MD05G1176600.v1.1

Thiamine-repressible mitochondrial transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005884
Physical Location & Seq
Reverse (-)
30405072 .. 30407572
2501 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1176600.v1.1.491

Sequence Viewer

Length: 1284 bp
ATGAAAACTGAAGTTTGGAAGTGGGTTTTGGGTTTGATATACATATTTGCTGTTGCATCCATCTGGATAGCTGCCAGTTTCGTAGTGCAGTCGGTTGTAGATGCCGGTGTGTCCCCGTTCCTCATCACATACATATGCAATTCTCTGTTTGTGATCTACATTCCCTTGGTTGAAATCGGGCGGTATTTGGAAGATAATTTTGAGGGGTTTTGGATTTGGAGAAGTAGGAAGAGTAGCCCTTTGCAAAGGTTAGGAGAGTCGGAGCAAATCACTCTCCTTGAGGAGACTGATGGAGTTGTGATAGCGGATGCGGAAGAGGGGGAAGTTAATCTTGGTGTAGAACCGAAAGTTGGATCATACGAGCTTGTTGGGATTTCAGCAACTCAAGACAATGTCACTGAAACGGTAGATAAGCAAGTGGATGGAAAAGGGCGCTGGACACGAACTCGAGTGGCTAAAGTTAGCCTACTGATTTCCCCGTTTTGGTTTTTTGCACAGCTGACTTTCAATTTGTCATTGAAGTATACTACCGTTACATCGAATACCATCTTAAGCAGTTCATCCAGCCTTTTCACCTTTTTGGTCTCCCTAGTATTCTTAGGGGAGAAGTTTACTTTGGTTAAGCTTATTAGCGTTCTTCTTTGCATGGGAGGAACGATAATTGTCAGCCTTGGTGACTCACGAACTGCTCTAAGTGCAATTGCTTCAAACCCTCTCCTCGGAGACATACTTGCTCTTGTCTCGGCGGCCTTATATTCTGTCTATATTACCCTCATTCGCAAGAAATTACCTGATGAAGATGATGAAAAAAGCGGCCGTGCCAGTATGGCTCAGTTTCTAGGATTTCTAGGGCTTTCCAACCTTCTTATTTTTCTTCCGGTTGCCCTTATACTTCATTTCTCAAAGCTAGAGCCGTTTTATATGCTTACATGGGAGCAGGTTGGTCTCATCGTTGGTAAAGGTTTGCTGGATAATGTTCTGAGTGACTACTTATGGGCCAAGGCCGTTCTTCTAACAACCACCACGGTAGCAACAGCCGGTCTGACGATCCAGGTTCCATTGGCGGCAATTGTGGACTCGATGACTGGCCATGCTCCTCATTTTGCTGATTATCTTGGAGCAGGGGCTGTCATGATCGGATTCGCTGGCATAAACATTCCTTCTGATGCTTTTAACAGACCAAAAGGAGCTGTACTTGTGTTAGAGAACGAAAATCTCAGCGAGACCAGTGAAGGTCGCAATGCATCGGGCACGGGAAGGCCGGTTTCAGCTGCCCCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

428

Amino Acids

46.28

Weight (kDa)

4.96

Isoelectric Point (pI)

30.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 143 - 261 1.4e-07 Purine nucleobase transmembrane transport
EamA PF00892 154 - 223 9e-10 EamA-like transporter family
TPT PF03151 160 - 305 2.8e-07 Triose-phosphate Transporter family
SLC35F PF06027 173 - 258 6.8e-10 Solute carrier family 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 928
AccI GTMKAC 1 cut(s) 524
AciI CCGC 6 cut(s) 181, 305, 311, 746, 813, 1064
AclWI GGATC 2 cut(s) 361, 1042
AcoI YGGCCR 2 cut(s) 814, 1087
AcuI CTGAAG 1 cut(s) 30
AfaI GTAC 1 cut(s) 1194
AfiI CCNNNNNNNGG 3 cut(s) 350, 483, 719
AflII CTTAAG 1 cut(s) 550
AgsI TTSAA 4 cut(s) 173, 508, 520, 708
AjnI CCWGG 1 cut(s) 1050
AjuI GAANNNNNNNTTGG 8 cut(s) 11, 43, 315, 333, 347, 365, 599, 631
AluBI AGCT 7 cut(s) 71, 364, 499, 625, 907, 1190, 1271
AluI AGCT 7 cut(s) 71, 364, 499, 625, 907, 1190, 1271
Alw26I GTCTC 6 cut(s) 278, 589, 717, 745, 950, 1217
AlwI GGATC 2 cut(s) 361, 1042
AlwNI CAGNNNCTG 1 cut(s) 1127
Ama87I CYCGRG 1 cut(s) 447
AoxI GGCC 6 cut(s) 747, 814, 996, 1002, 1087, 1259
ApeKI GCWGC 2 cut(s) 71, 1271
ArsI GACNNNNNNTTYG 2 cut(s) 493, 525
AspLEI GCGC 1 cut(s) 435
AspS9I GGNCC 1 cut(s) 996
AsuHPI GGTGA 2 cut(s) 565, 686
AvaI CYCGRG 1 cut(s) 447
BaeGI GKGCMC 1 cut(s) 1253
BalI TGGCCA 1 cut(s) 1089
BbvI GCAGC 2 cut(s) 58, 1258
BccI CCATC 4 cut(s) 68, 284, 416, 554
BceAI ACGGC 3 cut(s) 801, 898, 989
BciT130I CCWGG 1 cut(s) 1052
BcoDI GTCTC 6 cut(s) 278, 589, 717, 745, 950, 1217
BfaI CTAG 4 cut(s) 590, 839, 848, 908
BfoI RGCGCY 1 cut(s) 436
BfrI CTTAAG 1 cut(s) 550
BfuAI ACCTGC 1 cut(s) 928
BglI GCCNNNNNGGC 1 cut(s) 827
BisI GCNGC 5 cut(s) 72, 747, 814, 1065, 1272
BlsI GCNGC 5 cut(s) 73, 748, 815, 1066, 1273
Bme1390I CCNGG 1 cut(s) 1052
BmeT110I CYCGRG 1 cut(s) 447
BmgT120I GGNCC 1 cut(s) 996
BmiI GGNNCC 1 cut(s) 1056
BmrFI CCNGG 1 cut(s) 1052
BmsI GCATC 5 cut(s) 65, 91, 298, 1156, 1253
BpuEI CTTGAG 2 cut(s) 299, 369
BsaI GGTCTC 3 cut(s) 589, 950, 1217
BsaJI CCNNGG 5 cut(s) 165, 670, 718, 999, 1023
BsaWI WCCGGW 1 cut(s) 877
Bsc4I CCNNNNNNNGG 3 cut(s) 350, 483, 719
Bse118I RCCGGY 3 cut(s) 104, 1037, 1261
Bse1I ACTGG 4 cut(s) 75, 822, 1090, 1227
Bse3DI GCAATG 1 cut(s) 1246
BseBI CCWGG 1 cut(s) 1052
BseDI CCNNGG 5 cut(s) 165, 670, 718, 999, 1023
BseGI GGATG 4 cut(s) 56, 313, 427, 560
BseLI CCNNNNNNNGG 3 cut(s) 350, 483, 719
BseMI GCAATG 1 cut(s) 1246
BseMII CTCAG 3 cut(s) 845, 971, 1231
BseNI ACTGG 4 cut(s) 75, 822, 1090, 1227
BseRI GAGGAG 3 cut(s) 296, 707, 1086
BseSI GKGCMC 1 cut(s) 1253
BseX3I CGGCCG 1 cut(s) 814
BseXI GCAGC 2 cut(s) 58, 1258
BsgI GTGCAG 1 cut(s) 107
Bsh1285I CGRYCG 1 cut(s) 817
BshFI GGCC 6 cut(s) 749, 816, 998, 1004, 1089, 1261
BsiEI CGRYCG 1 cut(s) 817
BsiHKCI CYCGRG 1 cut(s) 447
BsiSI CCGG 4 cut(s) 105, 878, 1038, 1262
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 3 cut(s) 350, 483, 719
BsmAI GTCTC 6 cut(s) 278, 589, 717, 745, 950, 1217
BsmFI GGGAC 1 cut(s) 97
BsnI GGCC 6 cut(s) 749, 816, 998, 1004, 1089, 1261
Bso31I GGTCTC 3 cut(s) 589, 950, 1217
BsoBI CYCGRG 1 cut(s) 447
Bsp1286I GDGCHC 1 cut(s) 1253
Bsp143I GATC 4 cut(s) 153, 353, 1047, 1134
BspACI CCGC 6 cut(s) 181, 305, 311, 746, 813, 1064
BspANI GGCC 6 cut(s) 749, 816, 998, 1004, 1089, 1261
BspCNI CTCAG 3 cut(s) 844, 972, 1230
BspHI TCATGA 1 cut(s) 1131
BspLI GGNNCC 1 cut(s) 1056
BspMI ACCTGC 1 cut(s) 928
BspPI GGATC 2 cut(s) 361, 1042
BspTI CTTAAG 1 cut(s) 550
BspTNI GGTCTC 3 cut(s) 589, 950, 1217
BsrDI GCAATG 1 cut(s) 1246
BsrFI RCCGGY 3 cut(s) 104, 1037, 1261
BsrI ACTGG 4 cut(s) 75, 822, 1090, 1227
BssAI RCCGGY 3 cut(s) 104, 1037, 1261
BssECI CCNNGG 5 cut(s) 165, 670, 718, 999, 1023
BssMI GATC 4 cut(s) 153, 353, 1047, 1134
BssNAI GTATAC 1 cut(s) 525
BssT1I CCWWGG 3 cut(s) 165, 670, 999
Bst1107I GTATAC 1 cut(s) 525
Bst2UI CCWGG 1 cut(s) 1052
Bst4CI ACNGT 3 cut(s) 406, 532, 1027
Bst6I CTCTTC 2 cut(s) 224, 309
BstAFI CTTAAG 1 cut(s) 550
BstC8I GCNNGC 1 cut(s) 1147
BstDEI CTNAG 5 cut(s) 598, 692, 831, 980, 1217
BstDSI CCRYGG 1 cut(s) 1023
BstF5I GGATG 4 cut(s) 56, 313, 427, 560
BstH2I RGCGCY 1 cut(s) 436
BstHHI GCGC 1 cut(s) 435
BstKTI GATC 4 cut(s) 156, 356, 1050, 1137
BstMAI GTCTC 6 cut(s) 278, 589, 717, 745, 950, 1217
BstMBI GATC 4 cut(s) 153, 353, 1047, 1134
BstMCI CGRYCG 1 cut(s) 817
BstMWI GCNNNNNNNGC 2 cut(s) 695, 827
BstNI CCWGG 1 cut(s) 1052
BstSCI CCNGG 1 cut(s) 1050
BstSLI GKGCMC 1 cut(s) 1253
BstV1I GCAGC 2 cut(s) 58, 1258
BstZ17I GTATAC 1 cut(s) 525
BstZI CGGCCG 1 cut(s) 814
BsuRI GGCC 6 cut(s) 749, 816, 998, 1004, 1089, 1261
BtgI CCRYGG 1 cut(s) 1023
BtsCI GGATG 4 cut(s) 56, 313, 427, 560
BtsIMutI CAGTG 2 cut(s) 396, 1234
BveI ACCTGC 1 cut(s) 928
Cac8I GCNNGC 1 cut(s) 1147
CaiI CAGNNNCTG 1 cut(s) 1127
CciI TCATGA 1 cut(s) 1131
CfoI GCGC 1 cut(s) 435
Cfr10I RCCGGY 3 cut(s) 104, 1037, 1261
Cfr13I GGNCC 1 cut(s) 996
Csp6I GTAC 1 cut(s) 1193
CviAII CATG 4 cut(s) 646, 930, 1091, 1132
CviQI GTAC 1 cut(s) 1193
DdeI CTNAG 5 cut(s) 598, 692, 831, 980, 1217
DpnI GATC 4 cut(s) 155, 355, 1049, 1136
DpnII GATC 4 cut(s) 153, 353, 1047, 1134
EaeI YGGCCR 2 cut(s) 814, 1087
EagI CGGCCG 1 cut(s) 814
Eam1104I CTCTTC 2 cut(s) 224, 309
EarI CTCTTC 2 cut(s) 224, 309
EclXI CGGCCG 1 cut(s) 814
Eco130I CCWWGG 3 cut(s) 165, 670, 999
Eco31I GGTCTC 3 cut(s) 589, 950, 1217
Eco52I CGGCCG 1 cut(s) 814
Eco57I CTGAAG 1 cut(s) 30
Eco88I CYCGRG 1 cut(s) 447
EcoRII CCWGG 1 cut(s) 1050
EcoT14I CCWWGG 3 cut(s) 165, 670, 999
EcoT22I ATGCAT 1 cut(s) 1246
ErhI CCWWGG 3 cut(s) 165, 670, 999
FaeI CATG 4 cut(s) 649, 933, 1094, 1135
FalI AAGNNNNNCTT 2 cut(s) 315, 347
FaqI GGGAC 1 cut(s) 97
FatI CATG 4 cut(s) 645, 929, 1090, 1131
FauNDI CATATG 1 cut(s) 134
FblI GTMKAC 1 cut(s) 524
Fnu4HI GCNGC 5 cut(s) 72, 747, 814, 1065, 1272
FokI GGATG 4 cut(s) 43, 320, 434, 547
Fsp4HI GCNGC 5 cut(s) 72, 747, 814, 1065, 1272
FspBI CTAG 4 cut(s) 590, 839, 848, 908
GlaI GCGC 1 cut(s) 434
GluI GCNGC 5 cut(s) 72, 747, 814, 1065, 1272
HaeII RGCGCY 1 cut(s) 436
HaeIII GGCC 6 cut(s) 749, 816, 998, 1004, 1089, 1261
HapII CCGG 4 cut(s) 105, 878, 1038, 1262
HhaI GCGC 1 cut(s) 435
Hin1II CATG 4 cut(s) 649, 933, 1094, 1135
Hin6I GCGC 1 cut(s) 433
HinP1I GCGC 1 cut(s) 433
HindIII AAGCTT 1 cut(s) 623
HinfI GANTC 4 cut(s) 257, 677, 1076, 1140
HpaII CCGG 4 cut(s) 105, 878, 1038, 1262
HphI GGTGA 2 cut(s) 565, 686
Hpy166II GTNNAC 3 cut(s) 525, 612, 1075
Hpy188I TCNGA 6 cut(s) 262, 722, 981, 1044, 1139, 1165
Hpy188III TCNNGA 4 cut(s) 64, 386, 681, 1132
Hpy8I GTNNAC 3 cut(s) 525, 612, 1075
HpyAV CCTTC 4 cut(s) 872, 1170, 1226, 1251
HpyCH4III ACNGT 3 cut(s) 406, 532, 1027
HpyCH4V TGCA 8 cut(s) 56, 88, 138, 244, 494, 645, 698, 1244
HpyF10VI GCNNNNNNNGC 2 cut(s) 695, 827
HpyF3I CTNAG 5 cut(s) 598, 692, 831, 980, 1217
Hsp92II CATG 4 cut(s) 649, 933, 1094, 1135
HspAI GCGC 1 cut(s) 433
Kzo9I GATC 4 cut(s) 153, 353, 1047, 1134
LmnI GCTCC 5 cut(s) 262, 934, 1099, 1118, 1187
Lsp1109I GCAGC 2 cut(s) 58, 1258
LweI GCATC 5 cut(s) 65, 91, 298, 1156, 1253
MaeI CTAG 4 cut(s) 590, 839, 848, 908
MaeIII GTNAC 4 cut(s) 394, 532, 674, 983
MalI GATC 4 cut(s) 155, 355, 1049, 1136
MboI GATC 4 cut(s) 153, 353, 1047, 1134
MboII GAAGA 7 cut(s) 203, 241, 326, 629, 809, 866, 1001
MfeI CAATTG 2 cut(s) 699, 1068
MhlI GDGCHC 1 cut(s) 1253
MlsI TGGCCA 1 cut(s) 1089
MluCI AATT 7 cut(s) 139, 196, 508, 660, 699, 785, 1068
MluNI TGGCCA 1 cut(s) 1089
MlyI GAGTC 3 cut(s) 266, 671, 1070
MmeI TCCRAC 3 cut(s) 240, 331, 882
MnlI CCTC 9 cut(s) 131, 196, 274, 310, 644, 723, 728, 782, 1107
Mox20I TGGCCA 1 cut(s) 1089
Mph1103I ATGCAT 1 cut(s) 1246
MscI TGGCCA 1 cut(s) 1089
MseI TTAA 4 cut(s) 327, 551, 621, 1173
MslI CAYNNNNRTG 1 cut(s) 133
Msp20I TGGCCA 1 cut(s) 1089
MspA1I CMGCKG 2 cut(s) 499, 1271
MspCI CTTAAG 1 cut(s) 550
MspI CCGG 4 cut(s) 105, 878, 1038, 1262
MspR9I CCNGG 1 cut(s) 1052
MunI CAATTG 2 cut(s) 699, 1068
MvaI CCWGG 1 cut(s) 1052
MwoI GCNNNNNNNGC 2 cut(s) 695, 827
NdeI CATATG 1 cut(s) 134
NdeII GATC 4 cut(s) 153, 353, 1047, 1134
NlaIII CATG 4 cut(s) 649, 933, 1094, 1135
NlaIV GGNNCC 1 cut(s) 1056
NmeAIII GCCGAG 1 cut(s) 722
NmuCI GTSAC 3 cut(s) 394, 674, 983
NsiI ATGCAT 1 cut(s) 1246
PaeR7I CTCGAG 1 cut(s) 447
PagI TCATGA 1 cut(s) 1131
PfeI GAWTC 1 cut(s) 1140
PflFI GACNNNGTC 1 cut(s) 392
PkrI GCNGC 5 cut(s) 73, 748, 815, 1066, 1273
PleI GAGTC 3 cut(s) 265, 671, 1070
PpsI GAGTC 3 cut(s) 265, 671, 1070
Psp6I CCWGG 1 cut(s) 1050
PspGI CCWGG 1 cut(s) 1050
PspN4I GGNNCC 1 cut(s) 1056
PspPI GGNCC 1 cut(s) 996
PspXI VCTCGAGB 1 cut(s) 447
PstNI CAGNNNCTG 1 cut(s) 1127
PsyI GACNNNGTC 1 cut(s) 392
PvuII CAGCTG 2 cut(s) 499, 1271
RsaI GTAC 1 cut(s) 1194
RsaNI GTAC 1 cut(s) 1193
RseI CAYNNNNRTG 1 cut(s) 133
SaqAI TTAA 4 cut(s) 327, 551, 621, 1173
SatI GCNGC 5 cut(s) 72, 747, 814, 1065, 1272
Sau3AI GATC 4 cut(s) 153, 353, 1047, 1134
Sau96I GGNCC 1 cut(s) 996
SchI GAGTC 3 cut(s) 266, 671, 1070
ScrFI CCNGG 1 cut(s) 1052
SduI GDGCHC 1 cut(s) 1253
SfaNI GCATC 5 cut(s) 65, 91, 298, 1156, 1253
Sfr274I CTCGAG 1 cut(s) 447
SlaI CTCGAG 1 cut(s) 447
SmiMI CAYNNNNRTG 1 cut(s) 133
SmlI CTYRAG 4 cut(s) 278, 384, 447, 550
SmoI CTYRAG 4 cut(s) 278, 384, 447, 550
Sse9I AATT 7 cut(s) 139, 196, 508, 660, 699, 785, 1068
SsiI CCGC 6 cut(s) 181, 305, 311, 746, 813, 1064
SspMI CTAG 4 cut(s) 590, 839, 848, 908
StyD4I CCNGG 1 cut(s) 1050
StyI CCWWGG 3 cut(s) 165, 670, 999
TaaI ACNGT 3 cut(s) 406, 532, 1027
TaqI TCGA 3 cut(s) 448, 539, 1079
TasI AATT 7 cut(s) 139, 196, 508, 660, 699, 785, 1068
TatI WGTACW 1 cut(s) 1192
TauI GCSGC 3 cut(s) 749, 816, 1067
TfiI GAWTC 1 cut(s) 1140
Tru1I TTAA 4 cut(s) 327, 551, 621, 1173
Tru9I TTAA 4 cut(s) 327, 551, 621, 1173
TscAI CASTG 2 cut(s) 403, 1234
TseFI GTSAC 3 cut(s) 394, 674, 983
TseI GCWGC 2 cut(s) 71, 1271
Tsp45I GTSAC 3 cut(s) 394, 674, 983
TspDTI ATGAA 6 cut(s) 17, 549, 810, 819, 884, 1269
TspRI CASTG 2 cut(s) 403, 1234
Tth111I GACNNNGTC 1 cut(s) 392
Vha464I CTTAAG 1 cut(s) 550
XhoI CTCGAG 1 cut(s) 447
XmiI GTMKAC 1 cut(s) 524
XspI CTAG 4 cut(s) 590, 839, 848, 908
Zsp2I ATGCAT 1 cut(s) 1246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.