FvH4_2g11500

Hemiasterlin resistant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004697
Physical Location & Seq
Reverse (-)
10063125 .. 10064619
1495 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g11500.t2

Sequence Viewer

Length: 384 bp
ATGAGTAGTTTTCACAGTGAAGGTCGATCTGCACGTCCAGCTCCTCGTCGTGCACCTGCACCTGCACCAGTTCGCCAGGCTCCTGCTCCAGCTCCTGCTCAGAGTGGAAGCGGTGGGGGTATTGGATCAGTCATTGCTGAAGGAATGGCTTTCGGCGCTGTGGATTCTTTGTTTGGGCCTCGTACTATCAAGCATGAAACTGTAGTCTCTGCTGCTCCAGCACCTACTGCTAGCAGTATGGGTGGTTCTGATGCTTGCAGTGTTCACTCCAAGGCATTCCAAGATTGTATCAGCAACTTTGGAAGTGATATCAGCAAGTGCCAGTTCTACATGGATATGTTGTCTGACTGCAAGAAGAACAACTCTGACATGCTCAGTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CHCH

Protein Analysis

128

Amino Acids

12.95

Weight (kDa)

7.7

Isoelectric Point (pI)

67.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHCH PF06747 86 - 120 6.7e-09 CHCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 64, 70
Acc36I ACCTGC 2 cut(s) 64, 70
AciI CCGC 1 cut(s) 111
AclWI GGATC 1 cut(s) 133
AcuI CTGAAG 1 cut(s) 159
AfaI GTAC 1 cut(s) 184
AjiI CACGTC 1 cut(s) 35
AjnI CCWGG 1 cut(s) 75
AluBI AGCT 2 cut(s) 41, 92
AluI AGCT 2 cut(s) 41, 92
Alw21I GWGCWC 1 cut(s) 55
Alw26I GTCTC 1 cut(s) 211
Alw44I GTGCAC 1 cut(s) 51
AlwI GGATC 1 cut(s) 133
AlwNI CAGNNNCTG 1 cut(s) 95
AoxI GGCC 1 cut(s) 176
ApaLI GTGCAC 1 cut(s) 51
ApeKI GCWGC 1 cut(s) 212
AspLEI GCGC 1 cut(s) 158
AspS9I GGNCC 1 cut(s) 176
AsuNHI GCTAGC 1 cut(s) 230
BaeGI GKGCMC 1 cut(s) 55
Bbv12I GWGCWC 1 cut(s) 55
BbvI GCAGC 1 cut(s) 199
BciT130I CCWGG 1 cut(s) 77
BcoDI GTCTC 1 cut(s) 211
BfaI CTAG 1 cut(s) 231
BfmI CTRYAG 1 cut(s) 201
BfoI RGCGCY 1 cut(s) 159
BfuAI ACCTGC 2 cut(s) 64, 70
BisI GCNGC 1 cut(s) 213
BlsI GCNGC 1 cut(s) 214
Bme1390I CCNGG 1 cut(s) 77
BmgBI CACGTC 1 cut(s) 35
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 81
BmrFI CCNGG 1 cut(s) 77
BmsI GCATC 1 cut(s) 241
BmtI GCTAGC 1 cut(s) 234
BpmI CTGGAG 2 cut(s) 72, 201
BsaJI CCNNGG 1 cut(s) 270
Bse1I ACTGG 2 cut(s) 68, 322
Bse3DI GCAATG 1 cut(s) 132
BseBI CCWGG 1 cut(s) 77
BseDI CCNNGG 1 cut(s) 270
BseMI GCAATG 1 cut(s) 132
BseMII CTCAG 1 cut(s) 113
BseNI ACTGG 2 cut(s) 68, 322
BseRI GAGGAG 1 cut(s) 33
BseSI GKGCMC 1 cut(s) 55
BseXI GCAGC 1 cut(s) 199
BsgI GTGCAG 3 cut(s) 15, 42, 48
BshFI GGCC 1 cut(s) 178
BsiHKAI GWGCWC 1 cut(s) 55
BsmAI GTCTC 1 cut(s) 211
BsmI GAATGC 1 cut(s) 275
BsnI GGCC 1 cut(s) 178
Bsp1286I GDGCHC 1 cut(s) 55
Bsp143I GATC 2 cut(s) 26, 125
BspACI CCGC 1 cut(s) 111
BspANI GGCC 1 cut(s) 178
BspCNI CTCAG 1 cut(s) 112
BspLI GGNNCC 1 cut(s) 81
BspMI ACCTGC 2 cut(s) 64, 70
BspOI GCTAGC 1 cut(s) 234
BspPI GGATC 1 cut(s) 133
BsrDI GCAATG 1 cut(s) 132
BsrI ACTGG 2 cut(s) 68, 322
BssECI CCNNGG 1 cut(s) 270
BssMI GATC 2 cut(s) 26, 125
BssT1I CCWWGG 1 cut(s) 270
Bst2UI CCWGG 1 cut(s) 77
Bst4CI ACNGT 2 cut(s) 17, 202
BstAPI GCANNNNNTGC 1 cut(s) 227
BstC8I GCNNGC 2 cut(s) 232, 256
BstDEI CTNAG 2 cut(s) 99, 374
BstH2I RGCGCY 1 cut(s) 159
BstHHI GCGC 1 cut(s) 158
BstKTI GATC 2 cut(s) 29, 128
BstMAI GTCTC 1 cut(s) 211
BstMBI GATC 2 cut(s) 26, 125
BstMWI GCNNNNNNNGC 4 cut(s) 38, 155, 218, 227
BstNI CCWGG 1 cut(s) 77
BstNSI RCATGY 1 cut(s) 373
BstSCI CCNGG 1 cut(s) 75
BstSFI CTRYAG 1 cut(s) 201
BstSLI GKGCMC 1 cut(s) 55
BstV1I GCAGC 1 cut(s) 199
BsuRI GGCC 1 cut(s) 178
BtrI CACGTC 1 cut(s) 35
BtsI GCAGTG 1 cut(s) 265
BtsIMutI CAGTG 3 cut(s) 22, 265, 382
BveI ACCTGC 2 cut(s) 64, 70
Cac8I GCNNGC 2 cut(s) 232, 256
CaiI CAGNNNCTG 1 cut(s) 95
CfoI GCGC 1 cut(s) 158
Cfr13I GGNCC 1 cut(s) 176
Csp6I GTAC 1 cut(s) 183
CviAII CATG 3 cut(s) 194, 331, 370
CviJI RGCY 5 cut(s) 41, 80, 92, 149, 178
CviKI_1 RGCY 5 cut(s) 41, 80, 92, 149, 178
CviQI GTAC 1 cut(s) 183
DdeI CTNAG 2 cut(s) 99, 374
DpnI GATC 2 cut(s) 28, 127
DpnII GATC 2 cut(s) 26, 125
Eco130I CCWWGG 1 cut(s) 270
Eco32I GATATC 1 cut(s) 310
Eco57I CTGAAG 1 cut(s) 159
EcoRII CCWGG 1 cut(s) 75
EcoRV GATATC 1 cut(s) 310
EcoT14I CCWWGG 1 cut(s) 270
ErhI CCWWGG 1 cut(s) 270
FaeI CATG 3 cut(s) 197, 334, 373
FaiI YATR 5 cut(s) 195, 239, 332, 338, 371
FatI CATG 3 cut(s) 193, 330, 369
Fnu4HI GCNGC 1 cut(s) 213
Fsp4HI GCNGC 1 cut(s) 213
FspBI CTAG 1 cut(s) 231
GlaI GCGC 1 cut(s) 157
GluI GCNGC 1 cut(s) 213
GsuI CTGGAG 2 cut(s) 72, 201
HaeII RGCGCY 1 cut(s) 159
HaeIII GGCC 1 cut(s) 178
HhaI GCGC 1 cut(s) 158
Hin1II CATG 3 cut(s) 197, 334, 373
Hin6I GCGC 1 cut(s) 156
HinP1I GCGC 1 cut(s) 156
HinfI GANTC 1 cut(s) 164
Hpy166II GTNNAC 2 cut(s) 53, 265
Hpy188I TCNGA 4 cut(s) 102, 250, 346, 367
Hpy8I GTNNAC 2 cut(s) 53, 265
Hpy99I CGWCG 1 cut(s) 51
HpyAV CCTTC 2 cut(s) 14, 134
HpyCH4III ACNGT 2 cut(s) 17, 202
HpyCH4IV ACGT 1 cut(s) 34
HpyCH4V TGCA 6 cut(s) 32, 53, 59, 65, 258, 351
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 155, 218, 227
HpyF3I CTNAG 2 cut(s) 99, 374
HpySE526I ACGT 1 cut(s) 34
Hsp92II CATG 3 cut(s) 197, 334, 373
HspAI GCGC 1 cut(s) 156
Kzo9I GATC 2 cut(s) 26, 125
LmnI GCTCC 5 cut(s) 46, 85, 91, 97, 220
Lsp1109I GCAGC 1 cut(s) 199
LweI GCATC 1 cut(s) 241
MaeI CTAG 1 cut(s) 231
MaeII ACGT 1 cut(s) 34
MalI GATC 2 cut(s) 28, 127
MboI GATC 2 cut(s) 26, 125
MboII GAAGA 1 cut(s) 367
MhlI GDGCHC 1 cut(s) 55
MnlI CCTC 2 cut(s) 54, 189
MslI CAYNNNNRTG 1 cut(s) 335
MspR9I CCNGG 1 cut(s) 77
Mva1269I GAATGC 1 cut(s) 275
MvaI CCWGG 1 cut(s) 77
MwoI GCNNNNNNNGC 4 cut(s) 38, 155, 218, 227
NdeII GATC 2 cut(s) 26, 125
NheI GCTAGC 1 cut(s) 230
NlaIII CATG 3 cut(s) 197, 334, 373
NlaIV GGNNCC 1 cut(s) 81
NspI RCATGY 1 cut(s) 373
PaqCI CACCTGC 2 cut(s) 64, 70
PcsI WCGNNNNNNNCGW 1 cut(s) 31
PctI GAATGC 1 cut(s) 275
PfeI GAWTC 1 cut(s) 164
PkrI GCNGC 1 cut(s) 214
Psp6I CCWGG 1 cut(s) 75
PspGI CCWGG 1 cut(s) 75
PspN4I GGNNCC 1 cut(s) 81
PspPI GGNCC 1 cut(s) 176
PsrI GAACNNNNNNTAC 2 cut(s) 229, 261
PstNI CAGNNNCTG 1 cut(s) 95
RsaI GTAC 1 cut(s) 184
RsaNI GTAC 1 cut(s) 183
RseI CAYNNNNRTG 1 cut(s) 335
SatI GCNGC 1 cut(s) 213
Sau3AI GATC 2 cut(s) 26, 125
Sau96I GGNCC 1 cut(s) 176
ScrFI CCNGG 1 cut(s) 77
SduI GDGCHC 1 cut(s) 55
SetI ASST 7 cut(s) 25, 37, 43, 58, 64, 94, 226
SfaNI GCATC 1 cut(s) 241
SfcI CTRYAG 1 cut(s) 201
SmiMI CAYNNNNRTG 1 cut(s) 335
SsiI CCGC 1 cut(s) 111
SspMI CTAG 1 cut(s) 231
StyD4I CCNGG 1 cut(s) 75
StyI CCWWGG 1 cut(s) 270
TaaI ACNGT 2 cut(s) 17, 202
TaiI ACGT 1 cut(s) 37
TaqI TCGA 1 cut(s) 25
TfiI GAWTC 1 cut(s) 164
TscAI CASTG 3 cut(s) 22, 265, 382
TseI GCWGC 1 cut(s) 212
TspDTI ATGAA 1 cut(s) 210
TspRI CASTG 3 cut(s) 22, 265, 382
VneI GTGCAC 1 cut(s) 51
XceI RCATGY 1 cut(s) 373
XspI CTAG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.