Rh6BG188700

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0006914
Physical Location & Seq
Reverse (-)
35046825 .. 35049560
2736 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG188700.1

Sequence Viewer

Length: 1092 bp
ATGGATTGGCCGTCGCATTTGGGATTCAGTGTGGGGAATAGGGAGCTGGAGCTGCAGCAAGCCCAGCCCGAGCCGCTTAAGAAGCTTCAAAATCTCTCTCTGATATTTGCTTCTGTATCTTCAGCTTCCGCAAGCACTGTTAATTGTAGCTTCAAACAACATGGTAGTGGGAATACGATCCAAGGGATGTTTGTGTTCGGAAGCTCTCTGGTGGATAATGGCAACAATTACTTCTTGGAAACCGAGTCGAAAGCCGATTACTTGCCGTATGGAATCGACTTCCCAAATGGAACTTCTGGGAGATTCACAAATGGTAAAAATGTTATTGATCTCCTTGGTGATCAGCTTAAGCTACCCTTCATTCCACCATTTCAAGACCCCTCCACTAAAGGAAGTAAAATTGTTCATGGTGTCAACCATGCCTCTGGCTCTACTGGTATCTTAGATGATACAGGATTAGTTCTGGGTAATGAACCGAGCTTGAGTCAACAAGTCAAGGACGTCGAGGCTGTGACACTGGCTGAGTTAGAAGCAGAGCTAGGGTGCAATAGCTCTCAATCTCTACCAAGCTACTTGATTGTTGTAGGAGTTGGTGGGAATGATTACATGCTCAACTATTTCCTTAGGAAATTGTACCTCCGAATCAGTATTGAAACCTTCACTGCAAATCTCATAGACTCTCTAGCTCAGAAACTCCAGGAATTGGACAATTTGGGAGGTCGTAAATTCGTGTTAACGTCACTAATGCCATTAGGCTACAGTCCTGTGATTAACAGGCCAAATCCTATTGGCACCGAATCAGCTTTGAATCAGGCAGCTCAGCTCTACAACGCTCACTTGAAGACATTGCTCGACTCTCTCAAAACTCACTTATCCGACTTCAATTTCGTTTTGGTTAACCAATATAACATTATATCTAATCTCACTTATTCTCCTCCTTCCACTACAGGCTTTAAAGACATAAGCAACCCTAGTTGTACAGTTTCCCTCTCTCAAGGTGGAATTGGAATATCATGCGATAGAGGTGGGAAGGCATGTGAAGATAGGACGGCTTATATGTATTTTGATGGGTTACACCAACAGAAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

363

Amino Acids

39.38

Weight (kDa)

5.37

Isoelectric Point (pI)

36.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 64 - 359 6e-21 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 504
AccB1I GGYRCC 1 cut(s) 791
AccB7I CCANNNNNTGG 1 cut(s) 703
AciI CCGC 2 cut(s) 74, 129
AclWI GGATC 1 cut(s) 172
AcoI YGGCCR 1 cut(s) 8
AcsI RAATTY 1 cut(s) 725
AcuI CTGAAG 1 cut(s) 105
AcyI GRCGYC 1 cut(s) 501
AfaI GTAC 2 cut(s) 635, 979
AfiI CCNNNNNNNGG 1 cut(s) 703
AflII CTTAAG 2 cut(s) 77, 347
AgsI TTSAA 7 cut(s) 89, 154, 374, 653, 808, 841, 883
AjnI CCWGG 1 cut(s) 696
AjuI GAANNNNNNNTTGG 2 cut(s) 277, 309
AlwI GGATC 1 cut(s) 172
AlwNI CAGNNNCTG 1 cut(s) 1087
Ama87I CYCGRG 1 cut(s) 68
AoxI GGCC 2 cut(s) 8, 776
ApeKI GCWGC 3 cut(s) 52, 55, 815
ApoI RAATTY 1 cut(s) 725
ArsI GACNNNNNNTTYG 2 cut(s) 869, 901
AsuHPI GGTGA 1 cut(s) 350
AvaI CYCGRG 1 cut(s) 68
AxyI CCTNAGG 1 cut(s) 623
BanI GGYRCC 1 cut(s) 791
BbsI GAAGAC 1 cut(s) 848
BbvI GCAGC 3 cut(s) 39, 67, 827
BccI CCATC 1 cut(s) 1061
BceAI ACGGC 2 cut(s) 250, 1065
BciT130I CCWGG 1 cut(s) 698
BclI TGATCA 1 cut(s) 340
BfaI CTAG 3 cut(s) 539, 683, 972
BfmI CTRYAG 3 cut(s) 53, 757, 945
BfrI CTTAAG 2 cut(s) 77, 347
BisI GCNGC 4 cut(s) 53, 56, 74, 816
BlpI GCTNAGC 1 cut(s) 819
BlsI GCNGC 4 cut(s) 54, 57, 75, 817
Bme1390I CCNGG 1 cut(s) 698
BmeT110I CYCGRG 1 cut(s) 68
BmiI GGNNCC 1 cut(s) 793
BmrFI CCNGG 1 cut(s) 698
BpiI GAAGAC 1 cut(s) 848
BpmI CTGGAG 2 cut(s) 68, 680
Bpu1102I GCTNAGC 1 cut(s) 819
BpuEI CTTGAG 2 cut(s) 502, 978
BsaHI GRCGYC 1 cut(s) 501
BsaJI CCNNGG 2 cut(s) 181, 334
BsaXI ACNNNNNCTCC 2 cut(s) 916, 946
Bsc4I CCNNNNNNNGG 1 cut(s) 703
Bse1I ACTGG 2 cut(s) 439, 522
Bse21I CCTNAGG 1 cut(s) 623
Bse3DI GCAATG 1 cut(s) 845
BseBI CCWGG 1 cut(s) 698
BseDI CCNNGG 2 cut(s) 181, 334
BseGI GGATG 1 cut(s) 192
BseLI CCNNNNNNNGG 1 cut(s) 703
BseMI GCAATG 1 cut(s) 845
BseMII CTCAG 3 cut(s) 513, 701, 833
BseNI ACTGG 2 cut(s) 439, 522
BseRI GAGGAG 1 cut(s) 924
BseXI GCAGC 3 cut(s) 39, 67, 827
BseYI CCCAGC 1 cut(s) 63
BshFI GGCC 2 cut(s) 10, 778
BshNI GGYRCC 1 cut(s) 791
BsiHKCI CYCGRG 1 cut(s) 68
BslI CCNNNNNNNGG 1 cut(s) 703
BsnI GGCC 2 cut(s) 10, 778
BsoBI CYCGRG 1 cut(s) 68
Bsp1407I TGTACA 1 cut(s) 977
Bsp143I GATC 3 cut(s) 177, 328, 340
Bsp1720I GCTNAGC 1 cut(s) 819
BspACI CCGC 2 cut(s) 74, 129
BspANI GGCC 2 cut(s) 10, 778
BspCNI CTCAG 3 cut(s) 514, 700, 832
BspLI GGNNCC 1 cut(s) 793
BspMAI CTGCAG 1 cut(s) 57
BspPI GGATC 1 cut(s) 172
BspT107I GGYRCC 1 cut(s) 791
BspTI CTTAAG 2 cut(s) 77, 347
BsrDI GCAATG 1 cut(s) 845
BsrGI TGTACA 1 cut(s) 977
BsrI ACTGG 2 cut(s) 439, 522
BssECI CCNNGG 2 cut(s) 181, 334
BssMI GATC 3 cut(s) 177, 328, 340
BssNI GRCGYC 1 cut(s) 501
BssT1I CCWWGG 2 cut(s) 181, 334
Bst2UI CCWGG 1 cut(s) 698
Bst4CI ACNGT 3 cut(s) 139, 761, 982
BstACI GRCGYC 1 cut(s) 501
BstAFI CTTAAG 2 cut(s) 77, 347
BstAUI TGTACA 1 cut(s) 977
BstC8I GCNNGC 2 cut(s) 60, 133
BstDEI CTNAG 5 cut(s) 442, 522, 623, 687, 819
BstF5I GGATG 1 cut(s) 192
BstKTI GATC 3 cut(s) 180, 331, 343
BstMBI GATC 3 cut(s) 177, 328, 340
BstMWI GCNNNNNNNGC 4 cut(s) 52, 64, 73, 82
BstNI CCWGG 1 cut(s) 698
BstNSI RCATGY 2 cut(s) 610, 1038
BstSCI CCNGG 1 cut(s) 696
BstSFI CTRYAG 3 cut(s) 53, 757, 945
BstV1I GCAGC 3 cut(s) 39, 67, 827
BstV2I GAAGAC 1 cut(s) 848
BstXI CCANNNNNNTGG 1 cut(s) 425
Bsu36I CCTNAGG 1 cut(s) 623
BsuRI GGCC 2 cut(s) 10, 778
BtsCI GGATG 1 cut(s) 192
BtsI GCAGTG 1 cut(s) 660
BtsIMutI CAGTG 4 cut(s) 34, 135, 515, 660
Cac8I GCNNGC 2 cut(s) 60, 133
CaiI CAGNNNCTG 1 cut(s) 1087
Csp6I GTAC 2 cut(s) 634, 978
CviAII CATG 6 cut(s) 161, 407, 419, 607, 1014, 1035
CviQI GTAC 2 cut(s) 634, 978
DdeI CTNAG 5 cut(s) 442, 522, 623, 687, 819
DpnI GATC 3 cut(s) 179, 330, 342
DpnII GATC 3 cut(s) 177, 328, 340
DraI TTTAAA 1 cut(s) 955
EaeI YGGCCR 1 cut(s) 8
Eco130I CCWWGG 2 cut(s) 181, 334
Eco57I CTGAAG 1 cut(s) 105
Eco81I CCTNAGG 1 cut(s) 623
Eco88I CYCGRG 1 cut(s) 68
EcoRII CCWGG 1 cut(s) 696
EcoT14I CCWWGG 2 cut(s) 181, 334
ErhI CCWWGG 2 cut(s) 181, 334
FaeI CATG 6 cut(s) 164, 410, 422, 610, 1017, 1038
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FatI CATG 6 cut(s) 160, 406, 418, 606, 1013, 1034
FbaI TGATCA 1 cut(s) 340
Fnu4HI GCNGC 4 cut(s) 53, 56, 74, 816
FokI GGATG 1 cut(s) 199
Fsp4HI GCNGC 4 cut(s) 53, 56, 74, 816
FspBI CTAG 3 cut(s) 539, 683, 972
GluI GCNGC 4 cut(s) 53, 56, 74, 816
GsaI CCCAGC 1 cut(s) 67
GsuI CTGGAG 2 cut(s) 68, 680
HaeIII GGCC 2 cut(s) 10, 778
Hin1I GRCGYC 1 cut(s) 501
Hin1II CATG 6 cut(s) 164, 410, 422, 610, 1017, 1038
HincII GTYRAC 4 cut(s) 415, 488, 735, 898
HindII GTYRAC 4 cut(s) 415, 488, 735, 898
HindIII AAGCTT 1 cut(s) 83
HpaI GTTAAC 2 cut(s) 735, 898
HphI GGTGA 1 cut(s) 350
Hpy166II GTNNAC 4 cut(s) 415, 488, 735, 898
Hpy188I TCNGA 5 cut(s) 102, 200, 641, 690, 877
Hpy188III TCNNGA 1 cut(s) 374
Hpy8I GTNNAC 4 cut(s) 415, 488, 735, 898
Hpy99I CGWCG 2 cut(s) 16, 506
HpyAV CCTTC 4 cut(s) 367, 667, 948, 1024
HpyCH4III ACNGT 3 cut(s) 139, 761, 982
HpyCH4IV ACGT 2 cut(s) 501, 737
HpyCH4V TGCA 3 cut(s) 55, 546, 665
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 64, 73, 82
HpyF3I CTNAG 5 cut(s) 442, 522, 623, 687, 819
HpySE526I ACGT 2 cut(s) 501, 737
Hsp92I GRCGYC 1 cut(s) 501
Hsp92II CATG 6 cut(s) 164, 410, 422, 610, 1017, 1038
Ksp22I TGATCA 1 cut(s) 340
KspAI GTTAAC 2 cut(s) 735, 898
Kzo9I GATC 3 cut(s) 177, 328, 340
LmnI GCTCC 2 cut(s) 43, 49
Lsp1109I GCAGC 3 cut(s) 39, 67, 827
MaeI CTAG 3 cut(s) 539, 683, 972
MaeII ACGT 2 cut(s) 501, 737
MaeIII GTNAC 3 cut(s) 511, 738, 1071
MalI GATC 3 cut(s) 179, 330, 342
MboI GATC 3 cut(s) 177, 328, 340
MboII GAAGA 3 cut(s) 111, 853, 1052
MluCI AATT 9 cut(s) 142, 226, 399, 629, 701, 709, 725, 883, 1002
MlyI GAGTC 4 cut(s) 254, 493, 671, 848
MmeI TCCRAC 1 cut(s) 900
MnlI CCTC 8 cut(s) 391, 433, 499, 647, 710, 945, 998, 1016
MseI TTAA 7 cut(s) 78, 141, 348, 734, 771, 897, 954
MslI CAYNNNNRTG 1 cut(s) 165
MspCI CTTAAG 2 cut(s) 77, 347
MspR9I CCNGG 1 cut(s) 698
MvaI CCWGG 1 cut(s) 698
MwoI GCNNNNNNNGC 4 cut(s) 52, 64, 73, 82
NdeII GATC 3 cut(s) 177, 328, 340
NlaIII CATG 6 cut(s) 164, 410, 422, 610, 1017, 1038
NlaIV GGNNCC 1 cut(s) 793
NmuCI GTSAC 2 cut(s) 511, 738
NspI RCATGY 2 cut(s) 610, 1038
PfeI GAWTC 6 cut(s) 24, 273, 303, 642, 797, 808
PflMI CCANNNNNTGG 1 cut(s) 703
PfoI TCCNGGA 1 cut(s) 696
PkrI GCNGC 4 cut(s) 54, 57, 75, 817
PleI GAGTC 4 cut(s) 253, 492, 671, 848
PpsI GAGTC 4 cut(s) 253, 492, 671, 848
Psp6I CCWGG 1 cut(s) 696
PspFI CCCAGC 1 cut(s) 63
PspGI CCWGG 1 cut(s) 696
PspN4I GGNNCC 1 cut(s) 793
PstI CTGCAG 1 cut(s) 57
PstNI CAGNNNCTG 1 cut(s) 1087
RsaI GTAC 2 cut(s) 635, 979
RsaNI GTAC 2 cut(s) 634, 978
RseI CAYNNNNRTG 1 cut(s) 165
SaqAI TTAA 7 cut(s) 78, 141, 348, 734, 771, 897, 954
SatI GCNGC 4 cut(s) 53, 56, 74, 816
Sau3AI GATC 3 cut(s) 177, 328, 340
SchI GAGTC 4 cut(s) 254, 493, 671, 848
ScrFI CCNGG 1 cut(s) 698
SfcI CTRYAG 3 cut(s) 53, 757, 945
SmiMI CAYNNNNRTG 1 cut(s) 165
SmlI CTYRAG 4 cut(s) 77, 347, 481, 993
SmoI CTYRAG 4 cut(s) 77, 347, 481, 993
Sse9I AATT 9 cut(s) 142, 226, 399, 629, 701, 709, 725, 883, 1002
SsiI CCGC 2 cut(s) 74, 129
SspMI CTAG 3 cut(s) 539, 683, 972
StyD4I CCNGG 1 cut(s) 696
StyI CCWWGG 2 cut(s) 181, 334
TaaI ACNGT 3 cut(s) 139, 761, 982
TaiI ACGT 2 cut(s) 504, 740
TaqI TCGA 4 cut(s) 248, 276, 504, 852
TasI AATT 9 cut(s) 142, 226, 399, 629, 701, 709, 725, 883, 1002
TatI WGTACW 1 cut(s) 977
TauI GCSGC 1 cut(s) 76
TfiI GAWTC 6 cut(s) 24, 273, 303, 642, 797, 808
Tru1I TTAA 7 cut(s) 78, 141, 348, 734, 771, 897, 954
Tru9I TTAA 7 cut(s) 78, 141, 348, 734, 771, 897, 954
TscAI CASTG 4 cut(s) 34, 142, 522, 667
TseFI GTSAC 2 cut(s) 511, 738
TseI GCWGC 3 cut(s) 52, 55, 815
Tsp45I GTSAC 2 cut(s) 511, 738
TspDTI ATGAA 3 cut(s) 349, 395, 486
TspRI CASTG 4 cut(s) 34, 142, 522, 667
Van91I CCANNNNNTGG 1 cut(s) 703
Vha464I CTTAAG 2 cut(s) 77, 347
XapI RAATTY 1 cut(s) 725
XceI RCATGY 2 cut(s) 610, 1038
XspI CTAG 3 cut(s) 539, 683, 972
ZraI GACGTC 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.