pycom10g04270

CHCH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004697
Physical Location & Seq
Reverse (-)
4504411 .. 4506243
1833 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g04270.3

Sequence Viewer

Length: 438 bp
ATGGCTCGCCGAAGCTCAGGAGGTCGATCTGCTCGCCCAGCTCCTCGTCATGCACCTGCACGAAACCCGCCTCAGCCTGTTCGCCAAGCTCCTCCTCCAGCTGCAGCTCAGTCTGGAAGTGGTGGATCTATGCTTTCGGGAATTGGATCAACCATAGCCCAGGGTATGGCTTTTGGTACTGGAAGTGCCGTGGCACACAGGGCTGTGGATGCTGTGATGGGTCCTCGCACAATTCAACATGAAGCTGTAGTCTCTGAGGCTACTGCTGCTGCTCCAGTCCCCATGGTTAGCAGCATGGGTGGCTCAGATGCATGCAGTAATCACTCTAAGGCATTCCAAGATTGCGTCAACCACTTTGGAAGTGATATCAGCAAGTGCCAGTTCTACATGGATATGCTATCCGAGTGCAAGAGGAACTCCGGCATGTTGAATGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CHCH

Protein Analysis

146

Amino Acids

14.82

Weight (kDa)

9.49

Isoelectric Point (pI)

65.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 64
Acc36I ACCTGC 1 cut(s) 64
AccB7I CCANNNNNTGG 1 cut(s) 166
AciI CCGC 1 cut(s) 68
AclWI GGATC 2 cut(s) 133, 154
AfaI GTAC 1 cut(s) 178
AfiI CCNNNNNNNGG 1 cut(s) 166
AgsI TTSAA 2 cut(s) 236, 430
AjnI CCWGG 1 cut(s) 159
AluBI AGCT 6 cut(s) 15, 41, 89, 101, 107, 245
AluI AGCT 6 cut(s) 15, 41, 89, 101, 107, 245
Alw26I GTCTC 1 cut(s) 256
AlwI GGATC 2 cut(s) 133, 154
ApeKI GCWGC 5 cut(s) 101, 104, 266, 269, 291
AspS9I GGNCC 1 cut(s) 221
AvaII GGWCC 1 cut(s) 221
BbvCI CCTCAGC 1 cut(s) 72
BbvI GCAGC 5 cut(s) 88, 116, 253, 256, 303
BccI CCATC 1 cut(s) 211
BceAI ACGGC 1 cut(s) 173
BciT130I CCWGG 1 cut(s) 161
BcoDI GTCTC 1 cut(s) 256
BfmI CTRYAG 2 cut(s) 102, 246
BfuAI ACCTGC 1 cut(s) 64
BisI GCNGC 5 cut(s) 102, 105, 267, 270, 292
BlsI GCNGC 5 cut(s) 103, 106, 268, 271, 293
Bme1390I CCNGG 1 cut(s) 161
Bme18I GGWCC 1 cut(s) 221
BmgT120I GGNCC 1 cut(s) 221
BmiI GGNNCC 1 cut(s) 222
BmrFI CCNGG 1 cut(s) 161
BmsI GCATC 2 cut(s) 199, 298
BpmI CTGGAG 2 cut(s) 81, 258
Bpu10I CCTNAGC 2 cut(s) 16, 72
BsaJI CCNNGG 4 cut(s) 159, 160, 189, 282
Bsc4I CCNNNNNNNGG 1 cut(s) 166
Bse1I ACTGG 3 cut(s) 184, 275, 379
BseBI CCWGG 1 cut(s) 161
BseDI CCNNGG 4 cut(s) 159, 160, 189, 282
BseGI GGATG 1 cut(s) 214
BseLI CCNNNNNNNGG 1 cut(s) 166
BseMII CTCAG 5 cut(s) 30, 86, 122, 246, 318
BseNI ACTGG 3 cut(s) 184, 275, 379
BseRI GAGGAG 3 cut(s) 33, 81, 84
BseXI GCAGC 5 cut(s) 88, 116, 253, 256, 303
BseYI CCCAGC 1 cut(s) 37
BsgI GTGCAG 1 cut(s) 42
BsiSI CCGG 1 cut(s) 420
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 1 cut(s) 166
BsmAI GTCTC 1 cut(s) 256
BsmFI GGGAC 1 cut(s) 263
BsmI GAATGC 2 cut(s) 332, 436
Bsp143I GATC 3 cut(s) 26, 125, 146
Bsp19I CCATGG 1 cut(s) 282
BspACI CCGC 1 cut(s) 68
BspCNI CTCAG 5 cut(s) 29, 85, 121, 247, 317
BspLI GGNNCC 1 cut(s) 222
BspMAI CTGCAG 1 cut(s) 106
BspMI ACCTGC 1 cut(s) 64
BspPI GGATC 2 cut(s) 133, 154
BsrI ACTGG 3 cut(s) 184, 275, 379
BssECI CCNNGG 4 cut(s) 159, 160, 189, 282
BssMI GATC 3 cut(s) 26, 125, 146
BssT1I CCWWGG 1 cut(s) 282
Bst2UI CCWGG 1 cut(s) 161
BstC8I GCNNGC 3 cut(s) 7, 34, 313
BstDEI CTNAG 6 cut(s) 16, 72, 108, 255, 304, 327
BstDSI CCRYGG 2 cut(s) 189, 282
BstF5I GGATG 1 cut(s) 214
BstKTI GATC 3 cut(s) 29, 128, 149
BstMAI GTCTC 1 cut(s) 256
BstMBI GATC 3 cut(s) 26, 125, 146
BstMWI GCNNNNNNNGC 5 cut(s) 38, 200, 209, 266, 300
BstNI CCWGG 1 cut(s) 161
BstNSI RCATGY 2 cut(s) 315, 427
BstSCI CCNGG 1 cut(s) 159
BstSFI CTRYAG 2 cut(s) 102, 246
BstV1I GCAGC 5 cut(s) 88, 116, 253, 256, 303
BstX2I RGATCY 1 cut(s) 125
BstYI RGATCY 1 cut(s) 125
BtgI CCRYGG 2 cut(s) 189, 282
BtsCI GGATG 1 cut(s) 214
BveI ACCTGC 1 cut(s) 64
Cac8I GCNNGC 3 cut(s) 7, 34, 313
Cfr13I GGNCC 1 cut(s) 221
CseI GACGC 1 cut(s) 334
Csp6I GTAC 1 cut(s) 177
CviAII CATG 7 cut(s) 50, 239, 283, 295, 312, 388, 424
CviQI GTAC 1 cut(s) 177
DdeI CTNAG 6 cut(s) 16, 72, 108, 255, 304, 327
DpnI GATC 3 cut(s) 28, 127, 148
DpnII GATC 3 cut(s) 26, 125, 146
Eco130I CCWWGG 1 cut(s) 282
Eco32I GATATC 1 cut(s) 367
Eco47I GGWCC 1 cut(s) 221
EcoO109I RGGNCCY 1 cut(s) 221
EcoRII CCWGG 1 cut(s) 159
EcoRV GATATC 1 cut(s) 367
EcoT14I CCWWGG 1 cut(s) 282
EcoT22I ATGCAT 1 cut(s) 313
ErhI CCWWGG 1 cut(s) 282
FaeI CATG 7 cut(s) 53, 242, 286, 298, 315, 391, 427
FaqI GGGAC 1 cut(s) 263
FatI CATG 7 cut(s) 49, 238, 282, 294, 311, 387, 423
FauI CCCGC 1 cut(s) 75
Fnu4HI GCNGC 5 cut(s) 102, 105, 267, 270, 292
FokI GGATG 1 cut(s) 221
Fsp4HI GCNGC 5 cut(s) 102, 105, 267, 270, 292
GluI GCNGC 5 cut(s) 102, 105, 267, 270, 292
GsaI CCCAGC 1 cut(s) 41
GsuI CTGGAG 2 cut(s) 81, 258
HapII CCGG 1 cut(s) 420
HgaI GACGC 1 cut(s) 334
Hin1II CATG 7 cut(s) 53, 242, 286, 298, 315, 391, 427
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HpaII CCGG 1 cut(s) 420
Hpy166II GTNNAC 1 cut(s) 349
Hpy188I TCNGA 3 cut(s) 256, 307, 403
Hpy188III TCNNGA 3 cut(s) 18, 114, 138
Hpy8I GTNNAC 1 cut(s) 349
HpyCH4V TGCA 6 cut(s) 53, 59, 104, 311, 315, 408
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 200, 209, 266, 300
HpyF3I CTNAG 6 cut(s) 16, 72, 108, 255, 304, 327
Hsp92II CATG 7 cut(s) 53, 242, 286, 298, 315, 391, 427
Kzo9I GATC 3 cut(s) 26, 125, 146
LmnI GCTCC 3 cut(s) 46, 94, 277
Lsp1109I GCAGC 5 cut(s) 88, 116, 253, 256, 303
LweI GCATC 2 cut(s) 199, 298
MalI GATC 3 cut(s) 28, 127, 148
MboI GATC 3 cut(s) 26, 125, 146
MflI RGATCY 1 cut(s) 125
MluCI AATT 2 cut(s) 141, 231
MnlI CCTC 8 cut(s) 14, 54, 81, 102, 105, 234, 250, 405
Mph1103I ATGCAT 1 cut(s) 313
MslI CAYNNNNRTG 1 cut(s) 392
MspA1I CMGCKG 1 cut(s) 101
MspI CCGG 1 cut(s) 420
MspR9I CCNGG 1 cut(s) 161
Mva1269I GAATGC 2 cut(s) 332, 436
MvaI CCWGG 1 cut(s) 161
MwoI GCNNNNNNNGC 5 cut(s) 38, 200, 209, 266, 300
NcoI CCATGG 1 cut(s) 282
NdeII GATC 3 cut(s) 26, 125, 146
NlaIII CATG 7 cut(s) 53, 242, 286, 298, 315, 391, 427
NlaIV GGNNCC 1 cut(s) 222
NsiI ATGCAT 1 cut(s) 313
NspI RCATGY 2 cut(s) 315, 427
PaeI GCATGC 1 cut(s) 315
PaqCI CACCTGC 1 cut(s) 64
PasI CCCWGGG 1 cut(s) 160
PctI GAATGC 2 cut(s) 332, 436
PflMI CCANNNNNTGG 1 cut(s) 166
PkrI GCNGC 5 cut(s) 103, 106, 268, 271, 293
PpuMI RGGWCCY 1 cut(s) 221
Psp5II RGGWCCY 1 cut(s) 221
Psp6I CCWGG 1 cut(s) 159
PspFI CCCAGC 1 cut(s) 37
PspGI CCWGG 1 cut(s) 159
PspN4I GGNNCC 1 cut(s) 222
PspPI GGNCC 1 cut(s) 221
PspPPI RGGWCCY 1 cut(s) 221
PstI CTGCAG 1 cut(s) 106
PsuI RGATCY 1 cut(s) 125
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 1 cut(s) 178
RsaNI GTAC 1 cut(s) 177
RseI CAYNNNNRTG 1 cut(s) 392
SatI GCNGC 5 cut(s) 102, 105, 267, 270, 292
Sau3AI GATC 3 cut(s) 26, 125, 146
Sau96I GGNCC 1 cut(s) 221
ScrFI CCNGG 1 cut(s) 161
SetI ASST 8 cut(s) 17, 25, 43, 58, 91, 103, 109, 247
SfaNI GCATC 2 cut(s) 199, 298
SfcI CTRYAG 2 cut(s) 102, 246
SinI GGWCC 1 cut(s) 221
SmiMI CAYNNNNRTG 1 cut(s) 392
SphI GCATGC 1 cut(s) 315
Sse9I AATT 2 cut(s) 141, 231
SsiI CCGC 1 cut(s) 68
StyD4I CCNGG 1 cut(s) 159
StyI CCWWGG 1 cut(s) 282
TaqI TCGA 1 cut(s) 25
TasI AATT 2 cut(s) 141, 231
TseI GCWGC 5 cut(s) 101, 104, 266, 269, 291
TspDTI ATGAA 1 cut(s) 255
Van91I CCANNNNNTGG 1 cut(s) 166
VpaK11BI GGWCC 1 cut(s) 221
XceI RCATGY 2 cut(s) 315, 427
Zsp2I ATGCAT 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.