MD10G1165300.v1.1

Thiamine-repressible mitochondrial transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005884
Physical Location & Seq
Reverse (-)
25600548 .. 25602813
2266 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1165300.v1.1.491

Sequence Viewer

Length: 1278 bp
ATGAAAAGTGAAGTTGGGAAGTGGGTATTAGGTTTGGTATACATATTTGCTGTTGCATCTATCTGGATAGCTGCCAGTTTCGTAGTACAGTCGGTTGTAGATGCCGGTGTGTCCCCGTTCCTCATCACATACATATGCAATTCTCTGTTTGTGATCTACATTCCCTTGGTTGAAATCGGGCGGTATTTGGAGGATAATTTTGAGGGGTTTTGGATTTGGAGAAGTAGGAAGAGTAGCCCTTTGCAAAGATTAGGAGATTCGGAGCAAATCACTCTCCTTGAGGAGACTGACGGAGTTGTGAAAGCAGATGCGGAAGAGGGGGAAGTGAATCTTGGTGCAGAACCGAAAGTTGTATTGTACGAGCTTGTTGGAACTTCAGCAACTCAAGACAATGGCACTGAAACGGTAGATAAGCAAGTGGATGAAAAAGGGCGTTGGACACGAACTCGAGTGGCTAAAGTTAGCCTGCTGATTTCCCCGTTTTGGTTTTTCGCACAGCTGACTTTCAATTTGTCATTGAAGTATACTACGGTTACGTCGAATACAATCTTAAGCAGTTCATCCAGCCTTTTCACCTTTTTGGTCGCCCTAGTATTCTTAGGGGAGAAGTTTACTTTGGTTAAGCTTATTAGCGTTCTTCTTTGCATGGGAGGAACGATAATTGTCAGCCTTGGTGACTCACGAACTGCTCTAAGTGCAATTGCTTCAAACCCTCTCCTCGGCGACATACTTGCTCTTGTCTCGGCGGCCTTCTATTCTGTGTATATTACCCTCATTCGCAAGAAATTACCTGATGAGGATGATGAAAAAAGCGGCCGTGCCAGTATGGCTCAGTTTCTAGGATTTCTAGGGCTTTCCAACCTTCTTATATTTCTTCCGGTTGCCCTTATACTTCATTTCTCCAAGCTGGAGCCGTTTTACATGCTTACCTGGGAGCAGGTCGGTCTCATCGTTGGTAAAGGTTTGTTGGATAATGTTCTGAGCGACTACTTATGGGCCAAGGCCGTTCTTCTAACCACCACGACGGTAGCAACGGCCGGTCTGACCATCCAGGTTCCGCTGGCGGCAATTGTTGACTCAATGACTGGCCATGCTCCTCATTTTGCCGATTACCTTGGAGCCGTGGCGGTCATGATCGGATTCGCAGGCATAAACATTCCCGCTGATGCTTTTAACAGACCAAAAGAAGCTATACTTGCGAGAGAGAGTGAAAATATCAGTGCAACCGGTGAAGCTCGCAATTCATCAGGCAGCCCGATTTCAGCTGCCCCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

426

Amino Acids

46.03

Weight (kDa)

4.97

Isoelectric Point (pI)

32.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 140 - 261 9.4e-08 Purine nucleobase transmembrane transport
EamA PF00892 154 - 223 1e-09 EamA-like transporter family
TPT PF03151 159 - 305 5.7e-06 Triose-phosphate Transporter family
SLC35F PF06027 173 - 290 7.8e-09 Solute carrier family 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 928
AccI GTMKAC 2 cut(s) 39, 524
AciI CCGC 8 cut(s) 181, 311, 746, 813, 1058, 1064, 1127, 1161
AcoI YGGCCR 3 cut(s) 814, 1035, 1087
AcuI CTGAAG 1 cut(s) 360
AfaI GTAC 2 cut(s) 87, 359
AfiI CCNNNNNNNGG 2 cut(s) 483, 719
AflII CTTAAG 1 cut(s) 550
AgeI ACCGGT 1 cut(s) 1226
AgsI TTSAA 4 cut(s) 173, 508, 520, 708
AjnI CCWGG 2 cut(s) 929, 1050
AjuI GAANNNNNNNTTGG 4 cut(s) 315, 347, 599, 631
AluBI AGCT 8 cut(s) 71, 364, 499, 625, 907, 1190, 1235, 1265
AluI AGCT 8 cut(s) 71, 364, 499, 625, 907, 1190, 1235, 1265
Alw26I GTCTC 3 cut(s) 278, 745, 950
Ama87I CYCGRG 1 cut(s) 447
AoxI GGCC 6 cut(s) 747, 814, 996, 1002, 1035, 1087
ApeKI GCWGC 3 cut(s) 71, 1251, 1265
ArsI GACNNNNNNTTYG 2 cut(s) 493, 525
AsiGI ACCGGT 1 cut(s) 1226
AspS9I GGNCC 1 cut(s) 996
AsuHPI GGTGA 3 cut(s) 565, 686, 1241
AvaI CYCGRG 1 cut(s) 447
BalI TGGCCA 1 cut(s) 1089
BbvI GCAGC 3 cut(s) 58, 1252, 1263
BccI CCATC 1 cut(s) 1055
BceAI ACGGC 5 cut(s) 801, 898, 989, 1050, 1106
BcgI CGANNNNNNTGC 2 cut(s) 713, 747
BciT130I CCWGG 2 cut(s) 931, 1052
BcoDI GTCTC 3 cut(s) 278, 745, 950
BfaI CTAG 3 cut(s) 590, 839, 848
BfrI CTTAAG 1 cut(s) 550
BfuAI ACCTGC 1 cut(s) 928
BglI GCCNNNNNGGC 1 cut(s) 827
BisI GCNGC 6 cut(s) 72, 747, 814, 1065, 1252, 1266
BlsI GCNGC 6 cut(s) 73, 748, 815, 1066, 1253, 1267
Bme1390I CCNGG 2 cut(s) 931, 1052
BmeT110I CYCGRG 1 cut(s) 447
BmgT120I GGNCC 1 cut(s) 996
BmiI GGNNCC 3 cut(s) 912, 1056, 1120
BmrFI CCNGG 2 cut(s) 931, 1052
BmsI GCATC 4 cut(s) 65, 91, 298, 1156
BpmI CTGGAG 1 cut(s) 929
BpuEI CTTGAG 2 cut(s) 299, 369
BsaI GGTCTC 1 cut(s) 950
BsaJI CCNNGG 7 cut(s) 165, 670, 718, 930, 999, 1114, 1122
BsaWI WCCGGW 2 cut(s) 877, 1226
Bsc4I CCNNNNNNNGG 2 cut(s) 483, 719
Bse118I RCCGGY 3 cut(s) 104, 1037, 1226
Bse1I ACTGG 3 cut(s) 75, 822, 1090
BseBI CCWGG 2 cut(s) 931, 1052
BseDI CCNNGG 7 cut(s) 165, 670, 718, 930, 999, 1114, 1122
BseGI GGATG 4 cut(s) 427, 560, 805, 1047
BseLI CCNNNNNNNGG 2 cut(s) 483, 719
BseMII CTCAG 2 cut(s) 845, 971
BseNI ACTGG 3 cut(s) 75, 822, 1090
BseRI GAGGAG 3 cut(s) 296, 707, 1086
BseX3I CGGCCG 2 cut(s) 814, 1035
BseXI GCAGC 3 cut(s) 58, 1252, 1263
BsgI GTGCAG 1 cut(s) 357
Bsh1285I CGRYCG 2 cut(s) 817, 1038
BshFI GGCC 6 cut(s) 749, 816, 998, 1004, 1037, 1089
BshTI ACCGGT 1 cut(s) 1226
BsiEI CGRYCG 2 cut(s) 817, 1038
BsiHKCI CYCGRG 1 cut(s) 447
BsiSI CCGG 4 cut(s) 105, 878, 1038, 1227
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 2 cut(s) 483, 719
BsmAI GTCTC 3 cut(s) 278, 745, 950
BsmFI GGGAC 1 cut(s) 97
BsnI GGCC 6 cut(s) 749, 816, 998, 1004, 1037, 1089
Bso31I GGTCTC 1 cut(s) 950
BsoBI CYCGRG 1 cut(s) 447
Bsp143I GATC 2 cut(s) 153, 1134
BspACI CCGC 8 cut(s) 181, 311, 746, 813, 1058, 1064, 1127, 1161
BspANI GGCC 6 cut(s) 749, 816, 998, 1004, 1037, 1089
BspCNI CTCAG 2 cut(s) 844, 972
BspHI TCATGA 1 cut(s) 1131
BspLI GGNNCC 3 cut(s) 912, 1056, 1120
BspMI ACCTGC 1 cut(s) 928
BspTI CTTAAG 1 cut(s) 550
BspTNI GGTCTC 1 cut(s) 950
BsrFI RCCGGY 3 cut(s) 104, 1037, 1226
BsrI ACTGG 3 cut(s) 75, 822, 1090
BssAI RCCGGY 3 cut(s) 104, 1037, 1226
BssECI CCNNGG 7 cut(s) 165, 670, 718, 930, 999, 1114, 1122
BssMI GATC 2 cut(s) 153, 1134
BssNAI GTATAC 2 cut(s) 40, 525
BssT1I CCWWGG 4 cut(s) 165, 670, 999, 1114
Bst1107I GTATAC 2 cut(s) 40, 525
Bst2UI CCWGG 2 cut(s) 931, 1052
Bst4CI ACNGT 4 cut(s) 90, 406, 532, 1027
Bst6I CTCTTC 2 cut(s) 224, 309
BstAFI CTTAAG 1 cut(s) 550
BstC8I GCNNGC 4 cut(s) 467, 1062, 1147, 1237
BstDEI CTNAG 4 cut(s) 598, 692, 831, 980
BstDSI CCRYGG 1 cut(s) 1122
BstF5I GGATG 4 cut(s) 427, 560, 805, 1047
BstKTI GATC 2 cut(s) 156, 1137
BstMAI GTCTC 3 cut(s) 278, 745, 950
BstMBI GATC 2 cut(s) 153, 1134
BstMCI CGRYCG 2 cut(s) 817, 1038
BstMWI GCNNNNNNNGC 3 cut(s) 695, 827, 1196
BstNI CCWGG 2 cut(s) 931, 1052
BstNSI RCATGY 1 cut(s) 925
BstSCI CCNGG 2 cut(s) 929, 1050
BstV1I GCAGC 3 cut(s) 58, 1252, 1263
BstZ17I GTATAC 2 cut(s) 40, 525
BstZI CGGCCG 2 cut(s) 814, 1035
BsuRI GGCC 6 cut(s) 749, 816, 998, 1004, 1037, 1089
BtgI CCRYGG 1 cut(s) 1122
BtsCI GGATG 4 cut(s) 427, 560, 805, 1047
BtsIMutI CAGTG 2 cut(s) 396, 1225
BveI ACCTGC 1 cut(s) 928
Cac8I GCNNGC 4 cut(s) 467, 1062, 1147, 1237
CciI TCATGA 1 cut(s) 1131
Cfr10I RCCGGY 3 cut(s) 104, 1037, 1226
Cfr13I GGNCC 1 cut(s) 996
Csp6I GTAC 2 cut(s) 86, 358
CspAI ACCGGT 1 cut(s) 1226
CviAII CATG 4 cut(s) 646, 922, 1091, 1132
CviQI GTAC 2 cut(s) 86, 358
DdeI CTNAG 4 cut(s) 598, 692, 831, 980
DpnI GATC 2 cut(s) 155, 1136
DpnII GATC 2 cut(s) 153, 1134
EaeI YGGCCR 3 cut(s) 814, 1035, 1087
EagI CGGCCG 2 cut(s) 814, 1035
Eam1104I CTCTTC 2 cut(s) 224, 309
EarI CTCTTC 2 cut(s) 224, 309
EclXI CGGCCG 2 cut(s) 814, 1035
Eco130I CCWWGG 4 cut(s) 165, 670, 999, 1114
Eco31I GGTCTC 1 cut(s) 950
Eco52I CGGCCG 2 cut(s) 814, 1035
Eco57I CTGAAG 1 cut(s) 360
Eco88I CYCGRG 1 cut(s) 447
EcoRII CCWGG 2 cut(s) 929, 1050
EcoT14I CCWWGG 4 cut(s) 165, 670, 999, 1114
ErhI CCWWGG 4 cut(s) 165, 670, 999, 1114
FaeI CATG 4 cut(s) 649, 925, 1094, 1135
FalI AAGNNNNNCTT 4 cut(s) 315, 347, 1179, 1211
FaqI GGGAC 1 cut(s) 97
FatI CATG 4 cut(s) 645, 921, 1090, 1131
FauI CCCGC 1 cut(s) 1168
FauNDI CATATG 1 cut(s) 134
FblI GTMKAC 2 cut(s) 39, 524
Fnu4HI GCNGC 6 cut(s) 72, 747, 814, 1065, 1252, 1266
FokI GGATG 4 cut(s) 434, 547, 812, 1034
Fsp4HI GCNGC 6 cut(s) 72, 747, 814, 1065, 1252, 1266
FspBI CTAG 3 cut(s) 590, 839, 848
GluI GCNGC 6 cut(s) 72, 747, 814, 1065, 1252, 1266
GsuI CTGGAG 1 cut(s) 929
HaeIII GGCC 6 cut(s) 749, 816, 998, 1004, 1037, 1089
HapII CCGG 4 cut(s) 105, 878, 1038, 1227
Hin1II CATG 4 cut(s) 649, 925, 1094, 1135
HincII GTYRAC 1 cut(s) 1075
HindII GTYRAC 1 cut(s) 1075
HindIII AAGCTT 1 cut(s) 623
HinfI GANTC 5 cut(s) 257, 328, 677, 1076, 1140
HpaII CCGG 4 cut(s) 105, 878, 1038, 1227
HphI GGTGA 3 cut(s) 565, 686, 1241
Hpy166II GTNNAC 4 cut(s) 40, 525, 612, 1075
Hpy188I TCNGA 4 cut(s) 262, 981, 1044, 1139
Hpy188III TCNNGA 4 cut(s) 64, 386, 681, 1132
Hpy8I GTNNAC 4 cut(s) 40, 525, 612, 1075
Hpy99I CGWCG 2 cut(s) 541, 1027
HpyAV CCTTC 2 cut(s) 760, 872
HpyCH4III ACNGT 4 cut(s) 90, 406, 532, 1027
HpyCH4IV ACGT 1 cut(s) 536
HpyCH4V TGCA 7 cut(s) 56, 138, 244, 338, 645, 698, 1223
HpyF10VI GCNNNNNNNGC 3 cut(s) 695, 827, 1196
HpyF3I CTNAG 4 cut(s) 598, 692, 831, 980
HpySE526I ACGT 1 cut(s) 536
Hsp92II CATG 4 cut(s) 649, 925, 1094, 1135
Kzo9I GATC 2 cut(s) 153, 1134
LmnI GCTCC 5 cut(s) 262, 910, 934, 1099, 1118
Lsp1109I GCAGC 3 cut(s) 58, 1252, 1263
LweI GCATC 4 cut(s) 65, 91, 298, 1156
MaeI CTAG 3 cut(s) 590, 839, 848
MaeII ACGT 1 cut(s) 536
MaeIII GTNAC 2 cut(s) 532, 674
MalI GATC 2 cut(s) 155, 1136
MboI GATC 2 cut(s) 153, 1134
MboII GAAGA 5 cut(s) 241, 326, 629, 866, 1001
MfeI CAATTG 2 cut(s) 699, 1068
MlsI TGGCCA 1 cut(s) 1089
MluCI AATT 8 cut(s) 139, 196, 508, 660, 699, 785, 1068, 1240
MluNI TGGCCA 1 cut(s) 1089
MlyI GAGTC 2 cut(s) 671, 1070
MmeI TCCRAC 4 cut(s) 349, 416, 882, 948
Mox20I TGGCCA 1 cut(s) 1089
MscI TGGCCA 1 cut(s) 1089
MseI TTAA 3 cut(s) 551, 621, 1173
MslI CAYNNNNRTG 1 cut(s) 133
Msp20I TGGCCA 1 cut(s) 1089
MspA1I CMGCKG 4 cut(s) 499, 1060, 1163, 1265
MspCI CTTAAG 1 cut(s) 550
MspI CCGG 4 cut(s) 105, 878, 1038, 1227
MspR9I CCNGG 2 cut(s) 931, 1052
MunI CAATTG 2 cut(s) 699, 1068
MvaI CCWGG 2 cut(s) 931, 1052
MwoI GCNNNNNNNGC 3 cut(s) 695, 827, 1196
NdeI CATATG 1 cut(s) 134
NdeII GATC 2 cut(s) 153, 1134
NlaIII CATG 4 cut(s) 649, 925, 1094, 1135
NlaIV GGNNCC 3 cut(s) 912, 1056, 1120
NmeAIII GCCGAG 2 cut(s) 699, 722
NmuCI GTSAC 1 cut(s) 674
NspI RCATGY 1 cut(s) 925
PaeR7I CTCGAG 1 cut(s) 447
PagI TCATGA 1 cut(s) 1131
PcsI WCGNNNNNNNCGW 2 cut(s) 536, 948
PfeI GAWTC 3 cut(s) 257, 328, 1140
PinAI ACCGGT 1 cut(s) 1226
PkrI GCNGC 6 cut(s) 73, 748, 815, 1066, 1253, 1267
PleI GAGTC 2 cut(s) 671, 1070
PpsI GAGTC 2 cut(s) 671, 1070
Psp6I CCWGG 2 cut(s) 929, 1050
PspGI CCWGG 2 cut(s) 929, 1050
PspN4I GGNNCC 3 cut(s) 912, 1056, 1120
PspPI GGNCC 1 cut(s) 996
PspXI VCTCGAGB 1 cut(s) 447
PvuII CAGCTG 2 cut(s) 499, 1265
RsaI GTAC 2 cut(s) 87, 359
RsaNI GTAC 2 cut(s) 86, 358
RseI CAYNNNNRTG 1 cut(s) 133
SaqAI TTAA 3 cut(s) 551, 621, 1173
SatI GCNGC 6 cut(s) 72, 747, 814, 1065, 1252, 1266
Sau3AI GATC 2 cut(s) 153, 1134
Sau96I GGNCC 1 cut(s) 996
SchI GAGTC 2 cut(s) 671, 1070
ScrFI CCNGG 2 cut(s) 931, 1052
SfaNI GCATC 4 cut(s) 65, 91, 298, 1156
Sfr274I CTCGAG 1 cut(s) 447
SlaI CTCGAG 1 cut(s) 447
SmiMI CAYNNNNRTG 1 cut(s) 133
SmlI CTYRAG 4 cut(s) 278, 384, 447, 550
SmoI CTYRAG 4 cut(s) 278, 384, 447, 550
Sse9I AATT 8 cut(s) 139, 196, 508, 660, 699, 785, 1068, 1240
SsiI CCGC 8 cut(s) 181, 311, 746, 813, 1058, 1064, 1127, 1161
SspMI CTAG 3 cut(s) 590, 839, 848
StyD4I CCNGG 2 cut(s) 929, 1050
StyI CCWWGG 4 cut(s) 165, 670, 999, 1114
TaaI ACNGT 4 cut(s) 90, 406, 532, 1027
TaiI ACGT 1 cut(s) 539
TaqI TCGA 2 cut(s) 448, 539
TaqII GACCGA 1 cut(s) 932
TasI AATT 8 cut(s) 139, 196, 508, 660, 699, 785, 1068, 1240
TatI WGTACW 1 cut(s) 85
TauI GCSGC 3 cut(s) 749, 816, 1067
TfiI GAWTC 3 cut(s) 257, 328, 1140
Tru1I TTAA 3 cut(s) 551, 621, 1173
Tru9I TTAA 3 cut(s) 551, 621, 1173
TscAI CASTG 2 cut(s) 403, 1225
TseFI GTSAC 1 cut(s) 674
TseI GCWGC 3 cut(s) 71, 1251, 1265
Tsp45I GTSAC 1 cut(s) 674
TspDTI ATGAA 7 cut(s) 17, 438, 549, 819, 884, 1233, 1263
TspGWI ACGGA 1 cut(s) 306
TspRI CASTG 2 cut(s) 403, 1225
Vha464I CTTAAG 1 cut(s) 550
XceI RCATGY 1 cut(s) 925
XhoI CTCGAG 1 cut(s) 447
XmiI GTMKAC 2 cut(s) 39, 524
XspI CTAG 3 cut(s) 590, 839, 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.