FvH4_2g19180

Thiamine-repressible mitochondrial transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005884
Physical Location & Seq
Reverse (-)
16349793 .. 16355639
5847 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19180.t5

Sequence Viewer

Length: 1320 bp
ATGAAAAGTGAATCCTTGAAGTGGGTTTTAGGTTTGGTATACATATTTGCTGTTGCAGCTATTTGGATAGCTGCTAGTTTTGTAGTACAGTCGGTTGTGGATGCTGGTGTGTCCCCTTTTCTTGTTACCTACATATGCAATTCATTGTTTGTGATCTACATTCCCTTGGTTGAAGCCGGGCGGTATTTGGAGGATTCGTTTGGAGGGTTTTGGTTTTGGAAAAGTAAGGAGAGTAGTAGTCCTTTGCGAGAGTTGGTTGAGTCGGAGCAAACCACTCTTCTTGGAGGGGGTGGTGCTGTTGCGAAAAGTGATGATGCATCTGTGCATGTAGAAGAAGGAGAAATTGGTGGACATGGAAAGGTTATTGATGGAGAAGTAAAAGTTGTGGCGTATGAGCCTATTAGGAAATCAACAAATCTGTTTGAAATCAATGAAGTTCCTGATAAGCAGGTGGATGAAAAAGGCCGTTGGACACGGACTCGAGTGGCAAAAGTTAGCCTATTAATTTCCCCATTTTGGTTTTTTGCACAGCTCACTTTCAATTTGTCATTGAAGTATACTACAGTCACATCAAATACCATCTTGAGCAGTTCATCCAGCCTGTTCACCTTTTTGGTCTCTCTAGCATTCTTAGGTGAAAAGTTCACTTTGGTGAAGCTGTTCAGTGTTCTTCTTTGCATGGGAGGAACCATAGTTGTGAGCCTCGGTGACTCACGAACTACTCTAAGTTCAATTGCTTCAAACCCTCTACTAGGAGACATACTTGCTCTTTTCTCAGCAGCTTTCTATTCTGTGTATATTACCCTCATTCGCAAGAAGTTACCTGATGAGGATGATGAGGAAAATGGTCGTGCTAGTATGGCTCAGTTTTTGGGATTTTTAGGGCTTTCCAACCTCTTGATATTTCTTCCAGTTGCCCTTATACTGCATTTCTCAAAGTTGGAACCCTTTTATATGCTGACCTGGAAGCAGCTTGGTCTCATTGTTGGTAAAGGTTTGCTGGATAACGTTCTGAGCGATTACTTATGGGCCAAGGCTGTTCTTCTAACAACGACCACAGTAGCAACAGCTGGTCTATCAATACAAGTCCCATTGGCGGCAATTGTGGACTCAGTGACTGGCCACGCTCCTCATTTTGCTGATTACTTAGGAGCTGTGGCCGTTATGGTTGGATTTGTTGGCATTAATATTCCTTCTGATGCTTTTAAGAGGTCAAAAGGAGCTACACTAGAATTAGAGAATGGAAATAATAGAAATAGTAGTTCAATTAGTGAACCTGGTGGTTCATCAGGCAGTCCAGATTCAACTGCCCATTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

440

Amino Acids

47.39

Weight (kDa)

5.38

Isoelectric Point (pI)

33.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 143 - 272 1.6e-08 Purine nucleobase transmembrane transport
EamA PF00892 164 - 234 1.6e-09 EamA-like transporter family
TPT PF03151 170 - 317 2.9e-06 Triose-phosphate Transporter family
SLC35F PF06027 184 - 269 1.6e-07 Solute carrier family 35
EamA PF00892 251 - 396 6.1e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 439
Acc36I ACCTGC 1 cut(s) 439
AccI GTMKAC 2 cut(s) 39, 557
AciI CCGC 2 cut(s) 181, 1097
AclI AACGTT 1 cut(s) 1008
AcoI YGGCCR 2 cut(s) 1120, 1158
AfaI GTAC 1 cut(s) 87
AfiI CCNNNNNNNGG 4 cut(s) 21, 516, 752, 1096
AgsI TTSAA 9 cut(s) 19, 173, 425, 541, 553, 732, 741, 1266, 1305
AjnI CCWGG 2 cut(s) 962, 1276
AjuI GAANNNNNNNTTGG 2 cut(s) 327, 359
AleI CACNNNNGTG 1 cut(s) 650
AluBI AGCT 9 cut(s) 59, 71, 532, 658, 782, 973, 1070, 1154, 1223
AluI AGCT 9 cut(s) 59, 71, 532, 658, 782, 973, 1070, 1154, 1223
Alw26I GTCTC 3 cut(s) 622, 750, 983
AlwNI CAGNNNCTG 1 cut(s) 1118
Ama87I CYCGRG 1 cut(s) 480
AoxI GGCC 4 cut(s) 463, 1029, 1120, 1158
ApeKI GCWGC 4 cut(s) 56, 71, 779, 970
AseI ATTAAT 2 cut(s) 503, 1185
Asp700I GAANNNNTTC 1 cut(s) 659
AspS9I GGNCC 1 cut(s) 1029
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 4 cut(s) 598, 647, 664, 719
AvaI CYCGRG 1 cut(s) 480
BalI TGGCCA 1 cut(s) 1122
BbvI GCAGC 4 cut(s) 58, 68, 791, 982
BccI CCATC 2 cut(s) 362, 587
BceAI ACGGC 2 cut(s) 450, 1145
BciT130I CCWGG 2 cut(s) 964, 1278
BcnI CCSGG 1 cut(s) 178
BcoDI GTCTC 3 cut(s) 622, 750, 983
BfaI CTAG 5 cut(s) 75, 623, 752, 855, 1229
BfmI CTRYAG 1 cut(s) 561
BfuAI ACCTGC 1 cut(s) 439
BisI GCNGC 5 cut(s) 57, 72, 780, 971, 1098
BlsI GCNGC 5 cut(s) 58, 73, 781, 972, 1099
Bme1390I CCNGG 3 cut(s) 178, 964, 1278
BmeT110I CYCGRG 1 cut(s) 480
BmgT120I GGNCC 1 cut(s) 1029
BmiI GGNNCC 2 cut(s) 688, 945
BmrFI CCNGG 3 cut(s) 178, 964, 1278
BmsI GCATC 4 cut(s) 91, 304, 326, 1189
BpuEI CTTGAG 1 cut(s) 604
BpuMI CCSGG 1 cut(s) 178
BsaI GGTCTC 2 cut(s) 622, 983
BsaJI CCNNGG 3 cut(s) 165, 703, 1032
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 4 cut(s) 21, 516, 752, 1096
Bse1I ACTGG 2 cut(s) 911, 1123
BseBI CCWGG 2 cut(s) 964, 1278
BseDI CCNNGG 3 cut(s) 165, 703, 1032
BseGI GGATG 4 cut(s) 106, 460, 593, 838
BseLI CCNNNNNNNGG 4 cut(s) 21, 516, 752, 1096
BseMII CTCAG 4 cut(s) 789, 878, 1004, 1125
BseNI ACTGG 2 cut(s) 911, 1123
BseRI GAGGAG 1 cut(s) 1119
BseXI GCAGC 4 cut(s) 58, 68, 791, 982
BshFI GGCC 4 cut(s) 465, 1031, 1122, 1160
BsiHKCI CYCGRG 1 cut(s) 480
BsiSI CCGG 1 cut(s) 177
BslFI GGGAC 2 cut(s) 97, 1073
BslI CCNNNNNNNGG 4 cut(s) 21, 516, 752, 1096
BsmAI GTCTC 3 cut(s) 622, 750, 983
BsmFI GGGAC 2 cut(s) 97, 1073
BsmI GAATGC 1 cut(s) 626
BsnI GGCC 4 cut(s) 465, 1031, 1122, 1160
Bso31I GGTCTC 2 cut(s) 622, 983
BsoBI CYCGRG 1 cut(s) 480
Bsp143I GATC 1 cut(s) 153
BspACI CCGC 2 cut(s) 181, 1097
BspANI GGCC 4 cut(s) 465, 1031, 1122, 1160
BspCNI CTCAG 4 cut(s) 788, 877, 1005, 1124
BspLI GGNNCC 2 cut(s) 688, 945
BspMI ACCTGC 1 cut(s) 439
BspTNI GGTCTC 2 cut(s) 622, 983
BsrI ACTGG 2 cut(s) 911, 1123
BssECI CCNNGG 3 cut(s) 165, 703, 1032
BssMI GATC 1 cut(s) 153
BssNAI GTATAC 2 cut(s) 40, 558
BssT1I CCWWGG 2 cut(s) 165, 1032
Bst1107I GTATAC 2 cut(s) 40, 558
Bst2UI CCWGG 2 cut(s) 964, 1278
Bst4CI ACNGT 3 cut(s) 90, 565, 1060
Bst6I CTCTTC 1 cut(s) 282
BstDEI CTNAG 7 cut(s) 631, 725, 775, 864, 1013, 1111, 1147
BstENI CCTNNNNNAGG 1 cut(s) 750
BstF5I GGATG 4 cut(s) 106, 460, 593, 838
BstKTI GATC 1 cut(s) 156
BstMAI GTCTC 3 cut(s) 622, 750, 983
BstMBI GATC 1 cut(s) 153
BstMWI GCNNNNNNNGC 2 cut(s) 56, 860
BstNI CCWGG 2 cut(s) 964, 1278
BstNSI RCATGY 1 cut(s) 329
BstSCI CCNGG 3 cut(s) 176, 962, 1276
BstSFI CTRYAG 1 cut(s) 561
BstV1I GCAGC 4 cut(s) 58, 68, 791, 982
BstZ17I GTATAC 2 cut(s) 40, 558
BsuRI GGCC 4 cut(s) 465, 1031, 1122, 1160
BtsCI GGATG 4 cut(s) 106, 460, 593, 838
BtsIMutI CAGTG 2 cut(s) 670, 1119
BveI ACCTGC 1 cut(s) 439
CaiI CAGNNNCTG 1 cut(s) 1118
Cfr13I GGNCC 1 cut(s) 1029
CsiI ACCWGGT 1 cut(s) 1276
Csp6I GTAC 1 cut(s) 86
CspCI CAANNNNNGTGG 2 cut(s) 262, 297
CviAII CATG 3 cut(s) 326, 353, 679
CviQI GTAC 1 cut(s) 86
DdeI CTNAG 7 cut(s) 631, 725, 775, 864, 1013, 1111, 1147
DpnI GATC 1 cut(s) 155
DpnII GATC 1 cut(s) 153
EaeI YGGCCR 2 cut(s) 1120, 1158
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
Eco130I CCWWGG 2 cut(s) 165, 1032
Eco31I GGTCTC 2 cut(s) 622, 983
Eco88I CYCGRG 1 cut(s) 480
EcoNI CCTNNNNNAGG 1 cut(s) 750
EcoRII CCWGG 2 cut(s) 962, 1276
EcoT14I CCWWGG 2 cut(s) 165, 1032
EcoT22I ATGCAT 1 cut(s) 319
ErhI CCWWGG 2 cut(s) 165, 1032
FaeI CATG 3 cut(s) 329, 356, 682
FaqI GGGAC 2 cut(s) 97, 1073
FatI CATG 3 cut(s) 325, 352, 678
FauNDI CATATG 1 cut(s) 134
FblI GTMKAC 2 cut(s) 39, 557
Fnu4HI GCNGC 5 cut(s) 57, 72, 780, 971, 1098
FokI GGATG 4 cut(s) 113, 467, 580, 845
Fsp4HI GCNGC 5 cut(s) 57, 72, 780, 971, 1098
FspBI CTAG 5 cut(s) 75, 623, 752, 855, 1229
GluI GCNGC 5 cut(s) 57, 72, 780, 971, 1098
HaeIII GGCC 4 cut(s) 465, 1031, 1122, 1160
HapII CCGG 1 cut(s) 177
Hin1II CATG 3 cut(s) 329, 356, 682
HinfI GANTC 7 cut(s) 11, 194, 260, 478, 710, 1109, 1301
HpaII CCGG 1 cut(s) 177
HphI GGTGA 4 cut(s) 598, 647, 664, 719
Hpy166II GTNNAC 7 cut(s) 40, 350, 558, 606, 645, 1108, 1274
Hpy188I TCNGA 3 cut(s) 265, 1014, 1198
Hpy188III TCNNGA 5 cut(s) 440, 583, 714, 898, 1298
Hpy8I GTNNAC 7 cut(s) 40, 350, 558, 606, 645, 1108, 1274
HpyAV CCTTC 2 cut(s) 329, 1203
HpyCH4III ACNGT 3 cut(s) 90, 565, 1060
HpyCH4IV ACGT 1 cut(s) 1008
HpyCH4V TGCA 7 cut(s) 56, 138, 317, 325, 527, 678, 928
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 860
HpyF3I CTNAG 7 cut(s) 631, 725, 775, 864, 1013, 1111, 1147
HpySE526I ACGT 1 cut(s) 1008
Hsp92II CATG 3 cut(s) 329, 356, 682
Kzo9I GATC 1 cut(s) 153
LmnI GCTCC 4 cut(s) 265, 1132, 1151, 1220
Lsp1109I GCAGC 4 cut(s) 58, 68, 791, 982
LweI GCATC 4 cut(s) 91, 304, 326, 1189
MabI ACCWGGT 1 cut(s) 1276
MaeI CTAG 5 cut(s) 75, 623, 752, 855, 1229
MaeII ACGT 1 cut(s) 1008
MaeIII GTNAC 5 cut(s) 124, 565, 707, 819, 1114
MalI GATC 1 cut(s) 155
MboI GATC 1 cut(s) 153
MboII GAAGA 5 cut(s) 269, 344, 662, 899, 1034
MfeI CAATTG 2 cut(s) 732, 1101
MlsI TGGCCA 1 cut(s) 1122
MluCI AATT 8 cut(s) 139, 342, 504, 541, 732, 1101, 1232, 1266
MluNI TGGCCA 1 cut(s) 1122
MlyI GAGTC 4 cut(s) 269, 472, 704, 1103
MmeI TCCRAC 5 cut(s) 243, 449, 915, 921, 1150
Mox20I TGGCCA 1 cut(s) 1122
Mph1103I ATGCAT 1 cut(s) 319
MroXI GAANNNNTTC 1 cut(s) 659
MscI TGGCCA 1 cut(s) 1122
MseI TTAA 3 cut(s) 503, 1185, 1206
MslI CAYNNNNRTG 2 cut(s) 650, 695
Msp20I TGGCCA 1 cut(s) 1122
MspA1I CMGCKG 1 cut(s) 1070
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 3 cut(s) 178, 964, 1278
MunI CAATTG 2 cut(s) 732, 1101
Mva1269I GAATGC 1 cut(s) 626
MvaI CCWGG 2 cut(s) 964, 1278
MwoI GCNNNNNNNGC 2 cut(s) 56, 860
NciI CCSGG 1 cut(s) 178
NdeI CATATG 1 cut(s) 134
NdeII GATC 1 cut(s) 153
NlaIII CATG 3 cut(s) 329, 356, 682
NlaIV GGNNCC 2 cut(s) 688, 945
NmuCI GTSAC 3 cut(s) 565, 707, 1114
NsiI ATGCAT 1 cut(s) 319
NspI RCATGY 1 cut(s) 329
OliI CACNNNNGTG 1 cut(s) 650
PaeR7I CTCGAG 1 cut(s) 480
PaqCI CACCTGC 1 cut(s) 439
PcsI WCGNNNNNNNCGW 1 cut(s) 1014
PctI GAATGC 1 cut(s) 626
PdmI GAANNNNTTC 1 cut(s) 659
PfeI GAWTC 3 cut(s) 11, 194, 1301
PkrI GCNGC 5 cut(s) 58, 73, 781, 972, 1099
PleI GAGTC 4 cut(s) 268, 472, 704, 1103
PpsI GAGTC 4 cut(s) 268, 472, 704, 1103
PshBI ATTAAT 2 cut(s) 503, 1185
Psp1406I AACGTT 1 cut(s) 1008
Psp6I CCWGG 2 cut(s) 962, 1276
PspGI CCWGG 2 cut(s) 962, 1276
PspN4I GGNNCC 2 cut(s) 688, 945
PspPI GGNCC 1 cut(s) 1029
PspXI VCTCGAGB 1 cut(s) 480
PstNI CAGNNNCTG 1 cut(s) 1118
PvuII CAGCTG 1 cut(s) 1070
RsaI GTAC 1 cut(s) 87
RsaNI GTAC 1 cut(s) 86
RseI CAYNNNNRTG 2 cut(s) 650, 695
SaqAI TTAA 3 cut(s) 503, 1185, 1206
SatI GCNGC 5 cut(s) 57, 72, 780, 971, 1098
Sau3AI GATC 1 cut(s) 153
Sau96I GGNCC 1 cut(s) 1029
SchI GAGTC 4 cut(s) 269, 472, 704, 1103
ScrFI CCNGG 3 cut(s) 178, 964, 1278
SexAI ACCWGGT 1 cut(s) 1276
SfaNI GCATC 4 cut(s) 91, 304, 326, 1189
SfcI CTRYAG 1 cut(s) 561
Sfr274I CTCGAG 1 cut(s) 480
SlaI CTCGAG 1 cut(s) 480
SmiMI CAYNNNNRTG 2 cut(s) 650, 695
SmlI CTYRAG 2 cut(s) 480, 583
SmoI CTYRAG 2 cut(s) 480, 583
Sse9I AATT 8 cut(s) 139, 342, 504, 541, 732, 1101, 1232, 1266
SsiI CCGC 2 cut(s) 181, 1097
SspI AATATT 1 cut(s) 1189
SspMI CTAG 5 cut(s) 75, 623, 752, 855, 1229
StyD4I CCNGG 3 cut(s) 176, 962, 1276
StyI CCWWGG 2 cut(s) 165, 1032
TaaI ACNGT 3 cut(s) 90, 565, 1060
TaiI ACGT 1 cut(s) 1011
TaqI TCGA 1 cut(s) 481
TasI AATT 8 cut(s) 139, 342, 504, 541, 732, 1101, 1232, 1266
TatI WGTACW 1 cut(s) 85
TauI GCSGC 1 cut(s) 1100
TfiI GAWTC 3 cut(s) 11, 194, 1301
Tru1I TTAA 3 cut(s) 503, 1185, 1206
Tru9I TTAA 3 cut(s) 503, 1185, 1206
TscAI CASTG 2 cut(s) 670, 1119
TseFI GTSAC 3 cut(s) 565, 707, 1114
TseI GCWGC 4 cut(s) 56, 71, 779, 970
Tsp45I GTSAC 3 cut(s) 565, 707, 1114
TspDTI ATGAA 7 cut(s) 17, 132, 447, 471, 582, 1275, 1305
TspGWI ACGGA 1 cut(s) 490
TspRI CASTG 2 cut(s) 670, 1119
VspI ATTAAT 2 cut(s) 503, 1185
XagI CCTNNNNNAGG 1 cut(s) 750
XceI RCATGY 1 cut(s) 329
XhoI CTCGAG 1 cut(s) 480
XmiI GTMKAC 2 cut(s) 39, 557
XmnI GAANNNNTTC 1 cut(s) 659
XspI CTAG 5 cut(s) 75, 623, 752, 855, 1229
Zsp2I ATGCAT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.