Rorug06G0169400

Thiamine-repressible mitochondrial transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005884
Physical Location & Seq
Reverse (-)
25035633 .. 25036390
758 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0169400.1

Sequence Viewer

Length: 438 bp
ATGTCTAAAGCACCAGCTCAAGAAGAAGCAGTGCATGCTTATGGATGGGCTGCTAGAAATTCCTCAGGAATTCTCTCCCCCTACCATTTCACTAGAAGGGCTAATGGCGATAATGATATCACCATCAAGGTACAATATTGTGGAATTTGCCACGGAGACATTCACCTCGTAAAGAACAAAATATGGCCGACCATTTATCCAACCGTGCCCGGGCATGAAATTGTTGGCCAAGTGACCAAGGTTGGGCTCAACGTAAAAAAATTTAAAGTTGGAGATGTAGCCCGAGTAGGGTGCATTATGGGTTCATATGGCTCATGCGAGAACTGCAAACAAGACTTGGAAAATTATTGCCCCAAAATGTTATGGACCTTCGATGGACAATATGAAGATGGAACAAGAGCCTTTGGTGGATATTCAGATAAGTTTGTTGTGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

145

Amino Acids

16.1

Weight (kDa)

8.5

Isoelectric Point (pI)

34.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 36 - 144 1.4e-21 Alcohol dehydrogenase GroES-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 185, 226
AcsI RAATTY 4 cut(s) 58, 69, 144, 260
AfaI GTAC 1 cut(s) 132
AfiI CCNNNNNNNGG 3 cut(s) 210, 243, 288
AluBI AGCT 1 cut(s) 17
AluI AGCT 1 cut(s) 17
Alw26I GTCTC 1 cut(s) 150
Ama87I CYCGRG 2 cut(s) 209, 282
AoxI GGCC 2 cut(s) 185, 226
ApeKI GCWGC 1 cut(s) 50
ApoI RAATTY 4 cut(s) 58, 69, 144, 260
AspS9I GGNCC 1 cut(s) 366
AsuC2I CCSGG 2 cut(s) 210, 211
AsuHPI GGTGA 2 cut(s) 112, 155
AvaI CYCGRG 2 cut(s) 209, 282
AvaII GGWCC 1 cut(s) 366
AxyI CCTNAGG 1 cut(s) 64
BaeGI GKGCMC 1 cut(s) 210
BalI TGGCCA 1 cut(s) 228
BanII GRGCYC 1 cut(s) 249
BbvI GCAGC 1 cut(s) 37
BccI CCATC 4 cut(s) 39, 131, 368, 383
BcgI CGANNNNNNTGC 2 cut(s) 273, 307
BcnI CCSGG 2 cut(s) 210, 211
BcoDI GTCTC 1 cut(s) 150
BfaI CTAG 2 cut(s) 54, 93
BisI GCNGC 1 cut(s) 51
BlsI GCNGC 1 cut(s) 52
Bme1390I CCNGG 2 cut(s) 210, 211
Bme18I GGWCC 1 cut(s) 366
BmeT110I CYCGRG 2 cut(s) 209, 282
BmgT120I GGNCC 1 cut(s) 366
BmrFI CCNGG 2 cut(s) 210, 211
BpuMI CCSGG 2 cut(s) 210, 211
BsaJI CCNNGG 3 cut(s) 151, 209, 237
Bsc4I CCNNNNNNNGG 3 cut(s) 210, 243, 288
Bse21I CCTNAGG 1 cut(s) 64
BseDI CCNNGG 3 cut(s) 151, 209, 237
BseGI GGATG 1 cut(s) 50
BseLI CCNNNNNNNGG 3 cut(s) 210, 243, 288
BseMII CTCAG 1 cut(s) 78
BseSI GKGCMC 1 cut(s) 210
BseXI GCAGC 1 cut(s) 37
BshFI GGCC 2 cut(s) 187, 228
BsiHKCI CYCGRG 2 cut(s) 209, 282
BsiSI CCGG 1 cut(s) 210
BslI CCNNNNNNNGG 3 cut(s) 210, 243, 288
BsmAI GTCTC 1 cut(s) 150
BsnI GGCC 2 cut(s) 187, 228
BsoBI CYCGRG 2 cut(s) 209, 282
Bsp1286I GDGCHC 2 cut(s) 210, 249
BspANI GGCC 2 cut(s) 187, 228
BspCNI CTCAG 1 cut(s) 77
BssECI CCNNGG 3 cut(s) 151, 209, 237
BssT1I CCWWGG 1 cut(s) 237
Bst4CI ACNGT 1 cut(s) 205
BstAPI GCANNNNNTGC 1 cut(s) 35
BstC8I GCNNGC 1 cut(s) 36
BstDEI CTNAG 1 cut(s) 64
BstDSI CCRYGG 1 cut(s) 151
BstF5I GGATG 1 cut(s) 50
BstMAI GTCTC 1 cut(s) 150
BstMWI GCNNNNNNNGC 2 cut(s) 35, 324
BstNSI RCATGY 1 cut(s) 38
BstSCI CCNGG 2 cut(s) 208, 209
BstSLI GKGCMC 1 cut(s) 210
BstV1I GCAGC 1 cut(s) 37
Bsu36I CCTNAGG 1 cut(s) 64
BsuRI GGCC 2 cut(s) 187, 228
BtgI CCRYGG 1 cut(s) 151
BtsCI GGATG 1 cut(s) 50
BtsI GCAGTG 1 cut(s) 36
BtsIMutI CAGTG 1 cut(s) 36
Cac8I GCNNGC 1 cut(s) 36
Cfr13I GGNCC 1 cut(s) 366
Cfr9I CCCGGG 1 cut(s) 209
Csp6I GTAC 1 cut(s) 131
CviAII CATG 3 cut(s) 35, 215, 315
CviJI RGCY 9 cut(s) 17, 50, 101, 187, 228, 247, 281, 312, 401
CviKI_1 RGCY 9 cut(s) 17, 50, 101, 187, 228, 247, 281, 312, 401
CviQI GTAC 1 cut(s) 131
DdeI CTNAG 1 cut(s) 64
DraI TTTAAA 1 cut(s) 265
EaeI YGGCCR 2 cut(s) 185, 226
Eco130I CCWWGG 1 cut(s) 237
Eco24I GRGCYC 1 cut(s) 249
Eco32I GATATC 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 366
Eco81I CCTNAGG 1 cut(s) 64
Eco88I CYCGRG 2 cut(s) 209, 282
EcoRI GAATTC 1 cut(s) 69
EcoRV GATATC 1 cut(s) 118
EcoT14I CCWWGG 1 cut(s) 237
EcoT38I GRGCYC 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 237
FaeI CATG 3 cut(s) 38, 218, 318
FatI CATG 3 cut(s) 34, 214, 314
FauNDI CATATG 1 cut(s) 307
Fnu4HI GCNGC 1 cut(s) 51
FokI GGATG 1 cut(s) 57
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 1 cut(s) 51
FspBI CTAG 2 cut(s) 54, 93
GluI GCNGC 1 cut(s) 51
HaeIII GGCC 2 cut(s) 187, 228
HapII CCGG 1 cut(s) 210
Hin1II CATG 3 cut(s) 38, 218, 318
HpaII CCGG 1 cut(s) 210
HphI GGTGA 2 cut(s) 112, 155
Hpy188I TCNGA 1 cut(s) 418
Hpy188III TCNNGA 2 cut(s) 20, 66
HpyAV CCTTC 2 cut(s) 90, 379
HpyCH4III ACNGT 1 cut(s) 205
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 3 cut(s) 34, 294, 327
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 324
HpyF3I CTNAG 1 cut(s) 64
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 3 cut(s) 38, 218, 318
LpnPI CCDG 3 cut(s) 27, 51, 223
Lsp1109I GCAGC 1 cut(s) 37
MaeI CTAG 2 cut(s) 54, 93
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 1 cut(s) 232
MboII GAAGA 2 cut(s) 35, 398
MhlI GDGCHC 2 cut(s) 210, 249
MlsI TGGCCA 1 cut(s) 228
MluCI AATT 6 cut(s) 58, 69, 144, 219, 260, 343
MluNI TGGCCA 1 cut(s) 228
MmeI TCCRAC 2 cut(s) 224, 250
MnlI CCTC 2 cut(s) 73, 176
Mox20I TGGCCA 1 cut(s) 228
MscI TGGCCA 1 cut(s) 228
MseI TTAA 1 cut(s) 264
MslI CAYNNNNRTG 1 cut(s) 39
Msp20I TGGCCA 1 cut(s) 228
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 2 cut(s) 210, 211
MwoI GCNNNNNNNGC 2 cut(s) 35, 324
NciI CCSGG 2 cut(s) 210, 211
NdeI CATATG 1 cut(s) 307
NlaIII CATG 3 cut(s) 38, 218, 318
NmuCI GTSAC 1 cut(s) 232
NspI RCATGY 1 cut(s) 38
PaeI GCATGC 1 cut(s) 38
PkrI GCNGC 1 cut(s) 52
PspPI GGNCC 1 cut(s) 366
RsaI GTAC 1 cut(s) 132
RsaNI GTAC 1 cut(s) 131
RseI CAYNNNNRTG 1 cut(s) 39
SaqAI TTAA 1 cut(s) 264
SatI GCNGC 1 cut(s) 51
Sau96I GGNCC 1 cut(s) 366
ScrFI CCNGG 2 cut(s) 210, 211
SduI GDGCHC 2 cut(s) 210, 249
SetI ASST 6 cut(s) 19, 132, 168, 243, 255, 371
SinI GGWCC 1 cut(s) 366
SmaI CCCGGG 1 cut(s) 211
SmiMI CAYNNNNRTG 1 cut(s) 39
SmlI CTYRAG 1 cut(s) 18
SmoI CTYRAG 1 cut(s) 18
SphI GCATGC 1 cut(s) 38
SrfI GCCCGGGC 1 cut(s) 211
Sse9I AATT 6 cut(s) 58, 69, 144, 219, 260, 343
SspI AATATT 1 cut(s) 137
SspMI CTAG 2 cut(s) 54, 93
StyD4I CCNGG 2 cut(s) 208, 209
StyI CCWWGG 1 cut(s) 237
TaaI ACNGT 1 cut(s) 205
TaiI ACGT 1 cut(s) 255
TaqI TCGA 1 cut(s) 372
TasI AATT 6 cut(s) 58, 69, 144, 219, 260, 343
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TscAI CASTG 1 cut(s) 36
TseFI GTSAC 1 cut(s) 232
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 1 cut(s) 232
TspDTI ATGAA 3 cut(s) 231, 294, 399
TspGWI ACGGA 1 cut(s) 168
TspMI CCCGGG 1 cut(s) 209
TspRI CASTG 1 cut(s) 36
VpaK11BI GGWCC 1 cut(s) 366
XapI RAATTY 4 cut(s) 58, 69, 144, 260
XceI RCATGY 1 cut(s) 38
XmaI CCCGGG 1 cut(s) 209
XspI CTAG 2 cut(s) 54, 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.