Rroxscaffold_7G00183880

Thiamine-repressible mitochondrial transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005884
Physical Location & Seq
Forward (+)
23058662 .. 23062555
3894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00183880.1

Sequence Viewer

Length: 639 bp
ATGGGAGGAACGATAATTGTCAGCCTCGGTGACTCACGAACTACTCTAAGTGCAATTGCTTCAAACCCTCTCCTGGGAGACATACTTGCTCTTGTCTCAGCAGCTTTGTATTCTGTGTATATTACCCTCATTCGCAAGAAGTTACCTGATGAGGATGATGAGAAAAGTGGTCGTGCTAGTATGGCTCAGTTTCTGGGATTTTTAGGGCTTTCTAACCTTCTTATATTTCTTCCAGTTGCCCTTGTACTGCATTTCTCAAAGTTGGAACCCTTTTATATGCTGACCTGGAAGCAGCTCGGTCTCATTGTTGGTAAAGGTTTGCTGGATAACGTTCTGAGCGATTACTTATGGGCCAAGGCTGTTCTTCTAACAACAACAACAGTAGCAACAGCTGGTCTATCAATACAAGTTCCGTTGGCAGCAATTGTAGACTCTGTGACTGGCCATGCTCCTCATTTTGCTGATTACCTTGGAGCTGTGGCCGTTATGATTGGATTTGTTGGCATTAATATTCCTTCTGATGCCTTTAAGAGGTCGAAAGGAGCTACACTAGAATTAGAGAATGGAAATAATAGAAATAGTAGTTCAATTAGTGAACCTGGTAGTTCATCAGGTCCAGATTCAACTGCCCATTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

212

Amino Acids

22.28

Weight (kDa)

5.74

Isoelectric Point (pI)

27.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 25 - 170 1.2e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 429
AclI AACGTT 1 cut(s) 330
AcoI YGGCCR 2 cut(s) 442, 480
AfaI GTAC 1 cut(s) 246
AfiI CCNNNNNNNGG 3 cut(s) 73, 74, 531
AgsI TTSAA 3 cut(s) 63, 588, 624
AjnI CCWGG 3 cut(s) 72, 284, 598
AluBI AGCT 5 cut(s) 104, 295, 392, 476, 545
AluI AGCT 5 cut(s) 104, 295, 392, 476, 545
Alw26I GTCTC 3 cut(s) 72, 100, 305
AlwNI CAGNNNCTG 1 cut(s) 193
AoxI GGCC 3 cut(s) 351, 442, 480
ApeKI GCWGC 3 cut(s) 101, 292, 419
AseI ATTAAT 1 cut(s) 507
AspS9I GGNCC 2 cut(s) 351, 614
AsuHPI GGTGA 1 cut(s) 41
AvaII GGWCC 1 cut(s) 614
BalI TGGCCA 1 cut(s) 444
BbvI GCAGC 3 cut(s) 113, 304, 431
BceAI ACGGC 1 cut(s) 467
BciT130I CCWGG 3 cut(s) 74, 286, 600
BcoDI GTCTC 3 cut(s) 72, 100, 305
BfaI CTAG 2 cut(s) 177, 551
BisI GCNGC 3 cut(s) 102, 293, 420
BlsI GCNGC 3 cut(s) 103, 294, 421
Bme1390I CCNGG 3 cut(s) 74, 286, 600
Bme18I GGWCC 1 cut(s) 614
BmgT120I GGNCC 2 cut(s) 351, 614
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 3 cut(s) 74, 286, 600
BmsI GCATC 1 cut(s) 511
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 4 cut(s) 25, 73, 354, 469
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 74, 531
Bse1I ACTGG 2 cut(s) 233, 445
BseBI CCWGG 3 cut(s) 74, 286, 600
BseDI CCNNGG 4 cut(s) 25, 73, 354, 469
BseGI GGATG 1 cut(s) 160
BseLI CCNNNNNNNGG 3 cut(s) 73, 74, 531
BseMII CTCAG 3 cut(s) 111, 200, 326
BseNI ACTGG 2 cut(s) 233, 445
BseRI GAGGAG 1 cut(s) 441
BseXI GCAGC 3 cut(s) 113, 304, 431
BshFI GGCC 3 cut(s) 353, 444, 482
BslI CCNNNNNNNGG 3 cut(s) 73, 74, 531
BsmAI GTCTC 3 cut(s) 72, 100, 305
BsnI GGCC 3 cut(s) 353, 444, 482
Bso31I GGTCTC 1 cut(s) 305
BspANI GGCC 3 cut(s) 353, 444, 482
BspCNI CTCAG 3 cut(s) 110, 199, 327
BspLI GGNNCC 1 cut(s) 267
BspTNI GGTCTC 1 cut(s) 305
BsrI ACTGG 2 cut(s) 233, 445
BssECI CCNNGG 4 cut(s) 25, 73, 354, 469
BssT1I CCWWGG 2 cut(s) 354, 469
Bst2UI CCWGG 3 cut(s) 74, 286, 600
Bst4CI ACNGT 1 cut(s) 382
BstDEI CTNAG 4 cut(s) 47, 97, 186, 335
BstENI CCTNNNNNAGG 1 cut(s) 529
BstF5I GGATG 1 cut(s) 160
BstMAI GTCTC 3 cut(s) 72, 100, 305
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstNI CCWGG 3 cut(s) 74, 286, 600
BstSCI CCNGG 3 cut(s) 72, 284, 598
BstV1I GCAGC 3 cut(s) 113, 304, 431
BsuRI GGCC 3 cut(s) 353, 444, 482
BtsCI GGATG 1 cut(s) 160
CaiI CAGNNNCTG 1 cut(s) 193
Cfr13I GGNCC 2 cut(s) 351, 614
CsiI ACCWGGT 1 cut(s) 598
Csp6I GTAC 1 cut(s) 245
CviAII CATG 1 cut(s) 446
CviQI GTAC 1 cut(s) 245
DdeI CTNAG 4 cut(s) 47, 97, 186, 335
EaeI YGGCCR 2 cut(s) 442, 480
Eco130I CCWWGG 2 cut(s) 354, 469
Eco31I GGTCTC 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 614
EcoNI CCTNNNNNAGG 1 cut(s) 529
EcoRII CCWGG 3 cut(s) 72, 284, 598
EcoT14I CCWWGG 2 cut(s) 354, 469
ErhI CCWWGG 2 cut(s) 354, 469
FaeI CATG 1 cut(s) 449
FatI CATG 1 cut(s) 445
FblI GTMKAC 1 cut(s) 429
Fnu4HI GCNGC 3 cut(s) 102, 293, 420
FokI GGATG 1 cut(s) 167
Fsp4HI GCNGC 3 cut(s) 102, 293, 420
FspBI CTAG 2 cut(s) 177, 551
GluI GCNGC 3 cut(s) 102, 293, 420
HaeIII GGCC 3 cut(s) 353, 444, 482
Hin1II CATG 1 cut(s) 449
HinfI GANTC 3 cut(s) 32, 431, 620
HphI GGTGA 1 cut(s) 41
Hpy166II GTNNAC 2 cut(s) 430, 596
Hpy188I TCNGA 2 cut(s) 336, 520
Hpy188III TCNNGA 2 cut(s) 36, 617
Hpy8I GTNNAC 2 cut(s) 430, 596
HpyAV CCTTC 2 cut(s) 227, 525
HpyCH4III ACNGT 1 cut(s) 382
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 2 cut(s) 53, 250
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HpyF3I CTNAG 4 cut(s) 47, 97, 186, 335
HpySE526I ACGT 1 cut(s) 330
Hsp92II CATG 1 cut(s) 449
LmnI GCTCC 3 cut(s) 454, 473, 542
Lsp1109I GCAGC 3 cut(s) 113, 304, 431
LweI GCATC 1 cut(s) 511
MabI ACCWGGT 1 cut(s) 598
MaeI CTAG 2 cut(s) 177, 551
MaeII ACGT 1 cut(s) 330
MaeIII GTNAC 3 cut(s) 29, 141, 436
MboII GAAGA 2 cut(s) 221, 356
MfeI CAATTG 2 cut(s) 54, 423
MlsI TGGCCA 1 cut(s) 444
MluCI AATT 5 cut(s) 15, 54, 423, 554, 588
MluNI TGGCCA 1 cut(s) 444
MlyI GAGTC 2 cut(s) 26, 425
MmeI TCCRAC 1 cut(s) 243
MnlI CCTC 6 cut(s) 35, 78, 137, 145, 462, 525
Mox20I TGGCCA 1 cut(s) 444
MscI TGGCCA 1 cut(s) 444
MseI TTAA 2 cut(s) 507, 528
Msp20I TGGCCA 1 cut(s) 444
MspA1I CMGCKG 1 cut(s) 392
MspR9I CCNGG 3 cut(s) 74, 286, 600
MunI CAATTG 2 cut(s) 54, 423
MvaI CCWGG 3 cut(s) 74, 286, 600
MwoI GCNNNNNNNGC 1 cut(s) 182
NlaIII CATG 1 cut(s) 449
NlaIV GGNNCC 1 cut(s) 267
NmuCI GTSAC 2 cut(s) 29, 436
PcsI WCGNNNNNNNCGW 1 cut(s) 336
PfeI GAWTC 1 cut(s) 620
PkrI GCNGC 3 cut(s) 103, 294, 421
PleI GAGTC 2 cut(s) 26, 425
PpsI GAGTC 2 cut(s) 26, 425
PshBI ATTAAT 1 cut(s) 507
Psp1406I AACGTT 1 cut(s) 330
Psp6I CCWGG 3 cut(s) 72, 284, 598
PspGI CCWGG 3 cut(s) 72, 284, 598
PspN4I GGNNCC 1 cut(s) 267
PspPI GGNCC 2 cut(s) 351, 614
PstNI CAGNNNCTG 1 cut(s) 193
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 1 cut(s) 246
RsaNI GTAC 1 cut(s) 245
SaqAI TTAA 2 cut(s) 507, 528
SatI GCNGC 3 cut(s) 102, 293, 420
Sau96I GGNCC 2 cut(s) 351, 614
SchI GAGTC 2 cut(s) 26, 425
ScrFI CCNGG 3 cut(s) 74, 286, 600
SexAI ACCWGGT 1 cut(s) 598
SfaNI GCATC 1 cut(s) 511
SinI GGWCC 1 cut(s) 614
Sse9I AATT 5 cut(s) 15, 54, 423, 554, 588
SspI AATATT 1 cut(s) 511
SspMI CTAG 2 cut(s) 177, 551
StyD4I CCNGG 3 cut(s) 72, 284, 598
StyI CCWWGG 2 cut(s) 354, 469
TaaI ACNGT 1 cut(s) 382
TaiI ACGT 1 cut(s) 333
TaqI TCGA 1 cut(s) 536
TaqII GACCGA 1 cut(s) 287
TasI AATT 5 cut(s) 15, 54, 423, 554, 588
TatI WGTACW 1 cut(s) 244
TfiI GAWTC 1 cut(s) 620
Tru1I TTAA 2 cut(s) 507, 528
Tru9I TTAA 2 cut(s) 507, 528
TseFI GTSAC 2 cut(s) 29, 436
TseI GCWGC 3 cut(s) 101, 292, 419
Tsp45I GTSAC 2 cut(s) 29, 436
TspDTI ATGAA 2 cut(s) 597, 624
TspGWI ACGGA 1 cut(s) 402
VpaK11BI GGWCC 1 cut(s) 614
VspI ATTAAT 1 cut(s) 507
XagI CCTNNNNNAGG 1 cut(s) 529
XmiI GTMKAC 1 cut(s) 429
XspI CTAG 2 cut(s) 177, 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.