RLG00000014376

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0007487
Physical Location & Seq
Forward (+)
52319865 .. 52323704
3840 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014376

Sequence Viewer

Length: 1323 bp
ATGATGGTGGTCCGATGGGCTGCGAAGACTCGAGATGGTCTCTCATCGGCGTCAAGCGGCGCTGGGCTGTTCGCTCTGGGCGAGGTCGGATGGGGACTGGTTGTTGGCTGGGGTCGAGGATATCTGGGTTCAGATCCGATCGGGGACAGTTTGGAAGTGATGGAGGGCTGTGATCGAGCACTCTCTGAGGGTGTGGTGAGTGATCCCGGGGGCTATGCTAGATCGGGATCGGATTCAGGCCAAGGAACTGTTGCGGCTGCTGGAACGGCGGCTCGGACGGCGAGTGTCTGCGGCGGCGGCGGCGGCGTGGCTGATGGATCGTGGCTGGAAAGATATAAAAACAGATTCAACTTAAGAGTTTCTCTGGCAATGAAGGAAACGAAAGCAAAAAGTATCCCAATGCAACTAACTAGTGCTTTGTGGGCATGCAAGAACGTTTTACTGCAACGTGTAGGGTATCAGACAGCAAATCAGGAGGACTGCATGAATGATGCAAAGTTGCTGTCCTTCGATGATGCCAGCCTCGAAACCAGTACCAATAAGCTCATTACCAAACTTAAGGAGGATGGAGGTCTTTCCCTGGATGTTTTGGAGAATGTGCTGACAAGTATGTCTGATTTAGGGCAAAAAACTGCTGACGCGATCTTGGATTTCAAGAGTAAGATGATTGATATGAAGGACGATAGCTATAAGCTTGTGGTGATGGAATACTTGAGCAGCAGCCTGGAGCTGCTGGACTTCTTTACTGAATTGGAAAAGTTTCTAGACTCGGCTCGTCGGAGCCATTCACATATCGAACATGCGATTGAACAGTGTAGCAGCTTGGGGAGTAAACATAGACCAGGAAGCGAGGAGGCCAAGATGATTTCCAATATATTGAGTAACATTATGGCGTCTAATGTCAATGACCACTCTGGTGAAAAGTTGGTCGAAAAAGCTCATTGTTTGCTTGAGAAGCAGAAAGAAATGTTTTCCAAATGTCGCATTGAACAAGAGAAGCTTGGGAAGGATAAGAAGAGTAATGTTTGGAGGAAGCTCTTGAGTCGTATATTCATGGTTGTCAAGTGGCTCGTTCTCGTTGTTCTAGGGGTGATATTGGCCTATACGCCCTATAATATAACATCTGATGGAGATGGTTTGTCTAATGTGGTTGGAGATTTGAAAATCAAAGCAGCATGTTTCCTTGATGTCTCTGTTATAACAGAAATAGTAGATTACTGGAATAGCTCCCACCTTGAAGTATGTCAAGATGATTTTGGGCTGAAGAATGAAGCAATGAACATAATACAGGAAGGAGCTTTGAAAGAAAGAAGATTTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

441

Amino Acids

48.0

Weight (kDa)

5.56

Isoelectric Point (pI)

38.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 169 - 368 2e-16 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1199
AasI GACNNNNNNGTC 1 cut(s) 610
AccII CGCG 1 cut(s) 641
AciI CCGC 8 cut(s) 57, 254, 269, 291, 294, 297, 300, 303
AclI AACGTT 1 cut(s) 435
AclWI GGATC 4 cut(s) 128, 197, 235, 325
AcuI CTGAAG 1 cut(s) 1283
AcyI GRCGYC 2 cut(s) 50, 893
AfaI GTAC 1 cut(s) 535
AflII CTTAAG 2 cut(s) 352, 557
AflIII ACRYGT 1 cut(s) 448
AgsI TTSAA 7 cut(s) 349, 655, 809, 989, 1162, 1238, 1303
AhlI ACTAGT 1 cut(s) 410
AjnI CCWGG 3 cut(s) 579, 723, 841
AjuI GAANNNNNNNTTGG 1 cut(s) 1299
AleI CACNNNNGTG 1 cut(s) 915
Alw21I GWGCWC 1 cut(s) 181
Alw26I GTCTC 2 cut(s) 44, 1195
AlwI GGATC 4 cut(s) 128, 197, 235, 325
Ama87I CYCGRG 2 cut(s) 30, 206
AoxI GGCC 3 cut(s) 238, 855, 1098
ApeKI GCWGC 7 cut(s) 20, 257, 717, 720, 730, 819, 1172
ArsI GACNNNNNNTTYG 2 cut(s) 488, 520
Asp700I GAANNNNTTC 1 cut(s) 759
AspLEI GCGC 1 cut(s) 62
AspS9I GGNCC 1 cut(s) 10
AsuC2I CCSGG 2 cut(s) 207, 208
AsuHPI GGTGA 4 cut(s) 208, 712, 929, 1102
AvaI CYCGRG 2 cut(s) 30, 206
AvaII GGWCC 1 cut(s) 10
BbsI GAAGAC 1 cut(s) 32
Bbv12I GWGCWC 1 cut(s) 181
BbvI GCAGC 7 cut(s) 7, 244, 717, 729, 732, 831, 1184
BccI CCATC 9 cut(s) 9, 29, 84, 154, 308, 560, 697, 1121, 1127
BceAI ACGGC 2 cut(s) 282, 294
BciT130I CCWGG 3 cut(s) 581, 725, 843
BciVI GTATCC 1 cut(s) 404
BcnI CCSGG 2 cut(s) 207, 208
BcoDI GTCTC 2 cut(s) 44, 1195
BcuI ACTAGT 1 cut(s) 410
BfaI CTAG 4 cut(s) 219, 411, 764, 1085
BfoI RGCGCY 1 cut(s) 63
BfrI CTTAAG 2 cut(s) 352, 557
BfuI GTATCC 1 cut(s) 404
Bme1390I CCNGG 5 cut(s) 207, 208, 581, 725, 843
Bme18I GGWCC 1 cut(s) 10
BmeT110I CYCGRG 2 cut(s) 30, 206
BmgT120I GGNCC 1 cut(s) 10
BmiI GGNNCC 1 cut(s) 782
BmrFI CCNGG 5 cut(s) 207, 208, 581, 725, 843
BmsI GCATC 2 cut(s) 481, 505
BpiI GAAGAC 1 cut(s) 32
BplI GAGNNNNNCTC 2 cut(s) 24, 56
BpmI CTGGAG 1 cut(s) 746
BpuEI CTTGAG 3 cut(s) 733, 971, 1060
BpuMI CCSGG 2 cut(s) 207, 208
BsaBI GATNNNNATC 1 cut(s) 226
BsaHI GRCGYC 2 cut(s) 50, 893
BsaI GGTCTC 1 cut(s) 44
BsaJI CCNNGG 4 cut(s) 206, 207, 241, 579
Bse1I ACTGG 3 cut(s) 102, 531, 1223
Bse3DI GCAATG 2 cut(s) 375, 1281
Bse8I GATNNNNATC 1 cut(s) 226
BseBI CCWGG 3 cut(s) 581, 725, 843
BseDI CCNNGG 4 cut(s) 206, 207, 241, 579
BseGI GGATG 3 cut(s) 95, 571, 589
BseJI GATNNNNATC 1 cut(s) 226
BseMI GCAATG 2 cut(s) 375, 1281
BseMII CTCAG 1 cut(s) 177
BseNI ACTGG 3 cut(s) 102, 531, 1223
BseRI GAGGAG 1 cut(s) 866
BseXI GCAGC 7 cut(s) 7, 244, 717, 729, 732, 831, 1184
BseYI CCCAGC 2 cut(s) 62, 108
Bsh1236I CGCG 1 cut(s) 641
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 3 cut(s) 240, 857, 1100
BsiEI CGRYCG 1 cut(s) 141
BsiHKAI GWGCWC 1 cut(s) 181
BsiHKCI CYCGRG 2 cut(s) 30, 206
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 2 cut(s) 108, 158
BsmAI GTCTC 2 cut(s) 44, 1195
BsmFI GGGAC 2 cut(s) 108, 158
BsnI GGCC 3 cut(s) 240, 857, 1100
Bso31I GGTCTC 1 cut(s) 44
BsoBI CYCGRG 2 cut(s) 30, 206
Bsp1286I GDGCHC 1 cut(s) 181
Bsp143I GATC 8 cut(s) 133, 138, 172, 202, 221, 227, 317, 642
BspACI CCGC 8 cut(s) 57, 254, 269, 291, 294, 297, 300, 303
BspANI GGCC 3 cut(s) 240, 857, 1100
BspCNI CTCAG 1 cut(s) 178
BspFNI CGCG 1 cut(s) 641
BspLI GGNNCC 1 cut(s) 782
BspPI GGATC 4 cut(s) 128, 197, 235, 325
BspTI CTTAAG 2 cut(s) 352, 557
BspTNI GGTCTC 1 cut(s) 44
BsrDI GCAATG 2 cut(s) 375, 1281
BsrI ACTGG 3 cut(s) 102, 531, 1223
BssECI CCNNGG 4 cut(s) 206, 207, 241, 579
BssMI GATC 8 cut(s) 133, 138, 172, 202, 221, 227, 317, 642
BssNI GRCGYC 2 cut(s) 50, 893
BssT1I CCWWGG 1 cut(s) 241
Bst2UI CCWGG 3 cut(s) 581, 725, 843
Bst4CI ACNGT 3 cut(s) 149, 250, 813
Bst6I CTCTTC 1 cut(s) 1010
BstACI GRCGYC 2 cut(s) 50, 893
BstAFI CTTAAG 2 cut(s) 352, 557
BstC8I GCNNGC 2 cut(s) 427, 520
BstDEI CTNAG 1 cut(s) 186
BstF5I GGATG 3 cut(s) 95, 571, 589
BstFNI CGCG 1 cut(s) 641
BstH2I RGCGCY 1 cut(s) 63
BstHHI GCGC 1 cut(s) 62
BstKTI GATC 8 cut(s) 136, 141, 175, 205, 224, 230, 320, 645
BstMAI GTCTC 2 cut(s) 44, 1195
BstMBI GATC 8 cut(s) 133, 138, 172, 202, 221, 227, 317, 642
BstMCI CGRYCG 1 cut(s) 141
BstMWI GCNNNNNNNGC 7 cut(s) 266, 278, 297, 300, 303, 422, 955
BstNI CCWGG 3 cut(s) 581, 725, 843
BstNSI RCATGY 3 cut(s) 429, 803, 1179
BstSCI CCNGG 5 cut(s) 205, 206, 579, 723, 841
BstUI CGCG 1 cut(s) 641
BstV1I GCAGC 7 cut(s) 7, 244, 717, 729, 732, 831, 1184
BstV2I GAAGAC 1 cut(s) 32
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BsuI GTATCC 1 cut(s) 404
BsuRI GGCC 3 cut(s) 240, 857, 1100
BtsCI GGATG 3 cut(s) 95, 571, 589
BtsIMutI CAGTG 1 cut(s) 818
Cac8I GCNNGC 2 cut(s) 427, 520
CfoI GCGC 1 cut(s) 62
Cfr13I GGNCC 1 cut(s) 10
Cfr9I CCCGGG 1 cut(s) 206
CseI GACGC 3 cut(s) 39, 647, 882
Csp6I GTAC 1 cut(s) 534
CviAII CATG 5 cut(s) 426, 484, 800, 1054, 1176
CviQI GTAC 1 cut(s) 534
DdeI CTNAG 1 cut(s) 186
DpnI GATC 8 cut(s) 135, 140, 174, 204, 223, 229, 319, 644
DpnII GATC 8 cut(s) 133, 138, 172, 202, 221, 227, 317, 642
DrdI GACNNNNNNGTC 1 cut(s) 610
DseDI GACNNNNNNGTC 1 cut(s) 610
Eam1104I CTCTTC 1 cut(s) 1010
EarI CTCTTC 1 cut(s) 1010
Eco130I CCWWGG 1 cut(s) 241
Eco31I GGTCTC 1 cut(s) 44
Eco32I GATATC 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 10
Eco57I CTGAAG 1 cut(s) 1283
Eco88I CYCGRG 2 cut(s) 30, 206
EcoRII CCWGG 3 cut(s) 579, 723, 841
EcoRV GATATC 1 cut(s) 122
EcoT14I CCWWGG 1 cut(s) 241
ErhI CCWWGG 1 cut(s) 241
FaeI CATG 5 cut(s) 429, 487, 803, 1057, 1179
FalI AAGNNNNNCTT 2 cut(s) 984, 1016
FaqI GGGAC 2 cut(s) 108, 158
FatI CATG 5 cut(s) 425, 483, 799, 1053, 1175
FokI GGATG 3 cut(s) 102, 578, 596
FspBI CTAG 4 cut(s) 219, 411, 764, 1085
GlaI GCGC 1 cut(s) 61
GsaI CCCAGC 2 cut(s) 66, 112
GsuI CTGGAG 1 cut(s) 746
HaeII RGCGCY 1 cut(s) 63
HaeIII GGCC 3 cut(s) 240, 857, 1100
HapII CCGG 1 cut(s) 207
HgaI GACGC 3 cut(s) 39, 647, 882
HhaI GCGC 1 cut(s) 62
Hin1I GRCGYC 2 cut(s) 50, 893
Hin1II CATG 5 cut(s) 429, 487, 803, 1057, 1179
Hin6I GCGC 1 cut(s) 60
HinP1I GCGC 1 cut(s) 60
HindIII AAGCTT 2 cut(s) 692, 998
HinfI GANTC 5 cut(s) 28, 233, 345, 767, 1042
HpaII CCGG 1 cut(s) 207
HphI GGTGA 4 cut(s) 208, 712, 929, 1102
Hpy166II GTNNAC 1 cut(s) 833
Hpy188III TCNNGA 7 cut(s) 32, 225, 473, 655, 764, 1039, 1247
Hpy8I GTNNAC 1 cut(s) 833
Hpy99I CGWCG 1 cut(s) 780
HpyAV CCTTC 5 cut(s) 367, 517, 670, 1000, 1286
HpyCH4III ACNGT 3 cut(s) 149, 250, 813
HpyCH4IV ACGT 2 cut(s) 435, 448
HpyCH4V TGCA 5 cut(s) 403, 429, 445, 483, 494
HpyF10VI GCNNNNNNNGC 7 cut(s) 266, 278, 297, 300, 303, 422, 955
HpyF3I CTNAG 1 cut(s) 186
HpySE526I ACGT 2 cut(s) 435, 448
Hsp92I GRCGYC 2 cut(s) 50, 893
Hsp92II CATG 5 cut(s) 429, 487, 803, 1057, 1179
HspAI GCGC 1 cut(s) 60
Kzo9I GATC 8 cut(s) 133, 138, 172, 202, 221, 227, 317, 642
LmnI GCTCC 4 cut(s) 727, 780, 1232, 1295
Lsp1109I GCAGC 7 cut(s) 7, 244, 717, 729, 732, 831, 1184
LweI GCATC 2 cut(s) 481, 505
MaeI CTAG 4 cut(s) 219, 411, 764, 1085
MaeII ACGT 2 cut(s) 435, 448
MaeIII GTNAC 1 cut(s) 881
MalI GATC 8 cut(s) 135, 140, 174, 204, 223, 229, 319, 644
MboI GATC 8 cut(s) 133, 138, 172, 202, 221, 227, 317, 642
MboII GAAGA 4 cut(s) 37, 1027, 1276, 1323
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 1 cut(s) 181
MluCI AATT 1 cut(s) 749
MlyI GAGTC 3 cut(s) 22, 761, 1051
MmeI TCCRAC 3 cut(s) 67, 758, 1132
MroXI GAANNNNTTC 1 cut(s) 759
MseI TTAA 2 cut(s) 353, 558
MslI CAYNNNNRTG 1 cut(s) 915
MspCI CTTAAG 2 cut(s) 352, 557
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 5 cut(s) 207, 208, 581, 725, 843
MvaI CCWGG 3 cut(s) 581, 725, 843
MvnI CGCG 1 cut(s) 641
MwoI GCNNNNNNNGC 7 cut(s) 266, 278, 297, 300, 303, 422, 955
NciI CCSGG 2 cut(s) 207, 208
NdeII GATC 8 cut(s) 133, 138, 172, 202, 221, 227, 317, 642
NlaIII CATG 5 cut(s) 429, 487, 803, 1057, 1179
NlaIV GGNNCC 1 cut(s) 782
NmeAIII GCCGAG 1 cut(s) 749
NspI RCATGY 3 cut(s) 429, 803, 1179
OliI CACNNNNGTG 1 cut(s) 915
PaeI GCATGC 1 cut(s) 429
PaeR7I CTCGAG 1 cut(s) 30
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PdmI GAANNNNTTC 1 cut(s) 759
PfeI GAWTC 2 cut(s) 233, 345
Ple19I CGATCG 1 cut(s) 141
PleI GAGTC 3 cut(s) 22, 761, 1050
PpsI GAGTC 3 cut(s) 22, 761, 1050
PsiI TTATAA 1 cut(s) 1199
Psp1406I AACGTT 1 cut(s) 435
Psp6I CCWGG 3 cut(s) 579, 723, 841
PspFI CCCAGC 2 cut(s) 62, 108
PspGI CCWGG 3 cut(s) 579, 723, 841
PspN4I GGNNCC 1 cut(s) 782
PspPI GGNCC 1 cut(s) 10
PsuI RGATCY 1 cut(s) 133
PvuI CGATCG 1 cut(s) 141
RsaI GTAC 1 cut(s) 535
RsaNI GTAC 1 cut(s) 534
RseI CAYNNNNRTG 1 cut(s) 915
SaqAI TTAA 2 cut(s) 353, 558
Sau3AI GATC 8 cut(s) 133, 138, 172, 202, 221, 227, 317, 642
Sau96I GGNCC 1 cut(s) 10
SchI GAGTC 3 cut(s) 22, 761, 1051
ScrFI CCNGG 5 cut(s) 207, 208, 581, 725, 843
SduI GDGCHC 1 cut(s) 181
SfaNI GCATC 2 cut(s) 481, 505
Sfr274I CTCGAG 1 cut(s) 30
SinI GGWCC 1 cut(s) 10
SlaI CTCGAG 1 cut(s) 30
SmaI CCCGGG 1 cut(s) 208
SmiMI CAYNNNNRTG 1 cut(s) 915
SmlI CTYRAG 6 cut(s) 30, 352, 557, 712, 950, 1039
SmoI CTYRAG 6 cut(s) 30, 352, 557, 712, 950, 1039
SpeI ACTAGT 1 cut(s) 410
SphI GCATGC 1 cut(s) 429
Sse9I AATT 1 cut(s) 749
SsiI CCGC 8 cut(s) 57, 254, 269, 291, 294, 297, 300, 303
SspMI CTAG 4 cut(s) 219, 411, 764, 1085
StyD4I CCNGG 5 cut(s) 205, 206, 579, 723, 841
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 3 cut(s) 149, 250, 813
TaiI ACGT 2 cut(s) 438, 451
TaqI TCGA 7 cut(s) 31, 115, 175, 510, 525, 795, 930
TasI AATT 1 cut(s) 749
TauI GCSGC 8 cut(s) 60, 257, 272, 294, 297, 300, 303, 306
TfiI GAWTC 2 cut(s) 233, 345
Tru1I TTAA 2 cut(s) 353, 558
Tru9I TTAA 2 cut(s) 353, 558
TscAI CASTG 1 cut(s) 818
TseI GCWGC 7 cut(s) 20, 257, 717, 720, 730, 819, 1172
TspDTI ATGAA 6 cut(s) 386, 500, 689, 1042, 1284, 1292
TspMI CCCGGG 1 cut(s) 206
TspRI CASTG 1 cut(s) 818
Vha464I CTTAAG 2 cut(s) 352, 557
VpaK11BI GGWCC 1 cut(s) 10
XbaI TCTAGA 1 cut(s) 763
XceI RCATGY 3 cut(s) 429, 803, 1179
XhoI CTCGAG 1 cut(s) 30
XmaI CCCGGG 1 cut(s) 206
XmnI GAANNNNTTC 1 cut(s) 759
XspI CTAG 4 cut(s) 219, 411, 764, 1085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.