Rh6AG283800

Sterol 3-beta-glucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0001484
Physical Location & Seq
Forward (+)
47928046 .. 47936024
7979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG283800.1

Sequence Viewer

Length: 2139 bp
ATGAAGCACACCATCATCACTCATGCCCCTCCATCACCACCCACTTTTATCGAACGTGCCCGTCTCTTCCGGACAGAATCCAATTCTCTCTTTCTTTTTCTTCTCCGATTCTCTCTCTCCCTCTCCCTCTCCCTCGCCCTCTCCGATTCTCAATTTCGAATCTTACAGCGTTCTCAGAATCCAAGCCACGCTCTTCTTCTTCTTCTCGCTTCCGATCTGGATCAGGACCGGCCGCGCGGTCCGGAGTGTGATCGGGAACAGGCGGCGGTGATGGCGGAGGATCGCCGGAGTAGTTCCGAGAGTTCTGTAGAAGTTCCGGGGAGAATTGAGCAGGAAAGTGACGGCGGAAGTGGCGGGAAAAGAATAGAGTTGGAGAACGGAGAGGCTAGTGGCGGAGGCGTTACTGCTCCGGTGAATAACGGCGGCGGCAGTAGCTCTACAGGGAATTCAGGAAGTTCTGGCTTTAGCTTTTCGAAAACGAACGCATTGCCAGTGGAGATATCACCGACTCATGAAAGGCCAGAACCACATTCAGTTCCAGCAAAGTTGGAGAGATCAAAAACGGAGAGCACCAGACCTAATGTATTGTCCAAAGAGGCAGCAAAGATTTTTGACGACAAAACTTCAGTCCATCAGAAGTTAAAGCTGTTAAATAGAATAGCCACTGTGAAAGATGACGGAACTGTAGAATTCGATGTTCCAGGGGATGTTGAATCTGAATCTTTTGGTGTTGGATCCACTGCTGGTTGTACCGATGTTGCTGATGAGCCTCTTGATGCAACAGAGCTCCAATGCATACCACCAATGCAAATTGTAATGCTTATTGTTGGAACGCGTGGAGATGTGCAGCCGTTTATTGCAATTGGCAAGCGTTTGCAGGACTATGGCCATCGTGTAAGACTAGCTACTCATTCAAATTTTAAAGAGTTTGTCTTGACTGCTGGTTTGGAGTTCTACCCTTTAGGCGGTGATCCAAAAGTTCTTGCTGGCTATATGGTTAAAAATAAAGGCTTCTTGCCATCAGGGCCTTCGGAGATTCCTATTCAGCGTAACCAAATAAAGGAAATTATATATTCCTTACTTCCGGCTTGCAAAGAGCCTGATGTAGATTCTGGTATCCCCTTTAAAGCGGATGCAATTATTGCAAACCCCCCAGCATATGGGCATACTCATGTGGCGGAGGCACTGAAAATACCTCTTCATATATTTTTTACAATGCCATGGACGCCAACAAGTGAATTTCCACATCCCTTGTCCCGCGTTAAACAACAAACTGGATATAGACTATCGTATCAAATTGTTGACTCCTTAATATGGCTTGGAATACGGGACATGATTAATGATGTTCGGAAGAAAAAATTGAGATTACGACCTGTCACATATTTAAGTGGTTCACAAGGCTCTGAGTCTGATGTCCCACATGGATACATATGGAGTCCTCATCTTGTTCCCAAACCAAAAGATTGGGGACCTAAGGTTGATGTGGTGGGATTTTGCTTCCTTGATCTTGCATCAAATTATGAACCTCCACAACCACTAGTGGAATGGCTTAAAGCTGGTGACAAGCCTATATACATTGGGTTTGGTAGCCTTCCTGTTCAAGAGCCAGAGAAAATGACCCAAATAATCGTCGATGCATTAGAAAAGACAGGACAGAGAGGCATCATTAACAAAGGCTGGGGTGGCCTTGGCAACTTGGCTGAACCAAAGGAATCCATATACTTATTGGACAACTGCCCTCATGACTGGCTGTTTCTACAATGCAAGGCAGTGGTGCATCATGGAGGTGCTGGAACAACGGCTGCTGGTCTAAAAGCGGCATGTCCGACAACAATTGTTCCTTTCTTTGGAGACCAACCCTTCTGGGGAGAGCGAGTACATGCCAGGGGAGTAGGCCCTGCACCCATTGCAGTTGATGAGTTCTCACTTCCCAAATTGGTTGATGCTATAAATTTCATGCTTGATCCCAAGGTGAAGGAGCGTGCTATTCAACTTGCCAAGGATATGGAGAATGAGGATGGTGTGACAGGAGCAGTCATGGCCTTCTTTAAGCATCTTCCTCGCAAAAAGCCCGAGTCTGAGCCAGAAACAGAGTCATCGAGCCTTTGCTCTATAAGTAGATGTTTTGGTTGTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_transf_28 UDPGT

Protein Analysis

712

Amino Acids

77.75

Weight (kDa)

6.06

Isoelectric Point (pI)

44.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_28 PF03033 271 - 414 6.5e-35 Glycosyltransferase family 28 N-terminal domain
UDPGT PF00201 520 - 666 5.8e-06 UDP-glucoronosyl and UDP-glucosyl transferase
EryCIII-like_C PF06722 572 - 671 1.4e-11 Erythromycin biosynthesis protein CIII-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1160
AccII CGCG 4 cut(s) 235, 237, 835, 1260
AccIII TCCGGA 2 cut(s) 69, 241
AclWI GGATC 6 cut(s) 228, 288, 729, 742, 965, 1958
AcoI YGGCCR 2 cut(s) 230, 886
AcsI RAATTY 5 cut(s) 445, 689, 916, 1238, 1951
AcuI CTGAAG 1 cut(s) 609
AcyI GRCGYC 1 cut(s) 1226
AfaI GTAC 2 cut(s) 751, 1878
AfiI CCNNNNNNNGG 6 cut(s) 965, 1060, 1160, 1314, 1847, 1865
AflIII ACRYGT 1 cut(s) 833
AgsI TTSAA 4 cut(s) 713, 915, 1601, 1991
AhlI ACTAGT 1 cut(s) 1537
AjnI CCWGG 2 cut(s) 700, 1883
AluBI AGCT 6 cut(s) 435, 468, 646, 787, 905, 1556
AluI AGCT 6 cut(s) 435, 468, 646, 787, 905, 1556
Alw21I GWGCWC 2 cut(s) 572, 789
Alw26I GTCTC 2 cut(s) 68, 1845
AlwI GGATC 6 cut(s) 228, 288, 729, 742, 965, 1958
Ama87I CYCGRG 1 cut(s) 2072
Aor13HI TCCGGA 2 cut(s) 69, 241
AoxI GGCC 7 cut(s) 230, 518, 886, 1025, 1684, 1894, 2040
ApeKI GCWGC 3 cut(s) 599, 847, 1802
ApoI RAATTY 5 cut(s) 445, 689, 916, 1238, 1951
ArsI GACNNNNNNTTYG 4 cut(s) 612, 644, 928, 960
AseI ATTAAT 1 cut(s) 1338
AspLEI GCGC 1 cut(s) 237
AspS9I GGNCC 5 cut(s) 226, 239, 1025, 1469, 1895
AsuC2I CCSGG 1 cut(s) 318
AsuHPI GGTGA 7 cut(s) 27, 280, 424, 495, 980, 1571, 1984
AsuII TTCGAA 2 cut(s) 157, 473
AvaI CYCGRG 1 cut(s) 2072
AvaII GGWCC 3 cut(s) 226, 239, 1469
AxyI CCTNAGG 1 cut(s) 1473
BaeGI GKGCMC 1 cut(s) 61
BaeI ACNNNNGTAYC 2 cut(s) 1274, 1307
BalI TGGCCA 1 cut(s) 888
BamHI GGATCC 1 cut(s) 734
BanII GRGCYC 1 cut(s) 789
Bbv12I GWGCWC 2 cut(s) 572, 789
BbvI GCAGC 3 cut(s) 611, 859, 1789
BccI CCATC 7 cut(s) 20, 40, 265, 639, 897, 1027, 2012
BceAI ACGGC 4 cut(s) 358, 436, 835, 1815
BcgI CGANNNNNNTGC 4 cut(s) 469, 503, 2042, 2076
BciT130I CCWGG 2 cut(s) 702, 1885
BciVI GTATCC 2 cut(s) 1127, 1418
BcnI CCSGG 1 cut(s) 318
BcoDI GTCTC 2 cut(s) 68, 1845
BcuI ACTAGT 1 cut(s) 1537
BfaI CTAG 3 cut(s) 387, 902, 1538
BfmI CTRYAG 3 cut(s) 306, 438, 684
BfuI GTATCC 2 cut(s) 1127, 1418
BglI GCCNNNNNGGC 1 cut(s) 1024
BisI GCNGC 8 cut(s) 233, 264, 424, 427, 600, 848, 1803, 1818
BlsI GCNGC 8 cut(s) 234, 265, 425, 428, 601, 849, 1804, 1819
Bme1390I CCNGG 3 cut(s) 318, 702, 1885
Bme18I GGWCC 3 cut(s) 226, 239, 1469
BmeT110I CYCGRG 1 cut(s) 2072
BmgT120I GGNCC 5 cut(s) 226, 239, 1025, 1469, 1895
BmiI GGNNCC 2 cut(s) 736, 1470
BmrFI CCNGG 3 cut(s) 318, 702, 1885
BmsI GCATC 8 cut(s) 766, 1123, 1520, 1624, 1671, 1786, 1933, 2062
Bpu14I TTCGAA 2 cut(s) 157, 473
BpuMI CCSGG 1 cut(s) 318
BsaBI GATNNNNATC 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 1226
BsaI GGTCTC 1 cut(s) 1845
BsaJI CCNNGG 7 cut(s) 317, 701, 1220, 1687, 1884, 1968, 1998
BsaWI WCCGGW 3 cut(s) 69, 241, 409
BsaXI ACNNNNNCTCC 2 cut(s) 1860, 1890
Bsc4I CCNNNNNNNGG 6 cut(s) 965, 1060, 1160, 1314, 1847, 1865
Bse118I RCCGGY 1 cut(s) 228
Bse1I ACTGG 3 cut(s) 491, 1279, 1751
Bse21I CCTNAGG 1 cut(s) 1473
Bse3DI GCAATG 2 cut(s) 485, 1905
Bse8I GATNNNNATC 1 cut(s) 219
BseAI TCCGGA 2 cut(s) 69, 241
BseBI CCWGG 2 cut(s) 702, 1885
BseDI CCNNGG 7 cut(s) 317, 701, 1220, 1687, 1884, 1968, 1998
BseGI GGATG 4 cut(s) 712, 1138, 1246, 2023
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 6 cut(s) 965, 1060, 1160, 1314, 1847, 1865
BseMI GCAATG 2 cut(s) 485, 1905
BseMII CTCAG 3 cut(s) 188, 1395, 2070
BseNI ACTGG 3 cut(s) 491, 1279, 1751
BseSI GKGCMC 1 cut(s) 61
BseX3I CGGCCG 1 cut(s) 230
BseXI GCAGC 3 cut(s) 611, 859, 1789
BseYI CCCAGC 2 cut(s) 1153, 1677
BsgI GTGCAG 2 cut(s) 866, 1884
Bsh1236I CGCG 4 cut(s) 235, 237, 835, 1260
Bsh1285I CGRYCG 1 cut(s) 233
BshFI GGCC 7 cut(s) 232, 520, 888, 1027, 1686, 1896, 2042
BsiEI CGRYCG 1 cut(s) 233
BsiHKAI GWGCWC 2 cut(s) 572, 789
BsiHKCI CYCGRG 1 cut(s) 2072
BsiSI CCGG 7 cut(s) 70, 229, 242, 286, 317, 410, 1085
BslFI GGGAC 4 cut(s) 1240, 1343, 1400, 1482
BslI CCNNNNNNNGG 6 cut(s) 965, 1060, 1160, 1314, 1847, 1865
BsmAI GTCTC 2 cut(s) 68, 1845
BsmBI CGTCTC 1 cut(s) 68
BsmFI GGGAC 4 cut(s) 1240, 1343, 1400, 1482
BsnI GGCC 7 cut(s) 232, 520, 888, 1027, 1686, 1896, 2042
Bso31I GGTCTC 1 cut(s) 1845
BsoBI CYCGRG 1 cut(s) 2072
Bsp119I TTCGAA 2 cut(s) 157, 473
Bsp1286I GDGCHC 3 cut(s) 61, 572, 789
Bsp13I TCCGGA 2 cut(s) 69, 241
Bsp143I GATC 9 cut(s) 214, 220, 250, 280, 554, 734, 970, 1504, 1963
Bsp19I CCATGG 1 cut(s) 1220
BspANI GGCC 7 cut(s) 232, 520, 888, 1027, 1686, 1896, 2042
BspCNI CTCAG 3 cut(s) 187, 1396, 2071
BspEI TCCGGA 2 cut(s) 69, 241
BspFNI CGCG 4 cut(s) 235, 237, 835, 1260
BspHI TCATGA 2 cut(s) 511, 1741
BspLI GGNNCC 2 cut(s) 736, 1470
BspPI GGATC 6 cut(s) 228, 288, 729, 742, 965, 1958
BspQI GCTCTTC 1 cut(s) 198
BspT104I TTCGAA 2 cut(s) 157, 473
BspTNI GGTCTC 1 cut(s) 1845
BsrDI GCAATG 2 cut(s) 485, 1905
BsrFI RCCGGY 1 cut(s) 228
BsrI ACTGG 3 cut(s) 491, 1279, 1751
BssAI RCCGGY 1 cut(s) 228
BssECI CCNNGG 7 cut(s) 317, 701, 1220, 1687, 1884, 1968, 1998
BssMI GATC 9 cut(s) 214, 220, 250, 280, 554, 734, 970, 1504, 1963
BssNI GRCGYC 1 cut(s) 1226
BssT1I CCWWGG 4 cut(s) 1220, 1687, 1968, 1998
Bst2UI CCWGG 2 cut(s) 702, 1885
Bst4CI ACNGT 2 cut(s) 667, 685
Bst6I CTCTTC 3 cut(s) 71, 198, 1203
BstACI GRCGYC 1 cut(s) 1226
BstAPI GCANNNNNTGC 2 cut(s) 1142, 1907
BstBI TTCGAA 2 cut(s) 157, 473
BstC8I GCNNGC 4 cut(s) 869, 988, 1090, 1983
BstDEI CTNAG 4 cut(s) 174, 1404, 1473, 2079
BstDSI CCRYGG 1 cut(s) 1220
BstF5I GGATG 4 cut(s) 712, 1138, 1246, 2023
BstFNI CGCG 4 cut(s) 235, 237, 835, 1260
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 9 cut(s) 217, 223, 253, 283, 557, 737, 973, 1507, 1966
BstMAI GTCTC 2 cut(s) 68, 1845
BstMBI GATC 9 cut(s) 214, 220, 250, 280, 554, 734, 970, 1504, 1963
BstMCI CGRYCG 1 cut(s) 233
BstMWI GCNNNNNNNGC 9 cut(s) 272, 351, 432, 1024, 1142, 1225, 1683, 1907, 2039
BstNI CCWGG 2 cut(s) 702, 1885
BstNSI RCATGY 2 cut(s) 1824, 1883
BstSCI CCNGG 3 cut(s) 316, 700, 1883
BstSFI CTRYAG 3 cut(s) 306, 438, 684
BstSLI GKGCMC 1 cut(s) 61
BstUI CGCG 4 cut(s) 235, 237, 835, 1260
BstV1I GCAGC 3 cut(s) 611, 859, 1789
BstX2I RGATCY 1 cut(s) 734
BstXI CCANNNNNNTGG 2 cut(s) 1464, 2005
BstYI RGATCY 1 cut(s) 734
BstZI CGGCCG 1 cut(s) 230
Bsu36I CCTNAGG 1 cut(s) 1473
BsuI GTATCC 2 cut(s) 1127, 1418
BsuRI GGCC 7 cut(s) 232, 520, 888, 1027, 1686, 1896, 2042
BtgI CCRYGG 1 cut(s) 1220
BtsCI GGATG 4 cut(s) 712, 1138, 1246, 2023
BtsI GCAGTG 2 cut(s) 738, 1776
BtsIMutI CAGTG 5 cut(s) 498, 663, 738, 1184, 1776
Cac8I GCNNGC 4 cut(s) 869, 988, 1090, 1983
CciI TCATGA 2 cut(s) 511, 1741
CfoI GCGC 1 cut(s) 237
Cfr10I RCCGGY 1 cut(s) 228
Cfr13I GGNCC 5 cut(s) 226, 239, 1025, 1469, 1895
CpoI CGGWCCG 1 cut(s) 239
CseI GACGC 1 cut(s) 1234
Csp6I GTAC 2 cut(s) 750, 1877
CspCI CAANNNNNGTGG 2 cut(s) 1233, 1268
CspI CGGWCCG 1 cut(s) 239
CviQI GTAC 2 cut(s) 750, 1877
DdeI CTNAG 4 cut(s) 174, 1404, 1473, 2079
DpnI GATC 9 cut(s) 216, 222, 252, 282, 556, 736, 972, 1506, 1965
DpnII GATC 9 cut(s) 214, 220, 250, 280, 554, 734, 970, 1504, 1963
DraI TTTAAA 2 cut(s) 922, 1126
EaeI YGGCCR 2 cut(s) 230, 886
EagI CGGCCG 1 cut(s) 230
Eam1104I CTCTTC 3 cut(s) 71, 198, 1203
EarI CTCTTC 3 cut(s) 71, 198, 1203
EciI GGCGGA 4 cut(s) 290, 360, 408, 1193
Ecl136II GAGCTC 1 cut(s) 787
EclXI CGGCCG 1 cut(s) 230
Eco130I CCWWGG 4 cut(s) 1220, 1687, 1968, 1998
Eco24I GRGCYC 1 cut(s) 789
Eco31I GGTCTC 1 cut(s) 1845
Eco32I GATATC 1 cut(s) 501
Eco47I GGWCC 3 cut(s) 226, 239, 1469
Eco52I CGGCCG 1 cut(s) 230
Eco53kI GAGCTC 1 cut(s) 787
Eco57I CTGAAG 1 cut(s) 609
Eco81I CCTNAGG 1 cut(s) 1473
Eco88I CYCGRG 1 cut(s) 2072
EcoICRI GAGCTC 1 cut(s) 787
EcoO109I RGGNCCY 3 cut(s) 1025, 1469, 1895
EcoRI GAATTC 2 cut(s) 445, 689
EcoRII CCWGG 2 cut(s) 700, 1883
EcoRV GATATC 1 cut(s) 501
EcoT14I CCWWGG 4 cut(s) 1220, 1687, 1968, 1998
EcoT22I ATGCAT 2 cut(s) 797, 1639
EcoT38I GRGCYC 1 cut(s) 789
ErhI CCWWGG 4 cut(s) 1220, 1687, 1968, 1998
Esp3I CGTCTC 1 cut(s) 68
FaqI GGGAC 4 cut(s) 1240, 1343, 1400, 1482
FauI CCCGC 2 cut(s) 347, 1265
FauNDI CATATG 2 cut(s) 1159, 1430
Fnu4HI GCNGC 8 cut(s) 233, 264, 424, 427, 600, 848, 1803, 1818
FokI GGATG 4 cut(s) 719, 1145, 1233, 2030
FriOI GRGCYC 1 cut(s) 789
Fsp4HI GCNGC 8 cut(s) 233, 264, 424, 427, 600, 848, 1803, 1818
FspBI CTAG 3 cut(s) 387, 902, 1538
GlaI GCGC 1 cut(s) 236
GluI GCNGC 8 cut(s) 233, 264, 424, 427, 600, 848, 1803, 1818
GsaI CCCAGC 2 cut(s) 1157, 1681
HaeIII GGCC 7 cut(s) 232, 520, 888, 1027, 1686, 1896, 2042
HapII CCGG 7 cut(s) 70, 229, 242, 286, 317, 410, 1085
HgaI GACGC 1 cut(s) 1234
HhaI GCGC 1 cut(s) 237
Hin1I GRCGYC 1 cut(s) 1226
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HincII GTYRAC 1 cut(s) 1303
HindII GTYRAC 1 cut(s) 1303
HpaII CCGG 7 cut(s) 70, 229, 242, 286, 317, 410, 1085
HphI GGTGA 7 cut(s) 27, 280, 424, 495, 980, 1571, 1984
Hpy166II GTNNAC 2 cut(s) 1303, 1394
Hpy8I GTNNAC 2 cut(s) 1303, 1394
Hpy99I CGWCG 1 cut(s) 1634
HpyAV CCTTC 5 cut(s) 1038, 1601, 1870, 1969, 2053
HpyCH4III ACNGT 2 cut(s) 667, 685
HpyCH4IV ACGT 1 cut(s) 55
HpyF10VI GCNNNNNNNGC 9 cut(s) 272, 351, 432, 1024, 1142, 1225, 1683, 1907, 2039
HpyF3I CTNAG 4 cut(s) 174, 1404, 1473, 2079
HpySE526I ACGT 1 cut(s) 55
Hsp92I GRCGYC 1 cut(s) 1226
HspAI GCGC 1 cut(s) 235
Kpn2I TCCGGA 2 cut(s) 69, 241
Kzo9I GATC 9 cut(s) 214, 220, 250, 280, 554, 734, 970, 1504, 1963
LguI GCTCTTC 1 cut(s) 198
LmnI GCTCC 4 cut(s) 412, 792, 1978, 2030
Lsp1109I GCAGC 3 cut(s) 611, 859, 1789
LweI GCATC 8 cut(s) 766, 1123, 1520, 1624, 1671, 1786, 1933, 2062
MaeI CTAG 3 cut(s) 387, 902, 1538
MaeII ACGT 1 cut(s) 55
MaeIII GTNAC 6 cut(s) 338, 400, 1049, 1375, 1559, 2023
MalI GATC 9 cut(s) 216, 222, 252, 282, 556, 736, 972, 1506, 1965
MboI GATC 9 cut(s) 214, 220, 250, 280, 554, 734, 970, 1504, 1963
MboII GAAGA 9 cut(s) 58, 92, 185, 188, 191, 194, 1190, 1363, 2048
MfeI CAATTG 2 cut(s) 861, 1833
MflI RGATCY 1 cut(s) 734
MhlI GDGCHC 3 cut(s) 61, 572, 789
MlsI TGGCCA 1 cut(s) 888
MluI ACGCGT 1 cut(s) 833
MluNI TGGCCA 1 cut(s) 888
MlyI GAGTC 6 cut(s) 502, 1298, 1415, 1444, 2084, 2102
MmeI TCCRAC 5 cut(s) 351, 528, 712, 808, 1850
Mox20I TGGCCA 1 cut(s) 888
Mph1103I ATGCAT 2 cut(s) 797, 1639
MroI TCCGGA 2 cut(s) 69, 241
MscI TGGCCA 1 cut(s) 888
MslI CAYNNNNRTG 2 cut(s) 1170, 1785
Msp20I TGGCCA 1 cut(s) 888
MspI CCGG 7 cut(s) 70, 229, 242, 286, 317, 410, 1085
MspR9I CCNGG 3 cut(s) 318, 702, 1885
MunI CAATTG 2 cut(s) 861, 1833
MvaI CCWGG 2 cut(s) 702, 1885
MvnI CGCG 4 cut(s) 235, 237, 835, 1260
MwoI GCNNNNNNNGC 9 cut(s) 272, 351, 432, 1024, 1142, 1225, 1683, 1907, 2039
NciI CCSGG 1 cut(s) 318
NcoI CCATGG 1 cut(s) 1220
NdeI CATATG 2 cut(s) 1159, 1430
NdeII GATC 9 cut(s) 214, 220, 250, 280, 554, 734, 970, 1504, 1963
NlaIV GGNNCC 2 cut(s) 736, 1470
NmuCI GTSAC 4 cut(s) 338, 1375, 1559, 2023
NsiI ATGCAT 2 cut(s) 797, 1639
NspI RCATGY 2 cut(s) 1824, 1883
NspV TTCGAA 2 cut(s) 157, 473
PagI TCATGA 2 cut(s) 511, 1741
PciSI GCTCTTC 1 cut(s) 198
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PflMI CCANNNNNTGG 1 cut(s) 1160
PkrI GCNGC 8 cut(s) 234, 265, 425, 428, 601, 849, 1804, 1819
PleI GAGTC 6 cut(s) 502, 1298, 1414, 1443, 2083, 2101
PpsI GAGTC 6 cut(s) 502, 1298, 1414, 1443, 2083, 2101
PpuMI RGGWCCY 1 cut(s) 1469
PshBI ATTAAT 1 cut(s) 1338
Psp124BI GAGCTC 1 cut(s) 789
Psp5II RGGWCCY 1 cut(s) 1469
Psp6I CCWGG 2 cut(s) 700, 1883
PspFI CCCAGC 2 cut(s) 1153, 1677
PspGI CCWGG 2 cut(s) 700, 1883
PspN4I GGNNCC 2 cut(s) 736, 1470
PspPI GGNCC 5 cut(s) 226, 239, 1025, 1469, 1895
PspPPI RGGWCCY 1 cut(s) 1469
PsuI RGATCY 1 cut(s) 734
RsaI GTAC 2 cut(s) 751, 1878
RsaNI GTAC 2 cut(s) 750, 1877
RseI CAYNNNNRTG 2 cut(s) 1170, 1785
Rsr2I CGGWCCG 1 cut(s) 239
RsrII CGGWCCG 1 cut(s) 239
SacI GAGCTC 1 cut(s) 789
SapI GCTCTTC 1 cut(s) 198
SatI GCNGC 8 cut(s) 233, 264, 424, 427, 600, 848, 1803, 1818
Sau3AI GATC 9 cut(s) 214, 220, 250, 280, 554, 734, 970, 1504, 1963
Sau96I GGNCC 5 cut(s) 226, 239, 1025, 1469, 1895
SchI GAGTC 6 cut(s) 502, 1298, 1415, 1444, 2084, 2102
ScrFI CCNGG 3 cut(s) 318, 702, 1885
SduI GDGCHC 3 cut(s) 61, 572, 789
SfaNI GCATC 8 cut(s) 766, 1123, 1520, 1624, 1671, 1786, 1933, 2062
SfcI CTRYAG 3 cut(s) 306, 438, 684
SfuI TTCGAA 2 cut(s) 157, 473
SinI GGWCC 3 cut(s) 226, 239, 1469
SmiMI CAYNNNNRTG 2 cut(s) 1170, 1785
SpeI ACTAGT 1 cut(s) 1537
SspMI CTAG 3 cut(s) 387, 902, 1538
SstI GAGCTC 1 cut(s) 789
StyD4I CCNGG 3 cut(s) 316, 700, 1883
StyI CCWWGG 4 cut(s) 1220, 1687, 1968, 1998
TaaI ACNGT 2 cut(s) 667, 685
TaiI ACGT 1 cut(s) 58
TaqI TCGA 6 cut(s) 51, 157, 473, 693, 1632, 2099
TatI WGTACW 1 cut(s) 1876
TauI GCSGC 5 cut(s) 235, 266, 426, 429, 1820
TscAI CASTG 5 cut(s) 498, 670, 745, 1191, 1776
TseFI GTSAC 4 cut(s) 338, 1375, 1559, 2023
TseI GCWGC 3 cut(s) 599, 847, 1802
Tsp45I GTSAC 4 cut(s) 338, 1375, 1559, 2023
TspDTI ATGAA 5 cut(s) 17, 528, 1190, 1536, 1945
TspGWI ACGGA 3 cut(s) 393, 578, 693
TspRI CASTG 5 cut(s) 498, 670, 745, 1191, 1776
Van91I CCANNNNNTGG 1 cut(s) 1160
VpaK11BI GGWCC 3 cut(s) 226, 239, 1469
VspI ATTAAT 1 cut(s) 1338
XapI RAATTY 5 cut(s) 445, 689, 916, 1238, 1951
XceI RCATGY 2 cut(s) 1824, 1883
XcmI CCANNNNNNNNNTGG 2 cut(s) 1542, 1723
XspI CTAG 3 cut(s) 387, 902, 1538
Zsp2I ATGCAT 2 cut(s) 797, 1639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.