FvH4_2g19510

Sterol 3-beta-glucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001484
Physical Location & Seq
Forward (+)
16557362 .. 16565095
7734 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19510.t2

Sequence Viewer

Length: 2109 bp
ATGAACCACCCTCACTGCTCCACCATCCACTTTTATCGAACGAACGTGCCCCCCTTCAGAATCCAATTCCCTCTTTCTTCCTTCTCCTTCTTCTCCGATTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTTTCTGGAATTTCGAATCTCTCAGAATCCAAAGCCACTCTCCTCTTCTCGCTTCGCTTCCGATCTGGATCAGGACCGGCCGCGCGGTCCGGAACAGGCGGCGGTGATGGCGGAGAATCGCCGGAGCAGTTCCGAGAGTTCTGTTGAAGTTTCGCTGGCAATTGAGCGGGAAATTGACGGCGGGAGTGGCCGGATAAGCGTTGAGTTGGAGAACGGACAGGCCAGTGGCGGTGGCGGAGGCGTCAGTGCTCCGGTGACCAACGGTAGCTCTGCAGGGCATTCAGGGAGTTCTGACTTTAGCTTTTCGAAAGCTAATCCAGCGGAGATATCACCAACTAATGAAAAGCCAGTGCAACATTCGGTTCCAGTAAAGTTGGAGAGATCAAAAACGGAGAGCACCAGACCTAGTGTATTGTCCAAAGAGGCAGCAAAGATTTTCGACAACAAAATTCCAGTTCAGCAGAAGTTAAAGCTGTTAAATAGAATAGCTACTGTCAAAGATGACGGAACTGTCGAATTCGATGTTCCAGGGGATATTGAAGCTCAATCTTTTGGTGTTGGAACCATTTCCGGTTCTACTGATGTTGCTGATGAGACTCTTGATGCAACCGACCTTCAATGCATACCACCAATGCAAATAGTAATGCTTATTGTTGGAACGCGTGGAGATGTGCAGCCATTTATTGCAATTGGCAAACGTTTGCAGGACTATGGCCATCGTGTAAGACTAGCTACTCATTCAAATTTTAAAGAGTTTGTCTTGACTGCTGGCTTGGAGTTTTATCCTCTAGGCGGTGATCCAAAGATCCTTGCTGGCTATATGGTTAAAAATAAAGGCTTCTTGCCATCAGGTCCTTCTGAGATTCCTATTCAGCGTAACCAGATAAAGGAAATTATATATTCCTTACTTCCAGCTTGCAAAGACCCTGATCTAGATTCTGGTATCCTCTTTAAAGCGGATGCAATTATTGCAAACCCCCCAGCATACGGGCATACTCATGTGGCGGAAGCACTGAAAATACCTCTTCATATATTTTTTACAATGCCATGGACGCCAACAAGTGAATTTCCACATCCTTTGTCCCGTGTTAAGCAACAGACTGGATATAGGCTTTCGTATCAAATTGTTGACTCCTTAATATGGCTTGGAATACGGGACATGATTAATGATGTTCGGAAGAAAAGATTGAACTTACGACCAGTTACATATTTAAGTGGTTCACAAGGCTCCGAGTCTGATGTCCCACATGGATACATATGGAGTCCCCACCTTGTTCCTAAACCAAAAGATTGGGGACCTAAGGTTGATGTGGTGGGATTTTGCTTCCTTGATCTTGCATCAAATTATCAACCTCCAGAACCTCTAGTGAAATGGCTTGAAGCTGGTGACAAGCCTATATACATTGGATTTGGTAGCCTTCCTGTTCAAGAGCCACAGAAAATGACACAAATAATCGTCGAAGCATTAGAACAGACAGGACAGAGAGGCATCATTAACAAAGGCTGGGGTGGCCTTGGGGATTTGGCTGAGCCAAAGGATTCCATATACTTACTGGACAACTGCCCTCATGACTGGCTGTTTCTTCACTGCAAGGCAGTGGTGCATCATGGAGGTGCTGGAACAACGGCTGCTGGTCTAAAAGCTGCATGTCCGACAACAATAGTTCCTTTCTTTGGAGACCAACCCTTCTGGGGAGAACGAGTACATGCCAGAGGAGTTGGCCCTGCGCCCATTGCAGTTGATGAGTTCTCACTTCCCAAGTTGGTTGCTGCAATAAATTACATGCTTGATCCCAAGGTGAAGGAGCGTGCTATTGAACTTGCAAAGGATATGGAGAATGAGGATGGTGTGACAGGAGCAGTCATGGCCTTCTTTAAGCATCTTCCTCCCAAAAAGCCAGAATCTGAGCCAGAAACCACGTCATCGGGCCTTTTCTCGATAAGTAGATGTTTTGGTTGTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_transf_28 UDPGT

Protein Analysis

703

Amino Acids

76.55

Weight (kDa)

6.12

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_28 PF03033 175 - 318 1.9e-35 Glycosyltransferase family 28 N-terminal domain
UDPGT PF00201 428 - 570 5.6e-06 UDP-glucoronosyl and UDP-glucosyl transferase
EryCIII-like_C PF06722 471 - 575 6.7e-12 Erythromycin biosynthesis protein CIII-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 653
AccBSI CCGCTC 1 cut(s) 310
AccII CGCG 3 cut(s) 226, 228, 805
AccIII TCCGGA 1 cut(s) 232
AclI AACGTT 1 cut(s) 841
AclWI GGATC 4 cut(s) 219, 935, 943, 1928
AcoI YGGCCR 3 cut(s) 221, 331, 856
AcsI RAATTY 5 cut(s) 152, 591, 659, 886, 1208
AcuI CTGAAG 1 cut(s) 40
AcyI GRCGYC 2 cut(s) 384, 1196
AfaI GTAC 1 cut(s) 1848
AfiI CCNNNNNNNGG 6 cut(s) 935, 1030, 1130, 1284, 1817, 1835
AflIII ACRYGT 1 cut(s) 803
AgsI TTSAA 8 cut(s) 290, 683, 761, 885, 1333, 1523, 1571, 1961
AjiI CACGTC 1 cut(s) 2064
AjnI CCWGG 1 cut(s) 670
Alw21I GWGCWC 2 cut(s) 394, 542
Alw26I GTCTC 2 cut(s) 731, 1815
AlwI GGATC 4 cut(s) 219, 935, 943, 1928
AlwNI CAGNNNCTG 1 cut(s) 2048
Aor13HI TCCGGA 1 cut(s) 232
AoxI GGCC 8 cut(s) 221, 331, 363, 856, 1654, 1864, 2010, 2071
ApeKI GCWGC 5 cut(s) 569, 817, 1772, 1787, 1913
ApoI RAATTY 5 cut(s) 152, 591, 659, 886, 1208
AseI ATTAAT 1 cut(s) 1308
Asp700I GAANNNNTTC 1 cut(s) 709
AspLEI GCGC 2 cut(s) 228, 1873
AspS9I GGNCC 6 cut(s) 217, 230, 995, 1439, 1865, 2071
AsuHPI GGTGA 6 cut(s) 259, 409, 465, 950, 1541, 1954
AsuII TTCGAA 2 cut(s) 157, 449
AvaII GGWCC 4 cut(s) 217, 230, 995, 1439
AxyI CCTNAGG 1 cut(s) 1443
BaeGI GKGCMC 1 cut(s) 51
BalI TGGCCA 1 cut(s) 858
Bbv12I GWGCWC 2 cut(s) 394, 542
BbvI GCAGC 5 cut(s) 581, 829, 1759, 1774, 1900
BccI CCATC 5 cut(s) 32, 244, 867, 997, 1982
BceAI ACGGC 2 cut(s) 337, 1785
BciT130I CCWGG 1 cut(s) 672
BciVI GTATCC 2 cut(s) 1097, 1388
BcoDI GTCTC 2 cut(s) 731, 1815
BfaI CTAG 5 cut(s) 549, 872, 932, 1076, 1508
BfmI CTRYAG 1 cut(s) 414
BfuI GTATCC 2 cut(s) 1097, 1388
BisI GCNGC 7 cut(s) 224, 243, 570, 818, 1773, 1788, 1914
BlpI GCTNAGC 1 cut(s) 1671
BlsI GCNGC 7 cut(s) 225, 244, 571, 819, 1774, 1789, 1915
Bme1390I CCNGG 1 cut(s) 672
Bme18I GGWCC 4 cut(s) 217, 230, 995, 1439
BmgBI CACGTC 1 cut(s) 2064
BmgT120I GGNCC 6 cut(s) 217, 230, 995, 1439, 1865, 2071
BmiI GGNNCC 4 cut(s) 507, 706, 1372, 1440
BmrFI CCNGG 1 cut(s) 672
BmsI GCATC 6 cut(s) 736, 1093, 1490, 1641, 1756, 2032
BpmI CTGGAG 1 cut(s) 1482
Bpu1102I GCTNAGC 1 cut(s) 1671
Bpu14I TTCGAA 2 cut(s) 157, 449
BsaBI GATNNNNATC 1 cut(s) 210
BsaHI GRCGYC 2 cut(s) 384, 1196
BsaI GGTCTC 1 cut(s) 1815
BsaJI CCNNGG 4 cut(s) 671, 1190, 1657, 1938
BsaWI WCCGGW 3 cut(s) 232, 394, 713
BsaXI ACNNNNNCTCC 2 cut(s) 1830, 1860
Bsc4I CCNNNNNNNGG 6 cut(s) 935, 1030, 1130, 1284, 1817, 1835
Bse118I RCCGGY 1 cut(s) 219
Bse1I ACTGG 8 cut(s) 366, 491, 509, 596, 1249, 1343, 1701, 1721
Bse21I CCTNAGG 1 cut(s) 1443
Bse3DI GCAATG 1 cut(s) 1875
Bse8I GATNNNNATC 1 cut(s) 210
BseAI TCCGGA 1 cut(s) 232
BseBI CCWGG 1 cut(s) 672
BseDI CCNNGG 4 cut(s) 671, 1190, 1657, 1938
BseGI GGATG 4 cut(s) 24, 1108, 1216, 1993
BseJI GATNNNNATC 1 cut(s) 210
BseLI CCNNNNNNNGG 6 cut(s) 935, 1030, 1130, 1284, 1817, 1835
BseMI GCAATG 1 cut(s) 1875
BseMII CTCAG 4 cut(s) 179, 993, 1662, 2040
BseNI ACTGG 8 cut(s) 366, 491, 509, 596, 1249, 1343, 1701, 1721
BseRI GAGGAG 2 cut(s) 175, 1872
BseSI GKGCMC 1 cut(s) 51
BseX3I CGGCCG 1 cut(s) 221
BseXI GCAGC 5 cut(s) 581, 829, 1759, 1774, 1900
BseYI CCCAGC 2 cut(s) 1123, 1647
BsgI GTGCAG 1 cut(s) 836
Bsh1236I CGCG 3 cut(s) 226, 228, 805
Bsh1285I CGRYCG 1 cut(s) 224
BshFI GGCC 8 cut(s) 223, 333, 365, 858, 1656, 1866, 2012, 2073
BsiEI CGRYCG 1 cut(s) 224
BsiHKAI GWGCWC 2 cut(s) 394, 542
BsiSI CCGG 6 cut(s) 220, 233, 265, 334, 395, 714
BslFI GGGAC 5 cut(s) 1210, 1313, 1370, 1392, 1452
BslI CCNNNNNNNGG 6 cut(s) 935, 1030, 1130, 1284, 1817, 1835
BsmAI GTCTC 2 cut(s) 731, 1815
BsmFI GGGAC 5 cut(s) 1210, 1313, 1370, 1392, 1452
BsmI GAATGC 1 cut(s) 421
BsnI GGCC 8 cut(s) 223, 333, 365, 858, 1656, 1866, 2012, 2073
Bso31I GGTCTC 1 cut(s) 1815
Bsp119I TTCGAA 2 cut(s) 157, 449
Bsp1286I GDGCHC 3 cut(s) 51, 394, 542
Bsp13I TCCGGA 1 cut(s) 232
Bsp143I GATC 8 cut(s) 205, 211, 524, 940, 948, 1072, 1474, 1933
Bsp1720I GCTNAGC 1 cut(s) 1671
Bsp19I CCATGG 1 cut(s) 1190
BspANI GGCC 8 cut(s) 223, 333, 365, 858, 1656, 1866, 2012, 2073
BspCNI CTCAG 4 cut(s) 178, 994, 1663, 2041
BspEI TCCGGA 1 cut(s) 232
BspFNI CGCG 3 cut(s) 226, 228, 805
BspHI TCATGA 1 cut(s) 1711
BspLI GGNNCC 4 cut(s) 507, 706, 1372, 1440
BspMAI CTGCAG 1 cut(s) 418
BspPI GGATC 4 cut(s) 219, 935, 943, 1928
BspT104I TTCGAA 2 cut(s) 157, 449
BspTNI GGTCTC 1 cut(s) 1815
BsrBI CCGCTC 1 cut(s) 310
BsrDI GCAATG 1 cut(s) 1875
BsrFI RCCGGY 1 cut(s) 219
BsrI ACTGG 8 cut(s) 366, 491, 509, 596, 1249, 1343, 1701, 1721
BssAI RCCGGY 1 cut(s) 219
BssECI CCNNGG 4 cut(s) 671, 1190, 1657, 1938
BssMI GATC 8 cut(s) 205, 211, 524, 940, 948, 1072, 1474, 1933
BssNI GRCGYC 2 cut(s) 384, 1196
BssT1I CCWWGG 3 cut(s) 1190, 1657, 1938
Bst2UI CCWGG 1 cut(s) 672
Bst4CI ACNGT 3 cut(s) 407, 637, 655
Bst6I CTCTTC 2 cut(s) 193, 1173
BstACI GRCGYC 2 cut(s) 384, 1196
BstAPI GCANNNNNTGC 1 cut(s) 1112
BstBI TTCGAA 2 cut(s) 157, 449
BstC8I GCNNGC 5 cut(s) 300, 913, 958, 1060, 1953
BstDEI CTNAG 5 cut(s) 165, 1002, 1443, 1671, 2049
BstDSI CCRYGG 1 cut(s) 1190
BstEII GGTNACC 1 cut(s) 397
BstF5I GGATG 4 cut(s) 24, 1108, 1216, 1993
BstFNI CGCG 3 cut(s) 226, 228, 805
BstHHI GCGC 2 cut(s) 228, 1873
BstKTI GATC 8 cut(s) 208, 214, 527, 943, 951, 1075, 1477, 1936
BstMAI GTCTC 2 cut(s) 731, 1815
BstMBI GATC 8 cut(s) 205, 211, 524, 940, 948, 1072, 1474, 1933
BstMCI CGRYCG 1 cut(s) 224
BstMWI GCNNNNNNNGC 9 cut(s) 251, 330, 339, 461, 1112, 1195, 1653, 1877, 2009
BstNI CCWGG 1 cut(s) 672
BstNSI RCATGY 3 cut(s) 1794, 1853, 1930
BstPI GGTNACC 1 cut(s) 397
BstSCI CCNGG 1 cut(s) 670
BstSFI CTRYAG 1 cut(s) 414
BstSLI GKGCMC 1 cut(s) 51
BstUI CGCG 3 cut(s) 226, 228, 805
BstV1I GCAGC 5 cut(s) 581, 829, 1759, 1774, 1900
BstX2I RGATCY 1 cut(s) 948
BstXI CCANNNNNNTGG 1 cut(s) 1434
BstYI RGATCY 1 cut(s) 948
BstZI CGGCCG 1 cut(s) 221
Bsu36I CCTNAGG 1 cut(s) 1443
BsuI GTATCC 2 cut(s) 1097, 1388
BsuRI GGCC 8 cut(s) 223, 333, 365, 858, 1656, 1866, 2012, 2073
BtgI CCRYGG 1 cut(s) 1190
BtrI CACGTC 1 cut(s) 2064
BtsCI GGATG 4 cut(s) 24, 1108, 1216, 1993
BtsI GCAGTG 3 cut(s) 13, 1729, 1746
BtsIMutI CAGTG 7 cut(s) 13, 373, 394, 498, 1154, 1729, 1746
Cac8I GCNNGC 5 cut(s) 300, 913, 958, 1060, 1953
CaiI CAGNNNCTG 1 cut(s) 2048
CciI TCATGA 1 cut(s) 1711
CfoI GCGC 2 cut(s) 228, 1873
Cfr10I RCCGGY 1 cut(s) 219
Cfr13I GGNCC 6 cut(s) 217, 230, 995, 1439, 1865, 2071
CpoI CGGWCCG 1 cut(s) 230
CseI GACGC 2 cut(s) 373, 1204
Csp6I GTAC 1 cut(s) 1847
CspCI CAANNNNNGTGG 2 cut(s) 1203, 1238
CspI CGGWCCG 1 cut(s) 230
CviQI GTAC 1 cut(s) 1847
DdeI CTNAG 5 cut(s) 165, 1002, 1443, 1671, 2049
DpnI GATC 8 cut(s) 207, 213, 526, 942, 950, 1074, 1476, 1935
DpnII GATC 8 cut(s) 205, 211, 524, 940, 948, 1072, 1474, 1933
DraI TTTAAA 2 cut(s) 892, 1096
DrdI GACNNNNNNGTC 1 cut(s) 653
DseDI GACNNNNNNGTC 1 cut(s) 653
EaeI YGGCCR 3 cut(s) 221, 331, 856
EagI CGGCCG 1 cut(s) 221
Eam1104I CTCTTC 2 cut(s) 193, 1173
EarI CTCTTC 2 cut(s) 193, 1173
EciI GGCGGA 3 cut(s) 269, 393, 1163
EclXI CGGCCG 1 cut(s) 221
Eco130I CCWWGG 3 cut(s) 1190, 1657, 1938
Eco31I GGTCTC 1 cut(s) 1815
Eco32I GATATC 1 cut(s) 471
Eco47I GGWCC 4 cut(s) 217, 230, 995, 1439
Eco52I CGGCCG 1 cut(s) 221
Eco57I CTGAAG 1 cut(s) 40
Eco81I CCTNAGG 1 cut(s) 1443
Eco91I GGTNACC 1 cut(s) 397
EcoO109I RGGNCCY 2 cut(s) 995, 1439
EcoO65I GGTNACC 1 cut(s) 397
EcoRI GAATTC 1 cut(s) 659
EcoRII CCWGG 1 cut(s) 670
EcoRV GATATC 1 cut(s) 471
EcoT14I CCWWGG 3 cut(s) 1190, 1657, 1938
EcoT22I ATGCAT 1 cut(s) 767
ErhI CCWWGG 3 cut(s) 1190, 1657, 1938
FaqI GGGAC 5 cut(s) 1210, 1313, 1370, 1392, 1452
FauI CCCGC 2 cut(s) 303, 317
FauNDI CATATG 1 cut(s) 1400
Fnu4HI GCNGC 7 cut(s) 224, 243, 570, 818, 1773, 1788, 1914
FokI GGATG 4 cut(s) 11, 1115, 1203, 2000
Fsp4HI GCNGC 7 cut(s) 224, 243, 570, 818, 1773, 1788, 1914
FspBI CTAG 5 cut(s) 549, 872, 932, 1076, 1508
GlaI GCGC 2 cut(s) 227, 1872
GluI GCNGC 7 cut(s) 224, 243, 570, 818, 1773, 1788, 1914
GsaI CCCAGC 2 cut(s) 1127, 1651
GsuI CTGGAG 1 cut(s) 1482
HaeIII GGCC 8 cut(s) 223, 333, 365, 858, 1656, 1866, 2012, 2073
HapII CCGG 6 cut(s) 220, 233, 265, 334, 395, 714
HgaI GACGC 2 cut(s) 373, 1204
HhaI GCGC 2 cut(s) 228, 1873
Hin1I GRCGYC 2 cut(s) 384, 1196
Hin6I GCGC 2 cut(s) 226, 1871
HinP1I GCGC 2 cut(s) 226, 1871
HincII GTYRAC 1 cut(s) 1273
HindII GTYRAC 1 cut(s) 1273
HpaII CCGG 6 cut(s) 220, 233, 265, 334, 395, 714
HphI GGTGA 6 cut(s) 259, 409, 465, 950, 1541, 1954
Hpy166II GTNNAC 2 cut(s) 1273, 1364
Hpy8I GTNNAC 2 cut(s) 1273, 1364
Hpy99I CGWCG 1 cut(s) 1604
HpyAV CCTTC 9 cut(s) 64, 91, 97, 767, 1008, 1571, 1840, 1939, 2023
HpyCH4III ACNGT 3 cut(s) 407, 637, 655
HpyCH4IV ACGT 3 cut(s) 45, 841, 2063
HpyF10VI GCNNNNNNNGC 9 cut(s) 251, 330, 339, 461, 1112, 1195, 1653, 1877, 2009
HpyF3I CTNAG 5 cut(s) 165, 1002, 1443, 1671, 2049
HpySE526I ACGT 3 cut(s) 45, 841, 2063
Hsp92I GRCGYC 2 cut(s) 384, 1196
HspAI GCGC 2 cut(s) 226, 1871
Kpn2I TCCGGA 1 cut(s) 232
Kzo9I GATC 8 cut(s) 205, 211, 524, 940, 948, 1072, 1474, 1933
LmnI GCTCC 6 cut(s) 23, 267, 397, 1376, 1948, 2000
Lsp1109I GCAGC 5 cut(s) 581, 829, 1759, 1774, 1900
LweI GCATC 6 cut(s) 736, 1093, 1490, 1641, 1756, 2032
MaeI CTAG 5 cut(s) 549, 872, 932, 1076, 1508
MaeII ACGT 3 cut(s) 45, 841, 2063
MaeIII GTNAC 5 cut(s) 397, 1019, 1345, 1529, 1993
MalI GATC 8 cut(s) 207, 213, 526, 942, 950, 1074, 1476, 1935
MbiI CCGCTC 1 cut(s) 310
MboI GATC 8 cut(s) 205, 211, 524, 940, 948, 1072, 1474, 1933
MboII GAAGA 7 cut(s) 69, 82, 180, 1160, 1333, 1718, 2018
MfeI CAATTG 2 cut(s) 303, 831
MflI RGATCY 1 cut(s) 948
MhlI GDGCHC 3 cut(s) 51, 394, 542
MlsI TGGCCA 1 cut(s) 858
MluI ACGCGT 1 cut(s) 803
MluNI TGGCCA 1 cut(s) 858
MlyI GAGTC 4 cut(s) 733, 1268, 1385, 1414
MmeI TCCRAC 5 cut(s) 330, 498, 682, 778, 1820
Mox20I TGGCCA 1 cut(s) 858
Mph1103I ATGCAT 1 cut(s) 767
MroI TCCGGA 1 cut(s) 232
MroXI GAANNNNTTC 1 cut(s) 709
MscI TGGCCA 1 cut(s) 858
MslI CAYNNNNRTG 2 cut(s) 1140, 1755
Msp20I TGGCCA 1 cut(s) 858
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 6 cut(s) 220, 233, 265, 334, 395, 714
MspR9I CCNGG 1 cut(s) 672
MunI CAATTG 2 cut(s) 303, 831
Mva1269I GAATGC 1 cut(s) 421
MvaI CCWGG 1 cut(s) 672
MvnI CGCG 3 cut(s) 226, 228, 805
MwoI GCNNNNNNNGC 9 cut(s) 251, 330, 339, 461, 1112, 1195, 1653, 1877, 2009
NcoI CCATGG 1 cut(s) 1190
NdeI CATATG 1 cut(s) 1400
NdeII GATC 8 cut(s) 205, 211, 524, 940, 948, 1072, 1474, 1933
NlaIV GGNNCC 4 cut(s) 507, 706, 1372, 1440
NmuCI GTSAC 3 cut(s) 397, 1529, 1993
NsiI ATGCAT 1 cut(s) 767
NspI RCATGY 3 cut(s) 1794, 1853, 1930
NspV TTCGAA 2 cut(s) 157, 449
PagI TCATGA 1 cut(s) 1711
PcsI WCGNNNNNNNCGW 1 cut(s) 654
PctI GAATGC 1 cut(s) 421
PdmI GAANNNNTTC 1 cut(s) 709
PfeI GAWTC 9 cut(s) 60, 98, 159, 169, 259, 1006, 1079, 1682, 2045
PkrI GCNGC 7 cut(s) 225, 244, 571, 819, 1774, 1789, 1915
PleI GAGTC 4 cut(s) 733, 1268, 1384, 1413
PpsI GAGTC 4 cut(s) 733, 1268, 1384, 1413
PpuMI RGGWCCY 2 cut(s) 995, 1439
PshBI ATTAAT 1 cut(s) 1308
Psp1406I AACGTT 1 cut(s) 841
Psp5II RGGWCCY 2 cut(s) 995, 1439
Psp6I CCWGG 1 cut(s) 670
PspEI GGTNACC 1 cut(s) 397
PspFI CCCAGC 2 cut(s) 1123, 1647
PspGI CCWGG 1 cut(s) 670
PspN4I GGNNCC 4 cut(s) 507, 706, 1372, 1440
PspPI GGNCC 6 cut(s) 217, 230, 995, 1439, 1865, 2071
PspPPI RGGWCCY 2 cut(s) 995, 1439
PstI CTGCAG 1 cut(s) 418
PstNI CAGNNNCTG 1 cut(s) 2048
PsuI RGATCY 1 cut(s) 948
RsaI GTAC 1 cut(s) 1848
RsaNI GTAC 1 cut(s) 1847
RseI CAYNNNNRTG 2 cut(s) 1140, 1755
Rsr2I CGGWCCG 1 cut(s) 230
RsrII CGGWCCG 1 cut(s) 230
SatI GCNGC 7 cut(s) 224, 243, 570, 818, 1773, 1788, 1914
Sau3AI GATC 8 cut(s) 205, 211, 524, 940, 948, 1072, 1474, 1933
Sau96I GGNCC 6 cut(s) 217, 230, 995, 1439, 1865, 2071
SchI GAGTC 4 cut(s) 733, 1268, 1385, 1414
ScrFI CCNGG 1 cut(s) 672
SduI GDGCHC 3 cut(s) 51, 394, 542
SfaNI GCATC 6 cut(s) 736, 1093, 1490, 1641, 1756, 2032
SfcI CTRYAG 1 cut(s) 414
SfuI TTCGAA 2 cut(s) 157, 449
SinI GGWCC 4 cut(s) 217, 230, 995, 1439
SmiMI CAYNNNNRTG 2 cut(s) 1140, 1755
SspMI CTAG 5 cut(s) 549, 872, 932, 1076, 1508
StyD4I CCNGG 1 cut(s) 670
StyI CCWWGG 3 cut(s) 1190, 1657, 1938
TaaI ACNGT 3 cut(s) 407, 637, 655
TaiI ACGT 3 cut(s) 48, 844, 2066
TaqI TCGA 8 cut(s) 37, 157, 449, 582, 657, 663, 1602, 2081
TatI WGTACW 1 cut(s) 1846
TauI GCSGC 2 cut(s) 226, 245
TfiI GAWTC 9 cut(s) 60, 98, 159, 169, 259, 1006, 1079, 1682, 2045
TscAI CASTG 7 cut(s) 20, 373, 394, 498, 1161, 1736, 1746
TseFI GTSAC 3 cut(s) 397, 1529, 1993
TseI GCWGC 5 cut(s) 569, 817, 1772, 1787, 1913
Tsp45I GTSAC 3 cut(s) 397, 1529, 1993
TspDTI ATGAA 3 cut(s) 17, 498, 1160
TspGWI ACGGA 3 cut(s) 372, 548, 663
TspRI CASTG 7 cut(s) 20, 373, 394, 498, 1161, 1736, 1746
VpaK11BI GGWCC 4 cut(s) 217, 230, 995, 1439
VspI ATTAAT 1 cut(s) 1308
XapI RAATTY 5 cut(s) 152, 591, 659, 886, 1208
XbaI TCTAGA 1 cut(s) 1075
XceI RCATGY 3 cut(s) 1794, 1853, 1930
XcmI CCANNNNNNNNNTGG 1 cut(s) 1693
XmnI GAANNNNTTC 1 cut(s) 709
XspI CTAG 5 cut(s) 549, 872, 932, 1076, 1508
Zsp2I ATGCAT 1 cut(s) 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.