Rh6CG283500

Thiamine-repressible mitochondrial transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0005884
Physical Location & Seq
Reverse (-)
46124107 .. 46128131
4025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG283500.1

Sequence Viewer

Length: 999 bp
ATGAAAAGTGAAGGTTTGAAGTGGGTTTTAGGTTTGATATACATATTTGCTGTTGCATCTATTTGGATAGCTGCTAGTTTTGTCGTACAGTCGGTTGTGGATGCCGGTGTGTCCCCTTTTCTTATTACCTACATATGCAATTCATTGTTTGTGATCTACATTCCCTTGGTTGAAGCCGGGCGCTATTTGGAGGATTCGTTCGGAGGGTTTTGGTTTTGGAAAAGTAAGGAGAGTAGTCCTTTGCGAGAGTTGGTGGAGTCGGAGCAATCCACTCTTCTTGGAGACGGTGATGCTGTTGCGAAAACTGATGATGCTTCTGTGCATGTAGAAGAGGGAGAAATTGGCGGGCATGGAAAAGTTATTGATGGAGAAGTGAAAGTTGTGGCGTATGAGCCTATTAGGATATCAGCGAATCTGTCTGAAATCGATGAAGTTGCTGATAAGCAAGTGGATGAAAAAGGGCGTTGGACACGTACTCGAGTGGCAAAAGTTAGCCTATTGATTTCCCCATTTTGGTTTTTCGCACAGCTCACTTTCAATTTGTCATTGAAGTATACTACAGTCACATCAAATACCATCTTAAGCAGTGCATCCAGCCTGTTCACCTTTTTGGTCTCTCTTGCATTCTTAGGTGAGAACTTCACTTTGGTGAAGCTTTTCAGTGTTCTTCTTTGCATGGGAGGAACGATAATTGTCAGCCTCGGTGACTCACGAACTACTCTAAGTGCAATTGCTTCAAACCCTCTACTGGGAGACATACTTGCTCTTGTCTCAGCAGCTTTGTATTCTGTGTATATTACCCTCATTCGCAAGAAGTTACCTGATGAGGATGATGAGAAAAGTGGTCGTGCTAGTATGGCTCAGTTTCTCGGATTTTTAGGGCTTTCTAACCTTTTAATATTTCTTCCAGTTGCCCTTGTACTGCATTTCTCAAAGTTGGAACCCTTTTATATGCTGACCTGGAAGCAGCTTGGTCTCATTGTTGGTAAAGGTTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

332

Amino Acids

36.34

Weight (kDa)

5.14

Isoelectric Point (pI)

30.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 147 - 271 2.9e-07 Purine nucleobase transmembrane transport
EamA PF00892 164 - 233 3.1e-09 EamA-like transporter family
TPT PF03151 169 - 310 1.7e-06 Triose-phosphate Transporter family
SLC35F PF06027 183 - 267 6.6e-09 Solute carrier family 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 554
AciI CCGC 1 cut(s) 345
AfaI GTAC 3 cut(s) 87, 475, 921
AfiI CCNNNNNNNGG 3 cut(s) 513, 748, 749
AflII CTTAAG 1 cut(s) 580
AflIII ACRYGT 1 cut(s) 470
AgsI TTSAA 5 cut(s) 19, 173, 538, 550, 738
AjnI CCWGG 1 cut(s) 959
AjuI GAANNNNNNNTTGG 2 cut(s) 629, 661
AleI CACNNNNGTG 1 cut(s) 647
AloI GAACNNNNNNTCC 2 cut(s) 182, 214
AluBI AGCT 5 cut(s) 71, 529, 655, 779, 970
AluI AGCT 5 cut(s) 71, 529, 655, 779, 970
Alw26I GTCTC 5 cut(s) 276, 619, 747, 775, 980
Ama87I CYCGRG 1 cut(s) 477
ApeKI GCWGC 3 cut(s) 71, 776, 967
Asp700I GAANNNNTTC 1 cut(s) 656
AspLEI GCGC 1 cut(s) 183
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 5 cut(s) 299, 595, 644, 661, 716
AvaI CYCGRG 1 cut(s) 477
BbvI GCAGC 3 cut(s) 58, 788, 979
BccI CCATC 2 cut(s) 359, 584
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BciT130I CCWGG 1 cut(s) 961
BcnI CCSGG 1 cut(s) 178
BcoDI GTCTC 5 cut(s) 276, 619, 747, 775, 980
BfaI CTAG 2 cut(s) 75, 852
BfmI CTRYAG 1 cut(s) 558
BfoI RGCGCY 1 cut(s) 184
BfrI CTTAAG 1 cut(s) 580
BisI GCNGC 3 cut(s) 72, 777, 968
BlsI GCNGC 3 cut(s) 73, 778, 969
Bme1390I CCNGG 2 cut(s) 178, 961
BmeT110I CYCGRG 1 cut(s) 477
BmiI GGNNCC 1 cut(s) 942
BmrFI CCNGG 2 cut(s) 178, 961
BmrI ACTGGG 1 cut(s) 758
BmsI GCATC 5 cut(s) 65, 91, 280, 301, 599
BmuI ACTGGG 1 cut(s) 758
BpuMI CCSGG 1 cut(s) 178
Bsa29I ATCGAT 1 cut(s) 426
BsaAI YACGTR 1 cut(s) 473
BsaI GGTCTC 2 cut(s) 619, 980
BsaJI CCNNGG 2 cut(s) 165, 700
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 3 cut(s) 513, 748, 749
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 753, 908
BseBI CCWGG 1 cut(s) 961
BseCI ATCGAT 1 cut(s) 426
BseDI CCNNGG 2 cut(s) 165, 700
BseGI GGATG 4 cut(s) 106, 457, 590, 835
BseLI CCNNNNNNNGG 3 cut(s) 513, 748, 749
BseMII CTCAG 2 cut(s) 786, 875
BseNI ACTGG 2 cut(s) 753, 908
BseXI GCAGC 3 cut(s) 58, 788, 979
BshVI ATCGAT 1 cut(s) 426
BsiHKCI CYCGRG 1 cut(s) 477
BsiSI CCGG 2 cut(s) 105, 177
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 3 cut(s) 513, 748, 749
BsmAI GTCTC 5 cut(s) 276, 619, 747, 775, 980
BsmBI CGTCTC 1 cut(s) 276
BsmFI GGGAC 1 cut(s) 97
BsmI GAATGC 1 cut(s) 623
Bso31I GGTCTC 2 cut(s) 619, 980
BsoBI CYCGRG 1 cut(s) 477
Bsp143I GATC 1 cut(s) 153
BspACI CCGC 1 cut(s) 345
BspCNI CTCAG 2 cut(s) 785, 874
BspDI ATCGAT 1 cut(s) 426
BspLI GGNNCC 1 cut(s) 942
BspTI CTTAAG 1 cut(s) 580
BspTNI GGTCTC 2 cut(s) 619, 980
BsrFI RCCGGY 1 cut(s) 104
BsrI ACTGG 2 cut(s) 753, 908
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 2 cut(s) 165, 700
BssMI GATC 1 cut(s) 153
BssNAI GTATAC 1 cut(s) 555
BssT1I CCWWGG 1 cut(s) 165
Bst1107I GTATAC 1 cut(s) 555
Bst2UI CCWGG 1 cut(s) 961
Bst4CI ACNGT 3 cut(s) 90, 287, 562
Bst6I CTCTTC 2 cut(s) 279, 324
BstAFI CTTAAG 1 cut(s) 580
BstBAI YACGTR 1 cut(s) 473
BstC8I GCNNGC 1 cut(s) 347
BstDEI CTNAG 4 cut(s) 628, 722, 772, 861
BstF5I GGATG 4 cut(s) 106, 457, 590, 835
BstH2I RGCGCY 1 cut(s) 184
BstHHI GCGC 1 cut(s) 183
BstKTI GATC 1 cut(s) 156
BstMAI GTCTC 5 cut(s) 276, 619, 747, 775, 980
BstMBI GATC 1 cut(s) 153
BstMWI GCNNNNNNNGC 1 cut(s) 857
BstNI CCWGG 1 cut(s) 961
BstNSI RCATGY 1 cut(s) 326
BstSCI CCNGG 2 cut(s) 176, 959
BstSFI CTRYAG 1 cut(s) 558
BstV1I GCAGC 3 cut(s) 58, 788, 979
BstZ17I GTATAC 1 cut(s) 555
Bsu15I ATCGAT 1 cut(s) 426
BsuTUI ATCGAT 1 cut(s) 426
BtsCI GGATG 4 cut(s) 106, 457, 590, 835
BtsI GCAGTG 1 cut(s) 592
BtsIMutI CAGTG 2 cut(s) 592, 667
Cac8I GCNNGC 1 cut(s) 347
CfoI GCGC 1 cut(s) 183
Cfr10I RCCGGY 1 cut(s) 104
ClaI ATCGAT 1 cut(s) 426
Csp6I GTAC 3 cut(s) 86, 474, 920
CspCI CAANNNNNGTGG 2 cut(s) 259, 294
CviAII CATG 3 cut(s) 323, 350, 676
CviQI GTAC 3 cut(s) 86, 474, 920
DdeI CTNAG 4 cut(s) 628, 722, 772, 861
DpnI GATC 1 cut(s) 155
DpnII GATC 1 cut(s) 153
Eam1104I CTCTTC 2 cut(s) 279, 324
EarI CTCTTC 2 cut(s) 279, 324
Eco130I CCWWGG 1 cut(s) 165
Eco31I GGTCTC 2 cut(s) 619, 980
Eco32I GATATC 1 cut(s) 405
Eco88I CYCGRG 1 cut(s) 477
EcoRII CCWGG 1 cut(s) 959
EcoRV GATATC 1 cut(s) 405
EcoT14I CCWWGG 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 165
Esp3I CGTCTC 1 cut(s) 276
FaeI CATG 3 cut(s) 326, 353, 679
FaqI GGGAC 1 cut(s) 97
FatI CATG 3 cut(s) 322, 349, 675
FauI CCCGC 1 cut(s) 338
FauNDI CATATG 1 cut(s) 134
FblI GTMKAC 1 cut(s) 554
Fnu4HI GCNGC 3 cut(s) 72, 777, 968
FokI GGATG 4 cut(s) 113, 464, 577, 842
Fsp4HI GCNGC 3 cut(s) 72, 777, 968
FspBI CTAG 2 cut(s) 75, 852
GlaI GCGC 1 cut(s) 182
GluI GCNGC 3 cut(s) 72, 777, 968
HaeII RGCGCY 1 cut(s) 184
HapII CCGG 2 cut(s) 105, 177
HhaI GCGC 1 cut(s) 183
Hin1II CATG 3 cut(s) 326, 353, 679
Hin6I GCGC 1 cut(s) 181
HinP1I GCGC 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 653
HinfI GANTC 4 cut(s) 194, 257, 412, 707
HpaII CCGG 2 cut(s) 105, 177
HphI GGTGA 5 cut(s) 299, 595, 644, 661, 716
Hpy166II GTNNAC 2 cut(s) 555, 603
Hpy188I TCNGA 4 cut(s) 203, 262, 421, 872
Hpy188III TCNNGA 1 cut(s) 711
Hpy8I GTNNAC 2 cut(s) 555, 603
HpyAV CCTTC 1 cut(s) 5
HpyCH4III ACNGT 3 cut(s) 90, 287, 562
HpyCH4IV ACGT 1 cut(s) 472
HpyCH4V TGCA 8 cut(s) 56, 138, 322, 590, 623, 675, 728, 925
HpyF10VI GCNNNNNNNGC 1 cut(s) 857
HpyF3I CTNAG 4 cut(s) 628, 722, 772, 861
HpySE526I ACGT 1 cut(s) 472
Hsp92II CATG 3 cut(s) 326, 353, 679
HspAI GCGC 1 cut(s) 181
Kzo9I GATC 1 cut(s) 153
LmnI GCTCC 1 cut(s) 262
LpnPI CCDG 9 cut(s) 118, 190, 607, 611, 734, 834, 921, 946, 973
Lsp1109I GCAGC 3 cut(s) 58, 788, 979
LweI GCATC 5 cut(s) 65, 91, 280, 301, 599
MaeI CTAG 2 cut(s) 75, 852
MaeII ACGT 1 cut(s) 472
MaeIII GTNAC 3 cut(s) 562, 704, 816
MalI GATC 1 cut(s) 155
MboI GATC 1 cut(s) 153
MboII GAAGA 4 cut(s) 266, 341, 659, 896
MfeI CAATTG 1 cut(s) 729
MluCI AATT 5 cut(s) 139, 339, 538, 690, 729
MlyI GAGTC 2 cut(s) 266, 701
MmeI TCCRAC 3 cut(s) 240, 446, 918
MnlI CCTC 8 cut(s) 184, 197, 325, 674, 710, 753, 812, 820
MroXI GAANNNNTTC 1 cut(s) 656
MseI TTAA 2 cut(s) 581, 896
MslI CAYNNNNRTG 1 cut(s) 647
MspCI CTTAAG 1 cut(s) 580
MspI CCGG 2 cut(s) 105, 177
MspR9I CCNGG 2 cut(s) 178, 961
MunI CAATTG 1 cut(s) 729
Mva1269I GAATGC 1 cut(s) 623
MvaI CCWGG 1 cut(s) 961
MwoI GCNNNNNNNGC 1 cut(s) 857
NciI CCSGG 1 cut(s) 178
NdeI CATATG 1 cut(s) 134
NdeII GATC 1 cut(s) 153
NlaIII CATG 3 cut(s) 326, 353, 679
NlaIV GGNNCC 1 cut(s) 942
NmuCI GTSAC 2 cut(s) 562, 704
NspI RCATGY 1 cut(s) 326
OliI CACNNNNGTG 1 cut(s) 647
PaeR7I CTCGAG 1 cut(s) 477
PctI GAATGC 1 cut(s) 623
PdmI GAANNNNTTC 1 cut(s) 656
PfeI GAWTC 2 cut(s) 194, 412
PkrI GCNGC 3 cut(s) 73, 778, 969
PleI GAGTC 2 cut(s) 265, 701
PpsI GAGTC 2 cut(s) 265, 701
Ppu21I YACGTR 1 cut(s) 473
Psp6I CCWGG 1 cut(s) 959
PspGI CCWGG 1 cut(s) 959
PspN4I GGNNCC 1 cut(s) 942
PspXI VCTCGAGB 1 cut(s) 477
RsaI GTAC 3 cut(s) 87, 475, 921
RsaNI GTAC 3 cut(s) 86, 474, 920
RseI CAYNNNNRTG 1 cut(s) 647
SaqAI TTAA 2 cut(s) 581, 896
SatI GCNGC 3 cut(s) 72, 777, 968
Sau3AI GATC 1 cut(s) 153
SchI GAGTC 2 cut(s) 266, 701
ScrFI CCNGG 2 cut(s) 178, 961
SfaNI GCATC 5 cut(s) 65, 91, 280, 301, 599
SfcI CTRYAG 1 cut(s) 558
Sfr274I CTCGAG 1 cut(s) 477
SlaI CTCGAG 1 cut(s) 477
SmiMI CAYNNNNRTG 1 cut(s) 647
SmlI CTYRAG 2 cut(s) 477, 580
SmoI CTYRAG 2 cut(s) 477, 580
Sse9I AATT 5 cut(s) 139, 339, 538, 690, 729
SsiI CCGC 1 cut(s) 345
SspI AATATT 1 cut(s) 900
SspMI CTAG 2 cut(s) 75, 852
StyD4I CCNGG 2 cut(s) 176, 959
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 3 cut(s) 90, 287, 562
TaiI ACGT 1 cut(s) 475
TaqI TCGA 2 cut(s) 426, 478
TasI AATT 5 cut(s) 139, 339, 538, 690, 729
TatI WGTACW 1 cut(s) 919
TfiI GAWTC 2 cut(s) 194, 412
Tru1I TTAA 2 cut(s) 581, 896
Tru9I TTAA 2 cut(s) 581, 896
TscAI CASTG 2 cut(s) 592, 667
TseFI GTSAC 2 cut(s) 562, 704
TseI GCWGC 3 cut(s) 71, 776, 967
Tsp45I GTSAC 2 cut(s) 562, 704
TspDTI ATGAA 4 cut(s) 17, 132, 444, 468
TspRI CASTG 2 cut(s) 592, 667
Vha464I CTTAAG 1 cut(s) 580
XceI RCATGY 1 cut(s) 326
XhoI CTCGAG 1 cut(s) 477
XmiI GTMKAC 1 cut(s) 554
XmnI GAANNNNTTC 1 cut(s) 656
XspI CTAG 2 cut(s) 75, 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.