Rw6G016050

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0006914
Physical Location & Seq
Reverse (-)
31226683 .. 31228695
2013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G016050.1

Sequence Viewer

Length: 1074 bp
ATGACAAAAGAAGCTTCAAAACTGGTGTGTTTGTATCTCTCTCTGGTATTTGCTTCTGTATCTTCAGCTTCCGCAAACACTGTTACTTGTAGCTTCAAACAAGATGGTAGTGGGAATACGATCCAAGGGATGTTTGTATTCGGAAGCTCTCTGGTGGACAATGGCAACAATAACTTCTTGGAAACCAAGTCCAAAGCCGATTACTTGCCGTATGGAATCGACTTCCCAAATGGAACTTCTGGGAGATTCACAAATGGTAAAAATGTTATTGATCTCCTCGGTGATCAGCTTAAGCTTCCCTTCATTCCACCATTTCAAGACCCCTCCACCAAGGGAAGTAAAATTGTTCACGGTGTCAATCATGCCTCTGGCTCTTCTGGTATCTTAGATGATACAGGATTAGTTGCGGGTAATGCAACGAGCTTGAGTCAACAAGTTAAGGACTTTGAGGCGGTGACACTGGCAGAGTTAGAAGCAGAGCTAGGGTGCTGTAGCTCTCAATCGCTACCAAGCTACTTGATTGTTGTAGGAGTTGGTGGGAATGATTACATGCTCAACTATTTCCTTAGGAAATTGTACCTTCGAATCAACTTTGTTCTCTTCTTGTTGTTGCAGGAATTGTACATTTTGGGAGGTCGTAAATTCGTGTTAACGTCGCTAATGCCATTAGGCTACAGTCCTGTGATTAACAGGCCAAATCTTATTGGCACCCCATCAGCTCTGAATTACGCAGCTCAGCTCTACAACGCTCACTTGAAGACATTGCTCGACTCTCTCAAAACTCACTTATCCGACTTCAATTTCGTTTTGGTTAACCAATATAACATTATATCTAATCTCACTGACTCTCCTCCTTCCACTACAGGCTTTAAAGACATAAGCAACCCTTGTTGTATAGTTTCCCTCTCTCAAGGTGAGAAGGCATGTGAAGATAGGACGGCTTATATGTATTTTGATGGGTTACATCCAACAGAAGCTGTGAACGTTCAAATAGCAACCAAGGCATATGCTTCTACTCACCAGGGCGACGCTTACCCAATCAATGTCAGCCAACTTGCCCAGCTTCAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

357

Amino Acids

38.63

Weight (kDa)

5.67

Isoelectric Point (pI)

29.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 45 - 332 4.7e-21 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 707
AciI CCGC 3 cut(s) 72, 407, 452
AclI AACGTT 1 cut(s) 984
AclWI GGATC 1 cut(s) 115
AcsI RAATTY 2 cut(s) 641, 1068
AcuI CTGAAG 1 cut(s) 48
AfaI GTAC 2 cut(s) 578, 623
AflII CTTAAG 1 cut(s) 290
AgsI TTSAA 7 cut(s) 18, 97, 317, 757, 799, 989, 1067
AjnI CCWGG 1 cut(s) 1020
AjuI GAANNNNNNNTTGG 2 cut(s) 220, 252
AlwI GGATC 1 cut(s) 115
AlwNI CAGNNNCTG 1 cut(s) 977
AoxI GGCC 1 cut(s) 692
ApeKI GCWGC 1 cut(s) 731
ApoI RAATTY 2 cut(s) 641, 1068
ArsI GACNNNNNNTTYG 2 cut(s) 785, 817
AsuHPI GGTGA 4 cut(s) 293, 466, 926, 1010
AsuII TTCGAA 1 cut(s) 583
AxyI CCTNAGG 1 cut(s) 566
BanI GGYRCC 1 cut(s) 707
BbsI GAAGAC 1 cut(s) 764
BbvI GCAGC 1 cut(s) 743
BccI CCATC 3 cut(s) 98, 721, 950
BceAI ACGGC 2 cut(s) 193, 954
BciT130I CCWGG 1 cut(s) 1022
BclI TGATCA 1 cut(s) 283
BfaI CTAG 1 cut(s) 482
BfmI CTRYAG 3 cut(s) 490, 673, 861
BfrI CTTAAG 1 cut(s) 290
BisI GCNGC 1 cut(s) 732
BlpI GCTNAGC 1 cut(s) 735
BlsI GCNGC 1 cut(s) 733
Bme1390I CCNGG 1 cut(s) 1022
BmiI GGNNCC 1 cut(s) 709
BmrFI CCNGG 1 cut(s) 1022
BpiI GAAGAC 1 cut(s) 764
Bpu1102I GCTNAGC 1 cut(s) 735
Bpu14I TTCGAA 1 cut(s) 583
BpuEI CTTGAG 2 cut(s) 445, 894
BsaJI CCNNGG 5 cut(s) 124, 277, 330, 999, 1021
BsaXI ACNNNNNCTCC 2 cut(s) 832, 862
Bse1I ACTGG 2 cut(s) 27, 465
Bse21I CCTNAGG 1 cut(s) 566
Bse3DI GCAATG 1 cut(s) 761
BseBI CCWGG 1 cut(s) 1022
BseDI CCNNGG 5 cut(s) 124, 277, 330, 999, 1021
BseGI GGATG 2 cut(s) 135, 964
BseMI GCAATG 1 cut(s) 761
BseMII CTCAG 1 cut(s) 749
BseNI ACTGG 2 cut(s) 27, 465
BseRI GAGGAG 2 cut(s) 266, 840
BseXI GCAGC 1 cut(s) 743
BseYI CCCAGC 1 cut(s) 1059
BshFI GGCC 1 cut(s) 694
BshNI GGYRCC 1 cut(s) 707
BsnI GGCC 1 cut(s) 694
Bsp119I TTCGAA 1 cut(s) 583
Bsp1407I TGTACA 1 cut(s) 621
Bsp143I GATC 3 cut(s) 120, 271, 283
Bsp1720I GCTNAGC 1 cut(s) 735
BspACI CCGC 3 cut(s) 72, 407, 452
BspANI GGCC 1 cut(s) 694
BspCNI CTCAG 1 cut(s) 748
BspLI GGNNCC 1 cut(s) 709
BspPI GGATC 1 cut(s) 115
BspQI GCTCTTC 1 cut(s) 379
BspT104I TTCGAA 1 cut(s) 583
BspT107I GGYRCC 1 cut(s) 707
BspTI CTTAAG 1 cut(s) 290
BsrDI GCAATG 1 cut(s) 761
BsrGI TGTACA 1 cut(s) 621
BsrI ACTGG 2 cut(s) 27, 465
BssECI CCNNGG 5 cut(s) 124, 277, 330, 999, 1021
BssMI GATC 3 cut(s) 120, 271, 283
BssT1I CCWWGG 3 cut(s) 124, 330, 999
Bst2UI CCWGG 1 cut(s) 1022
Bst4CI ACNGT 3 cut(s) 82, 353, 677
Bst6I CTCTTC 2 cut(s) 379, 605
BstAFI CTTAAG 1 cut(s) 290
BstAUI TGTACA 1 cut(s) 621
BstBI TTCGAA 1 cut(s) 583
BstDEI CTNAG 3 cut(s) 385, 566, 735
BstF5I GGATG 2 cut(s) 135, 964
BstKTI GATC 3 cut(s) 123, 274, 286
BstMBI GATC 3 cut(s) 120, 271, 283
BstMWI GCNNNNNNNGC 2 cut(s) 413, 1001
BstNI CCWGG 1 cut(s) 1022
BstNSI RCATGY 2 cut(s) 553, 927
BstSCI CCNGG 1 cut(s) 1020
BstSFI CTRYAG 3 cut(s) 490, 673, 861
BstV1I GCAGC 1 cut(s) 743
BstV2I GAAGAC 1 cut(s) 764
Bsu36I CCTNAGG 1 cut(s) 566
BsuRI GGCC 1 cut(s) 694
BtsCI GGATG 2 cut(s) 135, 964
BtsIMutI CAGTG 3 cut(s) 78, 458, 840
CaiI CAGNNNCTG 1 cut(s) 977
CseI GACGC 1 cut(s) 1037
Csp6I GTAC 2 cut(s) 577, 622
CviAII CATG 3 cut(s) 362, 550, 924
CviQI GTAC 2 cut(s) 577, 622
DdeI CTNAG 3 cut(s) 385, 566, 735
DpnI GATC 3 cut(s) 122, 273, 285
DpnII GATC 3 cut(s) 120, 271, 283
DraI TTTAAA 1 cut(s) 871
Eam1104I CTCTTC 2 cut(s) 379, 605
EarI CTCTTC 2 cut(s) 379, 605
Eco130I CCWWGG 3 cut(s) 124, 330, 999
Eco57I CTGAAG 1 cut(s) 48
Eco81I CCTNAGG 1 cut(s) 566
EcoRII CCWGG 1 cut(s) 1020
EcoT14I CCWWGG 3 cut(s) 124, 330, 999
ErhI CCWWGG 3 cut(s) 124, 330, 999
FaeI CATG 3 cut(s) 365, 553, 927
FalI AAGNNNNNCTT 4 cut(s) 284, 316, 871, 903
FatI CATG 3 cut(s) 361, 549, 923
FauI CCCGC 1 cut(s) 400
FauNDI CATATG 1 cut(s) 1006
FbaI TGATCA 1 cut(s) 283
Fnu4HI GCNGC 1 cut(s) 732
FokI GGATG 2 cut(s) 142, 951
Fsp4HI GCNGC 1 cut(s) 732
FspBI CTAG 1 cut(s) 482
GluI GCNGC 1 cut(s) 732
GsaI CCCAGC 1 cut(s) 1063
HaeIII GGCC 1 cut(s) 694
HgaI GACGC 1 cut(s) 1037
Hin1II CATG 3 cut(s) 365, 553, 927
HincII GTYRAC 3 cut(s) 431, 651, 814
HindII GTYRAC 3 cut(s) 431, 651, 814
HindIII AAGCTT 2 cut(s) 12, 293
HinfI GANTC 6 cut(s) 216, 246, 427, 585, 770, 845
HpaI GTTAAC 2 cut(s) 651, 814
HphI GGTGA 4 cut(s) 293, 466, 926, 1010
Hpy166II GTNNAC 6 cut(s) 157, 349, 431, 651, 814, 982
Hpy188I TCNGA 3 cut(s) 143, 723, 793
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 6 cut(s) 157, 349, 431, 651, 814, 982
Hpy99I CGWCG 2 cut(s) 658, 1031
HpyAV CCTTC 4 cut(s) 310, 590, 864, 913
HpyCH4III ACNGT 3 cut(s) 82, 353, 677
HpyCH4IV ACGT 2 cut(s) 653, 984
HpyCH4V TGCA 2 cut(s) 416, 613
HpyF10VI GCNNNNNNNGC 2 cut(s) 413, 1001
HpyF3I CTNAG 3 cut(s) 385, 566, 735
HpySE526I ACGT 2 cut(s) 653, 984
Hsp92II CATG 3 cut(s) 365, 553, 927
Ksp22I TGATCA 1 cut(s) 283
KspAI GTTAAC 2 cut(s) 651, 814
Kzo9I GATC 3 cut(s) 120, 271, 283
LguI GCTCTTC 1 cut(s) 379
Lsp1109I GCAGC 1 cut(s) 743
MaeI CTAG 1 cut(s) 482
MaeII ACGT 2 cut(s) 653, 984
MaeIII GTNAC 3 cut(s) 82, 454, 960
MalI GATC 3 cut(s) 122, 273, 285
MboI GATC 3 cut(s) 120, 271, 283
MboII GAAGA 5 cut(s) 54, 366, 592, 769, 941
MluCI AATT 7 cut(s) 342, 572, 617, 641, 724, 799, 1068
MlyI GAGTC 3 cut(s) 436, 764, 839
MmeI TCCRAC 2 cut(s) 816, 992
MnlI CCTC 7 cut(s) 287, 334, 376, 442, 626, 861, 914
MseI TTAA 7 cut(s) 291, 438, 650, 687, 813, 870, 1072
MspCI CTTAAG 1 cut(s) 290
MspR9I CCNGG 1 cut(s) 1022
MvaI CCWGG 1 cut(s) 1022
MwoI GCNNNNNNNGC 2 cut(s) 413, 1001
NdeI CATATG 1 cut(s) 1006
NdeII GATC 3 cut(s) 120, 271, 283
NlaIII CATG 3 cut(s) 365, 553, 927
NlaIV GGNNCC 1 cut(s) 709
NmuCI GTSAC 1 cut(s) 454
NspI RCATGY 2 cut(s) 553, 927
NspV TTCGAA 1 cut(s) 583
PciSI GCTCTTC 1 cut(s) 379
PfeI GAWTC 3 cut(s) 216, 246, 585
PkrI GCNGC 1 cut(s) 733
PleI GAGTC 3 cut(s) 435, 764, 839
PpsI GAGTC 3 cut(s) 435, 764, 839
Psp1406I AACGTT 1 cut(s) 984
Psp6I CCWGG 1 cut(s) 1020
PspFI CCCAGC 1 cut(s) 1059
PspGI CCWGG 1 cut(s) 1020
PspN4I GGNNCC 1 cut(s) 709
PstNI CAGNNNCTG 1 cut(s) 977
RsaI GTAC 2 cut(s) 578, 623
RsaNI GTAC 2 cut(s) 577, 622
SapI GCTCTTC 1 cut(s) 379
SaqAI TTAA 7 cut(s) 291, 438, 650, 687, 813, 870, 1072
SatI GCNGC 1 cut(s) 732
Sau3AI GATC 3 cut(s) 120, 271, 283
SchI GAGTC 3 cut(s) 436, 764, 839
ScrFI CCNGG 1 cut(s) 1022
SfcI CTRYAG 3 cut(s) 490, 673, 861
SfuI TTCGAA 1 cut(s) 583
SmlI CTYRAG 3 cut(s) 290, 424, 909
SmoI CTYRAG 3 cut(s) 290, 424, 909
Sse9I AATT 7 cut(s) 342, 572, 617, 641, 724, 799, 1068
SsiI CCGC 3 cut(s) 72, 407, 452
SspMI CTAG 1 cut(s) 482
StyD4I CCNGG 1 cut(s) 1020
StyI CCWWGG 3 cut(s) 124, 330, 999
TaaI ACNGT 3 cut(s) 82, 353, 677
TaiI ACGT 2 cut(s) 656, 987
TaqI TCGA 3 cut(s) 219, 583, 768
TasI AATT 7 cut(s) 342, 572, 617, 641, 724, 799, 1068
TatI WGTACW 1 cut(s) 621
TfiI GAWTC 3 cut(s) 216, 246, 585
Tru1I TTAA 7 cut(s) 291, 438, 650, 687, 813, 870, 1072
Tru9I TTAA 7 cut(s) 291, 438, 650, 687, 813, 870, 1072
TscAI CASTG 3 cut(s) 85, 465, 847
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 1 cut(s) 731
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 1 cut(s) 292
TspRI CASTG 3 cut(s) 85, 465, 847
Vha464I CTTAAG 1 cut(s) 290
XapI RAATTY 2 cut(s) 641, 1068
XceI RCATGY 2 cut(s) 553, 927
XspI CTAG 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.