s) 158, 313, 455, 544
SmoI CTYRAG 4 cut(s) 158, 313, 455, 544
SpeI ACTAGT 1 cut(s) 11
SphI GCATGC 1 cut(s) 30
Sse9I AATT 1 cut(s) 350
SspMI CTAG 3 cut(s) 12, 365, 590
StyD4I CCNGG 2 cut(s) 180, 324
TaaI ACNGT 1 cut(s) 414
TaiI ACGT 2 cut(s) 39, 52
TaqI TCGA 4 cut(s) 111, 126, 396, 435
TasI AATT 1 cut(s) 350
TfiI GAWTC 1 cut(s) 58
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 1 cut(s) 419
TseI GCWGC 5 cut(s) 318, 321, 331, 420, 677
TspDTI ATGAA 5 cut(s) 101, 290, 547, 789, 797
TspRI CASTG 1 cut(s) 419
Vha464I CTTAAG 1 cut(s) 158
XbaI TCTAGA 1 cut(s) 364
XceI RCATGY 3 cut(s) 30, 404, 684
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 3 cut(s) 12, 365, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.