RLG00000012659

Sterol 3-beta-glucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001484
Physical Location & Seq
Reverse (-)
21006613 .. 21014173
7561 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012659

Sequence Viewer

Length: 1848 bp
ATGGCGGAGGATCGCCGGAGTAGTTCCGAGAGTTCTGTAGAAGTTCCGGGGAGAATTGAGCAGGAAATTGACGGCGGAAGTGGCGGGAAAAGAATAGAGTTGGAGAACGGAGAGGCTAGTGGCGGAGGCGGAGGCGGAGGCGTTACTGCTCCGGTGAATAACGGCGGCGGTAGTAGCTCTACAGGGGATTCAGGAAATTGTGGCTATAGCTTTTCGAAAGCGAACGCATTGCCAGTGGAGATATCACCGACTCATGAAAGGCCAGAACCACATTCAGTTCCAGCAAAGTTGGAGAGATCAAAAACGGAGAGCACCAGACCTAATGTATTGCCCAAAGAGGCAGCAAAGATTTTTGACGACAAAACTTCAGTCCATCAGAAGTTAAAGCTGTTAAATAGAATTGCTACTGTGAAAGATGACGGAACTGTAGAATTCGATGTTCCAGGGGATGTTGAATCTCAATCTTTTGGTGTTGGATCCACTGCTGGTTGTACCGATGTTGCTGATGAGCCTCTTGATGCAACAGACCTCCAATGCATACCACCCATGCAAATAGTAATGCTTATAGTTGGAACGCGTGGAGATGTGCAGCCGTTTATTGCAATTGGCAAACGTTTGCAGGACTATGGCCATCGTGTAAGACTAGCTACTCATTCAAATTTTAAAGAGTTTGTCTTGACTGCTGGTTTGGAGTTCTACCCTTTAGGCGGTGATCCAAAAGTTCTTGCTGGCTATATGGTTAAAAATAAAGGCTTCTTGCCGTCAGGTCCTTCGGAGATTCCTATTCAGCGTAACCAAATAAAGGAAATTATATACTCCTTACTTCCAGCTTGCAAAGAGCCTGATGTAGATTCTGGTATTCCCTTTAAAGCGGATGCAATTATTGCAAACCCCCCAGCATATGTTTGTAGGTTGTCTGAGCTATTAACTTCCAAGATACTTATGCATATTATTCTATCGTATCAAATTGTTGACTCCTTAATATGGCTTGGAATACGGGACATGATTAATGATGTTCGGAAGAAAAAATTGAAATTACGACCCGTCACATATTTAAGTGGTTCACAAGGCTCCGAGTCTGATGTCCCACATGGATACATATGGAGTCCTCATCTTGTTCCCAAACCAAAAGATTGGGGACCGAAGGTTGATGTGGTGGGATTTTGCTTCCTTGATCTTGCATCAAATTATGAACCTCCACAACCACTAGTGGAATGGCTTAAAGCTGGTGACAAGCCTATATACATTGGGTTTGGTAGCCTTCCTGTTCAAGAGCCAGAGAAAATGACCCAAATAATCGTCGATGCATTAGAAAAGACAGGACAGAGAGGCATCATTAACAAAGGCTGGGGTGGCCTTGGGAACTTGGCTGAACCGAAGGAATCCATATACTTATTGGACAACTGCCCTCATGACTGGCTGTTTCTACAATGCAAGGCAGTGGTGCATCATGGAGGTGCTGGAACAACGGCTGCTGGTCTAAAAGCTGCATGTCCAACAACAATTGTTCCTTTCTTTGGAGACCAACCCTTCTGGGGAGAGCGAGTACATGCCAGGGGAGTAGGCCCTGCACCCATTGCAGTTGATGAGTTCTCACTTCCCAAATTGATTGATGCTATAAATTACATGCTTGATCCCAAGGTGAAGGAGCGTGCTATTGAACTTGCCAAGGATATGGAGAATGAGGATGGTGTGACAGGAGCAGTCATGGCCTTCTTTAAGCATCTTCCTCGCAAAAAGCCCGAGTCTGAGCCAGAAACAGAGTCATCGAGCCTTTGCTCTATAAGAAACCTTGTCTTCCTATCGAAGATGATGTCAATTCTTGGCTCTCATTCGGCTCAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_transf_28 UDPGT

Protein Analysis

616

Amino Acids

66.52

Weight (kDa)

5.71

Isoelectric Point (pI)

46.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_28 PF03033 185 - 302 1.4e-24 Glycosyltransferase family 28 N-terminal domain
UDPGT PF00201 403 - 556 3.7e-06 UDP-glucoronosyl and UDP-glucosyl transferase
EryCIII-like_C PF06722 462 - 561 2.3e-11 Erythromycin biosynthesis protein CIII-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 577
AclI AACGTT 1 cut(s) 613
AclWI GGATC 5 cut(s) 18, 471, 484, 707, 1628
AcoI YGGCCR 1 cut(s) 628
AcsI RAATTY 2 cut(s) 431, 658
AcuI CTGAAG 1 cut(s) 351
AfaI GTAC 2 cut(s) 493, 1548
AfiI CCNNNNNNNGG 5 cut(s) 707, 802, 984, 1517, 1535
AflIII ACRYGT 1 cut(s) 575
AgsI TTSAA 5 cut(s) 455, 657, 1033, 1271, 1661
AhlI ACTAGT 1 cut(s) 1207
AjnI CCWGG 2 cut(s) 442, 1553
AluBI AGCT 8 cut(s) 177, 210, 388, 647, 830, 922, 1226, 1487
AluI AGCT 8 cut(s) 177, 210, 388, 647, 830, 922, 1226, 1487
Alw21I GWGCWC 1 cut(s) 314
Alw26I GTCTC 1 cut(s) 1515
AlwI GGATC 5 cut(s) 18, 471, 484, 707, 1628
Ama87I CYCGRG 1 cut(s) 1742
AoxI GGCC 5 cut(s) 260, 628, 1354, 1564, 1710
ApeKI GCWGC 4 cut(s) 341, 589, 1472, 1487
ApoI RAATTY 2 cut(s) 431, 658
ArsI GACNNNNNNTTYG 6 cut(s) 354, 386, 670, 702, 1799, 1831
AseI ATTAAT 1 cut(s) 1008
AspS9I GGNCC 3 cut(s) 767, 1139, 1565
AsuC2I CCSGG 1 cut(s) 48
AsuHPI GGTGA 5 cut(s) 166, 237, 722, 1241, 1654
AsuII TTCGAA 1 cut(s) 215
AvaI CYCGRG 1 cut(s) 1742
AvaII GGWCC 2 cut(s) 767, 1139
BalI TGGCCA 1 cut(s) 630
BamHI GGATCC 1 cut(s) 476
BbsI GAAGAC 1 cut(s) 1789
Bbv12I GWGCWC 1 cut(s) 314
BbvI GCAGC 4 cut(s) 353, 601, 1459, 1474
BccI CCATC 3 cut(s) 381, 639, 1682
BceAI ACGGC 5 cut(s) 88, 178, 577, 745, 1485
BcgI CGANNNNNNTGC 4 cut(s) 211, 245, 1712, 1746
BciT130I CCWGG 2 cut(s) 444, 1555
BciVI GTATCC 1 cut(s) 1088
BcnI CCSGG 1 cut(s) 48
BcoDI GTCTC 1 cut(s) 1515
BcuI ACTAGT 1 cut(s) 1207
BfaI CTAG 3 cut(s) 117, 644, 1208
BfmI CTRYAG 4 cut(s) 36, 180, 205, 426
BfuI GTATCC 1 cut(s) 1088
BisI GCNGC 5 cut(s) 166, 342, 590, 1473, 1488
BlsI GCNGC 5 cut(s) 167, 343, 591, 1474, 1489
Bme1390I CCNGG 3 cut(s) 48, 444, 1555
Bme18I GGWCC 2 cut(s) 767, 1139
BmeT110I CYCGRG 1 cut(s) 1742
BmgT120I GGNCC 3 cut(s) 767, 1139, 1565
BmiI GGNNCC 3 cut(s) 478, 1072, 1140
BmrFI CCNGG 3 cut(s) 48, 444, 1555
BmsI GCATC 8 cut(s) 508, 865, 1190, 1294, 1341, 1456, 1603, 1732
BpiI GAAGAC 1 cut(s) 1789
Bpu14I TTCGAA 1 cut(s) 215
BpuEI CTTGAG 1 cut(s) 1824
BpuMI CCSGG 1 cut(s) 48
BsaI GGTCTC 1 cut(s) 1515
BsaJI CCNNGG 6 cut(s) 47, 443, 1357, 1554, 1638, 1668
BsaWI WCCGGW 1 cut(s) 151
BsaXI ACNNNNNCTCC 2 cut(s) 1530, 1560
Bsc4I CCNNNNNNNGG 5 cut(s) 707, 802, 984, 1517, 1535
Bse1I ACTGG 2 cut(s) 233, 1421
Bse3DI GCAATG 2 cut(s) 227, 1575
BseBI CCWGG 2 cut(s) 444, 1555
BseDI CCNNGG 6 cut(s) 47, 443, 1357, 1554, 1638, 1668
BseGI GGATG 3 cut(s) 454, 880, 1693
BseLI CCNNNNNNNGG 5 cut(s) 707, 802, 984, 1517, 1535
BseMI GCAATG 2 cut(s) 227, 1575
BseMII CTCAG 2 cut(s) 909, 1740
BseNI ACTGG 2 cut(s) 233, 1421
BseXI GCAGC 4 cut(s) 353, 601, 1459, 1474
BseYI CCCAGC 2 cut(s) 895, 1347
BsgI GTGCAG 2 cut(s) 608, 1554
Bsh1236I CGCG 1 cut(s) 577
BshFI GGCC 5 cut(s) 262, 630, 1356, 1566, 1712
BsiHKAI GWGCWC 1 cut(s) 314
BsiHKCI CYCGRG 1 cut(s) 1742
BsiSI CCGG 3 cut(s) 16, 47, 152
BslFI GGGAC 3 cut(s) 1013, 1070, 1152
BslI CCNNNNNNNGG 5 cut(s) 707, 802, 984, 1517, 1535
BsmAI GTCTC 1 cut(s) 1515
BsmFI GGGAC 3 cut(s) 1013, 1070, 1152
BsnI GGCC 5 cut(s) 262, 630, 1356, 1566, 1712
Bso31I GGTCTC 1 cut(s) 1515
BsoBI CYCGRG 1 cut(s) 1742
Bsp119I TTCGAA 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 314
Bsp143I GATC 6 cut(s) 10, 296, 476, 712, 1174, 1633
BspANI GGCC 5 cut(s) 262, 630, 1356, 1566, 1712
BspCNI CTCAG 2 cut(s) 910, 1741
BspFNI CGCG 1 cut(s) 577
BspHI TCATGA 2 cut(s) 253, 1411
BspLI GGNNCC 3 cut(s) 478, 1072, 1140
BspPI GGATC 5 cut(s) 18, 471, 484, 707, 1628
BspT104I TTCGAA 1 cut(s) 215
BspTNI GGTCTC 1 cut(s) 1515
BsrDI GCAATG 2 cut(s) 227, 1575
BsrI ACTGG 2 cut(s) 233, 1421
BssECI CCNNGG 6 cut(s) 47, 443, 1357, 1554, 1638, 1668
BssMI GATC 6 cut(s) 10, 296, 476, 712, 1174, 1633
BssT1I CCWWGG 3 cut(s) 1357, 1638, 1668
Bst2UI CCWGG 2 cut(s) 444, 1555
Bst4CI ACNGT 2 cut(s) 409, 427
BstAPI GCANNNNNTGC 2 cut(s) 884, 1577
BstBI TTCGAA 1 cut(s) 215
BstC8I GCNNGC 3 cut(s) 730, 832, 1653
BstDEI CTNAG 2 cut(s) 918, 1749
BstF5I GGATG 3 cut(s) 454, 880, 1693
BstFNI CGCG 1 cut(s) 577
BstKTI GATC 6 cut(s) 13, 299, 479, 715, 1177, 1636
BstMAI GTCTC 1 cut(s) 1515
BstMBI GATC 6 cut(s) 10, 296, 476, 712, 1174, 1633
BstMWI GCNNNNNNNGC 6 cut(s) 81, 174, 884, 1353, 1577, 1709
BstNI CCWGG 2 cut(s) 444, 1555
BstNSI RCATGY 3 cut(s) 1494, 1553, 1630
BstSCI CCNGG 3 cut(s) 46, 442, 1553
BstSFI CTRYAG 4 cut(s) 36, 180, 205, 426
BstUI CGCG 1 cut(s) 577
BstV1I GCAGC 4 cut(s) 353, 601, 1459, 1474
BstV2I GAAGAC 1 cut(s) 1789
BstX2I RGATCY 1 cut(s) 476
BstXI CCANNNNNNTGG 2 cut(s) 1134, 1675
BstYI RGATCY 1 cut(s) 476
BsuI GTATCC 1 cut(s) 1088
BsuRI GGCC 5 cut(s) 262, 630, 1356, 1566, 1712
BtsCI GGATG 3 cut(s) 454, 880, 1693
BtsI GCAGTG 2 cut(s) 480, 1446
BtsIMutI CAGTG 3 cut(s) 240, 480, 1446
Cac8I GCNNGC 3 cut(s) 730, 832, 1653
CciI TCATGA 2 cut(s) 253, 1411
Cfr13I GGNCC 3 cut(s) 767, 1139, 1565
Csp6I GTAC 2 cut(s) 492, 1547
CviQI GTAC 2 cut(s) 492, 1547
DdeI CTNAG 2 cut(s) 918, 1749
DpnI GATC 6 cut(s) 12, 298, 478, 714, 1176, 1635
DpnII GATC 6 cut(s) 10, 296, 476, 712, 1174, 1633
DraI TTTAAA 2 cut(s) 664, 868
EaeI YGGCCR 1 cut(s) 628
EciI GGCGGA 5 cut(s) 20, 90, 138, 144, 150
Eco130I CCWWGG 3 cut(s) 1357, 1638, 1668
Eco31I GGTCTC 1 cut(s) 1515
Eco32I GATATC 1 cut(s) 243
Eco47I GGWCC 2 cut(s) 767, 1139
Eco57I CTGAAG 1 cut(s) 351
Eco88I CYCGRG 1 cut(s) 1742
EcoO109I RGGNCCY 2 cut(s) 767, 1565
EcoRI GAATTC 1 cut(s) 431
EcoRII CCWGG 2 cut(s) 442, 1553
EcoRV GATATC 1 cut(s) 243
EcoT14I CCWWGG 3 cut(s) 1357, 1638, 1668
EcoT22I ATGCAT 3 cut(s) 539, 948, 1309
ErhI CCWWGG 3 cut(s) 1357, 1638, 1668
FaqI GGGAC 3 cut(s) 1013, 1070, 1152
FauI CCCGC 1 cut(s) 77
FauNDI CATATG 2 cut(s) 901, 1100
Fnu4HI GCNGC 5 cut(s) 166, 342, 590, 1473, 1488
FokI GGATG 3 cut(s) 461, 887, 1700
Fsp4HI GCNGC 5 cut(s) 166, 342, 590, 1473, 1488
FspBI CTAG 3 cut(s) 117, 644, 1208
GluI GCNGC 5 cut(s) 166, 342, 590, 1473, 1488
GsaI CCCAGC 2 cut(s) 899, 1351
HaeIII GGCC 5 cut(s) 262, 630, 1356, 1566, 1712
HapII CCGG 3 cut(s) 16, 47, 152
HincII GTYRAC 1 cut(s) 973
HindII GTYRAC 1 cut(s) 973
HpaII CCGG 3 cut(s) 16, 47, 152
HphI GGTGA 5 cut(s) 166, 237, 722, 1241, 1654
Hpy166II GTNNAC 2 cut(s) 973, 1064
Hpy188I TCNGA 8 cut(s) 28, 378, 775, 919, 1020, 1075, 1081, 1750
Hpy188III TCNNGA 6 cut(s) 192, 254, 515, 676, 1271, 1412
Hpy8I GTNNAC 2 cut(s) 973, 1064
Hpy99I CGWCG 1 cut(s) 1304
HpyAV CCTTC 7 cut(s) 780, 1138, 1271, 1372, 1540, 1639, 1723
HpyCH4III ACNGT 2 cut(s) 409, 427
HpyCH4IV ACGT 1 cut(s) 613
HpyF10VI GCNNNNNNNGC 6 cut(s) 81, 174, 884, 1353, 1577, 1709
HpyF3I CTNAG 2 cut(s) 918, 1749
HpySE526I ACGT 1 cut(s) 613
Kzo9I GATC 6 cut(s) 10, 296, 476, 712, 1174, 1633
LmnI GCTCC 4 cut(s) 154, 1076, 1648, 1700
Lsp1109I GCAGC 4 cut(s) 353, 601, 1459, 1474
LweI GCATC 8 cut(s) 508, 865, 1190, 1294, 1341, 1456, 1603, 1732
MaeI CTAG 3 cut(s) 117, 644, 1208
MaeII ACGT 1 cut(s) 613
MaeIII GTNAC 5 cut(s) 142, 791, 1045, 1229, 1693
MalI GATC 6 cut(s) 12, 298, 478, 714, 1176, 1635
MboI GATC 6 cut(s) 10, 296, 476, 712, 1174, 1633
MboII GAAGA 4 cut(s) 1033, 1718, 1789, 1819
MfeI CAATTG 2 cut(s) 603, 1503
MflI RGATCY 1 cut(s) 476
MhlI GDGCHC 1 cut(s) 314
MlsI TGGCCA 1 cut(s) 630
MluI ACGCGT 1 cut(s) 575
MluNI TGGCCA 1 cut(s) 630
MlyI GAGTC 6 cut(s) 244, 968, 1085, 1114, 1754, 1772
MmeI TCCRAC 5 cut(s) 81, 270, 454, 550, 1520
Mox20I TGGCCA 1 cut(s) 630
Mph1103I ATGCAT 3 cut(s) 539, 948, 1309
MscI TGGCCA 1 cut(s) 630
MslI CAYNNNNRTG 1 cut(s) 1455
Msp20I TGGCCA 1 cut(s) 630
MspI CCGG 3 cut(s) 16, 47, 152
MspR9I CCNGG 3 cut(s) 48, 444, 1555
MunI CAATTG 2 cut(s) 603, 1503
MvaI CCWGG 2 cut(s) 444, 1555
MvnI CGCG 1 cut(s) 577
MwoI GCNNNNNNNGC 6 cut(s) 81, 174, 884, 1353, 1577, 1709
NciI CCSGG 1 cut(s) 48
NdeI CATATG 2 cut(s) 901, 1100
NdeII GATC 6 cut(s) 10, 296, 476, 712, 1174, 1633
NlaIV GGNNCC 3 cut(s) 478, 1072, 1140
NmuCI GTSAC 3 cut(s) 1045, 1229, 1693
NsiI ATGCAT 3 cut(s) 539, 948, 1309
NspI RCATGY 3 cut(s) 1494, 1553, 1630
NspV TTCGAA 1 cut(s) 215
PagI TCATGA 2 cut(s) 253, 1411
PfeI GAWTC 5 cut(s) 188, 455, 778, 851, 1382
PkrI GCNGC 5 cut(s) 167, 343, 591, 1474, 1489
PleI GAGTC 6 cut(s) 244, 968, 1084, 1113, 1753, 1771
PpsI GAGTC 6 cut(s) 244, 968, 1084, 1113, 1753, 1771
PpuMI RGGWCCY 1 cut(s) 767
PshBI ATTAAT 1 cut(s) 1008
Psp1406I AACGTT 1 cut(s) 613
Psp5II RGGWCCY 1 cut(s) 767
Psp6I CCWGG 2 cut(s) 442, 1553
PspFI CCCAGC 2 cut(s) 895, 1347
PspGI CCWGG 2 cut(s) 442, 1553
PspN4I GGNNCC 3 cut(s) 478, 1072, 1140
PspPI GGNCC 3 cut(s) 767, 1139, 1565
PspPPI RGGWCCY 1 cut(s) 767
PsuI RGATCY 1 cut(s) 476
RsaI GTAC 2 cut(s) 493, 1548
RsaNI GTAC 2 cut(s) 492, 1547
RseI CAYNNNNRTG 1 cut(s) 1455
SatI GCNGC 5 cut(s) 166, 342, 590, 1473, 1488
Sau3AI GATC 6 cut(s) 10, 296, 476, 712, 1174, 1633
Sau96I GGNCC 3 cut(s) 767, 1139, 1565
SchI GAGTC 6 cut(s) 244, 968, 1085, 1114, 1754, 1772
ScrFI CCNGG 3 cut(s) 48, 444, 1555
SduI GDGCHC 1 cut(s) 314
SfaNI GCATC 8 cut(s) 508, 865, 1190, 1294, 1341, 1456, 1603, 1732
SfcI CTRYAG 4 cut(s) 36, 180, 205, 426
SfuI TTCGAA 1 cut(s) 215
SinI GGWCC 2 cut(s) 767, 1139
SmiMI CAYNNNNRTG 1 cut(s) 1455
SmlI CTYRAG 1 cut(s) 1839
SmoI CTYRAG 1 cut(s) 1839
SpeI ACTAGT 1 cut(s) 1207
SspMI CTAG 3 cut(s) 117, 644, 1208
StyD4I CCNGG 3 cut(s) 46, 442, 1553
StyI CCWWGG 3 cut(s) 1357, 1638, 1668
TaaI ACNGT 2 cut(s) 409, 427
TaiI ACGT 1 cut(s) 616
TaqI TCGA 5 cut(s) 215, 435, 1302, 1769, 1805
TaqII GACCGA 1 cut(s) 1156
TatI WGTACW 1 cut(s) 1546
TauI GCSGC 1 cut(s) 168
TfiI GAWTC 5 cut(s) 188, 455, 778, 851, 1382
TscAI CASTG 3 cut(s) 240, 487, 1446
TseFI GTSAC 3 cut(s) 1045, 1229, 1693
TseI GCWGC 4 cut(s) 341, 589, 1472, 1487
Tsp45I GTSAC 3 cut(s) 1045, 1229, 1693
TspDTI ATGAA 2 cut(s) 270, 1206
TspGWI ACGGA 3 cut(s) 123, 320, 435
TspRI CASTG 3 cut(s) 240, 487, 1446
VpaK11BI GGWCC 2 cut(s) 767, 1139
VspI ATTAAT 1 cut(s) 1008
XapI RAATTY 2 cut(s) 431, 658
XceI RCATGY 3 cut(s) 1494, 1553, 1630
XcmI CCANNNNNNNNNTGG 2 cut(s) 1212, 1393
XspI CTAG 3 cut(s) 117, 644, 1208
Zsp2I ATGCAT 3 cut(s) 539, 948, 1309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.