RLG00000012694

Thiamine-repressible mitochondrial transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0005884
Physical Location & Seq
Forward (+)
21398061 .. 21401400
3340 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012694

Sequence Viewer

Length: 1314 bp
ATGAAAAGTGAAGGTTTGAAGTGGGTTTTAGGTTTGATATACTTATTTGCTGTTGCATCTATTTGGATAGCTGCTAGTTTTGTTGTACAGTCGGTGGTGGATGCCGGTGTGTCCCCTTTTCTTATTACCTACATATGCAATTCATTGTTTGTGATCTACATTCCCTTGGTTGAAGCGGGGCGCTATTTGGAGGATTCGTTCGGAGGGTTTTGGTTTTGGAAAAGTAAGGAGAGTAGTCCGTTGCGAGAGTTGGTGGAGTCGGAGCAATCCACTCTTCTTGGAGACGGTGATGCTGTTGCGAAAACTGATGATGCATCTGTGCATGTAGAAGAGGGAGAAATAGGCGGGCATGGAAAAGTTATTGATGGAGAAGTGAAAGTTGTGGCGTATGAGCCTATTAGGATATCAGCGAATCTGTCTGAAATCGATGAAGTTGCTGATAAGCAAGTGGATGAAAAAGGGCGTTGGACACGTACTCGAGTGGCAAAAGTTAGCCTATTGATTTCCCCATTTTGGTTTTTCGCACAGCTCACTTTCAATTTGTCATTGAAGTATACTACAGTCACATCAAATACCATCTTAAGCAGTGCATCCAGCCTGTTCACCTTTTTGGTCTCTCTTGCATTCTTAGGTGAGAACTTCACTTTGGTGAAGCTTTTCAGTGTTCTTCTTTGCATGGGAGGAACGATAATTGTCAGCCTCGGTGACTCACGAACTACTCTAAGTGCAATTGCTTCAAACCCTCTACTGGGAGACATACTTGCTCTTGTCTCAGCAGCTTTGTACTCTGTGTATATTACCCTCATTCGCAAGAAGTTACCTGATGAGGATGATGAGAAAAGTGGTCGTGCTAGTATGGCTCAGTTTCTCGGATTTTTAGGGCTTTCTAACCTTTTAATATTTCTTCCAGTTGCCCTTGTACTGCATTTCTCAAAGTTGGAACCCTTTTATATGCTGACCTGGAACCAGCTCGGTCTCATTGTTGGTAAAGGTTTGCTGGATAACGTTCTGAGCGATTACTTATGGGCCAAGGCTGTTCTTCTAACAACAACCACAGTAGCAACAGCTGGTCTATCAATACAAGTTCCGTTAGCAGCAATTGTAGACTCCGTGACTGGCCATGCTCCTCATTTTGCTGATTACTTTGGAGCTGTGGCCGTTATGATTGGATTTGTTGGCATTAATATTCCTTCTGATGCTTTTAGGAGGTCGAAAGGAGCTACACTAGAATTAGAGAATGGAAATAATAGAAATAGTAGTTCAATTAGTGAACCTGGTAGTTCATCAGGTCCAGATTCAACTGCCCATTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

438

Amino Acids

47.15

Weight (kDa)

5.03

Isoelectric Point (pI)

30.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 147 - 271 5.3e-07 Purine nucleobase transmembrane transport
EamA PF00892 164 - 233 4.9e-09 EamA-like transporter family
TPT PF03151 169 - 315 3e-06 Triose-phosphate Transporter family
SLC35F PF06027 183 - 268 8.9e-08 Solute carrier family 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 554, 1104
AciI CCGC 2 cut(s) 176, 345
AclI AACGTT 1 cut(s) 1005
AcoI YGGCCR 2 cut(s) 1117, 1155
AfaI GTAC 4 cut(s) 87, 475, 785, 921
AfiI CCNNNNNNNGG 3 cut(s) 513, 748, 749
AflII CTTAAG 1 cut(s) 580
AflIII ACRYGT 1 cut(s) 470
AgsI TTSAA 7 cut(s) 19, 173, 538, 550, 738, 1263, 1299
AjnI CCWGG 2 cut(s) 959, 1273
AjuI GAANNNNNNNTTGG 2 cut(s) 629, 661
AleI CACNNNNGTG 1 cut(s) 647
AloI GAACNNNNNNTCC 2 cut(s) 182, 214
AluBI AGCT 8 cut(s) 71, 529, 655, 779, 970, 1067, 1151, 1220
AluI AGCT 8 cut(s) 71, 529, 655, 779, 970, 1067, 1151, 1220
Alw26I GTCTC 5 cut(s) 276, 619, 747, 775, 980
Ama87I CYCGRG 1 cut(s) 477
AoxI GGCC 3 cut(s) 1026, 1117, 1155
ApeKI GCWGC 3 cut(s) 71, 776, 1094
AseI ATTAAT 1 cut(s) 1182
Asp700I GAANNNNTTC 1 cut(s) 656
AspLEI GCGC 1 cut(s) 183
AspS9I GGNCC 2 cut(s) 1026, 1289
AsuHPI GGTGA 5 cut(s) 299, 595, 644, 661, 716
AvaI CYCGRG 1 cut(s) 477
AvaII GGWCC 1 cut(s) 1289
BalI TGGCCA 1 cut(s) 1119
BbvI GCAGC 3 cut(s) 58, 788, 1106
BccI CCATC 2 cut(s) 359, 584
BceAI ACGGC 1 cut(s) 1142
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BciT130I CCWGG 2 cut(s) 961, 1275
BcoDI GTCTC 5 cut(s) 276, 619, 747, 775, 980
BfaI CTAG 3 cut(s) 75, 852, 1226
BfmI CTRYAG 1 cut(s) 558
BfoI RGCGCY 1 cut(s) 184
BfrI CTTAAG 1 cut(s) 580
BisI GCNGC 3 cut(s) 72, 777, 1095
BlsI GCNGC 3 cut(s) 73, 778, 1096
Bme1390I CCNGG 2 cut(s) 961, 1275
Bme18I GGWCC 1 cut(s) 1289
BmeT110I CYCGRG 1 cut(s) 477
BmgT120I GGNCC 2 cut(s) 1026, 1289
BmiI GGNNCC 2 cut(s) 942, 965
BmrFI CCNGG 2 cut(s) 961, 1275
BmrI ACTGGG 1 cut(s) 758
BmsI GCATC 7 cut(s) 65, 91, 280, 301, 323, 599, 1186
BmuI ACTGGG 1 cut(s) 758
Bsa29I ATCGAT 1 cut(s) 426
BsaAI YACGTR 1 cut(s) 473
BsaI GGTCTC 2 cut(s) 619, 980
BsaJI CCNNGG 3 cut(s) 165, 700, 1029
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 3 cut(s) 513, 748, 749
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 3 cut(s) 753, 908, 1120
BseBI CCWGG 2 cut(s) 961, 1275
BseCI ATCGAT 1 cut(s) 426
BseDI CCNNGG 3 cut(s) 165, 700, 1029
BseGI GGATG 4 cut(s) 106, 457, 590, 835
BseLI CCNNNNNNNGG 3 cut(s) 513, 748, 749
BseMII CTCAG 3 cut(s) 786, 875, 1001
BseNI ACTGG 3 cut(s) 753, 908, 1120
BseRI GAGGAG 1 cut(s) 1116
BseXI GCAGC 3 cut(s) 58, 788, 1106
BshFI GGCC 3 cut(s) 1028, 1119, 1157
BshVI ATCGAT 1 cut(s) 426
BsiHKCI CYCGRG 1 cut(s) 477
BsiSI CCGG 1 cut(s) 105
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 3 cut(s) 513, 748, 749
BsmAI GTCTC 5 cut(s) 276, 619, 747, 775, 980
BsmBI CGTCTC 1 cut(s) 276
BsmFI GGGAC 1 cut(s) 97
BsmI GAATGC 1 cut(s) 623
BsnI GGCC 3 cut(s) 1028, 1119, 1157
Bso31I GGTCTC 2 cut(s) 619, 980
BsoBI CYCGRG 1 cut(s) 477
Bsp1407I TGTACA 1 cut(s) 85
Bsp143I GATC 1 cut(s) 153
BspACI CCGC 2 cut(s) 176, 345
BspANI GGCC 3 cut(s) 1028, 1119, 1157
BspCNI CTCAG 3 cut(s) 785, 874, 1002
BspDI ATCGAT 1 cut(s) 426
BspLI GGNNCC 2 cut(s) 942, 965
BspTI CTTAAG 1 cut(s) 580
BspTNI GGTCTC 2 cut(s) 619, 980
BsrFI RCCGGY 1 cut(s) 104
BsrGI TGTACA 1 cut(s) 85
BsrI ACTGG 3 cut(s) 753, 908, 1120
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 3 cut(s) 165, 700, 1029
BssMI GATC 1 cut(s) 153
BssNAI GTATAC 1 cut(s) 555
BssT1I CCWWGG 2 cut(s) 165, 1029
Bst1107I GTATAC 1 cut(s) 555
Bst2UI CCWGG 2 cut(s) 961, 1275
Bst4CI ACNGT 4 cut(s) 90, 287, 562, 1057
Bst6I CTCTTC 2 cut(s) 279, 324
BstAFI CTTAAG 1 cut(s) 580
BstAUI TGTACA 1 cut(s) 85
BstBAI YACGTR 1 cut(s) 473
BstC8I GCNNGC 1 cut(s) 347
BstDEI CTNAG 5 cut(s) 628, 722, 772, 861, 1010
BstF5I GGATG 4 cut(s) 106, 457, 590, 835
BstH2I RGCGCY 1 cut(s) 184
BstHHI GCGC 1 cut(s) 183
BstKTI GATC 1 cut(s) 156
BstMAI GTCTC 5 cut(s) 276, 619, 747, 775, 980
BstMBI GATC 1 cut(s) 153
BstMWI GCNNNNNNNGC 1 cut(s) 857
BstNI CCWGG 2 cut(s) 961, 1275
BstNSI RCATGY 1 cut(s) 326
BstSCI CCNGG 2 cut(s) 959, 1273
BstSFI CTRYAG 1 cut(s) 558
BstV1I GCAGC 3 cut(s) 58, 788, 1106
BstZ17I GTATAC 1 cut(s) 555
Bsu15I ATCGAT 1 cut(s) 426
BsuRI GGCC 3 cut(s) 1028, 1119, 1157
BsuTUI ATCGAT 1 cut(s) 426
BtsCI GGATG 4 cut(s) 106, 457, 590, 835
BtsI GCAGTG 1 cut(s) 592
BtsIMutI CAGTG 2 cut(s) 592, 667
Cac8I GCNNGC 1 cut(s) 347
CfoI GCGC 1 cut(s) 183
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 2 cut(s) 1026, 1289
ClaI ATCGAT 1 cut(s) 426
CsiI ACCWGGT 1 cut(s) 1273
Csp6I GTAC 4 cut(s) 86, 474, 784, 920
CspCI CAANNNNNGTGG 2 cut(s) 259, 294
CviAII CATG 4 cut(s) 323, 350, 676, 1121
CviQI GTAC 4 cut(s) 86, 474, 784, 920
DdeI CTNAG 5 cut(s) 628, 722, 772, 861, 1010
DpnI GATC 1 cut(s) 155
DpnII GATC 1 cut(s) 153
EaeI YGGCCR 2 cut(s) 1117, 1155
Eam1104I CTCTTC 2 cut(s) 279, 324
EarI CTCTTC 2 cut(s) 279, 324
Eco130I CCWWGG 2 cut(s) 165, 1029
Eco31I GGTCTC 2 cut(s) 619, 980
Eco32I GATATC 1 cut(s) 405
Eco47I GGWCC 1 cut(s) 1289
Eco88I CYCGRG 1 cut(s) 477
EcoRII CCWGG 2 cut(s) 959, 1273
EcoRV GATATC 1 cut(s) 405
EcoT14I CCWWGG 2 cut(s) 165, 1029
EcoT22I ATGCAT 1 cut(s) 316
ErhI CCWWGG 2 cut(s) 165, 1029
Esp3I CGTCTC 1 cut(s) 276
FaeI CATG 4 cut(s) 326, 353, 679, 1124
FaqI GGGAC 1 cut(s) 97
FatI CATG 4 cut(s) 322, 349, 675, 1120
FauI CCCGC 2 cut(s) 169, 338
FauNDI CATATG 1 cut(s) 134
FblI GTMKAC 2 cut(s) 554, 1104
Fnu4HI GCNGC 3 cut(s) 72, 777, 1095
FokI GGATG 4 cut(s) 113, 464, 577, 842
Fsp4HI GCNGC 3 cut(s) 72, 777, 1095
FspBI CTAG 3 cut(s) 75, 852, 1226
GlaI GCGC 1 cut(s) 182
GluI GCNGC 3 cut(s) 72, 777, 1095
HaeII RGCGCY 1 cut(s) 184
HaeIII GGCC 3 cut(s) 1028, 1119, 1157
HapII CCGG 1 cut(s) 105
HhaI GCGC 1 cut(s) 183
Hin1II CATG 4 cut(s) 326, 353, 679, 1124
Hin6I GCGC 1 cut(s) 181
HinP1I GCGC 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 653
HinfI GANTC 6 cut(s) 194, 257, 412, 707, 1106, 1295
HpaII CCGG 1 cut(s) 105
HphI GGTGA 5 cut(s) 299, 595, 644, 661, 716
Hpy166II GTNNAC 4 cut(s) 555, 603, 1105, 1271
Hpy188I TCNGA 6 cut(s) 203, 262, 421, 872, 1011, 1195
Hpy188III TCNNGA 2 cut(s) 711, 1292
Hpy8I GTNNAC 4 cut(s) 555, 603, 1105, 1271
HpyAV CCTTC 2 cut(s) 5, 1200
HpyCH4III ACNGT 4 cut(s) 90, 287, 562, 1057
HpyCH4IV ACGT 2 cut(s) 472, 1005
HpyCH4V TGCA 9 cut(s) 56, 138, 314, 322, 590, 623, 675, 728, 925
HpyF10VI GCNNNNNNNGC 1 cut(s) 857
HpyF3I CTNAG 5 cut(s) 628, 722, 772, 861, 1010
HpySE526I ACGT 2 cut(s) 472, 1005
Hsp92II CATG 4 cut(s) 326, 353, 679, 1124
HspAI GCGC 1 cut(s) 181
Kzo9I GATC 1 cut(s) 153
LmnI GCTCC 4 cut(s) 262, 1129, 1148, 1217
Lsp1109I GCAGC 3 cut(s) 58, 788, 1106
LweI GCATC 7 cut(s) 65, 91, 280, 301, 323, 599, 1186
MabI ACCWGGT 1 cut(s) 1273
MaeI CTAG 3 cut(s) 75, 852, 1226
MaeII ACGT 2 cut(s) 472, 1005
MaeIII GTNAC 4 cut(s) 562, 704, 816, 1111
MalI GATC 1 cut(s) 155
MboI GATC 1 cut(s) 153
MboII GAAGA 5 cut(s) 266, 341, 659, 896, 1031
MfeI CAATTG 2 cut(s) 729, 1098
MlsI TGGCCA 1 cut(s) 1119
MluCI AATT 7 cut(s) 139, 538, 690, 729, 1098, 1229, 1263
MluNI TGGCCA 1 cut(s) 1119
MlyI GAGTC 3 cut(s) 266, 701, 1100
MmeI TCCRAC 3 cut(s) 240, 446, 918
Mox20I TGGCCA 1 cut(s) 1119
Mph1103I ATGCAT 1 cut(s) 316
MroXI GAANNNNTTC 1 cut(s) 656
MscI TGGCCA 1 cut(s) 1119
MseI TTAA 3 cut(s) 581, 896, 1182
MslI CAYNNNNRTG 1 cut(s) 647
Msp20I TGGCCA 1 cut(s) 1119
MspA1I CMGCKG 1 cut(s) 1067
MspCI CTTAAG 1 cut(s) 580
MspI CCGG 1 cut(s) 105
MspR9I CCNGG 2 cut(s) 961, 1275
MunI CAATTG 2 cut(s) 729, 1098
Mva1269I GAATGC 1 cut(s) 623
MvaI CCWGG 2 cut(s) 961, 1275
MwoI GCNNNNNNNGC 1 cut(s) 857
NdeI CATATG 1 cut(s) 134
NdeII GATC 1 cut(s) 153
NlaIII CATG 4 cut(s) 326, 353, 679, 1124
NlaIV GGNNCC 2 cut(s) 942, 965
NmuCI GTSAC 3 cut(s) 562, 704, 1111
NsiI ATGCAT 1 cut(s) 316
NspI RCATGY 1 cut(s) 326
OliI CACNNNNGTG 1 cut(s) 647
PaeR7I CTCGAG 1 cut(s) 477
PcsI WCGNNNNNNNCGW 1 cut(s) 1011
PctI GAATGC 1 cut(s) 623
PdmI GAANNNNTTC 1 cut(s) 656
PfeI GAWTC 3 cut(s) 194, 412, 1295
PkrI GCNGC 3 cut(s) 73, 778, 1096
PleI GAGTC 3 cut(s) 265, 701, 1100
PpsI GAGTC 3 cut(s) 265, 701, 1100
Ppu21I YACGTR 1 cut(s) 473
PshBI ATTAAT 1 cut(s) 1182
Psp1406I AACGTT 1 cut(s) 1005
Psp6I CCWGG 2 cut(s) 959, 1273
PspGI CCWGG 2 cut(s) 959, 1273
PspN4I GGNNCC 2 cut(s) 942, 965
PspPI GGNCC 2 cut(s) 1026, 1289
PspXI VCTCGAGB 1 cut(s) 477
PvuII CAGCTG 1 cut(s) 1067
RsaI GTAC 4 cut(s) 87, 475, 785, 921
RsaNI GTAC 4 cut(s) 86, 474, 784, 920
RseI CAYNNNNRTG 1 cut(s) 647
SaqAI TTAA 3 cut(s) 581, 896, 1182
SatI GCNGC 3 cut(s) 72, 777, 1095
Sau3AI GATC 1 cut(s) 153
Sau96I GGNCC 2 cut(s) 1026, 1289
SchI GAGTC 3 cut(s) 266, 701, 1100
ScrFI CCNGG 2 cut(s) 961, 1275
SexAI ACCWGGT 1 cut(s) 1273
SfaNI GCATC 7 cut(s) 65, 91, 280, 301, 323, 599, 1186
SfcI CTRYAG 1 cut(s) 558
Sfr274I CTCGAG 1 cut(s) 477
SinI GGWCC 1 cut(s) 1289
SlaI CTCGAG 1 cut(s) 477
SmiMI CAYNNNNRTG 1 cut(s) 647
SmlI CTYRAG 2 cut(s) 477, 580
SmoI CTYRAG 2 cut(s) 477, 580
Sse9I AATT 7 cut(s) 139, 538, 690, 729, 1098, 1229, 1263
SsiI CCGC 2 cut(s) 176, 345
SspI AATATT 2 cut(s) 900, 1186
SspMI CTAG 3 cut(s) 75, 852, 1226
StyD4I CCNGG 2 cut(s) 959, 1273
StyI CCWWGG 2 cut(s) 165, 1029
TaaI ACNGT 4 cut(s) 90, 287, 562, 1057
TaiI ACGT 2 cut(s) 475, 1008
TaqI TCGA 3 cut(s) 426, 478, 1211
TaqII GACCGA 1 cut(s) 962
TasI AATT 7 cut(s) 139, 538, 690, 729, 1098, 1229, 1263
TatI WGTACW 3 cut(s) 85, 783, 919
TfiI GAWTC 3 cut(s) 194, 412, 1295
Tru1I TTAA 3 cut(s) 581, 896, 1182
Tru9I TTAA 3 cut(s) 581, 896, 1182
TscAI CASTG 2 cut(s) 592, 667
TseFI GTSAC 3 cut(s) 562, 704, 1111
TseI GCWGC 3 cut(s) 71, 776, 1094
Tsp45I GTSAC 3 cut(s) 562, 704, 1111
TspDTI ATGAA 6 cut(s) 17, 132, 444, 468, 1272, 1299
TspGWI ACGGA 3 cut(s) 228, 1077, 1099
TspRI CASTG 2 cut(s) 592, 667
Vha464I CTTAAG 1 cut(s) 580
VpaK11BI GGWCC 1 cut(s) 1289
VspI ATTAAT 1 cut(s) 1182
XceI RCATGY 1 cut(s) 326
XhoI CTCGAG 1 cut(s) 477
XmiI GTMKAC 2 cut(s) 554, 1104
XmnI GAANNNNTTC 1 cut(s) 656
XspI CTAG 3 cut(s) 75, 852, 1226
Zsp2I ATGCAT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.