MD04G1105300.v1.1

Thiamine-repressible mitochondrial transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005884
Physical Location & Seq
Forward (+)
19347497 .. 19348961
1465 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1105300.v1.1.491

Sequence Viewer

Length: 1089 bp
ATGAAAAGTGAAGTTGGGAAGTGGGTATTAGGTTTGGTATACATATTTGCTGTTGCATCTATCTGGATAGCTGCCAGTTTCGTAGTACAGTCGGTTGTAGATGCCGGTGTGTCCCCGTTCCTCATCACATACATATGCAATTCTCTGTTTGTGATCTACATTCCCTTGGTTGAAATCGGGCGGTATTTGGAGGATAATTTTGAGGGGTTTTGGATTTGGAGAAGTAGGAAGAGTAGCCCTTTGCAAAGATTAGGAGATTCGGAGCAAATCACTCTCCTTGAGGAGACTGACGGAGTTGTGAAAGCAGATGCGGAAGAGGGGGAAGTGAATCTTGGTGCAGAACCGAAAGTTGTATTGTACGAGCTTGTTGGAACTTCAGCAACTCAAGACAATGGCACTGAAACGGTAGATAAGCAAGTGGATGAAAAAGGGCGTTGGACACGAACTCGAGTGGCTAAATCGAATACAATCTTAAGCAGTTCATCCAGCCTTTTCACCTTTTTGGTCGCCCTAGTATTCTTAGGGGAGAAGTTTACTTTGGTTAAGCTTATTAGCGTTCTTCTTTGCATGGGAGGAACGATAATTGTCAGCCTTGGTGACTCACGAACTGCTCTAAGTGCAATTGCTTCAAACCCTCTCCTCGGCGACATACTTGCTCTTGTCTCGGCGGCCTTCTATTCTGTGTATATTACCCTCATTCGCAAGAAATTACCTGATGAGGATGATGAAAAAAGCGGCCGTGCCAGTATGGCTCAGTTTCTAGGATTTCTAGGGCTTTCCAACCTTCTTATATTTCTTCCGGTTGCCCTTATACTTCATTTCTCCAAGCTGGAGCCGTTTTACATGCTTACCTGGGAGCAGGTCGGTCTCATCGTTGGTAAAGGTTTGTTGGATAATGTTCTGAGCGACTACTTATGGGCCAAGGCCGTTCTTCTAACCACCACGACGGTAGCAACGGCCGGTCTGACCATCCAGGTTCCGCTGGCGGCAATTGTTGACTCAATGACTGGCCATGCTCCTCATTTTGCCCGATTACCTTGGAGCCGTGGCGGTCATGATCGGATTCGCAGGCATAAACATTTCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

363

Amino Acids

39.8

Weight (kDa)

6.11

Isoelectric Point (pI)

32.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SLC35F PF06027 158 - 264 1e-06 Solute carrier family 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 850
AccI GTMKAC 1 cut(s) 39
AciI CCGC 8 cut(s) 181, 311, 668, 735, 980, 986, 1050, 1084
AcoI YGGCCR 3 cut(s) 736, 957, 1009
AcuI CTGAAG 1 cut(s) 360
AfaI GTAC 2 cut(s) 87, 359
AfiI CCNNNNNNNGG 1 cut(s) 641
AflII CTTAAG 1 cut(s) 472
AgsI TTSAA 2 cut(s) 173, 630
AjnI CCWGG 2 cut(s) 851, 972
AjuI GAANNNNNNNTTGG 4 cut(s) 315, 347, 521, 553
AluBI AGCT 4 cut(s) 71, 364, 547, 829
AluI AGCT 4 cut(s) 71, 364, 547, 829
Alw26I GTCTC 3 cut(s) 278, 667, 872
Ama87I CYCGRG 1 cut(s) 447
AoxI GGCC 6 cut(s) 669, 736, 918, 924, 957, 1009
ApeKI GCWGC 1 cut(s) 71
AspS9I GGNCC 1 cut(s) 918
AsuHPI GGTGA 2 cut(s) 487, 608
AvaI CYCGRG 1 cut(s) 447
BalI TGGCCA 1 cut(s) 1011
BbvI GCAGC 1 cut(s) 58
BccI CCATC 1 cut(s) 977
BceAI ACGGC 5 cut(s) 723, 820, 911, 972, 1029
BcgI CGANNNNNNTGC 2 cut(s) 635, 669
BciT130I CCWGG 2 cut(s) 853, 974
BcoDI GTCTC 3 cut(s) 278, 667, 872
BfaI CTAG 3 cut(s) 512, 761, 770
BfrI CTTAAG 1 cut(s) 472
BfuAI ACCTGC 1 cut(s) 850
BglI GCCNNNNNGGC 1 cut(s) 749
BisI GCNGC 4 cut(s) 72, 669, 736, 987
BlsI GCNGC 4 cut(s) 73, 670, 737, 988
Bme1390I CCNGG 2 cut(s) 853, 974
BmeT110I CYCGRG 1 cut(s) 447
BmgT120I GGNCC 1 cut(s) 918
BmiI GGNNCC 3 cut(s) 834, 978, 1043
BmrFI CCNGG 2 cut(s) 853, 974
BmsI GCATC 3 cut(s) 65, 91, 298
BpmI CTGGAG 1 cut(s) 851
BpuEI CTTGAG 2 cut(s) 299, 369
BsaI GGTCTC 1 cut(s) 872
BsaJI CCNNGG 7 cut(s) 165, 592, 640, 852, 921, 1037, 1045
BsaWI WCCGGW 1 cut(s) 799
Bsc4I CCNNNNNNNGG 1 cut(s) 641
Bse118I RCCGGY 2 cut(s) 104, 959
Bse1I ACTGG 3 cut(s) 75, 744, 1012
BseBI CCWGG 2 cut(s) 853, 974
BseDI CCNNGG 7 cut(s) 165, 592, 640, 852, 921, 1037, 1045
BseGI GGATG 4 cut(s) 427, 482, 727, 969
BseLI CCNNNNNNNGG 1 cut(s) 641
BseMII CTCAG 2 cut(s) 767, 893
BseNI ACTGG 3 cut(s) 75, 744, 1012
BseRI GAGGAG 3 cut(s) 296, 629, 1008
BseX3I CGGCCG 2 cut(s) 736, 957
BseXI GCAGC 1 cut(s) 58
BsgI GTGCAG 1 cut(s) 357
Bsh1285I CGRYCG 2 cut(s) 739, 960
BshFI GGCC 6 cut(s) 671, 738, 920, 926, 959, 1011
BsiEI CGRYCG 2 cut(s) 739, 960
BsiHKCI CYCGRG 1 cut(s) 447
BsiSI CCGG 3 cut(s) 105, 800, 960
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 1 cut(s) 641
BsmAI GTCTC 3 cut(s) 278, 667, 872
BsmFI GGGAC 1 cut(s) 97
BsnI GGCC 6 cut(s) 671, 738, 920, 926, 959, 1011
Bso31I GGTCTC 1 cut(s) 872
BsoBI CYCGRG 1 cut(s) 447
Bsp143I GATC 2 cut(s) 153, 1057
BspACI CCGC 8 cut(s) 181, 311, 668, 735, 980, 986, 1050, 1084
BspANI GGCC 6 cut(s) 671, 738, 920, 926, 959, 1011
BspCNI CTCAG 2 cut(s) 766, 894
BspHI TCATGA 1 cut(s) 1054
BspLI GGNNCC 3 cut(s) 834, 978, 1043
BspMI ACCTGC 1 cut(s) 850
BspTI CTTAAG 1 cut(s) 472
BspTNI GGTCTC 1 cut(s) 872
BsrFI RCCGGY 2 cut(s) 104, 959
BsrI ACTGG 3 cut(s) 75, 744, 1012
BssAI RCCGGY 2 cut(s) 104, 959
BssECI CCNNGG 7 cut(s) 165, 592, 640, 852, 921, 1037, 1045
BssMI GATC 2 cut(s) 153, 1057
BssNAI GTATAC 1 cut(s) 40
BssT1I CCWWGG 4 cut(s) 165, 592, 921, 1037
Bst1107I GTATAC 1 cut(s) 40
Bst2UI CCWGG 2 cut(s) 853, 974
Bst4CI ACNGT 3 cut(s) 90, 406, 949
Bst6I CTCTTC 2 cut(s) 224, 309
BstAFI CTTAAG 1 cut(s) 472
BstC8I GCNNGC 2 cut(s) 984, 1070
BstDEI CTNAG 4 cut(s) 520, 614, 753, 902
BstDSI CCRYGG 1 cut(s) 1045
BstF5I GGATG 4 cut(s) 427, 482, 727, 969
BstKTI GATC 2 cut(s) 156, 1060
BstMAI GTCTC 3 cut(s) 278, 667, 872
BstMBI GATC 2 cut(s) 153, 1057
BstMCI CGRYCG 2 cut(s) 739, 960
BstMWI GCNNNNNNNGC 2 cut(s) 617, 749
BstNI CCWGG 2 cut(s) 853, 974
BstNSI RCATGY 1 cut(s) 847
BstSCI CCNGG 2 cut(s) 851, 972
BstV1I GCAGC 1 cut(s) 58
BstZ17I GTATAC 1 cut(s) 40
BstZI CGGCCG 2 cut(s) 736, 957
BsuRI GGCC 6 cut(s) 671, 738, 920, 926, 959, 1011
BtgI CCRYGG 1 cut(s) 1045
BtsCI GGATG 4 cut(s) 427, 482, 727, 969
BtsIMutI CAGTG 1 cut(s) 396
BveI ACCTGC 1 cut(s) 850
Cac8I GCNNGC 2 cut(s) 984, 1070
CciI TCATGA 1 cut(s) 1054
Cfr10I RCCGGY 2 cut(s) 104, 959
Cfr13I GGNCC 1 cut(s) 918
Csp6I GTAC 2 cut(s) 86, 358
CviAII CATG 4 cut(s) 568, 844, 1013, 1055
CviQI GTAC 2 cut(s) 86, 358
DdeI CTNAG 4 cut(s) 520, 614, 753, 902
DpnI GATC 2 cut(s) 155, 1059
DpnII GATC 2 cut(s) 153, 1057
EaeI YGGCCR 3 cut(s) 736, 957, 1009
EagI CGGCCG 2 cut(s) 736, 957
Eam1104I CTCTTC 2 cut(s) 224, 309
EarI CTCTTC 2 cut(s) 224, 309
EclXI CGGCCG 2 cut(s) 736, 957
Eco130I CCWWGG 4 cut(s) 165, 592, 921, 1037
Eco31I GGTCTC 1 cut(s) 872
Eco52I CGGCCG 2 cut(s) 736, 957
Eco57I CTGAAG 1 cut(s) 360
Eco88I CYCGRG 1 cut(s) 447
EcoRII CCWGG 2 cut(s) 851, 972
EcoT14I CCWWGG 4 cut(s) 165, 592, 921, 1037
ErhI CCWWGG 4 cut(s) 165, 592, 921, 1037
FaeI CATG 4 cut(s) 571, 847, 1016, 1058
FalI AAGNNNNNCTT 2 cut(s) 315, 347
FaqI GGGAC 1 cut(s) 97
FatI CATG 4 cut(s) 567, 843, 1012, 1054
FauNDI CATATG 1 cut(s) 134
FblI GTMKAC 1 cut(s) 39
Fnu4HI GCNGC 4 cut(s) 72, 669, 736, 987
FokI GGATG 4 cut(s) 434, 469, 734, 956
Fsp4HI GCNGC 4 cut(s) 72, 669, 736, 987
FspBI CTAG 3 cut(s) 512, 761, 770
GluI GCNGC 4 cut(s) 72, 669, 736, 987
GsuI CTGGAG 1 cut(s) 851
HaeIII GGCC 6 cut(s) 671, 738, 920, 926, 959, 1011
HapII CCGG 3 cut(s) 105, 800, 960
Hin1II CATG 4 cut(s) 571, 847, 1016, 1058
HincII GTYRAC 1 cut(s) 997
HindII GTYRAC 1 cut(s) 997
HindIII AAGCTT 1 cut(s) 545
HinfI GANTC 5 cut(s) 257, 328, 599, 998, 1063
HpaII CCGG 3 cut(s) 105, 800, 960
HphI GGTGA 2 cut(s) 487, 608
Hpy166II GTNNAC 3 cut(s) 40, 534, 997
Hpy188I TCNGA 4 cut(s) 262, 903, 966, 1062
Hpy188III TCNNGA 4 cut(s) 64, 386, 603, 1055
Hpy8I GTNNAC 3 cut(s) 40, 534, 997
Hpy99I CGWCG 1 cut(s) 949
HpyAV CCTTC 2 cut(s) 682, 794
HpyCH4III ACNGT 3 cut(s) 90, 406, 949
HpyCH4V TGCA 6 cut(s) 56, 138, 244, 338, 567, 620
HpyF10VI GCNNNNNNNGC 2 cut(s) 617, 749
HpyF3I CTNAG 4 cut(s) 520, 614, 753, 902
Hsp92II CATG 4 cut(s) 571, 847, 1016, 1058
Kzo9I GATC 2 cut(s) 153, 1057
LmnI GCTCC 5 cut(s) 262, 832, 856, 1021, 1041
Lsp1109I GCAGC 1 cut(s) 58
LweI GCATC 3 cut(s) 65, 91, 298
MaeI CTAG 3 cut(s) 512, 761, 770
MaeIII GTNAC 1 cut(s) 596
MalI GATC 2 cut(s) 155, 1059
MboI GATC 2 cut(s) 153, 1057
MboII GAAGA 5 cut(s) 241, 326, 551, 788, 923
MfeI CAATTG 2 cut(s) 621, 990
MlsI TGGCCA 1 cut(s) 1011
MluCI AATT 6 cut(s) 139, 196, 582, 621, 707, 990
MluNI TGGCCA 1 cut(s) 1011
MlyI GAGTC 2 cut(s) 593, 992
MmeI TCCRAC 4 cut(s) 349, 416, 804, 870
Mox20I TGGCCA 1 cut(s) 1011
MscI TGGCCA 1 cut(s) 1011
MseI TTAA 2 cut(s) 473, 543
MslI CAYNNNNRTG 1 cut(s) 133
Msp20I TGGCCA 1 cut(s) 1011
MspA1I CMGCKG 2 cut(s) 982, 1086
MspCI CTTAAG 1 cut(s) 472
MspI CCGG 3 cut(s) 105, 800, 960
MspR9I CCNGG 2 cut(s) 853, 974
MunI CAATTG 2 cut(s) 621, 990
MvaI CCWGG 2 cut(s) 853, 974
MwoI GCNNNNNNNGC 2 cut(s) 617, 749
NdeI CATATG 1 cut(s) 134
NdeII GATC 2 cut(s) 153, 1057
NlaIII CATG 4 cut(s) 571, 847, 1016, 1058
NlaIV GGNNCC 3 cut(s) 834, 978, 1043
NmeAIII GCCGAG 2 cut(s) 621, 644
NmuCI GTSAC 1 cut(s) 596
NspI RCATGY 1 cut(s) 847
PaeR7I CTCGAG 1 cut(s) 447
PagI TCATGA 1 cut(s) 1054
PcsI WCGNNNNNNNCGW 1 cut(s) 870
PfeI GAWTC 3 cut(s) 257, 328, 1063
PkrI GCNGC 4 cut(s) 73, 670, 737, 988
PleI GAGTC 2 cut(s) 593, 992
PpsI GAGTC 2 cut(s) 593, 992
Psp6I CCWGG 2 cut(s) 851, 972
PspGI CCWGG 2 cut(s) 851, 972
PspN4I GGNNCC 3 cut(s) 834, 978, 1043
PspPI GGNCC 1 cut(s) 918
PspXI VCTCGAGB 1 cut(s) 447
RsaI GTAC 2 cut(s) 87, 359
RsaNI GTAC 2 cut(s) 86, 358
RseI CAYNNNNRTG 1 cut(s) 133
SaqAI TTAA 2 cut(s) 473, 543
SatI GCNGC 4 cut(s) 72, 669, 736, 987
Sau3AI GATC 2 cut(s) 153, 1057
Sau96I GGNCC 1 cut(s) 918
SchI GAGTC 2 cut(s) 593, 992
ScrFI CCNGG 2 cut(s) 853, 974
SfaNI GCATC 3 cut(s) 65, 91, 298
Sfr274I CTCGAG 1 cut(s) 447
SlaI CTCGAG 1 cut(s) 447
SmiMI CAYNNNNRTG 1 cut(s) 133
SmlI CTYRAG 4 cut(s) 278, 384, 447, 472
SmoI CTYRAG 4 cut(s) 278, 384, 447, 472
Sse9I AATT 6 cut(s) 139, 196, 582, 621, 707, 990
SsiI CCGC 8 cut(s) 181, 311, 668, 735, 980, 986, 1050, 1084
SspMI CTAG 3 cut(s) 512, 761, 770
StyD4I CCNGG 2 cut(s) 851, 972
StyI CCWWGG 4 cut(s) 165, 592, 921, 1037
TaaI ACNGT 3 cut(s) 90, 406, 949
TaqI TCGA 2 cut(s) 448, 461
TaqII GACCGA 1 cut(s) 854
TasI AATT 6 cut(s) 139, 196, 582, 621, 707, 990
TatI WGTACW 1 cut(s) 85
TauI GCSGC 3 cut(s) 671, 738, 989
TfiI GAWTC 3 cut(s) 257, 328, 1063
Tru1I TTAA 2 cut(s) 473, 543
Tru9I TTAA 2 cut(s) 473, 543
TscAI CASTG 1 cut(s) 403
TseFI GTSAC 1 cut(s) 596
TseI GCWGC 1 cut(s) 71
Tsp45I GTSAC 1 cut(s) 596
TspDTI ATGAA 5 cut(s) 17, 438, 471, 741, 806
TspGWI ACGGA 1 cut(s) 306
TspRI CASTG 1 cut(s) 403
Vha464I CTTAAG 1 cut(s) 472
XceI RCATGY 1 cut(s) 847
XhoI CTCGAG 1 cut(s) 447
XmiI GTMKAC 1 cut(s) 39
XspI CTAG 3 cut(s) 512, 761, 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.