Rroxscaffold_7G00196160

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0006914
Physical Location & Seq
Forward (+)
38830140 .. 38832408
2269 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00196160.1

Sequence Viewer

Length: 1155 bp
ATGACAAAAGAAGCTTCAAAACTGGTGTGTTTGTATCTCTCTCTGATATTTGCTTCTGTATGTTCAGCTTCCGCAAGCACTGTTACTTGTAGCTTCAAACAAGATGGTAGTGGGAATACGATCCAAGGGATGTTTGTATTCGGAAGCTCTCTGGTGGACAATGGCAACAATAACTTCTTGGAAACCAAGTCCAAAGCCGATTACTTGCCGTATGGAATCGACTTCTCAAATGGAACTTCTGGGAGATTCACAAATGGTAAAAATGTTATCGATCTCCTCGGTGATCAGCTTAAGCTTCCTTTCATTCCACCATTTCAAGACCCCTCCACTAAGGGAAGTAAAATTGTTCATGGTGTCAATCATGCCTCTGGCTCTTCTGGTATCTTAGATGATACAGGATTAGTTGCGGGTAATGCAACGAGCTTGAGTCAACAAGTTAAGGACTTTGAGGCGGTGACACTGGCAGAGTTAGAAGCAGAGCTAGGGTGCAGTAGCTCTCAATCGCTACCAAGCTACTTGATTGTTGTAGGAGTTGGTGGGAATGATTACATGCTCAACTATTTCCTTAGGAAATTGTACCTTCGAATCAGTATTGAAACCTTCACTGCAAATCTCATAGCCTCTCTAGCTCAGAAACTCCAGGAATTGTACATTTTGGGAGGTCGTAAATTCGTGTTAACGTCGCTAATGCCATTAGGCTACAGTCCTGTGATTAACAGGCCAAATCTTATTGGCACCCCATCAGCTCTGAATTCCGCAGCTCAGCTCTACAACGCTCACTTGAAGACTTTGCTCGACTCTCTCAAAACTCCCTTATCCGACTTCAATTTCGTTTTGGTTAACCAATATAACATTATATCTAATCTCACTTACTCTCCCACTTCCACTACAGGCTTTAAAGACATAAGCAACCCTTGTTGTACAGTTTCCCTCTCTCAAGGTGGAAATGGAATATCATGCGATAGAAATGGGAAGCCATGTGAAGATAGGACGGCTTATATGTATTTTGATGGGTTACATCCAACAGAAGCTGTGAACGTTCAAATAGCAACCAAGGCATATGCTTCTACTCACCAGGGCGACGCTTACCCAATCAATTTGGAGCTAGAGGTTCAAAAACTGGCATTGGCCGGGTTTGAGGCGCTGAACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

384

Amino Acids

41.25

Weight (kDa)

5.57

Isoelectric Point (pI)

25.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 45 - 350 1.2e-23 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 734
AciI CCGC 4 cut(s) 72, 407, 452, 756
AclI AACGTT 1 cut(s) 1038
AclWI GGATC 1 cut(s) 115
AcoI YGGCCR 1 cut(s) 1128
AcsI RAATTY 2 cut(s) 668, 751
AfaI GTAC 3 cut(s) 578, 650, 922
AflII CTTAAG 1 cut(s) 290
AgsI TTSAA 8 cut(s) 18, 97, 317, 596, 784, 826, 1043, 1115
AjnI CCWGG 2 cut(s) 639, 1074
AlwI GGATC 1 cut(s) 115
AlwNI CAGNNNCTG 1 cut(s) 1031
AoxI GGCC 2 cut(s) 719, 1128
ApeKI GCWGC 1 cut(s) 758
ApoI RAATTY 2 cut(s) 668, 751
ArsI GACNNNNNNTTYG 2 cut(s) 812, 844
AspLEI GCGC 1 cut(s) 1144
AsuC2I CCSGG 1 cut(s) 1132
AsuHPI GGTGA 3 cut(s) 293, 466, 1064
AsuII TTCGAA 1 cut(s) 583
AxyI CCTNAGG 1 cut(s) 566
BanI GGYRCC 1 cut(s) 734
BbsI GAAGAC 1 cut(s) 791
BbvI GCAGC 1 cut(s) 770
BccI CCATC 3 cut(s) 98, 748, 1004
BceAI ACGGC 2 cut(s) 193, 1008
BciT130I CCWGG 2 cut(s) 641, 1076
BclI TGATCA 1 cut(s) 283
BcnI CCSGG 1 cut(s) 1132
BfaI CTAG 3 cut(s) 482, 626, 1106
BfmI CTRYAG 2 cut(s) 700, 888
BfoI RGCGCY 1 cut(s) 1145
BfrI CTTAAG 1 cut(s) 290
BisI GCNGC 1 cut(s) 759
BlpI GCTNAGC 1 cut(s) 762
BlsI GCNGC 1 cut(s) 760
Bme1390I CCNGG 3 cut(s) 641, 1076, 1132
BmiI GGNNCC 1 cut(s) 736
BmrFI CCNGG 3 cut(s) 641, 1076, 1132
BpiI GAAGAC 1 cut(s) 791
BpmI CTGGAG 1 cut(s) 623
Bpu1102I GCTNAGC 1 cut(s) 762
Bpu14I TTCGAA 1 cut(s) 583
BpuEI CTTGAG 2 cut(s) 445, 921
BpuMI CCSGG 1 cut(s) 1132
Bsa29I ATCGAT 1 cut(s) 270
BsaJI CCNNGG 4 cut(s) 124, 277, 1053, 1075
BsaXI ACNNNNNCTCC 2 cut(s) 859, 889
Bse1I ACTGG 3 cut(s) 27, 465, 1125
Bse21I CCTNAGG 1 cut(s) 566
BseBI CCWGG 2 cut(s) 641, 1076
BseCI ATCGAT 1 cut(s) 270
BseDI CCNNGG 4 cut(s) 124, 277, 1053, 1075
BseGI GGATG 2 cut(s) 135, 1018
BseMII CTCAG 2 cut(s) 644, 776
BseNI ACTGG 3 cut(s) 27, 465, 1125
BseRI GAGGAG 1 cut(s) 266
BseXI GCAGC 1 cut(s) 770
BsgI GTGCAG 1 cut(s) 508
BshFI GGCC 2 cut(s) 721, 1130
BshNI GGYRCC 1 cut(s) 734
BshVI ATCGAT 1 cut(s) 270
BsiSI CCGG 1 cut(s) 1131
BsnI GGCC 2 cut(s) 721, 1130
Bsp119I TTCGAA 1 cut(s) 583
Bsp1407I TGTACA 2 cut(s) 648, 920
Bsp143I GATC 3 cut(s) 120, 271, 283
Bsp1720I GCTNAGC 1 cut(s) 762
BspACI CCGC 4 cut(s) 72, 407, 452, 756
BspANI GGCC 2 cut(s) 721, 1130
BspCNI CTCAG 2 cut(s) 643, 775
BspDI ATCGAT 1 cut(s) 270
BspLI GGNNCC 1 cut(s) 736
BspPI GGATC 1 cut(s) 115
BspQI GCTCTTC 1 cut(s) 379
BspT104I TTCGAA 1 cut(s) 583
BspT107I GGYRCC 1 cut(s) 734
BspTI CTTAAG 1 cut(s) 290
BsrGI TGTACA 2 cut(s) 648, 920
BsrI ACTGG 3 cut(s) 27, 465, 1125
BssECI CCNNGG 4 cut(s) 124, 277, 1053, 1075
BssMI GATC 3 cut(s) 120, 271, 283
BssT1I CCWWGG 2 cut(s) 124, 1053
Bst2UI CCWGG 2 cut(s) 641, 1076
Bst4CI ACNGT 3 cut(s) 82, 704, 925
Bst6I CTCTTC 1 cut(s) 379
BstAFI CTTAAG 1 cut(s) 290
BstAUI TGTACA 2 cut(s) 648, 920
BstBI TTCGAA 1 cut(s) 583
BstC8I GCNNGC 1 cut(s) 76
BstDEI CTNAG 5 cut(s) 330, 385, 566, 630, 762
BstF5I GGATG 2 cut(s) 135, 1018
BstH2I RGCGCY 1 cut(s) 1145
BstHHI GCGC 1 cut(s) 1144
BstKTI GATC 3 cut(s) 123, 274, 286
BstMBI GATC 3 cut(s) 120, 271, 283
BstMWI GCNNNNNNNGC 3 cut(s) 413, 626, 1055
BstNI CCWGG 2 cut(s) 641, 1076
BstNSI RCATGY 1 cut(s) 553
BstSCI CCNGG 3 cut(s) 639, 1074, 1130
BstSFI CTRYAG 2 cut(s) 700, 888
BstV1I GCAGC 1 cut(s) 770
BstV2I GAAGAC 1 cut(s) 791
Bsu15I ATCGAT 1 cut(s) 270
Bsu36I CCTNAGG 1 cut(s) 566
BsuRI GGCC 2 cut(s) 721, 1130
BsuTUI ATCGAT 1 cut(s) 270
BtsCI GGATG 2 cut(s) 135, 1018
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 3 cut(s) 78, 458, 603
Cac8I GCNNGC 1 cut(s) 76
CaiI CAGNNNCTG 1 cut(s) 1031
CfoI GCGC 1 cut(s) 1144
ClaI ATCGAT 1 cut(s) 270
CseI GACGC 1 cut(s) 1091
Csp6I GTAC 3 cut(s) 577, 649, 921
CviAII CATG 5 cut(s) 350, 362, 550, 957, 978
CviQI GTAC 3 cut(s) 577, 649, 921
DdeI CTNAG 5 cut(s) 330, 385, 566, 630, 762
DpnI GATC 3 cut(s) 122, 273, 285
DpnII GATC 3 cut(s) 120, 271, 283
DraI TTTAAA 1 cut(s) 898
EaeI YGGCCR 1 cut(s) 1128
Eam1104I CTCTTC 1 cut(s) 379
EarI CTCTTC 1 cut(s) 379
Eco130I CCWWGG 2 cut(s) 124, 1053
Eco81I CCTNAGG 1 cut(s) 566
EcoRI GAATTC 1 cut(s) 751
EcoRII CCWGG 2 cut(s) 639, 1074
EcoT14I CCWWGG 2 cut(s) 124, 1053
ErhI CCWWGG 2 cut(s) 124, 1053
FaeI CATG 5 cut(s) 353, 365, 553, 960, 981
FalI AAGNNNNNCTT 2 cut(s) 898, 930
FatI CATG 5 cut(s) 349, 361, 549, 956, 977
FauI CCCGC 1 cut(s) 400
FauNDI CATATG 1 cut(s) 1060
FbaI TGATCA 1 cut(s) 283
Fnu4HI GCNGC 1 cut(s) 759
FokI GGATG 2 cut(s) 142, 1005
Fsp4HI GCNGC 1 cut(s) 759
FspBI CTAG 3 cut(s) 482, 626, 1106
GlaI GCGC 1 cut(s) 1143
GluI GCNGC 1 cut(s) 759
GsuI CTGGAG 1 cut(s) 623
HaeII RGCGCY 1 cut(s) 1145
HaeIII GGCC 2 cut(s) 721, 1130
HapII CCGG 1 cut(s) 1131
HgaI GACGC 1 cut(s) 1091
HhaI GCGC 1 cut(s) 1144
Hin1II CATG 5 cut(s) 353, 365, 553, 960, 981
Hin6I GCGC 1 cut(s) 1142
HinP1I GCGC 1 cut(s) 1142
HincII GTYRAC 3 cut(s) 431, 678, 841
HindII GTYRAC 3 cut(s) 431, 678, 841
HindIII AAGCTT 2 cut(s) 12, 293
HinfI GANTC 5 cut(s) 216, 246, 427, 585, 797
HpaI GTTAAC 2 cut(s) 678, 841
HpaII CCGG 1 cut(s) 1131
HphI GGTGA 3 cut(s) 293, 466, 1064
Hpy166II GTNNAC 5 cut(s) 157, 431, 678, 841, 1036
Hpy188I TCNGA 6 cut(s) 45, 143, 633, 750, 820, 1154
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 5 cut(s) 157, 431, 678, 841, 1036
Hpy99I CGWCG 2 cut(s) 685, 1085
HpyAV CCTTC 2 cut(s) 590, 610
HpyCH4III ACNGT 3 cut(s) 82, 704, 925
HpyCH4IV ACGT 2 cut(s) 680, 1038
HpyCH4V TGCA 3 cut(s) 416, 489, 608
HpyF10VI GCNNNNNNNGC 3 cut(s) 413, 626, 1055
HpyF3I CTNAG 5 cut(s) 330, 385, 566, 630, 762
HpySE526I ACGT 2 cut(s) 680, 1038
Hsp92II CATG 5 cut(s) 353, 365, 553, 960, 981
HspAI GCGC 1 cut(s) 1142
Ksp22I TGATCA 1 cut(s) 283
KspAI GTTAAC 2 cut(s) 678, 841
Kzo9I GATC 3 cut(s) 120, 271, 283
LguI GCTCTTC 1 cut(s) 379
LmnI GCTCC 1 cut(s) 1102
Lsp1109I GCAGC 1 cut(s) 770
MaeI CTAG 3 cut(s) 482, 626, 1106
MaeII ACGT 2 cut(s) 680, 1038
MaeIII GTNAC 3 cut(s) 82, 454, 1014
MalI GATC 3 cut(s) 122, 273, 285
MboI GATC 3 cut(s) 120, 271, 283
MboII GAAGA 3 cut(s) 366, 796, 995
MluCI AATT 7 cut(s) 342, 572, 644, 668, 751, 826, 1096
MlyI GAGTC 2 cut(s) 436, 791
MmeI TCCRAC 2 cut(s) 843, 1046
MnlI CCTC 9 cut(s) 287, 334, 376, 442, 631, 653, 941, 1102, 1132
MseI TTAA 6 cut(s) 291, 438, 677, 714, 840, 897
MspCI CTTAAG 1 cut(s) 290
MspI CCGG 1 cut(s) 1131
MspR9I CCNGG 3 cut(s) 641, 1076, 1132
MvaI CCWGG 2 cut(s) 641, 1076
MwoI GCNNNNNNNGC 3 cut(s) 413, 626, 1055
NciI CCSGG 1 cut(s) 1132
NdeI CATATG 1 cut(s) 1060
NdeII GATC 3 cut(s) 120, 271, 283
NlaIII CATG 5 cut(s) 353, 365, 553, 960, 981
NlaIV GGNNCC 1 cut(s) 736
NmuCI GTSAC 1 cut(s) 454
NspI RCATGY 1 cut(s) 553
NspV TTCGAA 1 cut(s) 583
PciSI GCTCTTC 1 cut(s) 379
PfeI GAWTC 3 cut(s) 216, 246, 585
PfoI TCCNGGA 1 cut(s) 639
PkrI GCNGC 1 cut(s) 760
PleI GAGTC 2 cut(s) 435, 791
PpsI GAGTC 2 cut(s) 435, 791
Psp1406I AACGTT 1 cut(s) 1038
Psp6I CCWGG 2 cut(s) 639, 1074
PspGI CCWGG 2 cut(s) 639, 1074
PspN4I GGNNCC 1 cut(s) 736
PstNI CAGNNNCTG 1 cut(s) 1031
RsaI GTAC 3 cut(s) 578, 650, 922
RsaNI GTAC 3 cut(s) 577, 649, 921
SapI GCTCTTC 1 cut(s) 379
SaqAI TTAA 6 cut(s) 291, 438, 677, 714, 840, 897
SatI GCNGC 1 cut(s) 759
Sau3AI GATC 3 cut(s) 120, 271, 283
SchI GAGTC 2 cut(s) 436, 791
ScrFI CCNGG 3 cut(s) 641, 1076, 1132
SfcI CTRYAG 2 cut(s) 700, 888
SfuI TTCGAA 1 cut(s) 583
SmlI CTYRAG 3 cut(s) 290, 424, 936
SmoI CTYRAG 3 cut(s) 290, 424, 936
Sse9I AATT 7 cut(s) 342, 572, 644, 668, 751, 826, 1096
SsiI CCGC 4 cut(s) 72, 407, 452, 756
SspMI CTAG 3 cut(s) 482, 626, 1106
StyD4I CCNGG 3 cut(s) 639, 1074, 1130
StyI CCWWGG 2 cut(s) 124, 1053
TaaI ACNGT 3 cut(s) 82, 704, 925
TaiI ACGT 2 cut(s) 683, 1041
TaqI TCGA 4 cut(s) 219, 270, 583, 795
TasI AATT 7 cut(s) 342, 572, 644, 668, 751, 826, 1096
TatI WGTACW 2 cut(s) 648, 920
TfiI GAWTC 3 cut(s) 216, 246, 585
Tru1I TTAA 6 cut(s) 291, 438, 677, 714, 840, 897
Tru9I TTAA 6 cut(s) 291, 438, 677, 714, 840, 897
TscAI CASTG 3 cut(s) 85, 465, 610
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 1 cut(s) 758
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 2 cut(s) 292, 338
TspRI CASTG 3 cut(s) 85, 465, 610
Vha464I CTTAAG 1 cut(s) 290
XapI RAATTY 2 cut(s) 668, 751
XceI RCATGY 1 cut(s) 553
XspI CTAG 3 cut(s) 482, 626, 1106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.