MD05G1039300.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006914
Physical Location & Seq
Forward (+)
6620725 .. 6623595
2871 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1039300.v1.1.491

Sequence Viewer

Length: 1140 bp
ATGCAATACCACTATCTGGAAAAACCATACAAACCACTTGTGGTTTTGTTCTCATTGTTTGCCTCTGTATCTCCAATCGGTTCCGATTGTGATTTCACAGAAGATCATGGGGCTGCAGCGACGATCGAAGGCATTTTTGTGTTTGGAAGCTCTTTGGTTGACAATGGCAACAATAACTTCCTCAAAAGCACGGCGAAAGCCGATTACTTGCCTTATGGAATAGATTTCCCAAGTGGATTACCTTCTGGGAGATTCACAAATGGTAAAAATGTGATTGACCTTCTTGGTGAACAACTGATGCTTCCTTCCTTCATCCCACCATTTAAGGACCGTTCAACCAAGGGGTCTAACATTGTTCATGGTGTCAACCATGCCTCTGGCTCTTCGGGTATCTTAGATGATACAGGCTTTGTTGGGGGAAATATTGTTATAAGTTTGAGTCAGCAAATCCGGGACTTTGAGGAGGTGACATTGCCAGAGTTAGAAGCTCAGCTGGCGTGCAATAGCTCTCAATCTCTTCCAAGATACTTGTTTGTTGTGGGAGTTGGTGGGAATGATTACATGTTCAACTATTTCCTCAAGAGATCCTACTTGCAAATTGATCTCCAAACCTTCACAGCAACTCTCATAGCATCTCTAGCTCAAAAACTCAAGAAATTGTACATTTTGGGAGGTCGAAAATTTGTGGTAATGTCGATAAATCCACTAGGGTACAGTCCTGTTGTTAACAGGCCAAATTATATTGGCAGTCCTCAAGCTCTGAACCAAGCAGCTCATCTGTACAACACTCAACTGAGGACACTGCTGGATGCTCTCAAAAGTGATATGCCTGACTTCAGTTTTGCTTTGGTCAATACATATAACATTATTACTAACATCATCCAGAATCCTACGTCCACAGGCTTTGAAGACACGAGCAATCCCTGTTGCAAAGTGAAATCTCAAATTGAAGGTGGAAATGGAGTATTATGCGAAAGAGGTGGAGAAACATGTGAGAACAGAAACAGGACGACAATTGTTTTCTTTGATGGGTTGCATCCAACGGAAGCGTTGAATGTTCAAATAGCAACCAAGGCTTATACTTCCACTCTTGAATTTCATGTTTATCCCATTAACGTCAAACAACTTGCCCAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

380

Amino Acids

41.58

Weight (kDa)

5.82

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 45 - 356 7.8e-25 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 431
AccB7I CCANNNNNTGG 1 cut(s) 16
AclWI GGATC 1 cut(s) 579
AcsI RAATTY 2 cut(s) 680, 1094
AcuI CTGAAG 1 cut(s) 820
AfaI GTAC 3 cut(s) 662, 713, 782
AfiI CCNNNNNNNGG 1 cut(s) 16
AflIII ACRYGT 2 cut(s) 561, 989
AgsI TTSAA 7 cut(s) 336, 568, 908, 950, 1054, 1061, 1094
AluBI AGCT 7 cut(s) 150, 488, 493, 507, 641, 758, 773
AluI AGCT 7 cut(s) 150, 488, 493, 507, 641, 758, 773
AlwI GGATC 1 cut(s) 579
AoxI GGCC 1 cut(s) 731
ApeKI GCWGC 3 cut(s) 113, 116, 770
ApoI RAATTY 2 cut(s) 680, 1094
ArsI GACNNNNNNTTYG 2 cut(s) 824, 856
AspS9I GGNCC 1 cut(s) 328
AsuC2I CCSGG 1 cut(s) 452
AsuHPI GGTGA 2 cut(s) 299, 478
AvaII GGWCC 1 cut(s) 328
BauI CACGAG 1 cut(s) 913
BbsI GAAGAC 1 cut(s) 915
BbvI GCAGC 3 cut(s) 100, 128, 782
BccI CCATC 1 cut(s) 1022
BceAI ACGGC 1 cut(s) 207
BcnI CCSGG 1 cut(s) 452
BfaI CTAG 2 cut(s) 638, 707
BfmI CTRYAG 1 cut(s) 114
BisI GCNGC 3 cut(s) 114, 117, 771
BlpI GCTNAGC 1 cut(s) 489
BlsI GCNGC 3 cut(s) 115, 118, 772
Bme1390I CCNGG 1 cut(s) 452
Bme18I GGWCC 1 cut(s) 328
BmgT120I GGNCC 1 cut(s) 328
BmiI GGNNCC 1 cut(s) 82
BmrFI CCNGG 1 cut(s) 452
BmsI GCATC 4 cut(s) 288, 641, 799, 1045
BpiI GAAGAC 1 cut(s) 915
Bpu1102I GCTNAGC 1 cut(s) 489
BpuEI CTTGAG 3 cut(s) 563, 635, 738
BpuMI CCSGG 1 cut(s) 452
BsaJI CCNNGG 2 cut(s) 339, 1071
Bsc4I CCNNNNNNNGG 1 cut(s) 16
Bse3DI GCAATG 1 cut(s) 470
BseDI CCNNGG 2 cut(s) 339, 1071
BseGI GGATG 4 cut(s) 312, 814, 879, 1036
BseLI CCNNNNNNNGG 1 cut(s) 16
BseMI GCAATG 1 cut(s) 470
BseMII CTCAG 2 cut(s) 503, 785
BseRI GAGGAG 1 cut(s) 476
BseXI GCAGC 3 cut(s) 100, 128, 782
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 1 cut(s) 733
BsiEI CGRYCG 1 cut(s) 126
BsiSI CCGG 1 cut(s) 451
BslFI GGGAC 1 cut(s) 467
BslI CCNNNNNNNGG 1 cut(s) 16
BsmFI GGGAC 1 cut(s) 467
BsnI GGCC 1 cut(s) 733
Bsp1407I TGTACA 2 cut(s) 660, 780
Bsp143I GATC 4 cut(s) 103, 123, 584, 601
Bsp1720I GCTNAGC 1 cut(s) 489
BspANI GGCC 1 cut(s) 733
BspCNI CTCAG 2 cut(s) 502, 786
BspLI GGNNCC 1 cut(s) 82
BspMAI CTGCAG 1 cut(s) 118
BspPI GGATC 1 cut(s) 579
BspQI GCTCTTC 1 cut(s) 388
BsrDI GCAATG 1 cut(s) 470
BsrGI TGTACA 2 cut(s) 660, 780
BssECI CCNNGG 2 cut(s) 339, 1071
BssMI GATC 4 cut(s) 103, 123, 584, 601
BssSI CACGAG 1 cut(s) 913
BssT1I CCWWGG 2 cut(s) 339, 1071
Bst2BI CACGAG 1 cut(s) 913
Bst4CI ACNGT 2 cut(s) 332, 716
Bst6I CTCTTC 2 cut(s) 388, 522
BstAUI TGTACA 2 cut(s) 660, 780
BstC8I GCNNGC 2 cut(s) 495, 499
BstDEI CTNAG 3 cut(s) 394, 489, 794
BstF5I GGATG 4 cut(s) 312, 814, 879, 1036
BstKTI GATC 4 cut(s) 106, 126, 587, 604
BstMBI GATC 4 cut(s) 103, 123, 584, 601
BstMCI CGRYCG 1 cut(s) 126
BstMWI GCNNNNNNNGC 3 cut(s) 494, 638, 1073
BstNSI RCATGY 2 cut(s) 565, 993
BstSCI CCNGG 1 cut(s) 450
BstSFI CTRYAG 1 cut(s) 114
BstV1I GCAGC 3 cut(s) 100, 128, 782
BstV2I GAAGAC 1 cut(s) 915
BstX2I RGATCY 1 cut(s) 584
BstXI CCANNNNNNTGG 1 cut(s) 377
BstYI RGATCY 1 cut(s) 584
BsuRI GGCC 1 cut(s) 733
BtsCI GGATG 4 cut(s) 312, 814, 879, 1036
BtsI GCAGTG 1 cut(s) 800
BtsIMutI CAGTG 1 cut(s) 800
Cac8I GCNNGC 2 cut(s) 495, 499
Cfr13I GGNCC 1 cut(s) 328
Csp6I GTAC 3 cut(s) 661, 712, 781
CspCI CAANNNNNGTGG 2 cut(s) 886, 921
CviAII CATG 6 cut(s) 107, 359, 371, 562, 990, 1100
CviQI GTAC 3 cut(s) 661, 712, 781
DdeI CTNAG 3 cut(s) 394, 489, 794
DpnI GATC 4 cut(s) 105, 125, 586, 603
DpnII GATC 4 cut(s) 103, 123, 584, 601
Eam1104I CTCTTC 2 cut(s) 388, 522
EarI CTCTTC 2 cut(s) 388, 522
Eco130I CCWWGG 2 cut(s) 339, 1071
Eco47I GGWCC 1 cut(s) 328
Eco57I CTGAAG 1 cut(s) 820
EcoT14I CCWWGG 2 cut(s) 339, 1071
ErhI CCWWGG 2 cut(s) 339, 1071
FaeI CATG 6 cut(s) 110, 362, 374, 565, 993, 1103
FaqI GGGAC 1 cut(s) 467
FatI CATG 6 cut(s) 106, 358, 370, 561, 989, 1099
Fnu4HI GCNGC 3 cut(s) 114, 117, 771
FokI GGATG 4 cut(s) 299, 821, 866, 1023
Fsp4HI GCNGC 3 cut(s) 114, 117, 771
FspBI CTAG 2 cut(s) 638, 707
GluI GCNGC 3 cut(s) 114, 117, 771
HaeIII GGCC 1 cut(s) 733
HapII CCGG 1 cut(s) 451
Hin1II CATG 6 cut(s) 110, 362, 374, 565, 993, 1103
HincII GTYRAC 3 cut(s) 160, 367, 727
HindII GTYRAC 3 cut(s) 160, 367, 727
HinfI GANTC 3 cut(s) 252, 439, 886
HpaI GTTAAC 1 cut(s) 727
HpaII CCGG 1 cut(s) 451
HphI GGTGA 2 cut(s) 299, 478
Hpy166II GTNNAC 5 cut(s) 160, 290, 367, 727, 897
Hpy188I TCNGA 2 cut(s) 85, 762
Hpy188III TCNNGA 5 cut(s) 17, 580, 652, 883, 1091
Hpy8I GTNNAC 5 cut(s) 160, 290, 367, 727, 897
Hpy99I CGWCG 1 cut(s) 124
HpyAV CCTTC 7 cut(s) 122, 252, 290, 315, 319, 622, 944
HpyCH4III ACNGT 2 cut(s) 332, 716
HpyCH4IV ACGT 2 cut(s) 893, 1116
HpyCH4V TGCA 6 cut(s) 4, 116, 501, 595, 930, 1036
HpyF10VI GCNNNNNNNGC 3 cut(s) 494, 638, 1073
HpyF3I CTNAG 3 cut(s) 394, 489, 794
HpySE526I ACGT 2 cut(s) 893, 1116
Hsp92II CATG 6 cut(s) 110, 362, 374, 565, 993, 1103
KspAI GTTAAC 1 cut(s) 727
Kzo9I GATC 4 cut(s) 103, 123, 584, 601
LguI GCTCTTC 1 cut(s) 388
Lsp1109I GCAGC 3 cut(s) 100, 128, 782
LweI GCATC 4 cut(s) 288, 641, 799, 1045
MaeI CTAG 2 cut(s) 638, 707
MaeII ACGT 2 cut(s) 893, 1116
MaeIII GTNAC 1 cut(s) 466
MalI GATC 4 cut(s) 105, 125, 586, 603
MboI GATC 4 cut(s) 103, 123, 584, 601
MboII GAAGA 4 cut(s) 113, 375, 509, 920
MfeI CAATTG 1 cut(s) 1014
MflI RGATCY 1 cut(s) 584
MluCI AATT 7 cut(s) 597, 656, 680, 736, 945, 1014, 1094
MlyI GAGTC 1 cut(s) 448
MmeI TCCRAC 1 cut(s) 1064
MseI TTAA 3 cut(s) 324, 726, 1113
MslI CAYNNNNRTG 1 cut(s) 137
MspA1I CMGCKG 1 cut(s) 493
MspI CCGG 1 cut(s) 451
MspR9I CCNGG 1 cut(s) 452
MunI CAATTG 1 cut(s) 1014
MwoI GCNNNNNNNGC 3 cut(s) 494, 638, 1073
NciI CCSGG 1 cut(s) 452
NdeII GATC 4 cut(s) 103, 123, 584, 601
NlaIII CATG 6 cut(s) 110, 362, 374, 565, 993, 1103
NlaIV GGNNCC 1 cut(s) 82
NmuCI GTSAC 1 cut(s) 466
NspI RCATGY 2 cut(s) 565, 993
PciI ACATGT 2 cut(s) 561, 989
PciSI GCTCTTC 1 cut(s) 388
PfeI GAWTC 2 cut(s) 252, 886
PflMI CCANNNNNTGG 1 cut(s) 16
PfoI TCCNGGA 1 cut(s) 450
PkrI GCNGC 3 cut(s) 115, 118, 772
Ple19I CGATCG 1 cut(s) 126
PleI GAGTC 1 cut(s) 447
PpsI GAGTC 1 cut(s) 447
PscI ACATGT 2 cut(s) 561, 989
PsiI TTATAA 1 cut(s) 431
PspN4I GGNNCC 1 cut(s) 82
PspPI GGNCC 1 cut(s) 328
PstI CTGCAG 1 cut(s) 118
PsuI RGATCY 1 cut(s) 584
PvuI CGATCG 1 cut(s) 126
PvuII CAGCTG 1 cut(s) 493
RsaI GTAC 3 cut(s) 662, 713, 782
RsaNI GTAC 3 cut(s) 661, 712, 781
RseI CAYNNNNRTG 1 cut(s) 137
SapI GCTCTTC 1 cut(s) 388
SaqAI TTAA 3 cut(s) 324, 726, 1113
SatI GCNGC 3 cut(s) 114, 117, 771
Sau3AI GATC 4 cut(s) 103, 123, 584, 601
Sau96I GGNCC 1 cut(s) 328
SchI GAGTC 1 cut(s) 448
ScrFI CCNGG 1 cut(s) 452
SfaNI GCATC 4 cut(s) 288, 641, 799, 1045
SfcI CTRYAG 1 cut(s) 114
SinI GGWCC 1 cut(s) 328
SmiMI CAYNNNNRTG 1 cut(s) 137
SmlI CTYRAG 3 cut(s) 578, 650, 753
SmoI CTYRAG 3 cut(s) 578, 650, 753
Sse9I AATT 7 cut(s) 597, 656, 680, 736, 945, 1014, 1094
SspI AATATT 1 cut(s) 424
SspMI CTAG 2 cut(s) 638, 707
StyD4I CCNGG 1 cut(s) 450
StyI CCWWGG 2 cut(s) 339, 1071
TaaI ACNGT 2 cut(s) 332, 716
TaiI ACGT 2 cut(s) 896, 1119
TaqI TCGA 3 cut(s) 126, 676, 695
TasI AATT 7 cut(s) 597, 656, 680, 736, 945, 1014, 1094
TatI WGTACW 2 cut(s) 660, 780
TfiI GAWTC 2 cut(s) 252, 886
Tru1I TTAA 3 cut(s) 324, 726, 1113
Tru9I TTAA 3 cut(s) 324, 726, 1113
TscAI CASTG 1 cut(s) 807
TseFI GTSAC 1 cut(s) 466
TseI GCWGC 3 cut(s) 113, 116, 770
Tsp45I GTSAC 1 cut(s) 466
TspDTI ATGAA 3 cut(s) 301, 347, 1088
TspGWI ACGGA 1 cut(s) 1058
TspRI CASTG 1 cut(s) 807
Van91I CCANNNNNTGG 1 cut(s) 16
VpaK11BI GGWCC 1 cut(s) 328
XapI RAATTY 2 cut(s) 680, 1094
XceI RCATGY 2 cut(s) 565, 993
XspI CTAG 2 cut(s) 638, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.