Rw6G024420

Sterol 3-beta-glucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001484
Physical Location & Seq
Forward (+)
47475951 .. 47484266
8316 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G024420.1

Sequence Viewer

Length: 1737 bp
ATGCATTCCATCCCTAGTACGTTACTTGCGACCTTTGTTGGTGTTCAACTTCAAGTACTATCTTCCTTTTCGAAAACGAACGCATTGCCAGTGGAGATATCACCGACTCATGAAAGGCCAGAACCACATTCAGTTCCAGCAAAGTTGGAGAGATCAAAAACGGAGAGCACCAGACCTAATGTATTGTCCAAAGAGGCAGCAAAGATTTTTGACGACAAAACTTCAGTCCATCAGAAGTTAAAGCTGTTAAATAGAATAGCCACTGTGAAAGATGACGGAACTGTAGAATTCGATGTTCCAGGGGATGTTGAATCTGAATCTTTTGGTGTTGGATCCACTGCTGGTTGTACCGATGTTGCTGATGAGCCTCTTGATGCAACAGAGCTCCAATGCATACCACCAATGCAAATTGTAATGCTTATTGTTGGAACGCGTGGAGATGTGCAGCCGTTTATTGCAATTGGCAAGCGTTTGCAGGACTATGGCCATCGTGTAAGACTAGCTACTCATTCAAATTTTAAAGAGTTTGTCTTGACTGCTGGTTTGGAGTTCTACCCTTTAGGCGGTGATCCAAAAGTTCTTGCTGGCTATATGGTTAAAAATAAAGGCTTCTTGCCATCAGGGCCTTCGGAGATTCCTATTCAGCGTAACCAAATAAAGGAAATTATATATTCCTTACTTCCGGCTTGCAAAGAGCCTGATGTAGATTCTGGTATCCCCTTTAAAGCGGATGCAATTATTGCAAACCCCCCAGCATATGGGCATACTCATGTGGCGGAGGCACTGAAAATACCTCTTCATATATTTTTTACAATGCCATGGACGCCAACAAGTGAATTTCCACATCCCTTGTCCCGCGTTAAACAACAAACTGGATATAGACTATCATATCAAATTGTTGACTCCTTAATATGGCTTGGAATACGGGACATGATTAATGATGTTCGGAAGAAAAAATTGAGATTACGACCTGTCACATATTTAAGTGGTTCACAAGGCTCTGAGTCTGATGTCCCACATGGATACATATGGAGTCCTCATCTTGTTCCCAAACCAAAAGATTGGGGACCTAAGGTTGATGTGGTGGGATTTTGCTTCCTTGATCTTGCATCAAATTATGAACCTCCACAACCACTAGTGGAATGGCTTAAAGCTGGTGACAAGCCTATATACATTGGGTTTGGTAGCCTTCCTGTTCAAGAGCCAGAGAAAATGACCCAAATAATCGTCGATGCATTAGAAAAGACAGGACAGAGAGGCATCATTAACAAAGGCTGGGGTGGCCTTGGCAACTTGGCTGAACCAAAGGAATCCATATACTTATTAGACAACTGCCCTCATGACTGGCTGTTTCTACAATGCAAGGCAGTGGTGCATCATGGAGGTGCTGGAACAACGGCTGCTGGTCTAAAAGCTGCATGTCCGACAACAATTGTTCCTTTCTTTGGAGACCAACCCTTCTGGGGAGAGCGAGTACATGCCAGGGGAGTAGGCCCTGCACCCATTGCAGTTGATGAGTTCTCACTTCCCAAATTGGTTGATGCTATAAATTACATGCTTGATCCCAAGGTGAAGGAGCGTGCTATTGAACTTGCCAAGGATATGGAGAATGAGGATGGTGTGACAGGAGCAGTCATGGCCTTCTTTAAGCATCTTCCTCGCAAAAAGCCCGAGTCTGAGCCAGAAACAGAGTCATCGAGCCTTTGCTCTATAAGTAGATGTTTTGGTTGTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_transf_28 UDPGT

Protein Analysis

578

Amino Acids

63.51

Weight (kDa)

6.21

Isoelectric Point (pI)

39.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_28 PF03033 137 - 280 4.5e-35 Glycosyltransferase family 28 N-terminal domain
UDPGT PF00201 386 - 532 4.2e-06 UDP-glucoronosyl and UDP-glucosyl transferase
EryCIII-like_C PF06722 437 - 537 1.7e-11 Erythromycin biosynthesis protein CIII-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 758
AccII CGCG 2 cut(s) 433, 858
AciI CCGC 4 cut(s) 564, 728, 776, 856
AclWI GGATC 4 cut(s) 327, 340, 563, 1556
AcoI YGGCCR 1 cut(s) 484
AcsI RAATTY 3 cut(s) 287, 514, 836
AcuI CTGAAG 1 cut(s) 207
AcyI GRCGYC 1 cut(s) 824
AfaI GTAC 4 cut(s) 19, 57, 349, 1476
AfiI CCNNNNNNNGG 6 cut(s) 563, 658, 758, 912, 1445, 1463
AflIII ACRYGT 1 cut(s) 431
AgsI TTSAA 6 cut(s) 47, 53, 311, 513, 1199, 1589
AhlI ACTAGT 1 cut(s) 1135
AjnI CCWGG 2 cut(s) 298, 1481
AluBI AGCT 5 cut(s) 244, 385, 503, 1154, 1415
AluI AGCT 5 cut(s) 244, 385, 503, 1154, 1415
Alw21I GWGCWC 2 cut(s) 170, 387
Alw26I GTCTC 1 cut(s) 1443
AlwI GGATC 4 cut(s) 327, 340, 563, 1556
Ama87I CYCGRG 1 cut(s) 1670
AoxI GGCC 6 cut(s) 116, 484, 623, 1282, 1492, 1638
ApeKI GCWGC 4 cut(s) 197, 445, 1400, 1415
ApoI RAATTY 3 cut(s) 287, 514, 836
ArsI GACNNNNNNTTYG 4 cut(s) 210, 242, 526, 558
AseI ATTAAT 1 cut(s) 936
AspS9I GGNCC 3 cut(s) 623, 1067, 1493
AsuHPI GGTGA 4 cut(s) 93, 578, 1169, 1582
AsuII TTCGAA 1 cut(s) 71
AvaI CYCGRG 1 cut(s) 1670
AvaII GGWCC 1 cut(s) 1067
AxyI CCTNAGG 1 cut(s) 1071
BalI TGGCCA 1 cut(s) 486
BamHI GGATCC 1 cut(s) 332
BanII GRGCYC 1 cut(s) 387
Bbv12I GWGCWC 2 cut(s) 170, 387
BbvI GCAGC 4 cut(s) 209, 457, 1387, 1402
BccI CCATC 5 cut(s) 17, 237, 495, 625, 1610
BceAI ACGGC 2 cut(s) 433, 1413
BcgI CGANNNNNNTGC 4 cut(s) 67, 101, 1640, 1674
BciT130I CCWGG 2 cut(s) 300, 1483
BciVI GTATCC 2 cut(s) 725, 1016
BcoDI GTCTC 1 cut(s) 1443
BcuI ACTAGT 1 cut(s) 1135
BfaI CTAG 3 cut(s) 15, 500, 1136
BfmI CTRYAG 1 cut(s) 282
BfuI GTATCC 2 cut(s) 725, 1016
BglI GCCNNNNNGGC 1 cut(s) 622
BisI GCNGC 4 cut(s) 198, 446, 1401, 1416
BlsI GCNGC 4 cut(s) 199, 447, 1402, 1417
BmcAI AGTACT 1 cut(s) 57
Bme1390I CCNGG 2 cut(s) 300, 1483
Bme18I GGWCC 1 cut(s) 1067
BmeT110I CYCGRG 1 cut(s) 1670
BmgT120I GGNCC 3 cut(s) 623, 1067, 1493
BmiI GGNNCC 2 cut(s) 334, 1068
BmrFI CCNGG 2 cut(s) 300, 1483
BmsI GCATC 8 cut(s) 364, 721, 1118, 1222, 1269, 1384, 1531, 1660
Bpu14I TTCGAA 1 cut(s) 71
BsaHI GRCGYC 1 cut(s) 824
BsaI GGTCTC 1 cut(s) 1443
BsaJI CCNNGG 6 cut(s) 299, 818, 1285, 1482, 1566, 1596
BsaXI ACNNNNNCTCC 2 cut(s) 1458, 1488
Bsc4I CCNNNNNNNGG 6 cut(s) 563, 658, 758, 912, 1445, 1463
Bse1I ACTGG 3 cut(s) 89, 877, 1349
Bse21I CCTNAGG 1 cut(s) 1071
Bse3DI GCAATG 2 cut(s) 83, 1503
BseBI CCWGG 2 cut(s) 300, 1483
BseDI CCNNGG 6 cut(s) 299, 818, 1285, 1482, 1566, 1596
BseGI GGATG 5 cut(s) 9, 310, 736, 844, 1621
BseLI CCNNNNNNNGG 6 cut(s) 563, 658, 758, 912, 1445, 1463
BseMI GCAATG 2 cut(s) 83, 1503
BseMII CTCAG 2 cut(s) 993, 1668
BseNI ACTGG 3 cut(s) 89, 877, 1349
BseXI GCAGC 4 cut(s) 209, 457, 1387, 1402
BseYI CCCAGC 2 cut(s) 751, 1275
BsgI GTGCAG 2 cut(s) 464, 1482
Bsh1236I CGCG 2 cut(s) 433, 858
BshFI GGCC 6 cut(s) 118, 486, 625, 1284, 1494, 1640
BsiHKAI GWGCWC 2 cut(s) 170, 387
BsiHKCI CYCGRG 1 cut(s) 1670
BsiSI CCGG 1 cut(s) 683
BslFI GGGAC 4 cut(s) 838, 941, 998, 1080
BslI CCNNNNNNNGG 6 cut(s) 563, 658, 758, 912, 1445, 1463
BsmAI GTCTC 1 cut(s) 1443
BsmFI GGGAC 4 cut(s) 838, 941, 998, 1080
BsmI GAATGC 1 cut(s) 4
BsnI GGCC 6 cut(s) 118, 486, 625, 1284, 1494, 1640
Bso31I GGTCTC 1 cut(s) 1443
BsoBI CYCGRG 1 cut(s) 1670
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 2 cut(s) 170, 387
Bsp143I GATC 5 cut(s) 152, 332, 568, 1102, 1561
Bsp19I CCATGG 1 cut(s) 818
BspACI CCGC 4 cut(s) 564, 728, 776, 856
BspANI GGCC 6 cut(s) 118, 486, 625, 1284, 1494, 1640
BspCNI CTCAG 2 cut(s) 994, 1669
BspFNI CGCG 2 cut(s) 433, 858
BspHI TCATGA 2 cut(s) 109, 1339
BspLI GGNNCC 2 cut(s) 334, 1068
BspPI GGATC 4 cut(s) 327, 340, 563, 1556
BspT104I TTCGAA 1 cut(s) 71
BspTNI GGTCTC 1 cut(s) 1443
BsrDI GCAATG 2 cut(s) 83, 1503
BsrI ACTGG 3 cut(s) 89, 877, 1349
BssECI CCNNGG 6 cut(s) 299, 818, 1285, 1482, 1566, 1596
BssMI GATC 5 cut(s) 152, 332, 568, 1102, 1561
BssNI GRCGYC 1 cut(s) 824
BssT1I CCWWGG 4 cut(s) 818, 1285, 1566, 1596
Bst2UI CCWGG 2 cut(s) 300, 1483
Bst4CI ACNGT 2 cut(s) 265, 283
Bst6I CTCTTC 1 cut(s) 801
BstACI GRCGYC 1 cut(s) 824
BstAPI GCANNNNNTGC 2 cut(s) 740, 1505
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 4 cut(s) 467, 586, 688, 1581
BstDEI CTNAG 3 cut(s) 1002, 1071, 1677
BstDSI CCRYGG 1 cut(s) 818
BstF5I GGATG 5 cut(s) 9, 310, 736, 844, 1621
BstFNI CGCG 2 cut(s) 433, 858
BstKTI GATC 5 cut(s) 155, 335, 571, 1105, 1564
BstMAI GTCTC 1 cut(s) 1443
BstMBI GATC 5 cut(s) 152, 332, 568, 1102, 1561
BstMWI GCNNNNNNNGC 6 cut(s) 622, 740, 823, 1281, 1505, 1637
BstNI CCWGG 2 cut(s) 300, 1483
BstNSI RCATGY 3 cut(s) 1422, 1481, 1558
BstSCI CCNGG 2 cut(s) 298, 1481
BstSFI CTRYAG 1 cut(s) 282
BstUI CGCG 2 cut(s) 433, 858
BstV1I GCAGC 4 cut(s) 209, 457, 1387, 1402
BstX2I RGATCY 1 cut(s) 332
BstXI CCANNNNNNTGG 2 cut(s) 1062, 1603
BstYI RGATCY 1 cut(s) 332
Bsu36I CCTNAGG 1 cut(s) 1071
BsuI GTATCC 2 cut(s) 725, 1016
BsuRI GGCC 6 cut(s) 118, 486, 625, 1284, 1494, 1640
BtgI CCRYGG 1 cut(s) 818
BtsCI GGATG 5 cut(s) 9, 310, 736, 844, 1621
BtsI GCAGTG 2 cut(s) 336, 1374
BtsIMutI CAGTG 5 cut(s) 96, 261, 336, 782, 1374
Cac8I GCNNGC 4 cut(s) 467, 586, 688, 1581
CciI TCATGA 2 cut(s) 109, 1339
Cfr13I GGNCC 3 cut(s) 623, 1067, 1493
CseI GACGC 1 cut(s) 832
Csp6I GTAC 4 cut(s) 18, 56, 348, 1475
CspCI CAANNNNNGTGG 2 cut(s) 831, 866
CviQI GTAC 4 cut(s) 18, 56, 348, 1475
DdeI CTNAG 3 cut(s) 1002, 1071, 1677
DpnI GATC 5 cut(s) 154, 334, 570, 1104, 1563
DpnII GATC 5 cut(s) 152, 332, 568, 1102, 1561
DraI TTTAAA 2 cut(s) 520, 724
EaeI YGGCCR 1 cut(s) 484
Eam1104I CTCTTC 1 cut(s) 801
EarI CTCTTC 1 cut(s) 801
EciI GGCGGA 1 cut(s) 791
Ecl136II GAGCTC 1 cut(s) 385
Eco130I CCWWGG 4 cut(s) 818, 1285, 1566, 1596
Eco24I GRGCYC 1 cut(s) 387
Eco31I GGTCTC 1 cut(s) 1443
Eco32I GATATC 1 cut(s) 99
Eco47I GGWCC 1 cut(s) 1067
Eco53kI GAGCTC 1 cut(s) 385
Eco57I CTGAAG 1 cut(s) 207
Eco81I CCTNAGG 1 cut(s) 1071
Eco88I CYCGRG 1 cut(s) 1670
EcoICRI GAGCTC 1 cut(s) 385
EcoO109I RGGNCCY 3 cut(s) 623, 1067, 1493
EcoRI GAATTC 1 cut(s) 287
EcoRII CCWGG 2 cut(s) 298, 1481
EcoRV GATATC 1 cut(s) 99
EcoT14I CCWWGG 4 cut(s) 818, 1285, 1566, 1596
EcoT22I ATGCAT 3 cut(s) 6, 395, 1237
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 4 cut(s) 818, 1285, 1566, 1596
FaqI GGGAC 4 cut(s) 838, 941, 998, 1080
FauI CCCGC 1 cut(s) 863
FauNDI CATATG 2 cut(s) 757, 1028
Fnu4HI GCNGC 4 cut(s) 198, 446, 1401, 1416
FokI GGATG 4 cut(s) 317, 743, 831, 1628
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 4 cut(s) 198, 446, 1401, 1416
FspBI CTAG 3 cut(s) 15, 500, 1136
GluI GCNGC 4 cut(s) 198, 446, 1401, 1416
GsaI CCCAGC 2 cut(s) 755, 1279
HaeIII GGCC 6 cut(s) 118, 486, 625, 1284, 1494, 1640
HapII CCGG 1 cut(s) 683
HgaI GACGC 1 cut(s) 832
Hin1I GRCGYC 1 cut(s) 824
HincII GTYRAC 1 cut(s) 901
HindII GTYRAC 1 cut(s) 901
HpaII CCGG 1 cut(s) 683
HphI GGTGA 4 cut(s) 93, 578, 1169, 1582
Hpy166II GTNNAC 2 cut(s) 901, 992
Hpy188I TCNGA 8 cut(s) 234, 316, 631, 948, 1003, 1009, 1425, 1678
Hpy188III TCNNGA 6 cut(s) 110, 371, 532, 1199, 1340, 1734
Hpy8I GTNNAC 2 cut(s) 901, 992
Hpy99I CGWCG 1 cut(s) 1232
HpyAV CCTTC 5 cut(s) 636, 1199, 1468, 1567, 1651
HpyCH4III ACNGT 2 cut(s) 265, 283
HpyCH4IV ACGT 1 cut(s) 20
HpyF10VI GCNNNNNNNGC 6 cut(s) 622, 740, 823, 1281, 1505, 1637
HpyF3I CTNAG 3 cut(s) 1002, 1071, 1677
HpySE526I ACGT 1 cut(s) 20
Hsp92I GRCGYC 1 cut(s) 824
Kzo9I GATC 5 cut(s) 152, 332, 568, 1102, 1561
LmnI GCTCC 3 cut(s) 390, 1576, 1628
Lsp1109I GCAGC 4 cut(s) 209, 457, 1387, 1402
LweI GCATC 8 cut(s) 364, 721, 1118, 1222, 1269, 1384, 1531, 1660
MaeI CTAG 3 cut(s) 15, 500, 1136
MaeII ACGT 1 cut(s) 20
MaeIII GTNAC 5 cut(s) 21, 647, 973, 1157, 1621
MalI GATC 5 cut(s) 154, 334, 570, 1104, 1563
MboI GATC 5 cut(s) 152, 332, 568, 1102, 1561
MboII GAAGA 4 cut(s) 54, 788, 961, 1646
MfeI CAATTG 2 cut(s) 459, 1431
MflI RGATCY 1 cut(s) 332
MhlI GDGCHC 2 cut(s) 170, 387
MlsI TGGCCA 1 cut(s) 486
MluI ACGCGT 1 cut(s) 431
MluNI TGGCCA 1 cut(s) 486
MlyI GAGTC 6 cut(s) 100, 896, 1013, 1042, 1682, 1700
MmeI TCCRAC 4 cut(s) 126, 310, 406, 1448
Mox20I TGGCCA 1 cut(s) 486
Mph1103I ATGCAT 3 cut(s) 6, 395, 1237
MscI TGGCCA 1 cut(s) 486
MslI CAYNNNNRTG 2 cut(s) 768, 1383
Msp20I TGGCCA 1 cut(s) 486
MspI CCGG 1 cut(s) 683
MspR9I CCNGG 2 cut(s) 300, 1483
MunI CAATTG 2 cut(s) 459, 1431
Mva1269I GAATGC 1 cut(s) 4
MvaI CCWGG 2 cut(s) 300, 1483
MvnI CGCG 2 cut(s) 433, 858
MwoI GCNNNNNNNGC 6 cut(s) 622, 740, 823, 1281, 1505, 1637
NcoI CCATGG 1 cut(s) 818
NdeI CATATG 2 cut(s) 757, 1028
NdeII GATC 5 cut(s) 152, 332, 568, 1102, 1561
NlaIV GGNNCC 2 cut(s) 334, 1068
NmuCI GTSAC 3 cut(s) 973, 1157, 1621
NsiI ATGCAT 3 cut(s) 6, 395, 1237
NspI RCATGY 3 cut(s) 1422, 1481, 1558
NspV TTCGAA 1 cut(s) 71
PagI TCATGA 2 cut(s) 109, 1339
PcsI WCGNNNNNNNCGW 1 cut(s) 26
PctI GAATGC 1 cut(s) 4
PfeI GAWTC 5 cut(s) 311, 317, 634, 707, 1310
PflMI CCANNNNNTGG 1 cut(s) 758
PkrI GCNGC 4 cut(s) 199, 447, 1402, 1417
PleI GAGTC 6 cut(s) 100, 896, 1012, 1041, 1681, 1699
PpsI GAGTC 6 cut(s) 100, 896, 1012, 1041, 1681, 1699
PpuMI RGGWCCY 1 cut(s) 1067
PshBI ATTAAT 1 cut(s) 936
Psp124BI GAGCTC 1 cut(s) 387
Psp5II RGGWCCY 1 cut(s) 1067
Psp6I CCWGG 2 cut(s) 298, 1481
PspFI CCCAGC 2 cut(s) 751, 1275
PspGI CCWGG 2 cut(s) 298, 1481
PspN4I GGNNCC 2 cut(s) 334, 1068
PspPI GGNCC 3 cut(s) 623, 1067, 1493
PspPPI RGGWCCY 1 cut(s) 1067
PsuI RGATCY 1 cut(s) 332
RsaI GTAC 4 cut(s) 19, 57, 349, 1476
RsaNI GTAC 4 cut(s) 18, 56, 348, 1475
RseI CAYNNNNRTG 2 cut(s) 768, 1383
SacI GAGCTC 1 cut(s) 387
SatI GCNGC 4 cut(s) 198, 446, 1401, 1416
Sau3AI GATC 5 cut(s) 152, 332, 568, 1102, 1561
Sau96I GGNCC 3 cut(s) 623, 1067, 1493
ScaI AGTACT 1 cut(s) 57
SchI GAGTC 6 cut(s) 100, 896, 1013, 1042, 1682, 1700
ScrFI CCNGG 2 cut(s) 300, 1483
SduI GDGCHC 2 cut(s) 170, 387
SfaNI GCATC 8 cut(s) 364, 721, 1118, 1222, 1269, 1384, 1531, 1660
SfcI CTRYAG 1 cut(s) 282
SfuI TTCGAA 1 cut(s) 71
SinI GGWCC 1 cut(s) 1067
SmiMI CAYNNNNRTG 2 cut(s) 768, 1383
SpeI ACTAGT 1 cut(s) 1135
SsiI CCGC 4 cut(s) 564, 728, 776, 856
SspMI CTAG 3 cut(s) 15, 500, 1136
SstI GAGCTC 1 cut(s) 387
StyD4I CCNGG 2 cut(s) 298, 1481
StyI CCWWGG 4 cut(s) 818, 1285, 1566, 1596
TaaI ACNGT 2 cut(s) 265, 283
TaiI ACGT 1 cut(s) 23
TaqI TCGA 4 cut(s) 71, 291, 1230, 1697
TatI WGTACW 2 cut(s) 55, 1474
TfiI GAWTC 5 cut(s) 311, 317, 634, 707, 1310
TscAI CASTG 5 cut(s) 96, 268, 343, 789, 1374
TseFI GTSAC 3 cut(s) 973, 1157, 1621
TseI GCWGC 4 cut(s) 197, 445, 1400, 1415
Tsp45I GTSAC 3 cut(s) 973, 1157, 1621
TspDTI ATGAA 3 cut(s) 126, 788, 1134
TspGWI ACGGA 2 cut(s) 176, 291
TspRI CASTG 5 cut(s) 96, 268, 343, 789, 1374
Van91I CCANNNNNTGG 1 cut(s) 758
VpaK11BI GGWCC 1 cut(s) 1067
VspI ATTAAT 1 cut(s) 936
XapI RAATTY 3 cut(s) 287, 514, 836
XceI RCATGY 3 cut(s) 1422, 1481, 1558
XcmI CCANNNNNNNNNTGG 1 cut(s) 1140
XspI CTAG 3 cut(s) 15, 500, 1136
ZrmI AGTACT 1 cut(s) 57
Zsp2I ATGCAT 3 cut(s) 6, 395, 1237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.