Rorug06G0054800

Hemiasterlin resistant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004697
Physical Location & Seq
Reverse (-)
7546212 .. 7548200
1989 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0054800.1

Sequence Viewer

Length: 1404 bp
ATGAATTTGAAATTCCAAGGCTTGGCGCTCCAGCTCTTGCTTGATTGGAACCTCCCATCTCAGCTCTCAGGACATTATTATGTTCTTGGTCTCCTGGACTCAAGCTTCATGTATCTTCTTTCAAACTCTCTGCTAGCTAGGCTCTATAAAGCTTGTTATTTTCCTTCCGGTGATTTTTGGTCAGCTAGGGTTTCAGGTTCTATGTCTGCTTGTTGGCGGGGCATAATGGCAGCTAAAGCTGTGTTGATGAGGGGTGTCAGATGGCAGGTGGGTGATGGTAATTCTATCCGGATTTGGTCTGACCCTTGGATACCTCGGCCTTCTTCTTTTCGTCCACTATTTCGACCCAATACTCCTGTGACTCGGGTTAGTGACTTAATTTCTGATTCTTGTTGGGATGCTGCACTTATTGATACTATTTTTGATCCTGCTGATGCGGATTTGATATCAGCTATTCCCTTGAGTTCAAGGTTAGTCCCAGATAGGCTTATTTGGCATTTTGATGCTAAAGGGCTTTTTACCACTAAAACTGCTTATGTTCAAGCCTTTACCTGGCTGCATTCTCCTGCTTCTGCTTCTAATGATGATTCTGTTACCTTGCTTTGGAAACGGTTATGGGCAGCTAAAGTTCCGGGTAAGGTTAAAGTGCATACTTGGAGATTTTGTTCTTCTATTCTACCCACTATTTCTCAGCTGTGTAAAAAGCATGTTTATATTGAGGGTGGCTGCGTTTTATGCAACAATGCGGATGAGTCTGTTGAGCATGTTAGTCGAGATTGTCCCTTTGTTAAGGGTATTCTCTACCGGTGCCCGGGGTTGGGAGGTATTTTTGCTAGCCATTCTGAGGCCTCTTGCTTGGATTGGTTGCAGGTATGTTCTGATTCTCTTTCTGAAGATCTCTTTTCTCTTCTCTTATGGGGAATTTGGTCTATTTGGAAAGAAAGGAATAAGAGGGTTTGGCAGGATAAATGGTCTTCTCCTGAGCAAGTGAGGTATGGTATTTATTCTCAACAGCTACTTTTCAATTCAATTGGACTGCGTCAGAGGCGGGTTGGGAGTAGGGTAATTAAACCTTGGTCTCCTCCTCCAGTTGGGTGGTTGAAAGCTAACATTGCTGGTGCTTTTGATAAGAATTCATGTTCTGGTGGTATTGGGGTGGTTGTGCGGGACTCCGATGGGTTGATTGTAGGTGGTTGTTGCTGCAAAGTTAATAGGGTTTTGTCTCCGGCACTGGTGGAGGCTCTAGCTGCTCGAAAGGCTTGTCACCTTGCTGTGCAATATTATGAGCGGTACAAGGATCGCCCTTGTGGCTTCTTGAGTATGACCCCGGTTTACACGTTCAGAGATGATGCTACGATGGAAGCACCAAATGATGAAATGCGCAATCTTAATCTGAAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CHCH

Protein Analysis

467

Amino Acids

52.43

Weight (kDa)

8.95

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 197 - 264 1.1e-13 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 256
Acc16I TGCGCA 1 cut(s) 1382
Acc36I ACCTGC 2 cut(s) 256, 859
AccB1I GGYRCC 1 cut(s) 807
AccB7I CCANNNNNTGG 1 cut(s) 22
AccBSI CCGCTC 1 cut(s) 1288
AccIII TCCGGA 1 cut(s) 288
AciI CCGC 6 cut(s) 217, 437, 746, 1048, 1165, 1288
AclWI GGATC 2 cut(s) 419, 1305
AcsI RAATTY 4 cut(s) 4, 11, 921, 1132
AcuI CTGAAG 1 cut(s) 912
AfaI GTAC 1 cut(s) 1292
AfiI CCNNNNNNNGG 8 cut(s) 22, 552, 603, 811, 817, 818, 844, 1091
AflIII ACRYGT 1 cut(s) 1335
AgeI ACCGGT 1 cut(s) 804
AgsI TTSAA 7 cut(s) 10, 123, 468, 542, 1024, 1029, 1102
AjnI CCWGG 2 cut(s) 93, 551
AloI GAACNNNNNNTCC 2 cut(s) 649, 681
Alw26I GTCTC 3 cut(s) 95, 1083, 1227
AlwI GGATC 2 cut(s) 419, 1305
Ama87I CYCGRG 2 cut(s) 363, 811
Aor13HI TCCGGA 1 cut(s) 288
AoxI GGCC 2 cut(s) 317, 846
ApeKI GCWGC 7 cut(s) 230, 401, 556, 620, 726, 1200, 1247
ApoI RAATTY 4 cut(s) 4, 11, 921, 1132
ArsI GACNNNNNNTTYG 2 cut(s) 1246, 1278
AsiGI ACCGGT 1 cut(s) 804
AspLEI GCGC 2 cut(s) 28, 1383
AsuC2I CCSGG 4 cut(s) 633, 812, 813, 1328
AsuHPI GGTGA 3 cut(s) 182, 284, 1256
AsuNHI GCTAGC 2 cut(s) 133, 833
AvaI CYCGRG 2 cut(s) 363, 811
BaeGI GKGCMC 1 cut(s) 812
BanI GGYRCC 1 cut(s) 807
BbsI GAAGAC 1 cut(s) 966
BbvI GCAGC 7 cut(s) 242, 388, 543, 632, 713, 1187, 1234
BccI CCATC 5 cut(s) 64, 255, 269, 1169, 1351
BcgI CGANNNNNNTGC 2 cut(s) 752, 786
BciT130I CCWGG 2 cut(s) 95, 553
BciVI GTATCC 1 cut(s) 303
BcnI CCSGG 4 cut(s) 633, 812, 813, 1328
BcoDI GTCTC 3 cut(s) 95, 1083, 1227
BfaI CTAG 5 cut(s) 134, 138, 186, 834, 1244
BfoI RGCGCY 1 cut(s) 29
BfuAI ACCTGC 2 cut(s) 256, 859
BfuI GTATCC 1 cut(s) 303
BglI GCCNNNNNGGC 1 cut(s) 1308
BglII AGATCT 1 cut(s) 895
BisI GCNGC 7 cut(s) 231, 402, 557, 621, 727, 1201, 1248
BlsI GCNGC 7 cut(s) 232, 403, 558, 622, 728, 1202, 1249
Bme1390I CCNGG 6 cut(s) 95, 553, 633, 812, 813, 1328
BmeT110I CYCGRG 2 cut(s) 363, 811
BmiI GGNNCC 2 cut(s) 50, 809
BmrFI CCNGG 6 cut(s) 95, 553, 633, 812, 813, 1328
BmsI GCATC 4 cut(s) 388, 424, 493, 1339
BmtI GCTAGC 2 cut(s) 137, 837
BpiI GAAGAC 1 cut(s) 966
BpmI CTGGAG 2 cut(s) 14, 1071
Bpu10I CCTNAGC 1 cut(s) 981
BpuEI CTTGAG 3 cut(s) 85, 481, 1336
BpuMI CCSGG 4 cut(s) 633, 812, 813, 1328
BsaI GGTCTC 2 cut(s) 95, 1083
BsaJI CCNNGG 7 cut(s) 16, 305, 314, 811, 812, 1073, 1326
BsaWI WCCGGW 3 cut(s) 167, 288, 804
BsaXI ACNNNNNCTCC 2 cut(s) 649, 679
Bsc4I CCNNNNNNNGG 8 cut(s) 22, 552, 603, 811, 817, 818, 844, 1091
Bse118I RCCGGY 1 cut(s) 804
Bse1I ACTGG 2 cut(s) 1088, 1236
Bse3DI GCAATG 1 cut(s) 1110
BseAI TCCGGA 1 cut(s) 288
BseBI CCWGG 2 cut(s) 95, 553
BseDI CCNNGG 7 cut(s) 16, 305, 314, 811, 812, 1073, 1326
BseGI GGATG 2 cut(s) 403, 754
BseLI CCNNNNNNNGG 8 cut(s) 22, 552, 603, 811, 817, 818, 844, 1091
BseMI GCAATG 1 cut(s) 1110
BseMII CTCAG 5 cut(s) 74, 81, 704, 834, 972
BseNI ACTGG 2 cut(s) 1088, 1236
BseRI GAGGAG 2 cut(s) 1071, 1074
BseSI GKGCMC 1 cut(s) 812
BseXI GCAGC 7 cut(s) 242, 388, 543, 632, 713, 1187, 1234
BsgI GTGCAG 1 cut(s) 387
BshFI GGCC 2 cut(s) 319, 848
BshNI GGYRCC 1 cut(s) 807
BshTI ACCGGT 1 cut(s) 804
BsiHKCI CYCGRG 2 cut(s) 363, 811
BsiSI CCGG 7 cut(s) 168, 289, 632, 805, 812, 1226, 1328
BslFI GGGAC 3 cut(s) 461, 765, 1181
BslI CCNNNNNNNGG 8 cut(s) 22, 552, 603, 811, 817, 818, 844, 1091
BsmAI GTCTC 3 cut(s) 95, 1083, 1227
BsmFI GGGAC 3 cut(s) 461, 765, 1181
BsmI GAATGC 1 cut(s) 559
BsnI GGCC 2 cut(s) 319, 848
Bso31I GGTCTC 2 cut(s) 95, 1083
BsoBI CYCGRG 2 cut(s) 363, 811
Bsp1286I GDGCHC 1 cut(s) 812
Bsp13I TCCGGA 1 cut(s) 288
Bsp143I GATC 3 cut(s) 424, 895, 1297
BspACI CCGC 6 cut(s) 217, 437, 746, 1048, 1165, 1288
BspANI GGCC 2 cut(s) 319, 848
BspCNI CTCAG 5 cut(s) 73, 80, 703, 835, 973
BspEI TCCGGA 1 cut(s) 288
BspLI GGNNCC 2 cut(s) 50, 809
BspMI ACCTGC 2 cut(s) 256, 859
BspOI GCTAGC 2 cut(s) 137, 837
BspPI GGATC 2 cut(s) 419, 1305
BspT107I GGYRCC 1 cut(s) 807
BspTNI GGTCTC 2 cut(s) 95, 1083
BsrBI CCGCTC 1 cut(s) 1288
BsrDI GCAATG 1 cut(s) 1110
BsrFI RCCGGY 1 cut(s) 804
BsrI ACTGG 2 cut(s) 1088, 1236
BssAI RCCGGY 1 cut(s) 804
BssECI CCNNGG 7 cut(s) 16, 305, 314, 811, 812, 1073, 1326
BssMI GATC 3 cut(s) 424, 895, 1297
BssT1I CCWWGG 3 cut(s) 16, 305, 1073
Bst2UI CCWGG 2 cut(s) 95, 553
Bst4CI ACNGT 1 cut(s) 612
Bst6I CTCTTC 2 cut(s) 912, 1391
BstC8I GCNNGC 2 cut(s) 135, 835
BstDEI CTNAG 5 cut(s) 60, 67, 690, 843, 981
BstF5I GGATG 2 cut(s) 403, 754
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 2 cut(s) 28, 1383
BstKTI GATC 3 cut(s) 427, 898, 1300
BstMAI GTCTC 3 cut(s) 95, 1083, 1227
BstMBI GATC 3 cut(s) 424, 895, 1297
BstMWI GCNNNNNNNGC 9 cut(s) 139, 236, 493, 735, 1045, 1112, 1247, 1256, 1308
BstNI CCWGG 2 cut(s) 95, 553
BstNSI RCATGY 2 cut(s) 710, 767
BstSCI CCNGG 6 cut(s) 93, 551, 631, 810, 811, 1326
BstSLI GKGCMC 1 cut(s) 812
BstV1I GCAGC 7 cut(s) 242, 388, 543, 632, 713, 1187, 1234
BstV2I GAAGAC 1 cut(s) 966
BstX2I RGATCY 1 cut(s) 895
BstXI CCANNNNNNTGG 1 cut(s) 1095
BstYI RGATCY 1 cut(s) 895
BsuI GTATCC 1 cut(s) 303
BsuRI GGCC 2 cut(s) 319, 848
BtsCI GGATG 2 cut(s) 403, 754
BtsIMutI CAGTG 1 cut(s) 1229
BveI ACCTGC 2 cut(s) 256, 859
Cac8I GCNNGC 2 cut(s) 135, 835
CfoI GCGC 2 cut(s) 28, 1383
Cfr10I RCCGGY 1 cut(s) 804
Cfr9I CCCGGG 1 cut(s) 811
CseI GACGC 1 cut(s) 1028
Csp6I GTAC 1 cut(s) 1291
CspAI ACCGGT 1 cut(s) 804
CviAII CATG 4 cut(s) 109, 707, 764, 1137
CviQI GTAC 1 cut(s) 1291
DdeI CTNAG 5 cut(s) 60, 67, 690, 843, 981
DpnI GATC 3 cut(s) 426, 897, 1299
DpnII GATC 3 cut(s) 424, 895, 1297
Eam1104I CTCTTC 2 cut(s) 912, 1391
EarI CTCTTC 2 cut(s) 912, 1391
Eco130I CCWWGG 3 cut(s) 16, 305, 1073
Eco147I AGGCCT 1 cut(s) 848
Eco31I GGTCTC 2 cut(s) 95, 1083
Eco32I GATATC 1 cut(s) 447
Eco57I CTGAAG 1 cut(s) 912
Eco88I CYCGRG 2 cut(s) 363, 811
EcoRI GAATTC 1 cut(s) 1132
EcoRII CCWGG 2 cut(s) 93, 551
EcoRV GATATC 1 cut(s) 447
EcoT14I CCWWGG 3 cut(s) 16, 305, 1073
ErhI CCWWGG 3 cut(s) 16, 305, 1073
FaeI CATG 4 cut(s) 112, 710, 767, 1140
FaqI GGGAC 3 cut(s) 461, 765, 1181
FatI CATG 4 cut(s) 108, 706, 763, 1136
FauI CCCGC 3 cut(s) 210, 1041, 1158
Fnu4HI GCNGC 7 cut(s) 231, 402, 557, 621, 727, 1201, 1248
FokI GGATG 2 cut(s) 410, 761
Fsp4HI GCNGC 7 cut(s) 231, 402, 557, 621, 727, 1201, 1248
FspBI CTAG 5 cut(s) 134, 138, 186, 834, 1244
FspI TGCGCA 1 cut(s) 1382
GlaI GCGC 2 cut(s) 27, 1382
GluI GCNGC 7 cut(s) 231, 402, 557, 621, 727, 1201, 1248
GsuI CTGGAG 2 cut(s) 14, 1071
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 2 cut(s) 319, 848
HapII CCGG 7 cut(s) 168, 289, 632, 805, 812, 1226, 1328
HgaI GACGC 1 cut(s) 1028
HhaI GCGC 2 cut(s) 28, 1383
Hin1II CATG 4 cut(s) 112, 710, 767, 1140
Hin6I GCGC 2 cut(s) 26, 1381
HinP1I GCGC 2 cut(s) 26, 1381
HindIII AAGCTT 2 cut(s) 103, 150
HinfI GANTC 7 cut(s) 98, 361, 386, 587, 752, 881, 1169
HpaII CCGG 7 cut(s) 168, 289, 632, 805, 812, 1226, 1328
HphI GGTGA 3 cut(s) 182, 284, 1256
Hpy166II GTNNAC 2 cut(s) 335, 1333
Hpy188III TCNNGA 5 cut(s) 69, 289, 773, 980, 1315
Hpy8I GTNNAC 2 cut(s) 335, 1333
HpyAV CCTTC 2 cut(s) 174, 330
HpyCH4III ACNGT 1 cut(s) 612
HpyCH4IV ACGT 1 cut(s) 1337
HpyCH4V TGCA 7 cut(s) 404, 559, 649, 738, 868, 1203, 1276
HpyF10VI GCNNNNNNNGC 9 cut(s) 139, 236, 493, 735, 1045, 1112, 1247, 1256, 1308
HpyF3I CTNAG 5 cut(s) 60, 67, 690, 843, 981
HpySE526I ACGT 1 cut(s) 1337
Hsp92II CATG 4 cut(s) 112, 710, 767, 1140
HspAI GCGC 2 cut(s) 26, 1381
Kpn2I TCCGGA 1 cut(s) 288
Kzo9I GATC 3 cut(s) 424, 895, 1297
LmnI GCTCC 1 cut(s) 33
Lsp1109I GCAGC 7 cut(s) 242, 388, 543, 632, 713, 1187, 1234
LweI GCATC 4 cut(s) 388, 424, 493, 1339
MaeI CTAG 5 cut(s) 134, 138, 186, 834, 1244
MaeII ACGT 1 cut(s) 1337
MaeIII GTNAC 4 cut(s) 358, 371, 592, 1262
MalI GATC 3 cut(s) 426, 897, 1299
MbiI CCGCTC 1 cut(s) 1288
MboI GATC 3 cut(s) 424, 895, 1297
MboII GAAGA 6 cut(s) 107, 315, 660, 899, 905, 966
MfeI CAATTG 1 cut(s) 1029
MflI RGATCY 1 cut(s) 895
MhlI GDGCHC 1 cut(s) 812
MluCI AATT 9 cut(s) 4, 11, 280, 378, 921, 1024, 1029, 1065, 1132
MlyI GAGTC 4 cut(s) 92, 355, 761, 1163
MroI TCCGGA 1 cut(s) 288
MseI TTAA 6 cut(s) 377, 642, 789, 1068, 1209, 1389
MslI CAYNNNNRTG 2 cut(s) 78, 501
MspA1I CMGCKG 1 cut(s) 694
MspI CCGG 7 cut(s) 168, 289, 632, 805, 812, 1226, 1328
MspR9I CCNGG 6 cut(s) 95, 553, 633, 812, 813, 1328
MunI CAATTG 1 cut(s) 1029
Mva1269I GAATGC 1 cut(s) 559
MvaI CCWGG 2 cut(s) 95, 553
MwoI GCNNNNNNNGC 9 cut(s) 139, 236, 493, 735, 1045, 1112, 1247, 1256, 1308
NciI CCSGG 4 cut(s) 633, 812, 813, 1328
NdeII GATC 3 cut(s) 424, 895, 1297
NheI GCTAGC 2 cut(s) 133, 833
NlaIII CATG 4 cut(s) 112, 710, 767, 1140
NlaIV GGNNCC 2 cut(s) 50, 809
NmeAIII GCCGAG 1 cut(s) 295
NmuCI GTSAC 3 cut(s) 358, 371, 1262
NsbI TGCGCA 1 cut(s) 1382
NspI RCATGY 2 cut(s) 710, 767
PaqCI CACCTGC 1 cut(s) 256
PceI AGGCCT 1 cut(s) 848
PctI GAATGC 1 cut(s) 559
PfeI GAWTC 3 cut(s) 386, 587, 881
PflFI GACNNNGTC 1 cut(s) 1038
PflMI CCANNNNNTGG 1 cut(s) 22
PfoI TCCNGGA 1 cut(s) 93
PinAI ACCGGT 1 cut(s) 804
PkrI GCNGC 7 cut(s) 232, 403, 558, 622, 728, 1202, 1249
PleI GAGTC 4 cut(s) 92, 355, 760, 1163
PpsI GAGTC 4 cut(s) 92, 355, 760, 1163
Psp6I CCWGG 2 cut(s) 93, 551
PspGI CCWGG 2 cut(s) 93, 551
PspN4I GGNNCC 2 cut(s) 50, 809
PsuI RGATCY 1 cut(s) 895
PsyI GACNNNGTC 1 cut(s) 1038
PvuII CAGCTG 1 cut(s) 694
RsaI GTAC 1 cut(s) 1292
RsaNI GTAC 1 cut(s) 1291
RseI CAYNNNNRTG 2 cut(s) 78, 501
SaqAI TTAA 6 cut(s) 377, 642, 789, 1068, 1209, 1389
SatI GCNGC 7 cut(s) 231, 402, 557, 621, 727, 1201, 1248
Sau3AI GATC 3 cut(s) 424, 895, 1297
SchI GAGTC 4 cut(s) 92, 355, 761, 1163
ScrFI CCNGG 6 cut(s) 95, 553, 633, 812, 813, 1328
SduI GDGCHC 1 cut(s) 812
SfaNI GCATC 4 cut(s) 388, 424, 493, 1339
SmaI CCCGGG 1 cut(s) 813
SmiMI CAYNNNNRTG 2 cut(s) 78, 501
SmlI CTYRAG 3 cut(s) 100, 460, 1315
SmoI CTYRAG 3 cut(s) 100, 460, 1315
Sse9I AATT 9 cut(s) 4, 11, 280, 378, 921, 1024, 1029, 1065, 1132
SseBI AGGCCT 1 cut(s) 848
SsiI CCGC 6 cut(s) 217, 437, 746, 1048, 1165, 1288
SspI AATATT 1 cut(s) 1280
SspMI CTAG 5 cut(s) 134, 138, 186, 834, 1244
StuI AGGCCT 1 cut(s) 848
StyD4I CCNGG 6 cut(s) 93, 551, 631, 810, 811, 1326
StyI CCWWGG 3 cut(s) 16, 305, 1073
TaaI ACNGT 1 cut(s) 612
TaiI ACGT 1 cut(s) 1340
TaqI TCGA 3 cut(s) 343, 772, 1252
TasI AATT 9 cut(s) 4, 11, 280, 378, 921, 1024, 1029, 1065, 1132
TfiI GAWTC 3 cut(s) 386, 587, 881
Tru1I TTAA 6 cut(s) 377, 642, 789, 1068, 1209, 1389
Tru9I TTAA 6 cut(s) 377, 642, 789, 1068, 1209, 1389
TscAI CASTG 1 cut(s) 1236
TseFI GTSAC 3 cut(s) 358, 371, 1262
TseI GCWGC 7 cut(s) 230, 401, 556, 620, 726, 1200, 1247
Tsp45I GTSAC 3 cut(s) 358, 371, 1262
TspDTI ATGAA 4 cut(s) 17, 97, 1125, 1389
TspMI CCCGGG 1 cut(s) 811
TspRI CASTG 1 cut(s) 1236
Tth111I GACNNNGTC 1 cut(s) 1038
Van91I CCANNNNNTGG 1 cut(s) 22
XapI RAATTY 4 cut(s) 4, 11, 921, 1132
XceI RCATGY 2 cut(s) 710, 767
XmaI CCCGGG 1 cut(s) 811
XspI CTAG 5 cut(s) 134, 138, 186, 834, 1244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.