Rh6BG116900

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0007487
Physical Location & Seq
Reverse (-)
18068862 .. 18073613
4752 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG116900.1

Sequence Viewer

Length: 1287 bp
ATGAAGGAAACGAAAGCAAAAAGTATTCCATTGCAACTAACTAGTGATTTGTGGGCATGCAAGAACGTTTTAATGCAACATGTAGGGTATCAGACAGCAAATCAGGAGGACTGCAAGAATGATGCAAAGTTGCTGTCCTACGATGATGCCAGCCTCGAAACCAGTACCAATGAGCTCATTACCAAACTTATGGAGGATGGAGGTCTTTCCCTGGATGTTTTGGAGAATGTGCTGACAAGTATGTCTGATTTAGGGCAAAAAACCGCTGACGCGATCTTGGATTTCAAGAGTAAGATGATCGCTATGAAGGACGATAGCTATAAGCTTGTGGTGATGGAATACATGAGCAGCAGCCTGGAGCTGCTGGACTTCTTTACTGAACTGGAAAAGTTTCTAGACGCGGCTCGTCGGAGCCATTCACATATCGAACATGCGATTGAACAGTGTAGCAGCTTGGGGAGTAAACATGGAGTAGGAAGGGAGGAGGCCAAGATGATTTCCAATATATTGAGTAACATTATGGCATCTAATGTCAATGACCACTCTGGTGAAAAGTTGGTCGAAAAAGTTCATTGTTTGCTTGAGAAGCAGAAAGAAATGTTTTCCAAATGTCGCATTGAACAAGAGAAGCTTGGGAAGGATCAGAAGAGTAATGTTTGGAGGAAGCTCTTGAGTCGTATATTCATGGTTGCCAAGTGGCTCGTTCTCGTTGTTCTAGGGGTGATATTGGCCTATACGGCCTATAATATAACATCTGATGGAGATGGTTTGTCTAATGTGTTTGGAGATTTGAAAATCAAAGCAGCTTGTTTCCTTGATGTCCCTGTTATAATAGAAATAGTAGATTACTGGAATAGCTCCCACCTTGAAGTATGTCAAGATGATTTTGGGCTGAAGATTGAAGCAATGAACATAATACAGGAAGGAGCTTTACATTCCATAAGAGAATTGGGGGAAATAAAAGATCTCATCGGTAAGGTAGTAAATTCCATTGAATCTCTCCTTGCTCTAAGTACAAACTTTGCAAATGGAGGAAATGTAGAAGTGGCAATCAAGGATATTAAGCTTCACCTGAAGTTTTTGGATAAAATTGAGGTTTTGGAGGAGAAAACAGGGGAATGCAGCTCTAGAATCACTACTACACAAAAGGCTTCACACGACGGTTTAAAACTGTCGTCTCACGTTTTGCATTTCAATGGTCTATCGACGCGTCATCTGATGAAACGCTGTCAGACGACGGTTTTCAACCGTCATATATCGATCATTGGGTCAACGGTTTTCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

428

Amino Acids

47.83

Weight (kDa)

5.87

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 48 - 382 2.1e-24 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 830
AasI GACNNNNNNGTC 1 cut(s) 241
AccII CGCG 3 cut(s) 272, 401, 1210
AciI CCGC 2 cut(s) 264, 401
AclI AACGTT 1 cut(s) 66
AclWI GGATC 1 cut(s) 648
AcsI RAATTY 1 cut(s) 985
AcuI CTGAAG 2 cut(s) 914, 1094
AfaI GTAC 2 cut(s) 166, 1015
AflIII ACRYGT 2 cut(s) 79, 1208
AgsI TTSAA 9 cut(s) 286, 440, 620, 793, 869, 902, 995, 1195, 1246
AhlI ACTAGT 1 cut(s) 41
AjnI CCWGG 2 cut(s) 210, 354
AleI CACNNNNGTG 1 cut(s) 546
Alw21I GWGCWC 1 cut(s) 177
Alw26I GTCTC 1 cut(s) 1182
AlwI GGATC 1 cut(s) 648
AoxI GGCC 3 cut(s) 486, 729, 738
ApeKI GCWGC 6 cut(s) 348, 351, 361, 450, 803, 1122
ApoI RAATTY 1 cut(s) 985
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
Asp700I GAANNNNTTC 2 cut(s) 390, 567
AsuHPI GGTGA 4 cut(s) 343, 560, 733, 1061
BanII GRGCYC 1 cut(s) 177
BarI GAAGNNNNNNTAC 2 cut(s) 915, 947
Bbv12I GWGCWC 1 cut(s) 177
BbvI GCAGC 6 cut(s) 348, 360, 363, 462, 815, 1134
BccI CCATC 4 cut(s) 191, 328, 752, 758
BceAI ACGGC 1 cut(s) 753
BciT130I CCWGG 2 cut(s) 212, 356
BcoDI GTCTC 1 cut(s) 1182
BcuI ACTAGT 1 cut(s) 41
BfaI CTAG 4 cut(s) 42, 395, 716, 1128
BglI GCCNNNNNGGC 1 cut(s) 737
BglII AGATCT 1 cut(s) 964
BisI GCNGC 7 cut(s) 349, 352, 362, 402, 451, 804, 1123
BlsI GCNGC 7 cut(s) 350, 353, 363, 403, 452, 805, 1124
Bme1390I CCNGG 2 cut(s) 212, 356
BmiI GGNNCC 1 cut(s) 413
BmrFI CCNGG 2 cut(s) 212, 356
BmsI GCATC 3 cut(s) 112, 136, 533
BpmI CTGGAG 1 cut(s) 377
BpuEI CTTGAG 2 cut(s) 602, 691
Bsa29I ATCGAT 1 cut(s) 1259
BsaJI CCNNGG 1 cut(s) 210
BsaXI ACNNNNNCTCC 2 cut(s) 1023, 1053
Bse1I ACTGG 3 cut(s) 162, 387, 854
Bse3DI GCAATG 2 cut(s) 29, 912
BseBI CCWGG 2 cut(s) 212, 356
BseCI ATCGAT 1 cut(s) 1259
BseDI CCNNGG 1 cut(s) 210
BseGI GGATG 2 cut(s) 202, 220
BseMI GCAATG 2 cut(s) 29, 912
BseNI ACTGG 3 cut(s) 162, 387, 854
BseRI GAGGAG 2 cut(s) 497, 1118
BseXI GCAGC 6 cut(s) 348, 360, 363, 462, 815, 1134
Bsh1236I CGCG 3 cut(s) 272, 401, 1210
BshFI GGCC 3 cut(s) 488, 731, 740
BshVI ATCGAT 1 cut(s) 1259
BsiHKAI GWGCWC 1 cut(s) 177
BslFI GGGAC 1 cut(s) 806
BsmAI GTCTC 1 cut(s) 1182
BsmBI CGTCTC 1 cut(s) 1182
BsmFI GGGAC 1 cut(s) 806
BsmI GAATGC 1 cut(s) 1124
BsnI GGCC 3 cut(s) 488, 731, 740
Bsp1286I GDGCHC 1 cut(s) 177
Bsp143I GATC 5 cut(s) 273, 297, 640, 964, 1260
BspACI CCGC 2 cut(s) 264, 401
BspANI GGCC 3 cut(s) 488, 731, 740
BspDI ATCGAT 1 cut(s) 1259
BspFNI CGCG 3 cut(s) 272, 401, 1210
BspLI GGNNCC 1 cut(s) 413
BspPI GGATC 1 cut(s) 648
BsrDI GCAATG 2 cut(s) 29, 912
BsrI ACTGG 3 cut(s) 162, 387, 854
BssECI CCNNGG 1 cut(s) 210
BssMI GATC 5 cut(s) 273, 297, 640, 964, 1260
Bst2UI CCWGG 2 cut(s) 212, 356
Bst4CI ACNGT 6 cut(s) 444, 1163, 1173, 1240, 1250, 1276
Bst6I CTCTTC 1 cut(s) 641
BstC8I GCNNGC 2 cut(s) 58, 151
BstDEI CTNAG 1 cut(s) 1010
BstF5I GGATG 2 cut(s) 202, 220
BstFNI CGCG 3 cut(s) 272, 401, 1210
BstKTI GATC 5 cut(s) 276, 300, 643, 967, 1263
BstMAI GTCTC 1 cut(s) 1182
BstMBI GATC 5 cut(s) 273, 297, 640, 964, 1260
BstMWI GCNNNNNNNGC 2 cut(s) 586, 737
BstNI CCWGG 2 cut(s) 212, 356
BstNSI RCATGY 3 cut(s) 60, 83, 434
BstSCI CCNGG 2 cut(s) 210, 354
BstUI CGCG 3 cut(s) 272, 401, 1210
BstV1I GCAGC 6 cut(s) 348, 360, 363, 462, 815, 1134
BstX2I RGATCY 1 cut(s) 964
BstXI CCANNNNNNTGG 1 cut(s) 190
BstYI RGATCY 1 cut(s) 964
Bsu15I ATCGAT 1 cut(s) 1259
BsuRI GGCC 3 cut(s) 488, 731, 740
BsuTUI ATCGAT 1 cut(s) 1259
BtsCI GGATG 2 cut(s) 202, 220
BtsIMutI CAGTG 1 cut(s) 449
Cac8I GCNNGC 2 cut(s) 58, 151
ClaI ATCGAT 1 cut(s) 1259
CseI GACGC 4 cut(s) 278, 407, 1199, 1216
Csp6I GTAC 2 cut(s) 165, 1014
CviAII CATG 6 cut(s) 57, 80, 343, 431, 467, 685
CviQI GTAC 2 cut(s) 165, 1014
DdeI CTNAG 1 cut(s) 1010
DpnI GATC 5 cut(s) 275, 299, 642, 966, 1262
DpnII GATC 5 cut(s) 273, 297, 640, 964, 1260
DraI TTTAAA 1 cut(s) 1167
DrdI GACNNNNNNGTC 1 cut(s) 241
DseDI GACNNNNNNGTC 1 cut(s) 241
Eam1104I CTCTTC 1 cut(s) 641
EarI CTCTTC 1 cut(s) 641
Ecl136II GAGCTC 1 cut(s) 175
Eco24I GRGCYC 1 cut(s) 177
Eco53kI GAGCTC 1 cut(s) 175
Eco57I CTGAAG 2 cut(s) 914, 1094
EcoICRI GAGCTC 1 cut(s) 175
EcoRII CCWGG 2 cut(s) 210, 354
EcoT38I GRGCYC 1 cut(s) 177
Esp3I CGTCTC 1 cut(s) 1182
FaeI CATG 6 cut(s) 60, 83, 346, 434, 470, 688
FalI AAGNNNNNCTT 2 cut(s) 615, 647
FaqI GGGAC 1 cut(s) 806
FatI CATG 6 cut(s) 56, 79, 342, 430, 466, 684
Fnu4HI GCNGC 7 cut(s) 349, 352, 362, 402, 451, 804, 1123
FokI GGATG 2 cut(s) 209, 227
FriOI GRGCYC 1 cut(s) 177
Fsp4HI GCNGC 7 cut(s) 349, 352, 362, 402, 451, 804, 1123
FspBI CTAG 4 cut(s) 42, 395, 716, 1128
GluI GCNGC 7 cut(s) 349, 352, 362, 402, 451, 804, 1123
GsuI CTGGAG 1 cut(s) 377
HaeIII GGCC 3 cut(s) 488, 731, 740
HgaI GACGC 4 cut(s) 278, 407, 1199, 1216
Hin1II CATG 6 cut(s) 60, 83, 346, 434, 470, 688
HincII GTYRAC 1 cut(s) 1272
HindII GTYRAC 1 cut(s) 1272
HindIII AAGCTT 3 cut(s) 323, 629, 1064
HinfI GANTC 3 cut(s) 673, 995, 1131
HphI GGTGA 4 cut(s) 343, 560, 733, 1061
Hpy166II GTNNAC 2 cut(s) 464, 1272
Hpy188I TCNGA 7 cut(s) 93, 247, 411, 645, 757, 1218, 1233
Hpy188III TCNNGA 6 cut(s) 104, 286, 395, 670, 878, 1128
Hpy8I GTNNAC 2 cut(s) 464, 1272
Hpy99I CGWCG 4 cut(s) 411, 1163, 1210, 1240
HpyAV CCTTC 4 cut(s) 301, 471, 631, 917
HpyCH4III ACNGT 6 cut(s) 444, 1163, 1173, 1240, 1250, 1276
HpyCH4IV ACGT 2 cut(s) 66, 1182
HpyCH4V TGCA 8 cut(s) 34, 60, 76, 114, 125, 1025, 1122, 1189
HpyF10VI GCNNNNNNNGC 2 cut(s) 586, 737
HpyF3I CTNAG 1 cut(s) 1010
HpySE526I ACGT 2 cut(s) 66, 1182
Hsp92II CATG 6 cut(s) 60, 83, 346, 434, 470, 688
Kzo9I GATC 5 cut(s) 273, 297, 640, 964, 1260
LmnI GCTCC 4 cut(s) 358, 411, 863, 926
Lsp1109I GCAGC 6 cut(s) 348, 360, 363, 462, 815, 1134
LweI GCATC 3 cut(s) 112, 136, 533
MaeI CTAG 4 cut(s) 42, 395, 716, 1128
MaeII ACGT 2 cut(s) 66, 1182
MaeIII GTNAC 1 cut(s) 512
MalI GATC 5 cut(s) 275, 299, 642, 966, 1262
MboI GATC 5 cut(s) 273, 297, 640, 964, 1260
MboII GAAGA 2 cut(s) 658, 907
MflI RGATCY 1 cut(s) 964
MhlI GDGCHC 1 cut(s) 177
MluCI AATT 3 cut(s) 947, 985, 1089
MluI ACGCGT 1 cut(s) 1208
MlyI GAGTC 1 cut(s) 682
MmeI TCCRAC 1 cut(s) 389
MroXI GAANNNNTTC 2 cut(s) 390, 567
MseI TTAA 3 cut(s) 71, 1062, 1166
MslI CAYNNNNRTG 2 cut(s) 546, 1194
MspA1I CMGCKG 1 cut(s) 266
MspR9I CCNGG 2 cut(s) 212, 356
Mva1269I GAATGC 1 cut(s) 1124
MvaI CCWGG 2 cut(s) 212, 356
MvnI CGCG 3 cut(s) 272, 401, 1210
MwoI GCNNNNNNNGC 2 cut(s) 586, 737
NdeII GATC 5 cut(s) 273, 297, 640, 964, 1260
NlaIII CATG 6 cut(s) 60, 83, 346, 434, 470, 688
NlaIV GGNNCC 1 cut(s) 413
NspI RCATGY 3 cut(s) 60, 83, 434
OliI CACNNNNGTG 1 cut(s) 546
PaeI GCATGC 1 cut(s) 60
PciI ACATGT 1 cut(s) 79
PctI GAATGC 1 cut(s) 1124
PdmI GAANNNNTTC 2 cut(s) 390, 567
PfeI GAWTC 2 cut(s) 995, 1131
PkrI GCNGC 7 cut(s) 350, 353, 363, 403, 452, 805, 1124
PleI GAGTC 1 cut(s) 681
PpsI GAGTC 1 cut(s) 681
PscI ACATGT 1 cut(s) 79
PsiI TTATAA 1 cut(s) 830
Psp124BI GAGCTC 1 cut(s) 177
Psp1406I AACGTT 1 cut(s) 66
Psp6I CCWGG 2 cut(s) 210, 354
PspGI CCWGG 2 cut(s) 210, 354
PspN4I GGNNCC 1 cut(s) 413
PsuI RGATCY 1 cut(s) 964
RsaI GTAC 2 cut(s) 166, 1015
RsaNI GTAC 2 cut(s) 165, 1014
RseI CAYNNNNRTG 2 cut(s) 546, 1194
SacI GAGCTC 1 cut(s) 177
SaqAI TTAA 3 cut(s) 71, 1062, 1166
SatI GCNGC 7 cut(s) 349, 352, 362, 402, 451, 804, 1123
Sau3AI GATC 5 cut(s) 273, 297, 640, 964, 1260
SchI GAGTC 1 cut(s) 682
ScrFI CCNGG 2 cut(s) 212, 356
SduI GDGCHC 1 cut(s) 177
SfaNI GCATC 3 cut(s) 112, 136, 533
SfiI GGCCNNNNNGGCC 1 cut(s) 737
SmiMI CAYNNNNRTG 2 cut(s) 546, 1194
SmlI CTYRAG 2 cut(s) 581, 670
SmoI CTYRAG 2 cut(s) 581, 670
SpeI ACTAGT 1 cut(s) 41
SphI GCATGC 1 cut(s) 60
Sse9I AATT 3 cut(s) 947, 985, 1089
SsiI CCGC 2 cut(s) 264, 401
SspMI CTAG 4 cut(s) 42, 395, 716, 1128
SstI GAGCTC 1 cut(s) 177
StyD4I CCNGG 2 cut(s) 210, 354
TaaI ACNGT 6 cut(s) 444, 1163, 1173, 1240, 1250, 1276
TaiI ACGT 2 cut(s) 69, 1185
TaqI TCGA 5 cut(s) 156, 426, 561, 1205, 1259
TasI AATT 3 cut(s) 947, 985, 1089
TatI WGTACW 1 cut(s) 1013
TauI GCSGC 1 cut(s) 404
TfiI GAWTC 2 cut(s) 995, 1131
Tru1I TTAA 3 cut(s) 71, 1062, 1166
Tru9I TTAA 3 cut(s) 71, 1062, 1166
TscAI CASTG 1 cut(s) 449
TseI GCWGC 6 cut(s) 348, 351, 361, 450, 803, 1122
TspDTI ATGAA 6 cut(s) 17, 320, 560, 673, 923, 1235
TspRI CASTG 1 cut(s) 449
XapI RAATTY 1 cut(s) 985
XbaI TCTAGA 2 cut(s) 394, 1127
XceI RCATGY 3 cut(s) 60, 83, 434
XcmI CCANNNNNNNNNTGG 1 cut(s) 946
XmnI GAANNNNTTC 2 cut(s) 390, 567
XspI CTAG 4 cut(s) 42, 395, 716, 1128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.