Rmu_sc0004003.1_g000020

Belongs to the 'GDSL' lipolytic enzyme family

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004003.1
Physical Location & Seq
Reverse (-)
87932 .. 89819
1888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004003.1_g000020.1.cds

Sequence Viewer

Length: 1026 bp
atgtttgtattcggaagctctctggtggacaatggtaacaataacttcttggaaaccaagtccaaagccgattacttgccgtatggaatcgacttcccaaatggaacttctgggagattcacaaatggtaaaaatgttattgatctccttggtgatcagcttaagctacccttcattccaccatttcaagacccctccactaaaggaagtaaaattgttcatggtgtcaaccatgcctctggctctactggtatcttagatgatacaggattagttctgggtaatgaaccgagcttgagtcaacaagttaaggactttgaggcggtgacactggcagagttagaagcagagcttgggtgcagtagccctcaatcgctaccaagctacttgattgttgtaggagttggtggcatcatgctcaactatttccttaggaaattgtacctccgaatcagtattgaaaccttcactgcaaatctcatagactctctagctcagaaactccaggaattgtacaatttgggaggtagtaaattcgtgttaacgtcactaatgccattaggctacagtcctgtgattaacaggccaaatcctattggcaccgaatcagctctgaatcaggcagctcagctctacaacgctcacttgaagacattgctcggctctctcaaaactcacttatccgacttcaatttcgttttggttaaccaatataacattatatctaatctcacttattctcctccttccactactggtacttttagtatcattggctttatagacataagcgacccttgttgtacagtttccctctctcaaggtgcctacctttcaggtggaattggaatatcatgcgatagaggtgggaaggcatgtgaagataggacggcttatatgtattttgatgggttacatccaacagaagctgtaaatgttcaaatagcaaccaaggcatatgcttctactcaccagggtgaggcttacccaatcaatgtcagccaacttgcccagcttcaaatttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families

Protein Analysis

341

Amino Acids

36.7

Weight (kDa)

5.2

Isoelectric Point (pI)

33.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 599, 824
AciI CCGC 1 cut(s) 323
AcsI RAATTY 2 cut(s) 533, 1020
AfaI GTAC 4 cut(s) 443, 515, 760, 805
AfiI CCNNNNNNNGG 1 cut(s) 979
AflII CTTAAG 1 cut(s) 161
AgsI TTSAA 6 cut(s) 188, 461, 649, 691, 941, 1019
AjnI CCWGG 2 cut(s) 504, 972
AjuI GAANNNNNNNTTGG 4 cut(s) 91, 123, 336, 368
AleI CACNNNNGTG 1 cut(s) 975
AlwNI CAGNNNCTG 1 cut(s) 929
AoxI GGCC 1 cut(s) 584
ApeKI GCWGC 1 cut(s) 623
ApoI RAATTY 2 cut(s) 533, 1020
ArsI GACNNNNNNTTYG 2 cut(s) 677, 709
AsuHPI GGTGA 4 cut(s) 164, 337, 962, 989
AxyI CCTNAGG 1 cut(s) 431
BanI GGYRCC 2 cut(s) 599, 824
BbsI GAAGAC 1 cut(s) 656
BbvI GCAGC 1 cut(s) 635
BccI CCATC 1 cut(s) 902
BceAI ACGGC 2 cut(s) 64, 906
BciT130I CCWGG 2 cut(s) 506, 974
BclI TGATCA 1 cut(s) 154
BfaI CTAG 1 cut(s) 491
BfmI CTRYAG 1 cut(s) 565
BfrI CTTAAG 1 cut(s) 161
BisI GCNGC 1 cut(s) 624
BlpI GCTNAGC 1 cut(s) 627
BlsI GCNGC 1 cut(s) 625
Bme1390I CCNGG 2 cut(s) 506, 974
BmiI GGNNCC 2 cut(s) 601, 826
BmrFI CCNGG 2 cut(s) 506, 974
BmsI GCATC 1 cut(s) 420
BpiI GAAGAC 1 cut(s) 656
BpmI CTGGAG 1 cut(s) 488
Bpu1102I GCTNAGC 1 cut(s) 627
BpuEI CTTGAG 2 cut(s) 316, 804
BsaJI CCNNGG 3 cut(s) 148, 951, 973
BsaXI ACNNNNNCTCC 2 cut(s) 724, 754
Bsc4I CCNNNNNNNGG 1 cut(s) 979
Bse1I ACTGG 3 cut(s) 253, 336, 760
Bse21I CCTNAGG 1 cut(s) 431
Bse3DI GCAATG 1 cut(s) 653
BseBI CCWGG 2 cut(s) 506, 974
BseDI CCNNGG 3 cut(s) 148, 951, 973
BseGI GGATG 1 cut(s) 916
BseLI CCNNNNNNNGG 1 cut(s) 979
BseMI GCAATG 1 cut(s) 653
BseMII CTCAG 2 cut(s) 509, 641
BseNI ACTGG 3 cut(s) 253, 336, 760
BseRI GAGGAG 1 cut(s) 732
BseXI GCAGC 1 cut(s) 635
BseYI CCCAGC 1 cut(s) 1011
BsgI GTGCAG 1 cut(s) 379
BshFI GGCC 1 cut(s) 586
BshNI GGYRCC 2 cut(s) 599, 824
BslI CCNNNNNNNGG 1 cut(s) 979
BsnI GGCC 1 cut(s) 586
Bsp1407I TGTACA 2 cut(s) 513, 803
Bsp143I GATC 2 cut(s) 142, 154
Bsp1720I GCTNAGC 1 cut(s) 627
BspACI CCGC 1 cut(s) 323
BspANI GGCC 1 cut(s) 586
BspCNI CTCAG 2 cut(s) 508, 640
BspLI GGNNCC 2 cut(s) 601, 826
BspT107I GGYRCC 2 cut(s) 599, 824
BspTI CTTAAG 1 cut(s) 161
BsrDI GCAATG 1 cut(s) 653
BsrGI TGTACA 2 cut(s) 513, 803
BsrI ACTGG 3 cut(s) 253, 336, 760
BssECI CCNNGG 3 cut(s) 148, 951, 973
BssMI GATC 2 cut(s) 142, 154
BssT1I CCWWGG 2 cut(s) 148, 951
Bst2UI CCWGG 2 cut(s) 506, 974
Bst4CI ACNGT 2 cut(s) 569, 808
BstAFI CTTAAG 1 cut(s) 161
BstAUI TGTACA 2 cut(s) 513, 803
BstDEI CTNAG 4 cut(s) 256, 431, 495, 627
BstF5I GGATG 1 cut(s) 916
BstKTI GATC 2 cut(s) 145, 157
BstMBI GATC 2 cut(s) 142, 154
BstMWI GCNNNNNNNGC 1 cut(s) 953
BstNI CCWGG 2 cut(s) 506, 974
BstNSI RCATGY 1 cut(s) 879
BstSCI CCNGG 2 cut(s) 504, 972
BstSFI CTRYAG 1 cut(s) 565
BstV1I GCAGC 1 cut(s) 635
BstV2I GAAGAC 1 cut(s) 656
BstXI CCANNNNNNTGG 1 cut(s) 239
Bsu36I CCTNAGG 1 cut(s) 431
BsuRI GGCC 1 cut(s) 586
BtsCI GGATG 1 cut(s) 916
BtsI GCAGTG 1 cut(s) 468
BtsIMutI CAGTG 2 cut(s) 329, 468
CaiI CAGNNNCTG 1 cut(s) 929
Csp6I GTAC 4 cut(s) 442, 514, 759, 804
CviAII CATG 5 cut(s) 221, 233, 415, 855, 876
CviQI GTAC 4 cut(s) 442, 514, 759, 804
DdeI CTNAG 4 cut(s) 256, 431, 495, 627
DpnI GATC 2 cut(s) 144, 156
DpnII GATC 2 cut(s) 142, 154
Eco130I CCWWGG 2 cut(s) 148, 951
Eco81I CCTNAGG 1 cut(s) 431
EcoRII CCWGG 2 cut(s) 504, 972
EcoT14I CCWWGG 2 cut(s) 148, 951
ErhI CCWWGG 2 cut(s) 148, 951
FaeI CATG 5 cut(s) 224, 236, 418, 858, 879
FalI AAGNNNNNCTT 6 cut(s) 155, 187, 336, 368, 781, 813
FatI CATG 5 cut(s) 220, 232, 414, 854, 875
FauNDI CATATG 1 cut(s) 958
FbaI TGATCA 1 cut(s) 154
Fnu4HI GCNGC 1 cut(s) 624
FokI GGATG 1 cut(s) 903
Fsp4HI GCNGC 1 cut(s) 624
FspBI CTAG 1 cut(s) 491
GluI GCNGC 1 cut(s) 624
GsaI CCCAGC 1 cut(s) 1015
GsuI CTGGAG 1 cut(s) 488
HaeIII GGCC 1 cut(s) 586
Hin1II CATG 5 cut(s) 224, 236, 418, 858, 879
HincII GTYRAC 4 cut(s) 229, 302, 543, 706
HindII GTYRAC 4 cut(s) 229, 302, 543, 706
HinfI GANTC 7 cut(s) 87, 117, 298, 450, 485, 605, 616
HpaI GTTAAC 2 cut(s) 543, 706
HphI GGTGA 4 cut(s) 164, 337, 962, 989
Hpy166II GTNNAC 5 cut(s) 28, 229, 302, 543, 706
Hpy188I TCNGA 5 cut(s) 14, 449, 498, 615, 685
Hpy188III TCNNGA 1 cut(s) 188
Hpy8I GTNNAC 5 cut(s) 28, 229, 302, 543, 706
HpyAV CCTTC 4 cut(s) 181, 475, 756, 865
HpyCH4III ACNGT 2 cut(s) 569, 808
HpyCH4IV ACGT 1 cut(s) 545
HpyCH4V TGCA 2 cut(s) 360, 473
HpyF10VI GCNNNNNNNGC 1 cut(s) 953
HpyF3I CTNAG 4 cut(s) 256, 431, 495, 627
HpySE526I ACGT 1 cut(s) 545
Hsp92II CATG 5 cut(s) 224, 236, 418, 858, 879
Ksp22I TGATCA 1 cut(s) 154
KspAI GTTAAC 2 cut(s) 543, 706
Kzo9I GATC 2 cut(s) 142, 154
Lsp1109I GCAGC 1 cut(s) 635
LweI GCATC 1 cut(s) 420
MaeI CTAG 1 cut(s) 491
MaeII ACGT 1 cut(s) 545
MaeIII GTNAC 4 cut(s) 35, 325, 546, 912
MalI GATC 2 cut(s) 144, 156
MboI GATC 2 cut(s) 142, 154
MboII GAAGA 2 cut(s) 661, 893
MluCI AATT 8 cut(s) 213, 437, 509, 517, 533, 691, 843, 1020
MlyI GAGTC 2 cut(s) 307, 479
MmeI TCCRAC 2 cut(s) 708, 944
MseI TTAA 6 cut(s) 162, 309, 542, 579, 705, 1024
MslI CAYNNNNRTG 1 cut(s) 975
MspCI CTTAAG 1 cut(s) 161
MspR9I CCNGG 2 cut(s) 506, 974
MvaI CCWGG 2 cut(s) 506, 974
MwoI GCNNNNNNNGC 1 cut(s) 953
NdeI CATATG 1 cut(s) 958
NdeII GATC 2 cut(s) 142, 154
NlaIII CATG 5 cut(s) 224, 236, 418, 858, 879
NlaIV GGNNCC 2 cut(s) 601, 826
NmeAIII GCCGAG 1 cut(s) 639
NmuCI GTSAC 2 cut(s) 325, 546
NspI RCATGY 1 cut(s) 879
OliI CACNNNNGTG 1 cut(s) 975
PfeI GAWTC 5 cut(s) 87, 117, 450, 605, 616
PfoI TCCNGGA 1 cut(s) 504
PkrI GCNGC 1 cut(s) 625
PleI GAGTC 2 cut(s) 306, 479
PpsI GAGTC 2 cut(s) 306, 479
Psp6I CCWGG 2 cut(s) 504, 972
PspFI CCCAGC 1 cut(s) 1011
PspGI CCWGG 2 cut(s) 504, 972
PspN4I GGNNCC 2 cut(s) 601, 826
PstNI CAGNNNCTG 1 cut(s) 929
RsaI GTAC 4 cut(s) 443, 515, 760, 805
RsaNI GTAC 4 cut(s) 442, 514, 759, 804
RseI CAYNNNNRTG 1 cut(s) 975
SaqAI TTAA 6 cut(s) 162, 309, 542, 579, 705, 1024
SatI GCNGC 1 cut(s) 624
Sau3AI GATC 2 cut(s) 142, 154
SchI GAGTC 2 cut(s) 307, 479
ScrFI CCNGG 2 cut(s) 506, 974
SfaNI GCATC 1 cut(s) 420
SfcI CTRYAG 1 cut(s) 565
SmiMI CAYNNNNRTG 1 cut(s) 975
SmlI CTYRAG 3 cut(s) 161, 295, 819
SmoI CTYRAG 3 cut(s) 161, 295, 819
Sse9I AATT 8 cut(s) 213, 437, 509, 517, 533, 691, 843, 1020
SsiI CCGC 1 cut(s) 323
SspMI CTAG 1 cut(s) 491
StyD4I CCNGG 2 cut(s) 504, 972
StyI CCWWGG 2 cut(s) 148, 951
TaaI ACNGT 2 cut(s) 569, 808
TaiI ACGT 1 cut(s) 548
TaqI TCGA 1 cut(s) 90
TasI AATT 8 cut(s) 213, 437, 509, 517, 533, 691, 843, 1020
TatI WGTACW 2 cut(s) 513, 803
TfiI GAWTC 5 cut(s) 87, 117, 450, 605, 616
Tru1I TTAA 6 cut(s) 162, 309, 542, 579, 705, 1024
Tru9I TTAA 6 cut(s) 162, 309, 542, 579, 705, 1024
TscAI CASTG 2 cut(s) 336, 475
TseFI GTSAC 2 cut(s) 325, 546
TseI GCWGC 1 cut(s) 623
Tsp45I GTSAC 2 cut(s) 325, 546
TspDTI ATGAA 3 cut(s) 163, 209, 300
TspRI CASTG 2 cut(s) 336, 475
Vha464I CTTAAG 1 cut(s) 161
XapI RAATTY 2 cut(s) 533, 1020
XceI RCATGY 1 cut(s) 879
XspI CTAG 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.