Rroxscaffold_7G00184160

Thiamine-repressible mitochondrial transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005884
Physical Location & Seq
Forward (+)
23359183 .. 23362995
3813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00184160.1

Sequence Viewer

Length: 1302 bp
ATGAAAAGTGAAGGTTTGAAGTGGGTTTTAGGTTTGATATACTTATTTGCTGTTGCATCTATTTGGATAGCTGCTAGTTTTGTTGTACAGTCGGTTGTGGATGCCGGTGTGTCCCCTTTTCTTATTACCTACATATGCAATTCATTGTTTGTGATCTACATTCCCTTGGTTGAAGCCGGGCGATATTTGGAGGATTCGTTAGGAGGGTTTTGGTTTTGGAAAAGTAAGGAGAGTAGTCCTTTGCGAGAGTTGGTGGAGTCGGAGCAATCCACTCTTCTTGGAGACGGTGATGTTGTTGTGAAAACTGATGATGTAGAAGAGGGAGAAATTGGCGGACATGGAAAAGTTATTGATGGAGAAGTGAAAGTTGTGGCGTATGAGCCTATTAGGATATCAGCGAATCTGTCTGAAATCGATGAAGTTGCTGATAAGCAAGTGGATGAAAAAGGGCGTTGGACACGTACTCGAGTGGCAAAAGTTAGCCTATTGATTTCCCCATTTTGGTTTTTCGCACAGCTCACTTTCAATTTGTCATTGAAGTATACTACAGTCACATCAAATACCATCTTAAGCAGTGCATCCAGCCTGTTCACCTTTTTGGTCTCTCTAGCATTCTTAGGTGAGAACTTCACTTTGGTGAAGCTTTTCAGTGTTCTTCTTTGCATGGGAGGAACGATAATTGTCAGCCTCGGTGACTCACGAACTACTCTAAGTGCAATTGCTTCAAACCCTCTCCTGGGAGACATACTTGCTCTTGTCTCAGCAGCTTTGTATTCTGTGTATATTACCCTCATTCGCAAGAAGTTACCTGATGAGGATGATGAGAAAAGTGGTCGTGCTAGTATGGCTCAGTTTCTGGGATTTTTAGGGCTTTCTAACCTTCTTATATTTCTTCCAGTTGCCCTTGTACTGCATTTCTCAAAGTTGGAACCCTTTTATATGCTGACCTGGAAGCAGCTCGGTCTCATTGTTGGTAAAGGTTTGCTGGATAACGTTCTGAGCGATTACTTATGGGCCAAGGCTGTTCTTCTAACAACAACAACAGTAGCAACAGCTGGTCTATCAATACAAGTTCCGTTGGCAGCAATTGTAGACTCTGTGACTGGCCATGCTCCTCATTTTGCTGATTACCTTGGAGCTGTGGCCGTTATGATTGGATTTGTTGGCATTAATATTCCTTCTGATGCCTTTAAGAGGTCGAAAGGAGCTACACTAGAATTAGAGAATGGAAATAATAGAAATAGTAGTTCAATTAGTGAACCTGGTAGTTCATCAGGTCCAGATTCAACTGCCCATTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

433

Amino Acids

46.73

Weight (kDa)

5.04

Isoelectric Point (pI)

30.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 143 - 267 5.1e-07 Purine nucleobase transmembrane transport
EamA PF00892 160 - 229 4.8e-09 EamA-like transporter family
TPT PF03151 165 - 312 2.9e-06 Triose-phosphate Transporter family
SLC35F PF06027 179 - 263 6.8e-08 Solute carrier family 35
EamA PF00892 246 - 391 6.4e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 542, 1092
AciI CCGC 1 cut(s) 333
AclI AACGTT 1 cut(s) 993
AcoI YGGCCR 2 cut(s) 1105, 1143
AfaI GTAC 3 cut(s) 87, 463, 909
AfiI CCNNNNNNNGG 4 cut(s) 501, 736, 737, 1194
AflII CTTAAG 1 cut(s) 568
AflIII ACRYGT 1 cut(s) 458
AgsI TTSAA 7 cut(s) 19, 173, 526, 538, 726, 1251, 1287
AjnI CCWGG 3 cut(s) 735, 947, 1261
AjuI GAANNNNNNNTTGG 2 cut(s) 617, 649
AleI CACNNNNGTG 1 cut(s) 635
AluBI AGCT 8 cut(s) 71, 517, 643, 767, 958, 1055, 1139, 1208
AluI AGCT 8 cut(s) 71, 517, 643, 767, 958, 1055, 1139, 1208
Alw26I GTCTC 5 cut(s) 276, 607, 735, 763, 968
AlwNI CAGNNNCTG 1 cut(s) 856
Ama87I CYCGRG 1 cut(s) 465
AoxI GGCC 3 cut(s) 1014, 1105, 1143
ApeKI GCWGC 4 cut(s) 71, 764, 955, 1082
AseI ATTAAT 1 cut(s) 1170
Asp700I GAANNNNTTC 1 cut(s) 644
AspS9I GGNCC 2 cut(s) 1014, 1277
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 5 cut(s) 299, 583, 632, 649, 704
AvaI CYCGRG 1 cut(s) 465
AvaII GGWCC 1 cut(s) 1277
BalI TGGCCA 1 cut(s) 1107
BbvI GCAGC 4 cut(s) 58, 776, 967, 1094
BccI CCATC 2 cut(s) 347, 572
BceAI ACGGC 1 cut(s) 1130
BcgI CGANNNNNNTGC 2 cut(s) 404, 438
BciT130I CCWGG 3 cut(s) 737, 949, 1263
BcnI CCSGG 1 cut(s) 178
BcoDI GTCTC 5 cut(s) 276, 607, 735, 763, 968
BfaI CTAG 4 cut(s) 75, 608, 840, 1214
BfmI CTRYAG 1 cut(s) 546
BfrI CTTAAG 1 cut(s) 568
BisI GCNGC 4 cut(s) 72, 765, 956, 1083
BlsI GCNGC 4 cut(s) 73, 766, 957, 1084
Bme1390I CCNGG 4 cut(s) 178, 737, 949, 1263
Bme18I GGWCC 1 cut(s) 1277
BmeT110I CYCGRG 1 cut(s) 465
BmgT120I GGNCC 2 cut(s) 1014, 1277
BmiI GGNNCC 1 cut(s) 930
BmrFI CCNGG 4 cut(s) 178, 737, 949, 1263
BmsI GCATC 4 cut(s) 65, 91, 587, 1174
BpuMI CCSGG 1 cut(s) 178
Bsa29I ATCGAT 1 cut(s) 414
BsaAI YACGTR 1 cut(s) 461
BsaI GGTCTC 2 cut(s) 607, 968
BsaJI CCNNGG 5 cut(s) 165, 688, 736, 1017, 1132
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 4 cut(s) 501, 736, 737, 1194
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 896, 1108
BseBI CCWGG 3 cut(s) 737, 949, 1263
BseCI ATCGAT 1 cut(s) 414
BseDI CCNNGG 5 cut(s) 165, 688, 736, 1017, 1132
BseGI GGATG 4 cut(s) 106, 445, 578, 823
BseLI CCNNNNNNNGG 4 cut(s) 501, 736, 737, 1194
BseMII CTCAG 3 cut(s) 774, 863, 989
BseNI ACTGG 2 cut(s) 896, 1108
BseRI GAGGAG 1 cut(s) 1104
BseXI GCAGC 4 cut(s) 58, 776, 967, 1094
BshFI GGCC 3 cut(s) 1016, 1107, 1145
BshVI ATCGAT 1 cut(s) 414
BsiHKCI CYCGRG 1 cut(s) 465
BsiSI CCGG 2 cut(s) 105, 177
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 4 cut(s) 501, 736, 737, 1194
BsmAI GTCTC 5 cut(s) 276, 607, 735, 763, 968
BsmBI CGTCTC 1 cut(s) 276
BsmFI GGGAC 1 cut(s) 97
BsmI GAATGC 1 cut(s) 611
BsnI GGCC 3 cut(s) 1016, 1107, 1145
Bso31I GGTCTC 2 cut(s) 607, 968
BsoBI CYCGRG 1 cut(s) 465
Bsp1407I TGTACA 1 cut(s) 85
Bsp143I GATC 1 cut(s) 153
BspACI CCGC 1 cut(s) 333
BspANI GGCC 3 cut(s) 1016, 1107, 1145
BspCNI CTCAG 3 cut(s) 773, 862, 990
BspDI ATCGAT 1 cut(s) 414
BspLI GGNNCC 1 cut(s) 930
BspTI CTTAAG 1 cut(s) 568
BspTNI GGTCTC 2 cut(s) 607, 968
BsrFI RCCGGY 1 cut(s) 104
BsrGI TGTACA 1 cut(s) 85
BsrI ACTGG 2 cut(s) 896, 1108
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 5 cut(s) 165, 688, 736, 1017, 1132
BssMI GATC 1 cut(s) 153
BssNAI GTATAC 1 cut(s) 543
BssT1I CCWWGG 3 cut(s) 165, 1017, 1132
Bst1107I GTATAC 1 cut(s) 543
Bst2UI CCWGG 3 cut(s) 737, 949, 1263
Bst4CI ACNGT 4 cut(s) 90, 287, 550, 1045
Bst6I CTCTTC 2 cut(s) 279, 312
BstAFI CTTAAG 1 cut(s) 568
BstAUI TGTACA 1 cut(s) 85
BstBAI YACGTR 1 cut(s) 461
BstDEI CTNAG 5 cut(s) 616, 710, 760, 849, 998
BstENI CCTNNNNNAGG 1 cut(s) 1192
BstF5I GGATG 4 cut(s) 106, 445, 578, 823
BstKTI GATC 1 cut(s) 156
BstMAI GTCTC 5 cut(s) 276, 607, 735, 763, 968
BstMBI GATC 1 cut(s) 153
BstMWI GCNNNNNNNGC 1 cut(s) 845
BstNI CCWGG 3 cut(s) 737, 949, 1263
BstSCI CCNGG 4 cut(s) 176, 735, 947, 1261
BstSFI CTRYAG 1 cut(s) 546
BstV1I GCAGC 4 cut(s) 58, 776, 967, 1094
BstZ17I GTATAC 1 cut(s) 543
Bsu15I ATCGAT 1 cut(s) 414
BsuRI GGCC 3 cut(s) 1016, 1107, 1145
BsuTUI ATCGAT 1 cut(s) 414
BtsCI GGATG 4 cut(s) 106, 445, 578, 823
BtsI GCAGTG 1 cut(s) 580
BtsIMutI CAGTG 2 cut(s) 580, 655
CaiI CAGNNNCTG 1 cut(s) 856
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 2 cut(s) 1014, 1277
ClaI ATCGAT 1 cut(s) 414
CsiI ACCWGGT 1 cut(s) 1261
Csp6I GTAC 3 cut(s) 86, 462, 908
CspCI CAANNNNNGTGG 2 cut(s) 259, 294
CviAII CATG 3 cut(s) 338, 664, 1109
CviQI GTAC 3 cut(s) 86, 462, 908
DdeI CTNAG 5 cut(s) 616, 710, 760, 849, 998
DpnI GATC 1 cut(s) 155
DpnII GATC 1 cut(s) 153
EaeI YGGCCR 2 cut(s) 1105, 1143
Eam1104I CTCTTC 2 cut(s) 279, 312
EarI CTCTTC 2 cut(s) 279, 312
EciI GGCGGA 1 cut(s) 348
Eco130I CCWWGG 3 cut(s) 165, 1017, 1132
Eco31I GGTCTC 2 cut(s) 607, 968
Eco32I GATATC 1 cut(s) 393
Eco47I GGWCC 1 cut(s) 1277
Eco88I CYCGRG 1 cut(s) 465
EcoNI CCTNNNNNAGG 1 cut(s) 1192
EcoRII CCWGG 3 cut(s) 735, 947, 1261
EcoRV GATATC 1 cut(s) 393
EcoT14I CCWWGG 3 cut(s) 165, 1017, 1132
ErhI CCWWGG 3 cut(s) 165, 1017, 1132
Esp3I CGTCTC 1 cut(s) 276
FaeI CATG 3 cut(s) 341, 667, 1112
FaqI GGGAC 1 cut(s) 97
FatI CATG 3 cut(s) 337, 663, 1108
FauNDI CATATG 1 cut(s) 134
FblI GTMKAC 2 cut(s) 542, 1092
Fnu4HI GCNGC 4 cut(s) 72, 765, 956, 1083
FokI GGATG 4 cut(s) 113, 452, 565, 830
Fsp4HI GCNGC 4 cut(s) 72, 765, 956, 1083
FspBI CTAG 4 cut(s) 75, 608, 840, 1214
GluI GCNGC 4 cut(s) 72, 765, 956, 1083
HaeIII GGCC 3 cut(s) 1016, 1107, 1145
HapII CCGG 2 cut(s) 105, 177
Hin1II CATG 3 cut(s) 341, 667, 1112
HindIII AAGCTT 1 cut(s) 641
HinfI GANTC 6 cut(s) 194, 257, 400, 695, 1094, 1283
HpaII CCGG 2 cut(s) 105, 177
HphI GGTGA 5 cut(s) 299, 583, 632, 649, 704
Hpy166II GTNNAC 4 cut(s) 543, 591, 1093, 1259
Hpy188I TCNGA 4 cut(s) 262, 409, 999, 1183
Hpy188III TCNNGA 2 cut(s) 699, 1280
Hpy8I GTNNAC 4 cut(s) 543, 591, 1093, 1259
HpyAV CCTTC 3 cut(s) 5, 890, 1188
HpyCH4III ACNGT 4 cut(s) 90, 287, 550, 1045
HpyCH4IV ACGT 2 cut(s) 460, 993
HpyCH4V TGCA 6 cut(s) 56, 138, 578, 663, 716, 913
HpyF10VI GCNNNNNNNGC 1 cut(s) 845
HpyF3I CTNAG 5 cut(s) 616, 710, 760, 849, 998
HpySE526I ACGT 2 cut(s) 460, 993
Hsp92II CATG 3 cut(s) 341, 667, 1112
Kzo9I GATC 1 cut(s) 153
LmnI GCTCC 4 cut(s) 262, 1117, 1136, 1205
Lsp1109I GCAGC 4 cut(s) 58, 776, 967, 1094
LweI GCATC 4 cut(s) 65, 91, 587, 1174
MabI ACCWGGT 1 cut(s) 1261
MaeI CTAG 4 cut(s) 75, 608, 840, 1214
MaeII ACGT 2 cut(s) 460, 993
MaeIII GTNAC 4 cut(s) 550, 692, 804, 1099
MalI GATC 1 cut(s) 155
MboI GATC 1 cut(s) 153
MboII GAAGA 5 cut(s) 266, 329, 647, 884, 1019
MfeI CAATTG 2 cut(s) 717, 1086
MlsI TGGCCA 1 cut(s) 1107
MluCI AATT 8 cut(s) 139, 327, 526, 678, 717, 1086, 1217, 1251
MluNI TGGCCA 1 cut(s) 1107
MlyI GAGTC 3 cut(s) 266, 689, 1088
MmeI TCCRAC 3 cut(s) 240, 434, 906
Mox20I TGGCCA 1 cut(s) 1107
MroXI GAANNNNTTC 1 cut(s) 644
MscI TGGCCA 1 cut(s) 1107
MseI TTAA 3 cut(s) 569, 1170, 1191
MslI CAYNNNNRTG 1 cut(s) 635
Msp20I TGGCCA 1 cut(s) 1107
MspA1I CMGCKG 1 cut(s) 1055
MspCI CTTAAG 1 cut(s) 568
MspI CCGG 2 cut(s) 105, 177
MspR9I CCNGG 4 cut(s) 178, 737, 949, 1263
MunI CAATTG 2 cut(s) 717, 1086
Mva1269I GAATGC 1 cut(s) 611
MvaI CCWGG 3 cut(s) 737, 949, 1263
MwoI GCNNNNNNNGC 1 cut(s) 845
NciI CCSGG 1 cut(s) 178
NdeI CATATG 1 cut(s) 134
NdeII GATC 1 cut(s) 153
NlaIII CATG 3 cut(s) 341, 667, 1112
NlaIV GGNNCC 1 cut(s) 930
NmuCI GTSAC 3 cut(s) 550, 692, 1099
OliI CACNNNNGTG 1 cut(s) 635
PaeR7I CTCGAG 1 cut(s) 465
PcsI WCGNNNNNNNCGW 1 cut(s) 999
PctI GAATGC 1 cut(s) 611
PdmI GAANNNNTTC 1 cut(s) 644
PfeI GAWTC 3 cut(s) 194, 400, 1283
PkrI GCNGC 4 cut(s) 73, 766, 957, 1084
PleI GAGTC 3 cut(s) 265, 689, 1088
PpsI GAGTC 3 cut(s) 265, 689, 1088
Ppu21I YACGTR 1 cut(s) 461
PshBI ATTAAT 1 cut(s) 1170
Psp1406I AACGTT 1 cut(s) 993
Psp6I CCWGG 3 cut(s) 735, 947, 1261
PspGI CCWGG 3 cut(s) 735, 947, 1261
PspN4I GGNNCC 1 cut(s) 930
PspPI GGNCC 2 cut(s) 1014, 1277
PspXI VCTCGAGB 1 cut(s) 465
PstNI CAGNNNCTG 1 cut(s) 856
PvuII CAGCTG 1 cut(s) 1055
RsaI GTAC 3 cut(s) 87, 463, 909
RsaNI GTAC 3 cut(s) 86, 462, 908
RseI CAYNNNNRTG 1 cut(s) 635
SaqAI TTAA 3 cut(s) 569, 1170, 1191
SatI GCNGC 4 cut(s) 72, 765, 956, 1083
Sau3AI GATC 1 cut(s) 153
Sau96I GGNCC 2 cut(s) 1014, 1277
SchI GAGTC 3 cut(s) 266, 689, 1088
ScrFI CCNGG 4 cut(s) 178, 737, 949, 1263
SexAI ACCWGGT 1 cut(s) 1261
SfaNI GCATC 4 cut(s) 65, 91, 587, 1174
SfcI CTRYAG 1 cut(s) 546
Sfr274I CTCGAG 1 cut(s) 465
SinI GGWCC 1 cut(s) 1277
SlaI CTCGAG 1 cut(s) 465
SmiMI CAYNNNNRTG 1 cut(s) 635
SmlI CTYRAG 2 cut(s) 465, 568
SmoI CTYRAG 2 cut(s) 465, 568
Sse9I AATT 8 cut(s) 139, 327, 526, 678, 717, 1086, 1217, 1251
SsiI CCGC 1 cut(s) 333
SspI AATATT 1 cut(s) 1174
SspMI CTAG 4 cut(s) 75, 608, 840, 1214
StyD4I CCNGG 4 cut(s) 176, 735, 947, 1261
StyI CCWWGG 3 cut(s) 165, 1017, 1132
TaaI ACNGT 4 cut(s) 90, 287, 550, 1045
TaiI ACGT 2 cut(s) 463, 996
TaqI TCGA 3 cut(s) 414, 466, 1199
TaqII GACCGA 1 cut(s) 950
TasI AATT 8 cut(s) 139, 327, 526, 678, 717, 1086, 1217, 1251
TatI WGTACW 2 cut(s) 85, 907
TfiI GAWTC 3 cut(s) 194, 400, 1283
Tru1I TTAA 3 cut(s) 569, 1170, 1191
Tru9I TTAA 3 cut(s) 569, 1170, 1191
TscAI CASTG 2 cut(s) 580, 655
TseFI GTSAC 3 cut(s) 550, 692, 1099
TseI GCWGC 4 cut(s) 71, 764, 955, 1082
Tsp45I GTSAC 3 cut(s) 550, 692, 1099
TspDTI ATGAA 6 cut(s) 17, 132, 432, 456, 1260, 1287
TspGWI ACGGA 1 cut(s) 1065
TspRI CASTG 2 cut(s) 580, 655
Vha464I CTTAAG 1 cut(s) 568
VpaK11BI GGWCC 1 cut(s) 1277
VspI ATTAAT 1 cut(s) 1170
XagI CCTNNNNNAGG 1 cut(s) 1192
XhoI CTCGAG 1 cut(s) 465
XmiI GTMKAC 2 cut(s) 542, 1092
XmnI GAANNNNTTC 1 cut(s) 644
XspI CTAG 4 cut(s) 75, 608, 840, 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.