FvH4_2g12400

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006914
Physical Location & Seq
Reverse (-)
10891143 .. 10893002
1860 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g12400.t1

Sequence Viewer

Length: 1134 bp
ATGCCAAAAAAAGTTTCAAAACTGGTGTGTTTGTATCTTTCTCTGATATTTGCTTCTGTATCTTCAGCTTTCGCAGGCACTACTGTTGATTGTCACTTCATACAAGATGTTAGTGACAATACGATTCGAGGGATGTTTGTGTTCGGAAGCTCTCTGGTGGACAATGGCAACAATAACTACTTGGAAACCACGTCCAAAGCCAATTACTTGCCATATGGAATCGACTTCCCATATGGGACTTCTGGGAGATTCACAAACAGTAAAAATGTGATTGATCTCCTTTGTGATCAGGTTAAGCTTCCCTTAATCCCACCATTTCAAGACCCCTCCGCTAAAGGAAGTAAAATTCTTCATGGTGTCAACCATGCCTCCGGCTCTTCTGGTATCCTTAATGATACAGGATCAGTTGCGGGTAATGCAACGAGCTTGAGTCAACAAATTATGGACTTTGAGGTGGTGACACTGGCAGAGTTAGAAGCACAGCTAGGGTGCAATAGCTCTCAATCGCTACCGAGCTACTTGATTGTTGTAGGAGTTGGTGGAAATGATTACATGCTCAACTATTTCCTTAGGAAATTGTACCTCCTTATCAGTATTGAAACCTTCACTGCAAATCTCATAGCCTCTCTTGATCAGAAACTCCAGAAATTGTACAGTCTGGGAGGTCGTAAATTTGTTGTAACGTCGCTAATGCCACTAGGCAATAGTCCTGTGATTACCAGGCCAAATATTATTGGCACTCCTTCAGGTCTCAACTACGCAGCCGAGCTCTACAACGCTCGCTTGAAGACATTGCTTGACTCTCTCAAAGCTCACTTATCTGACTTCAATTTCGTTTTAGTTAACCAATATAACATTATATCTAATCTAACACAGTCTCCTCCTTCCAGAACAGGCTTTAAAGACACACACAACCCTTGTTGTGCAGTTTCCCTGGTCTCTCAAGGTGGAAATGGAATATCATGCGATAGAGGTGGGAAGACATGTGTAGATAGGACGACTTATATGTATTTTGATGGGTTGCATCCGACAGAAGCTGTAAATGTTGAAATAGCAACCAAGGCGTATGCTTCTACTCTCCAGGGCGATGCTTACCCCATCAATGTCAGCCAACTCGTCCGGCTTCAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

378

Amino Acids

40.75

Weight (kDa)

6.98

Isoelectric Point (pI)

34.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 46 - 352 1.7e-23 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 330, 410
AclWI GGATC 1 cut(s) 409
AcsI RAATTY 3 cut(s) 345, 671, 1128
AcuI CTGAAG 2 cut(s) 48, 729
AfaI GTAC 2 cut(s) 581, 653
AflIII ACRYGT 1 cut(s) 983
AgsI TTSAA 7 cut(s) 18, 320, 599, 787, 829, 1049, 1127
AjiI CACGTC 1 cut(s) 192
AjnI CCWGG 3 cut(s) 719, 933, 1080
AjuI GAANNNNNNNTTGG 1 cut(s) 30
Alw21I GWGCWC 1 cut(s) 771
Alw26I GTCTC 3 cut(s) 755, 882, 943
AlwI GGATC 1 cut(s) 409
AlwNI CAGNNNCTG 1 cut(s) 1037
AoxI GGCC 1 cut(s) 722
ApeKI GCWGC 1 cut(s) 761
ApoI RAATTY 3 cut(s) 345, 671, 1128
ArsI GACNNNNNNTTYG 2 cut(s) 815, 847
AsuHPI GGTGA 1 cut(s) 469
AxyI CCTNAGG 1 cut(s) 569
BanII GRGCYC 1 cut(s) 771
BbsI GAAGAC 2 cut(s) 794, 986
Bbv12I GWGCWC 1 cut(s) 771
BbvI GCAGC 1 cut(s) 773
BccI CCATC 2 cut(s) 1010, 1106
BciT130I CCWGG 3 cut(s) 721, 935, 1082
BciVI GTATCC 1 cut(s) 395
BclI TGATCA 2 cut(s) 286, 631
BcoDI GTCTC 3 cut(s) 755, 882, 943
BfaI CTAG 2 cut(s) 485, 698
BfuI GTATCC 1 cut(s) 395
BisI GCNGC 1 cut(s) 762
BlsI GCNGC 1 cut(s) 763
Bme1390I CCNGG 3 cut(s) 721, 935, 1082
BmgBI CACGTC 1 cut(s) 192
BmrFI CCNGG 3 cut(s) 721, 935, 1082
BmsI GCATC 2 cut(s) 1033, 1078
BpiI GAAGAC 2 cut(s) 794, 986
BpmI CTGGAG 2 cut(s) 626, 1064
BpuEI CTTGAG 2 cut(s) 448, 927
BsaI GGTCTC 2 cut(s) 755, 943
BsaJI CCNNGG 3 cut(s) 933, 1059, 1081
BsaXI ACNNNNNCTCC 4 cut(s) 353, 383, 862, 892
Bse1I ACTGG 2 cut(s) 27, 468
Bse21I CCTNAGG 1 cut(s) 569
Bse3DI GCAATG 1 cut(s) 791
BseBI CCWGG 3 cut(s) 721, 935, 1082
BseDI CCNNGG 3 cut(s) 933, 1059, 1081
BseGI GGATG 2 cut(s) 138, 1024
BseMI GCAATG 1 cut(s) 791
BseNI ACTGG 2 cut(s) 27, 468
BseRI GAGGAG 1 cut(s) 870
BseXI GCAGC 1 cut(s) 773
BsgI GTGCAG 1 cut(s) 945
BshFI GGCC 1 cut(s) 724
BsiHKAI GWGCWC 1 cut(s) 771
BsiSI CCGG 2 cut(s) 372, 1120
BslFI GGGAC 1 cut(s) 250
BsmAI GTCTC 3 cut(s) 755, 882, 943
BsmFI GGGAC 1 cut(s) 250
BsnI GGCC 1 cut(s) 724
Bso31I GGTCTC 2 cut(s) 755, 943
Bsp1286I GDGCHC 1 cut(s) 771
Bsp1407I TGTACA 1 cut(s) 651
Bsp143I GATC 4 cut(s) 274, 286, 401, 631
BspACI CCGC 2 cut(s) 330, 410
BspANI GGCC 1 cut(s) 724
BspPI GGATC 1 cut(s) 409
BspQI GCTCTTC 1 cut(s) 382
BspTNI GGTCTC 2 cut(s) 755, 943
BsrDI GCAATG 1 cut(s) 791
BsrGI TGTACA 1 cut(s) 651
BsrI ACTGG 2 cut(s) 27, 468
BssECI CCNNGG 3 cut(s) 933, 1059, 1081
BssMI GATC 4 cut(s) 274, 286, 401, 631
BssT1I CCWWGG 1 cut(s) 1059
Bst2UI CCWGG 3 cut(s) 721, 935, 1082
Bst4CI ACNGT 4 cut(s) 85, 260, 656, 876
Bst6I CTCTTC 1 cut(s) 382
BstAUI TGTACA 1 cut(s) 651
BstC8I GCNNGC 2 cut(s) 76, 781
BstDEI CTNAG 1 cut(s) 569
BstF5I GGATG 2 cut(s) 138, 1024
BstKTI GATC 4 cut(s) 277, 289, 404, 634
BstMAI GTCTC 3 cut(s) 755, 882, 943
BstMBI GATC 4 cut(s) 274, 286, 401, 631
BstMWI GCNNNNNNNGC 2 cut(s) 416, 1061
BstNI CCWGG 3 cut(s) 721, 935, 1082
BstNSI RCATGY 2 cut(s) 556, 987
BstSCI CCNGG 3 cut(s) 719, 933, 1080
BstV1I GCAGC 1 cut(s) 773
BstV2I GAAGAC 2 cut(s) 794, 986
Bsu36I CCTNAGG 1 cut(s) 569
BsuI GTATCC 1 cut(s) 395
BsuRI GGCC 1 cut(s) 724
BtgZI GCGATG 1 cut(s) 1101
BtrI CACGTC 1 cut(s) 192
BtsCI GGATG 2 cut(s) 138, 1024
BtsI GCAGTG 1 cut(s) 606
BtsIMutI CAGTG 2 cut(s) 461, 606
Cac8I GCNNGC 2 cut(s) 76, 781
CaiI CAGNNNCTG 1 cut(s) 1037
Csp6I GTAC 2 cut(s) 580, 652
CviAII CATG 5 cut(s) 353, 365, 553, 963, 984
CviQI GTAC 2 cut(s) 580, 652
DdeI CTNAG 1 cut(s) 569
DpnI GATC 4 cut(s) 276, 288, 403, 633
DpnII GATC 4 cut(s) 274, 286, 401, 631
DraI TTTAAA 1 cut(s) 901
Eam1104I CTCTTC 1 cut(s) 382
EarI CTCTTC 1 cut(s) 382
Ecl136II GAGCTC 1 cut(s) 769
Eco130I CCWWGG 1 cut(s) 1059
Eco24I GRGCYC 1 cut(s) 771
Eco31I GGTCTC 2 cut(s) 755, 943
Eco53kI GAGCTC 1 cut(s) 769
Eco57I CTGAAG 2 cut(s) 48, 729
Eco81I CCTNAGG 1 cut(s) 569
EcoICRI GAGCTC 1 cut(s) 769
EcoRII CCWGG 3 cut(s) 719, 933, 1080
EcoT14I CCWWGG 1 cut(s) 1059
EcoT38I GRGCYC 1 cut(s) 771
ErhI CCWWGG 1 cut(s) 1059
FaeI CATG 5 cut(s) 356, 368, 556, 966, 987
FalI AAGNNNNNCTT 2 cut(s) 287, 319
FaqI GGGAC 1 cut(s) 250
FatI CATG 5 cut(s) 352, 364, 552, 962, 983
FauI CCCGC 1 cut(s) 403
FauNDI CATATG 2 cut(s) 214, 232
FbaI TGATCA 2 cut(s) 286, 631
Fnu4HI GCNGC 1 cut(s) 762
FokI GGATG 2 cut(s) 145, 1011
FriOI GRGCYC 1 cut(s) 771
Fsp4HI GCNGC 1 cut(s) 762
FspBI CTAG 2 cut(s) 485, 698
GluI GCNGC 1 cut(s) 762
GsuI CTGGAG 2 cut(s) 626, 1064
HaeIII GGCC 1 cut(s) 724
HapII CCGG 2 cut(s) 372, 1120
Hin1II CATG 5 cut(s) 356, 368, 556, 966, 987
HincII GTYRAC 3 cut(s) 361, 434, 844
HindII GTYRAC 3 cut(s) 361, 434, 844
HindIII AAGCTT 1 cut(s) 296
HinfI GANTC 5 cut(s) 124, 219, 249, 430, 800
HpaI GTTAAC 1 cut(s) 844
HpaII CCGG 2 cut(s) 372, 1120
HphI GGTGA 1 cut(s) 469
Hpy166II GTNNAC 4 cut(s) 160, 361, 434, 844
Hpy188I TCNGA 5 cut(s) 45, 146, 636, 823, 1029
Hpy188III TCNNGA 4 cut(s) 320, 629, 643, 888
Hpy8I GTNNAC 4 cut(s) 160, 361, 434, 844
Hpy99I CGWCG 1 cut(s) 688
HpyAV CCTTC 3 cut(s) 613, 753, 894
HpyCH4III ACNGT 4 cut(s) 85, 260, 656, 876
HpyCH4IV ACGT 2 cut(s) 191, 683
HpyCH4V TGCA 5 cut(s) 419, 492, 611, 926, 1024
HpyF10VI GCNNNNNNNGC 2 cut(s) 416, 1061
HpyF3I CTNAG 1 cut(s) 569
HpySE526I ACGT 2 cut(s) 191, 683
Hsp92II CATG 5 cut(s) 356, 368, 556, 966, 987
Ksp22I TGATCA 2 cut(s) 286, 631
KspAI GTTAAC 1 cut(s) 844
Kzo9I GATC 4 cut(s) 274, 286, 401, 631
LguI GCTCTTC 1 cut(s) 382
Lsp1109I GCAGC 1 cut(s) 773
LweI GCATC 2 cut(s) 1033, 1078
MaeI CTAG 2 cut(s) 485, 698
MaeII ACGT 2 cut(s) 191, 683
MaeIII GTNAC 4 cut(s) 92, 113, 457, 679
MalI GATC 4 cut(s) 276, 288, 403, 633
MboI GATC 4 cut(s) 274, 286, 401, 631
MboII GAAGA 5 cut(s) 54, 341, 369, 799, 991
MhlI GDGCHC 1 cut(s) 771
MluCI AATT 8 cut(s) 202, 345, 438, 575, 647, 671, 829, 1128
MlyI GAGTC 2 cut(s) 439, 794
MmeI TCCRAC 1 cut(s) 1052
MnlI CCTC 9 cut(s) 122, 337, 379, 445, 593, 634, 656, 891, 965
MseI TTAA 6 cut(s) 294, 305, 390, 843, 900, 1132
MspI CCGG 2 cut(s) 372, 1120
MspR9I CCNGG 3 cut(s) 721, 935, 1082
MvaI CCWGG 3 cut(s) 721, 935, 1082
MwoI GCNNNNNNNGC 2 cut(s) 416, 1061
NdeI CATATG 2 cut(s) 214, 232
NdeII GATC 4 cut(s) 274, 286, 401, 631
NlaIII CATG 5 cut(s) 356, 368, 556, 966, 987
NmeAIII GCCGAG 1 cut(s) 790
NmuCI GTSAC 3 cut(s) 92, 113, 457
NspI RCATGY 2 cut(s) 556, 987
PciI ACATGT 1 cut(s) 983
PciSI GCTCTTC 1 cut(s) 382
PfeI GAWTC 3 cut(s) 124, 219, 249
PkrI GCNGC 1 cut(s) 763
PleI GAGTC 2 cut(s) 438, 794
PpsI GAGTC 2 cut(s) 438, 794
PscI ACATGT 1 cut(s) 983
Psp124BI GAGCTC 1 cut(s) 771
Psp6I CCWGG 3 cut(s) 719, 933, 1080
PspGI CCWGG 3 cut(s) 719, 933, 1080
PstNI CAGNNNCTG 1 cut(s) 1037
RsaI GTAC 2 cut(s) 581, 653
RsaNI GTAC 2 cut(s) 580, 652
SacI GAGCTC 1 cut(s) 771
SapI GCTCTTC 1 cut(s) 382
SaqAI TTAA 6 cut(s) 294, 305, 390, 843, 900, 1132
SatI GCNGC 1 cut(s) 762
Sau3AI GATC 4 cut(s) 274, 286, 401, 631
SchI GAGTC 2 cut(s) 439, 794
ScrFI CCNGG 3 cut(s) 721, 935, 1082
SduI GDGCHC 1 cut(s) 771
SfaNI GCATC 2 cut(s) 1033, 1078
SmlI CTYRAG 2 cut(s) 427, 942
SmoI CTYRAG 2 cut(s) 427, 942
Sse9I AATT 8 cut(s) 202, 345, 438, 575, 647, 671, 829, 1128
SsiI CCGC 2 cut(s) 330, 410
SspI AATATT 1 cut(s) 730
SspMI CTAG 2 cut(s) 485, 698
SstI GAGCTC 1 cut(s) 771
StyD4I CCNGG 3 cut(s) 719, 933, 1080
StyI CCWWGG 1 cut(s) 1059
TaaI ACNGT 4 cut(s) 85, 260, 656, 876
TaiI ACGT 2 cut(s) 194, 686
TaqI TCGA 2 cut(s) 127, 222
TasI AATT 8 cut(s) 202, 345, 438, 575, 647, 671, 829, 1128
TatI WGTACW 1 cut(s) 651
TfiI GAWTC 3 cut(s) 124, 219, 249
Tru1I TTAA 6 cut(s) 294, 305, 390, 843, 900, 1132
Tru9I TTAA 6 cut(s) 294, 305, 390, 843, 900, 1132
TscAI CASTG 2 cut(s) 468, 613
TseFI GTSAC 3 cut(s) 92, 113, 457
TseI GCWGC 1 cut(s) 761
Tsp45I GTSAC 3 cut(s) 92, 113, 457
TspDTI ATGAA 2 cut(s) 88, 341
TspRI CASTG 2 cut(s) 468, 613
XapI RAATTY 3 cut(s) 345, 671, 1128
XceI RCATGY 2 cut(s) 556, 987
XspI CTAG 2 cut(s) 485, 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.