MD05G1048900.v1.1

CHCH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004697
Physical Location & Seq
Reverse (-)
8349889 .. 8352239
2351 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1048900.v1.1.491

Sequence Viewer

Length: 435 bp
ATGGCTCGCCGCTCAGGAGGTCGATCTGCCCGCCCGGCTCCCCGTCATGCACCTGCACGCAACCCTCCTCAGCCTGTTCGCCAAGCTCCTCCTCCAGCTCCAGCTCAGTCTGGAAGTGGTGGATCTATGCTTTCCGGAATTGGATCAACCATAGCACAGGGTATGGCTTTTGGTACTGGAAGTGCTGTGGCACACAGAGCTGTGGATGCTGTGATGGGTCCTCGCACTATTCAACATGAAACTGTAGTCTCTGAGGCCACTGCTGGTGCTCCAGTCCCCATGGCTAGCAGCATGGGTGGCTCAGATGCATGCAGTAATCAGTCCAAGGCATTCCAAGATTGTGTCAACCACTTTGGAAGTGATATAAGCAAGTGCCAGTTCTACATGGATATGCTGTCCGAGTGCAAGAAGAACTCCGGCATGTTGAATGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CHCH

Protein Analysis

145

Amino Acids

14.71

Weight (kDa)

9.43

Isoelectric Point (pI)

67.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHCH PF06747 104 - 138 9e-08 CHCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 61
Acc36I ACCTGC 1 cut(s) 61
AccBSI CCGCTC 1 cut(s) 12
AccIII TCCGGA 1 cut(s) 134
AciI CCGC 2 cut(s) 10, 31
AclWI GGATC 2 cut(s) 130, 151
AfaI GTAC 1 cut(s) 175
AgsI TTSAA 2 cut(s) 233, 427
AluBI AGCT 4 cut(s) 86, 98, 104, 200
AluI AGCT 4 cut(s) 86, 98, 104, 200
Alw21I GWGCWC 1 cut(s) 271
Alw26I GTCTC 1 cut(s) 253
AlwI GGATC 2 cut(s) 130, 151
Aor13HI TCCGGA 1 cut(s) 134
AoxI GGCC 1 cut(s) 255
ApeKI GCWGC 1 cut(s) 288
AspS9I GGNCC 1 cut(s) 218
AsuC2I CCSGG 1 cut(s) 35
AsuNHI GCTAGC 1 cut(s) 284
AvaII GGWCC 1 cut(s) 218
Bbv12I GWGCWC 1 cut(s) 271
BbvCI CCTCAGC 1 cut(s) 69
BbvI GCAGC 1 cut(s) 300
BccI CCATC 1 cut(s) 208
BcnI CCSGG 1 cut(s) 35
BcoDI GTCTC 1 cut(s) 253
BfaI CTAG 1 cut(s) 285
BfmI CTRYAG 1 cut(s) 243
BfuAI ACCTGC 1 cut(s) 61
BglI GCCNNNNNGGC 1 cut(s) 35
BisI GCNGC 2 cut(s) 10, 289
BlsI GCNGC 2 cut(s) 11, 290
Bme1390I CCNGG 1 cut(s) 35
Bme18I GGWCC 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 218
BmiI GGNNCC 2 cut(s) 39, 219
BmrFI CCNGG 1 cut(s) 35
BmsI GCATC 2 cut(s) 196, 295
BmtI GCTAGC 1 cut(s) 288
BpmI CTGGAG 3 cut(s) 78, 84, 255
Bpu10I CCTNAGC 2 cut(s) 13, 69
BpuMI CCSGG 1 cut(s) 35
BsaJI CCNNGG 2 cut(s) 279, 324
BsaWI WCCGGW 1 cut(s) 134
Bse1I ACTGG 3 cut(s) 181, 272, 376
BseAI TCCGGA 1 cut(s) 134
BseDI CCNNGG 2 cut(s) 279, 324
BseGI GGATG 1 cut(s) 211
BseMII CTCAG 5 cut(s) 27, 83, 119, 243, 315
BseNI ACTGG 3 cut(s) 181, 272, 376
BseRI GAGGAG 3 cut(s) 57, 78, 81
BseXI GCAGC 1 cut(s) 300
BsgI GTGCAG 1 cut(s) 39
BshFI GGCC 1 cut(s) 257
BsiHKAI GWGCWC 1 cut(s) 271
BsiSI CCGG 3 cut(s) 35, 135, 417
BslFI GGGAC 1 cut(s) 260
BsmAI GTCTC 1 cut(s) 253
BsmFI GGGAC 1 cut(s) 260
BsmI GAATGC 2 cut(s) 329, 433
BsnI GGCC 1 cut(s) 257
Bsp1286I GDGCHC 1 cut(s) 271
Bsp13I TCCGGA 1 cut(s) 134
Bsp143I GATC 3 cut(s) 23, 122, 143
Bsp19I CCATGG 1 cut(s) 279
BspACI CCGC 2 cut(s) 10, 31
BspANI GGCC 1 cut(s) 257
BspCNI CTCAG 5 cut(s) 26, 82, 118, 244, 314
BspEI TCCGGA 1 cut(s) 134
BspLI GGNNCC 2 cut(s) 39, 219
BspMI ACCTGC 1 cut(s) 61
BspOI GCTAGC 1 cut(s) 288
BspPI GGATC 2 cut(s) 130, 151
BsrBI CCGCTC 1 cut(s) 12
BsrI ACTGG 3 cut(s) 181, 272, 376
BssECI CCNNGG 2 cut(s) 279, 324
BssMI GATC 3 cut(s) 23, 122, 143
BssT1I CCWWGG 2 cut(s) 279, 324
Bst4CI ACNGT 1 cut(s) 244
BstC8I GCNNGC 5 cut(s) 7, 31, 58, 286, 310
BstDEI CTNAG 5 cut(s) 13, 69, 105, 252, 301
BstDSI CCRYGG 1 cut(s) 279
BstF5I GGATG 1 cut(s) 211
BstKTI GATC 3 cut(s) 26, 125, 146
BstMAI GTCTC 1 cut(s) 253
BstMBI GATC 3 cut(s) 23, 122, 143
BstMWI GCNNNNNNNGC 4 cut(s) 35, 197, 206, 297
BstNSI RCATGY 2 cut(s) 312, 424
BstSCI CCNGG 1 cut(s) 33
BstSFI CTRYAG 1 cut(s) 243
BstV1I GCAGC 1 cut(s) 300
BstX2I RGATCY 1 cut(s) 122
BstYI RGATCY 1 cut(s) 122
BsuRI GGCC 1 cut(s) 257
BtgI CCRYGG 1 cut(s) 279
BtsCI GGATG 1 cut(s) 211
BtsI GCAGTG 1 cut(s) 258
BtsIMutI CAGTG 1 cut(s) 258
BveI ACCTGC 1 cut(s) 61
Cac8I GCNNGC 5 cut(s) 7, 31, 58, 286, 310
Cfr13I GGNCC 1 cut(s) 218
Csp6I GTAC 1 cut(s) 174
CviAII CATG 7 cut(s) 47, 236, 280, 292, 309, 385, 421
CviQI GTAC 1 cut(s) 174
DdeI CTNAG 5 cut(s) 13, 69, 105, 252, 301
DpnI GATC 3 cut(s) 25, 124, 145
DpnII GATC 3 cut(s) 23, 122, 143
Eco130I CCWWGG 2 cut(s) 279, 324
Eco47I GGWCC 1 cut(s) 218
EcoO109I RGGNCCY 1 cut(s) 218
EcoT14I CCWWGG 2 cut(s) 279, 324
EcoT22I ATGCAT 1 cut(s) 310
ErhI CCWWGG 2 cut(s) 279, 324
FaeI CATG 7 cut(s) 50, 239, 283, 295, 312, 388, 424
FaqI GGGAC 1 cut(s) 260
FatI CATG 7 cut(s) 46, 235, 279, 291, 308, 384, 420
FauI CCCGC 1 cut(s) 38
Fnu4HI GCNGC 2 cut(s) 10, 289
FokI GGATG 1 cut(s) 218
Fsp4HI GCNGC 2 cut(s) 10, 289
FspBI CTAG 1 cut(s) 285
GluI GCNGC 2 cut(s) 10, 289
GsuI CTGGAG 3 cut(s) 78, 84, 255
HaeIII GGCC 1 cut(s) 257
HapII CCGG 3 cut(s) 35, 135, 417
Hin1II CATG 7 cut(s) 50, 239, 283, 295, 312, 388, 424
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HpaII CCGG 3 cut(s) 35, 135, 417
Hpy166II GTNNAC 1 cut(s) 346
Hpy188I TCNGA 3 cut(s) 253, 304, 400
Hpy188III TCNNGA 3 cut(s) 15, 111, 135
Hpy8I GTNNAC 1 cut(s) 346
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4V TGCA 5 cut(s) 50, 56, 308, 312, 405
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 197, 206, 297
HpyF3I CTNAG 5 cut(s) 13, 69, 105, 252, 301
Hsp92II CATG 7 cut(s) 50, 239, 283, 295, 312, 388, 424
Kpn2I TCCGGA 1 cut(s) 134
Kzo9I GATC 3 cut(s) 23, 122, 143
LmnI GCTCC 4 cut(s) 43, 91, 103, 274
Lsp1109I GCAGC 1 cut(s) 300
LweI GCATC 2 cut(s) 196, 295
MaeI CTAG 1 cut(s) 285
MalI GATC 3 cut(s) 25, 124, 145
MbiI CCGCTC 1 cut(s) 12
MboI GATC 3 cut(s) 23, 122, 143
MboII GAAGA 1 cut(s) 421
MflI RGATCY 1 cut(s) 122
MhlI GDGCHC 1 cut(s) 271
MluCI AATT 1 cut(s) 138
MnlI CCTC 7 cut(s) 11, 75, 78, 99, 102, 231, 247
Mph1103I ATGCAT 1 cut(s) 310
MroI TCCGGA 1 cut(s) 134
MslI CAYNNNNRTG 1 cut(s) 389
MspI CCGG 3 cut(s) 35, 135, 417
MspR9I CCNGG 1 cut(s) 35
Mva1269I GAATGC 2 cut(s) 329, 433
MwoI GCNNNNNNNGC 4 cut(s) 35, 197, 206, 297
NciI CCSGG 1 cut(s) 35
NcoI CCATGG 1 cut(s) 279
NdeII GATC 3 cut(s) 23, 122, 143
NheI GCTAGC 1 cut(s) 284
NlaIII CATG 7 cut(s) 50, 239, 283, 295, 312, 388, 424
NlaIV GGNNCC 2 cut(s) 39, 219
NsiI ATGCAT 1 cut(s) 310
NspI RCATGY 2 cut(s) 312, 424
PaeI GCATGC 1 cut(s) 312
PaqCI CACCTGC 1 cut(s) 61
PctI GAATGC 2 cut(s) 329, 433
PkrI GCNGC 2 cut(s) 11, 290
PpuMI RGGWCCY 1 cut(s) 218
Psp5II RGGWCCY 1 cut(s) 218
PspN4I GGNNCC 2 cut(s) 39, 219
PspPI GGNCC 1 cut(s) 218
PspPPI RGGWCCY 1 cut(s) 218
PsuI RGATCY 1 cut(s) 122
RsaI GTAC 1 cut(s) 175
RsaNI GTAC 1 cut(s) 174
RseI CAYNNNNRTG 1 cut(s) 389
SatI GCNGC 2 cut(s) 10, 289
Sau3AI GATC 3 cut(s) 23, 122, 143
Sau96I GGNCC 1 cut(s) 218
ScrFI CCNGG 1 cut(s) 35
SduI GDGCHC 1 cut(s) 271
SetI ASST 6 cut(s) 22, 55, 88, 100, 106, 202
SfaNI GCATC 2 cut(s) 196, 295
SfcI CTRYAG 1 cut(s) 243
SinI GGWCC 1 cut(s) 218
SmiMI CAYNNNNRTG 1 cut(s) 389
SphI GCATGC 1 cut(s) 312
Sse9I AATT 1 cut(s) 138
SsiI CCGC 2 cut(s) 10, 31
SspMI CTAG 1 cut(s) 285
StyD4I CCNGG 1 cut(s) 33
StyI CCWWGG 2 cut(s) 279, 324
TaaI ACNGT 1 cut(s) 244
TaqI TCGA 1 cut(s) 22
TasI AATT 1 cut(s) 138
TauI GCSGC 1 cut(s) 12
TscAI CASTG 1 cut(s) 265
TseI GCWGC 1 cut(s) 288
TspDTI ATGAA 1 cut(s) 252
TspRI CASTG 1 cut(s) 265
VpaK11BI GGWCC 1 cut(s) 218
XceI RCATGY 2 cut(s) 312, 424
XspI CTAG 1 cut(s) 285
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.