Rh6AG186300

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0006914
Physical Location & Seq
Reverse (-)
32734871 .. 32738083
3213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG186300.1

Sequence Viewer

Length: 1200 bp
ATGACAAAAGAAGCTTCAAAACTGGTGTGTTTGTATCTCTCTCTGATATTTGCTTCTGTATCTTCAGCTTCCGCAAGCACTGTTACTTGTAGCTTCAAACAAGATGGTAGTGGGAATACGATCCAAGGGATGTTTGTATTCGGAAGCTCTCTGGTGGACAATGGCAACAATAACTTCTTGGAAACCAAGTCCAAAGCCGATTACTTGCCGTATGGAATCGACTTCCCAAATGGAACTTCTGGGAGATTCACAAATGGTAAAAATGTTATTGATCTCCTCGGTGATCAGCTTAAGCTTCCCTTCATTCCACCATTTCAAGACCCCTCCACCAAGGGAAGTAAAATTGTTCATGGTGTCAATCATGCCTCTGGCTCTTCTGGTATCTTAGATGATACAGGATTAGTTGCGGGTAATGCAACGAGCTTGAGTCAACAAGTTAAGGACTTTGAGGCGGTGACACTGGCAGAGTTAGAAGCAGAGCTAGGGTGCAGTAGCTCTCAATCGCTACCAAGCTACTTGATTGTTGTAGGAGTTGGTGGGAATGATTACATGCTCAACTATTTCCTTAGGAAATTGTACCTTCGAATCAGTATTGAAACCTTCACTGCAAATCTCATAGCCTCTCTAGCTCAGAAACTCCAGGAATTGTACATTTTGGGAGGTCGTAAATTCGTGTTAACGTCGCTAATGCCATTAGGCTACAGTCCTGTGATTAACAGGCCAAATCTTATTGGCACCCCATCAGCTCTGAATTATGCAGCTCAGCTCTACAACGCTCACTTGAAGACATTGCTCGACTCTCTCAAAACTCACTTATCCGACTTCAATTTCGTTTTGGTTAACCAATATAACATTATATCTAATCTCACTTACTCTCCCCCTTCCACTACAGGCTTTAAAGACATAAGCAACCCTTGTTGTACAGTTTCCCTCTCTCAAGGTGGAAATGGAATCTCATGCGACAGAGGTGGGAAGGCATGTGAAGATAGGACGGCTTATATGTATTTTGATGGGTTACATCCAACAGAAGCTGTGAACGTTCAAATAGCAACCAAGGCATATGCTTGTACTGACCAGGGCGACGCTTACCCAATCAATATATGGCGAGGACTGTATACACGTATGATTTTCCGAGACTCAGCGCAAGAAGCTAATTTCCACAAAACCAGGCTCAATATTGTCAAGCACCGTCCCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

399

Amino Acids

43.38

Weight (kDa)

7.55

Isoelectric Point (pI)

34.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 45 - 350 2.5e-24 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 734
AccI GTMKAC 1 cut(s) 1115
AciI CCGC 3 cut(s) 72, 407, 452
AclI AACGTT 1 cut(s) 1038
AclWI GGATC 1 cut(s) 115
AcsI RAATTY 1 cut(s) 668
AcuI CTGAAG 1 cut(s) 48
AfaI GTAC 4 cut(s) 578, 650, 922, 1069
AflII CTTAAG 1 cut(s) 290
AflIII ACRYGT 1 cut(s) 1118
AgsI TTSAA 7 cut(s) 18, 97, 317, 596, 784, 826, 1043
AjnI CCWGG 3 cut(s) 639, 1074, 1166
AjuI GAANNNNNNNTTGG 2 cut(s) 220, 252
Alw26I GTCTC 1 cut(s) 1128
AlwI GGATC 1 cut(s) 115
AlwNI CAGNNNCTG 1 cut(s) 1031
AoxI GGCC 1 cut(s) 719
ApeKI GCWGC 1 cut(s) 758
ApoI RAATTY 1 cut(s) 668
ArsI GACNNNNNNTTYG 2 cut(s) 812, 844
AspLEI GCGC 1 cut(s) 1144
AsuHPI GGTGA 2 cut(s) 293, 466
AsuII TTCGAA 1 cut(s) 583
AxyI CCTNAGG 1 cut(s) 566
BanI GGYRCC 1 cut(s) 734
BbsI GAAGAC 1 cut(s) 791
BbvI GCAGC 1 cut(s) 770
BccI CCATC 3 cut(s) 98, 748, 1004
BceAI ACGGC 2 cut(s) 193, 1008
BciT130I CCWGG 3 cut(s) 641, 1076, 1168
BclI TGATCA 1 cut(s) 283
BcoDI GTCTC 1 cut(s) 1128
BfaI CTAG 2 cut(s) 482, 626
BfmI CTRYAG 2 cut(s) 700, 888
BfrI CTTAAG 1 cut(s) 290
BisI GCNGC 1 cut(s) 759
BlpI GCTNAGC 1 cut(s) 762
BlsI GCNGC 1 cut(s) 760
Bme1390I CCNGG 3 cut(s) 641, 1076, 1168
BmiI GGNNCC 1 cut(s) 736
BmrFI CCNGG 3 cut(s) 641, 1076, 1168
BpiI GAAGAC 1 cut(s) 791
BpmI CTGGAG 1 cut(s) 623
Bpu1102I GCTNAGC 1 cut(s) 762
Bpu14I TTCGAA 1 cut(s) 583
BpuEI CTTGAG 2 cut(s) 445, 921
BsaAI YACGTR 1 cut(s) 1121
BsaJI CCNNGG 5 cut(s) 124, 277, 330, 1053, 1075
BsaXI ACNNNNNCTCC 2 cut(s) 859, 889
Bse1I ACTGG 2 cut(s) 27, 465
Bse21I CCTNAGG 1 cut(s) 566
Bse3DI GCAATG 1 cut(s) 788
BseBI CCWGG 3 cut(s) 641, 1076, 1168
BseDI CCNNGG 5 cut(s) 124, 277, 330, 1053, 1075
BseGI GGATG 2 cut(s) 135, 1018
BseMI GCAATG 1 cut(s) 788
BseMII CTCAG 3 cut(s) 644, 776, 1152
BseNI ACTGG 2 cut(s) 27, 465
BseRI GAGGAG 1 cut(s) 266
BseXI GCAGC 1 cut(s) 770
BsgI GTGCAG 1 cut(s) 508
BshFI GGCC 1 cut(s) 721
BshNI GGYRCC 1 cut(s) 734
BslFI GGGAC 1 cut(s) 1176
BsmAI GTCTC 1 cut(s) 1128
BsmFI GGGAC 1 cut(s) 1176
BsnI GGCC 1 cut(s) 721
Bsp119I TTCGAA 1 cut(s) 583
Bsp1407I TGTACA 2 cut(s) 648, 920
Bsp143I GATC 3 cut(s) 120, 271, 283
Bsp1720I GCTNAGC 1 cut(s) 762
BspACI CCGC 3 cut(s) 72, 407, 452
BspANI GGCC 1 cut(s) 721
BspCNI CTCAG 3 cut(s) 643, 775, 1151
BspLI GGNNCC 1 cut(s) 736
BspPI GGATC 1 cut(s) 115
BspQI GCTCTTC 1 cut(s) 379
BspT104I TTCGAA 1 cut(s) 583
BspT107I GGYRCC 1 cut(s) 734
BspTI CTTAAG 1 cut(s) 290
BsrDI GCAATG 1 cut(s) 788
BsrGI TGTACA 2 cut(s) 648, 920
BsrI ACTGG 2 cut(s) 27, 465
BssECI CCNNGG 5 cut(s) 124, 277, 330, 1053, 1075
BssMI GATC 3 cut(s) 120, 271, 283
BssNAI GTATAC 1 cut(s) 1116
BssT1I CCWWGG 3 cut(s) 124, 330, 1053
Bst1107I GTATAC 1 cut(s) 1116
Bst2UI CCWGG 3 cut(s) 641, 1076, 1168
Bst4CI ACNGT 5 cut(s) 82, 704, 925, 1113, 1190
Bst6I CTCTTC 1 cut(s) 379
BstAFI CTTAAG 1 cut(s) 290
BstAUI TGTACA 2 cut(s) 648, 920
BstBAI YACGTR 1 cut(s) 1121
BstBI TTCGAA 1 cut(s) 583
BstC8I GCNNGC 1 cut(s) 76
BstDEI CTNAG 5 cut(s) 385, 566, 630, 762, 1138
BstF5I GGATG 2 cut(s) 135, 1018
BstHHI GCGC 1 cut(s) 1144
BstKTI GATC 3 cut(s) 123, 274, 286
BstMAI GTCTC 1 cut(s) 1128
BstMBI GATC 3 cut(s) 120, 271, 283
BstMWI GCNNNNNNNGC 4 cut(s) 413, 626, 1055, 1148
BstNI CCWGG 3 cut(s) 641, 1076, 1168
BstNSI RCATGY 2 cut(s) 553, 981
BstSCI CCNGG 3 cut(s) 639, 1074, 1166
BstSFI CTRYAG 2 cut(s) 700, 888
BstV1I GCAGC 1 cut(s) 770
BstV2I GAAGAC 1 cut(s) 791
BstZ17I GTATAC 1 cut(s) 1116
Bsu36I CCTNAGG 1 cut(s) 566
BsuRI GGCC 1 cut(s) 721
BtsCI GGATG 2 cut(s) 135, 1018
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 3 cut(s) 78, 458, 603
Cac8I GCNNGC 1 cut(s) 76
CaiI CAGNNNCTG 1 cut(s) 1031
CfoI GCGC 1 cut(s) 1144
CseI GACGC 1 cut(s) 1091
Csp6I GTAC 4 cut(s) 577, 649, 921, 1068
CviAII CATG 5 cut(s) 350, 362, 550, 957, 978
CviQI GTAC 4 cut(s) 577, 649, 921, 1068
DdeI CTNAG 5 cut(s) 385, 566, 630, 762, 1138
DpnI GATC 3 cut(s) 122, 273, 285
DpnII GATC 3 cut(s) 120, 271, 283
DraI TTTAAA 1 cut(s) 898
Eam1104I CTCTTC 1 cut(s) 379
EarI CTCTTC 1 cut(s) 379
Eco130I CCWWGG 3 cut(s) 124, 330, 1053
Eco57I CTGAAG 1 cut(s) 48
Eco81I CCTNAGG 1 cut(s) 566
EcoRII CCWGG 3 cut(s) 639, 1074, 1166
EcoT14I CCWWGG 3 cut(s) 124, 330, 1053
ErhI CCWWGG 3 cut(s) 124, 330, 1053
FaeI CATG 5 cut(s) 353, 365, 553, 960, 981
FalI AAGNNNNNCTT 4 cut(s) 284, 316, 898, 930
FaqI GGGAC 1 cut(s) 1176
FatI CATG 5 cut(s) 349, 361, 549, 956, 977
FauI CCCGC 1 cut(s) 400
FauNDI CATATG 1 cut(s) 1060
FbaI TGATCA 1 cut(s) 283
FblI GTMKAC 1 cut(s) 1115
Fnu4HI GCNGC 1 cut(s) 759
FokI GGATG 2 cut(s) 142, 1005
Fsp4HI GCNGC 1 cut(s) 759
FspBI CTAG 2 cut(s) 482, 626
GlaI GCGC 1 cut(s) 1143
GluI GCNGC 1 cut(s) 759
GsuI CTGGAG 1 cut(s) 623
HaeIII GGCC 1 cut(s) 721
HgaI GACGC 1 cut(s) 1091
HhaI GCGC 1 cut(s) 1144
Hin1II CATG 5 cut(s) 353, 365, 553, 960, 981
Hin6I GCGC 1 cut(s) 1142
HinP1I GCGC 1 cut(s) 1142
HincII GTYRAC 3 cut(s) 431, 678, 841
HindII GTYRAC 3 cut(s) 431, 678, 841
HindIII AAGCTT 2 cut(s) 12, 293
HinfI GANTC 7 cut(s) 216, 246, 427, 585, 797, 951, 1136
HpaI GTTAAC 2 cut(s) 678, 841
HphI GGTGA 2 cut(s) 293, 466
Hpy166II GTNNAC 6 cut(s) 157, 431, 678, 841, 1036, 1116
Hpy188I TCNGA 6 cut(s) 45, 143, 633, 750, 820, 1133
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 6 cut(s) 157, 431, 678, 841, 1036, 1116
Hpy99I CGWCG 2 cut(s) 685, 1085
HpyAV CCTTC 5 cut(s) 310, 590, 610, 891, 967
HpyCH4III ACNGT 5 cut(s) 82, 704, 925, 1113, 1190
HpyCH4IV ACGT 3 cut(s) 680, 1038, 1120
HpyCH4V TGCA 4 cut(s) 416, 489, 608, 758
HpyF10VI GCNNNNNNNGC 4 cut(s) 413, 626, 1055, 1148
HpyF3I CTNAG 5 cut(s) 385, 566, 630, 762, 1138
HpySE526I ACGT 3 cut(s) 680, 1038, 1120
Hsp92II CATG 5 cut(s) 353, 365, 553, 960, 981
HspAI GCGC 1 cut(s) 1142
Ksp22I TGATCA 1 cut(s) 283
KspAI GTTAAC 2 cut(s) 678, 841
Kzo9I GATC 3 cut(s) 120, 271, 283
LguI GCTCTTC 1 cut(s) 379
Lsp1109I GCAGC 1 cut(s) 770
MaeI CTAG 2 cut(s) 482, 626
MaeII ACGT 3 cut(s) 680, 1038, 1120
MaeIII GTNAC 3 cut(s) 82, 454, 1014
MalI GATC 3 cut(s) 122, 273, 285
MboI GATC 3 cut(s) 120, 271, 283
MboII GAAGA 4 cut(s) 54, 366, 796, 995
MluCI AATT 7 cut(s) 342, 572, 644, 668, 751, 826, 1153
MlyI GAGTC 3 cut(s) 436, 791, 1130
MmeI TCCRAC 2 cut(s) 843, 1046
MnlI CCTC 9 cut(s) 287, 334, 376, 442, 631, 653, 941, 959, 1100
MseI TTAA 6 cut(s) 291, 438, 677, 714, 840, 897
MspCI CTTAAG 1 cut(s) 290
MspR9I CCNGG 3 cut(s) 641, 1076, 1168
MvaI CCWGG 3 cut(s) 641, 1076, 1168
MwoI GCNNNNNNNGC 4 cut(s) 413, 626, 1055, 1148
NdeI CATATG 1 cut(s) 1060
NdeII GATC 3 cut(s) 120, 271, 283
NlaIII CATG 5 cut(s) 353, 365, 553, 960, 981
NlaIV GGNNCC 1 cut(s) 736
NmuCI GTSAC 1 cut(s) 454
NspI RCATGY 2 cut(s) 553, 981
NspV TTCGAA 1 cut(s) 583
PciSI GCTCTTC 1 cut(s) 379
PfeI GAWTC 4 cut(s) 216, 246, 585, 951
PfoI TCCNGGA 1 cut(s) 639
PkrI GCNGC 1 cut(s) 760
PleI GAGTC 3 cut(s) 435, 791, 1130
PpsI GAGTC 3 cut(s) 435, 791, 1130
Ppu21I YACGTR 1 cut(s) 1121
Psp1406I AACGTT 1 cut(s) 1038
Psp6I CCWGG 3 cut(s) 639, 1074, 1166
PspGI CCWGG 3 cut(s) 639, 1074, 1166
PspN4I GGNNCC 1 cut(s) 736
PstNI CAGNNNCTG 1 cut(s) 1031
RsaI GTAC 4 cut(s) 578, 650, 922, 1069
RsaNI GTAC 4 cut(s) 577, 649, 921, 1068
SapI GCTCTTC 1 cut(s) 379
SaqAI TTAA 6 cut(s) 291, 438, 677, 714, 840, 897
SatI GCNGC 1 cut(s) 759
Sau3AI GATC 3 cut(s) 120, 271, 283
SchI GAGTC 3 cut(s) 436, 791, 1130
ScrFI CCNGG 3 cut(s) 641, 1076, 1168
SfcI CTRYAG 2 cut(s) 700, 888
SfuI TTCGAA 1 cut(s) 583
SmlI CTYRAG 3 cut(s) 290, 424, 936
SmoI CTYRAG 3 cut(s) 290, 424, 936
Sse9I AATT 7 cut(s) 342, 572, 644, 668, 751, 826, 1153
SsiI CCGC 3 cut(s) 72, 407, 452
SspI AATATT 1 cut(s) 1177
SspMI CTAG 2 cut(s) 482, 626
StyD4I CCNGG 3 cut(s) 639, 1074, 1166
StyI CCWWGG 3 cut(s) 124, 330, 1053
TaaI ACNGT 5 cut(s) 82, 704, 925, 1113, 1190
TaiI ACGT 3 cut(s) 683, 1041, 1123
TaqI TCGA 3 cut(s) 219, 583, 795
TasI AATT 7 cut(s) 342, 572, 644, 668, 751, 826, 1153
TatI WGTACW 3 cut(s) 648, 920, 1067
TfiI GAWTC 4 cut(s) 216, 246, 585, 951
Tru1I TTAA 6 cut(s) 291, 438, 677, 714, 840, 897
Tru9I TTAA 6 cut(s) 291, 438, 677, 714, 840, 897
TscAI CASTG 3 cut(s) 85, 465, 610
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 1 cut(s) 758
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 2 cut(s) 292, 338
TspRI CASTG 3 cut(s) 85, 465, 610
Vha464I CTTAAG 1 cut(s) 290
XapI RAATTY 1 cut(s) 668
XceI RCATGY 2 cut(s) 553, 981
XcmI CCANNNNNNNNNTGG 1 cut(s) 1098
XmiI GTMKAC 1 cut(s) 1115
XspI CTAG 2 cut(s) 482, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.