Rroxscaffold_7G00183400

Sterol 3-beta-glucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001484
Physical Location & Seq
Reverse (-)
22488603 .. 22496346
7744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00183400.1

Sequence Viewer

Length: 966 bp
ATGGCGGAGGATCGCCGGAGTAGTTCCGAGAGTTCTGTAGAAGTTCCGGGGAGAATTGAGCAGGAAATTGACGGCGGAAGTGGCGGGAAAAGAATAGAGTTGGAGAACGGAGAGGCTAGTGGCGGAGGCGGAGGCGTTACTGCTCCGGTGAATAACGGCGGCGGTAGTAGCTCTTCAGGGAATTCAGGAAGTTCTGGCTTTAACTTTTCGAAAACGAACGCATTGCCAGTGGAGATATCACCGACTCATGAAAGGCCAGAACCACATTCAGTTCCAGCAAAGTTGGAGAGATCAAAAACGGAGAGCACCAGACCTAATGTATTGTCCAAAGAGGCAGCAAAGATTTTTGACGACAAAACTTCAGTCCATCAGAAGTTAAAGCTGTTAAATAGAATTGCTACTGTGAAAGATGACGGAACTGTAGAATTCGATGTTCCAGGGGATGTTGAATCTCAATCTTTTGGTGTTGGATCCACTGCTGGTTGTACCGATGTTGCTGATGAGCCTCTTGATGCAACAGAGCTCCAATGCATACCACCCATGCAAATAGTAATGCTTATAGTTGGAACGCGTGGAGATGTGCAGCCGTTTATTGCAATTGGCAAACGTTTGCAGGACTATGGCCATCGTGTAAGACTAGCTACTCATTCAAATTTTAAAGAGTTTGTCTTGACTGCTGGTTTGGAGTTCTACCCTTTAGGCGGTGATCCAAAAGTTCTTGCTGGCTATATGGTTAAAAATAAAGGCTTCTTGCCGTCAGGTCCTTCGGAGATTCCTATTCAGCGTAACCAAATAAAGGAAATTATATATTCCTTACTTCCAGCTTGCAAAGAGCCTGATGTAGATTCTGGTATTCCCTTTAAAGCGGATGCAATTATTGCAAACCCCCCAGCATATGGGCATACTCATGTGGCGGAGTCACTGAAAATACCTCTTCATATATTTTTTACAATGCCATGGACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_transf_28 UDPGT

Protein Analysis

321

Amino Acids

34.26

Weight (kDa)

5.45

Isoelectric Point (pI)

53.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_28 PF03033 183 - 321 6.9e-34 Glycosyltransferase family 28 N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 896
AccII CGCG 1 cut(s) 571
AclI AACGTT 1 cut(s) 607
AclWI GGATC 4 cut(s) 18, 465, 478, 701
AcoI YGGCCR 1 cut(s) 622
AcsI RAATTY 3 cut(s) 181, 425, 652
AcuI CTGAAG 2 cut(s) 159, 345
AfaI GTAC 1 cut(s) 487
AfiI CCNNNNNNNGG 3 cut(s) 701, 796, 896
AflIII ACRYGT 1 cut(s) 569
AgsI TTSAA 2 cut(s) 449, 651
AjnI CCWGG 1 cut(s) 436
AluBI AGCT 5 cut(s) 171, 382, 523, 641, 824
AluI AGCT 5 cut(s) 171, 382, 523, 641, 824
Alw21I GWGCWC 2 cut(s) 308, 525
AlwI GGATC 4 cut(s) 18, 465, 478, 701
AoxI GGCC 2 cut(s) 254, 622
ApeKI GCWGC 2 cut(s) 335, 583
ApoI RAATTY 3 cut(s) 181, 425, 652
ArsI GACNNNNNNTTYG 4 cut(s) 348, 380, 664, 696
AspS9I GGNCC 1 cut(s) 761
AsuC2I CCSGG 1 cut(s) 48
AsuHPI GGTGA 3 cut(s) 160, 231, 716
AsuII TTCGAA 1 cut(s) 209
AvaII GGWCC 1 cut(s) 761
BalI TGGCCA 1 cut(s) 624
BamHI GGATCC 1 cut(s) 470
BanII GRGCYC 1 cut(s) 525
BarI GAAGNNNNNNTAC 2 cut(s) 157, 189
Bbv12I GWGCWC 2 cut(s) 308, 525
BbvI GCAGC 2 cut(s) 347, 595
BccI CCATC 2 cut(s) 375, 633
BceAI ACGGC 4 cut(s) 88, 172, 571, 739
BcgI CGANNNNNNTGC 2 cut(s) 205, 239
BciT130I CCWGG 1 cut(s) 438
BcnI CCSGG 1 cut(s) 48
BfaI CTAG 2 cut(s) 117, 638
BfmI CTRYAG 2 cut(s) 36, 420
BisI GCNGC 3 cut(s) 160, 336, 584
BlsI GCNGC 3 cut(s) 161, 337, 585
Bme1390I CCNGG 2 cut(s) 48, 438
Bme18I GGWCC 1 cut(s) 761
BmgT120I GGNCC 1 cut(s) 761
BmiI GGNNCC 1 cut(s) 472
BmrFI CCNGG 2 cut(s) 48, 438
BmsI GCATC 2 cut(s) 502, 859
Bpu14I TTCGAA 1 cut(s) 209
BpuMI CCSGG 1 cut(s) 48
BsaJI CCNNGG 3 cut(s) 47, 437, 956
BsaWI WCCGGW 1 cut(s) 145
Bsc4I CCNNNNNNNGG 3 cut(s) 701, 796, 896
Bse1I ACTGG 1 cut(s) 227
Bse3DI GCAATG 1 cut(s) 221
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 3 cut(s) 47, 437, 956
BseGI GGATG 2 cut(s) 448, 874
BseLI CCNNNNNNNGG 3 cut(s) 701, 796, 896
BseMI GCAATG 1 cut(s) 221
BseNI ACTGG 1 cut(s) 227
BseXI GCAGC 2 cut(s) 347, 595
BseYI CCCAGC 1 cut(s) 889
BsgI GTGCAG 1 cut(s) 602
Bsh1236I CGCG 1 cut(s) 571
BshFI GGCC 2 cut(s) 256, 624
BsiHKAI GWGCWC 2 cut(s) 308, 525
BsiSI CCGG 3 cut(s) 16, 47, 146
BslI CCNNNNNNNGG 3 cut(s) 701, 796, 896
BsnI GGCC 2 cut(s) 256, 624
Bsp119I TTCGAA 1 cut(s) 209
Bsp1286I GDGCHC 2 cut(s) 308, 525
Bsp143I GATC 4 cut(s) 10, 290, 470, 706
Bsp19I CCATGG 1 cut(s) 956
BspANI GGCC 2 cut(s) 256, 624
BspFNI CGCG 1 cut(s) 571
BspHI TCATGA 1 cut(s) 247
BspLI GGNNCC 1 cut(s) 472
BspPI GGATC 4 cut(s) 18, 465, 478, 701
BspQI GCTCTTC 1 cut(s) 178
BspT104I TTCGAA 1 cut(s) 209
BsrDI GCAATG 1 cut(s) 221
BsrI ACTGG 1 cut(s) 227
BssECI CCNNGG 3 cut(s) 47, 437, 956
BssMI GATC 4 cut(s) 10, 290, 470, 706
BssT1I CCWWGG 1 cut(s) 956
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 2 cut(s) 403, 421
Bst6I CTCTTC 2 cut(s) 178, 939
BstAPI GCANNNNNTGC 1 cut(s) 878
BstBI TTCGAA 1 cut(s) 209
BstC8I GCNNGC 2 cut(s) 724, 826
BstDSI CCRYGG 1 cut(s) 956
BstF5I GGATG 2 cut(s) 448, 874
BstFNI CGCG 1 cut(s) 571
BstKTI GATC 4 cut(s) 13, 293, 473, 709
BstMBI GATC 4 cut(s) 10, 290, 470, 706
BstMWI GCNNNNNNNGC 3 cut(s) 81, 168, 878
BstNI CCWGG 1 cut(s) 438
BstSCI CCNGG 2 cut(s) 46, 436
BstSFI CTRYAG 2 cut(s) 36, 420
BstUI CGCG 1 cut(s) 571
BstV1I GCAGC 2 cut(s) 347, 595
BstX2I RGATCY 1 cut(s) 470
BstYI RGATCY 1 cut(s) 470
BsuRI GGCC 2 cut(s) 256, 624
BtgI CCRYGG 1 cut(s) 956
BtsCI GGATG 2 cut(s) 448, 874
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 3 cut(s) 234, 474, 920
Cac8I GCNNGC 2 cut(s) 724, 826
CciI TCATGA 1 cut(s) 247
Cfr13I GGNCC 1 cut(s) 761
Csp6I GTAC 1 cut(s) 486
CviAII CATG 4 cut(s) 248, 541, 908, 957
CviQI GTAC 1 cut(s) 486
DpnI GATC 4 cut(s) 12, 292, 472, 708
DpnII GATC 4 cut(s) 10, 290, 470, 706
DraI TTTAAA 2 cut(s) 658, 862
EaeI YGGCCR 1 cut(s) 622
Eam1104I CTCTTC 2 cut(s) 178, 939
EarI CTCTTC 2 cut(s) 178, 939
EciI GGCGGA 5 cut(s) 20, 90, 138, 144, 929
Ecl136II GAGCTC 1 cut(s) 523
Eco130I CCWWGG 1 cut(s) 956
Eco24I GRGCYC 1 cut(s) 525
Eco32I GATATC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 761
Eco53kI GAGCTC 1 cut(s) 523
Eco57I CTGAAG 2 cut(s) 159, 345
EcoICRI GAGCTC 1 cut(s) 523
EcoO109I RGGNCCY 1 cut(s) 761
EcoRI GAATTC 2 cut(s) 181, 425
EcoRII CCWGG 1 cut(s) 436
EcoRV GATATC 1 cut(s) 237
EcoT14I CCWWGG 1 cut(s) 956
EcoT22I ATGCAT 1 cut(s) 533
EcoT38I GRGCYC 1 cut(s) 525
ErhI CCWWGG 1 cut(s) 956
FaeI CATG 4 cut(s) 251, 544, 911, 960
FatI CATG 4 cut(s) 247, 540, 907, 956
FauI CCCGC 1 cut(s) 77
FauNDI CATATG 1 cut(s) 895
Fnu4HI GCNGC 3 cut(s) 160, 336, 584
FokI GGATG 2 cut(s) 455, 881
FriOI GRGCYC 1 cut(s) 525
Fsp4HI GCNGC 3 cut(s) 160, 336, 584
FspBI CTAG 2 cut(s) 117, 638
GluI GCNGC 3 cut(s) 160, 336, 584
GsaI CCCAGC 1 cut(s) 893
HaeIII GGCC 2 cut(s) 256, 624
HapII CCGG 3 cut(s) 16, 47, 146
Hin1II CATG 4 cut(s) 251, 544, 911, 960
HinfI GANTC 5 cut(s) 244, 449, 772, 845, 917
HpaII CCGG 3 cut(s) 16, 47, 146
HphI GGTGA 3 cut(s) 160, 231, 716
Hpy188I TCNGA 3 cut(s) 28, 372, 769
Hpy188III TCNNGA 4 cut(s) 186, 248, 509, 670
HpyAV CCTTC 1 cut(s) 774
HpyCH4III ACNGT 2 cut(s) 403, 421
HpyCH4IV ACGT 2 cut(s) 607, 962
HpyCH4V TGCA 9 cut(s) 515, 531, 544, 583, 596, 613, 828, 872, 881
HpyF10VI GCNNNNNNNGC 3 cut(s) 81, 168, 878
HpySE526I ACGT 2 cut(s) 607, 962
Hsp92II CATG 4 cut(s) 251, 544, 911, 960
Kzo9I GATC 4 cut(s) 10, 290, 470, 706
LguI GCTCTTC 1 cut(s) 178
LmnI GCTCC 2 cut(s) 148, 528
Lsp1109I GCAGC 2 cut(s) 347, 595
LweI GCATC 2 cut(s) 502, 859
MaeI CTAG 2 cut(s) 117, 638
MaeII ACGT 2 cut(s) 607, 962
MaeIII GTNAC 3 cut(s) 136, 785, 918
MalI GATC 4 cut(s) 12, 292, 472, 708
MboI GATC 4 cut(s) 10, 290, 470, 706
MboII GAAGA 2 cut(s) 165, 926
MfeI CAATTG 1 cut(s) 597
MflI RGATCY 1 cut(s) 470
MhlI GDGCHC 2 cut(s) 308, 525
MlsI TGGCCA 1 cut(s) 624
MluCI AATT 9 cut(s) 54, 66, 181, 393, 425, 597, 652, 801, 873
MluI ACGCGT 1 cut(s) 569
MluNI TGGCCA 1 cut(s) 624
MlyI GAGTC 2 cut(s) 238, 926
MmeI TCCRAC 4 cut(s) 81, 264, 448, 544
MnlI CCTC 6 cut(s) 106, 119, 125, 325, 516, 942
Mox20I TGGCCA 1 cut(s) 624
Mph1103I ATGCAT 1 cut(s) 533
MscI TGGCCA 1 cut(s) 624
MseI TTAA 6 cut(s) 201, 377, 386, 657, 735, 861
MslI CAYNNNNRTG 1 cut(s) 906
Msp20I TGGCCA 1 cut(s) 624
MspI CCGG 3 cut(s) 16, 47, 146
MspR9I CCNGG 2 cut(s) 48, 438
MunI CAATTG 1 cut(s) 597
MvaI CCWGG 1 cut(s) 438
MvnI CGCG 1 cut(s) 571
MwoI GCNNNNNNNGC 3 cut(s) 81, 168, 878
NciI CCSGG 1 cut(s) 48
NcoI CCATGG 1 cut(s) 956
NdeI CATATG 1 cut(s) 895
NdeII GATC 4 cut(s) 10, 290, 470, 706
NlaIII CATG 4 cut(s) 251, 544, 911, 960
NlaIV GGNNCC 1 cut(s) 472
NmuCI GTSAC 1 cut(s) 918
NsiI ATGCAT 1 cut(s) 533
NspV TTCGAA 1 cut(s) 209
PagI TCATGA 1 cut(s) 247
PciSI GCTCTTC 1 cut(s) 178
PfeI GAWTC 3 cut(s) 449, 772, 845
PflMI CCANNNNNTGG 1 cut(s) 896
PkrI GCNGC 3 cut(s) 161, 337, 585
PleI GAGTC 2 cut(s) 238, 925
PpsI GAGTC 2 cut(s) 238, 925
PpuMI RGGWCCY 1 cut(s) 761
Psp124BI GAGCTC 1 cut(s) 525
Psp1406I AACGTT 1 cut(s) 607
Psp5II RGGWCCY 1 cut(s) 761
Psp6I CCWGG 1 cut(s) 436
PspFI CCCAGC 1 cut(s) 889
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 1 cut(s) 472
PspPI GGNCC 1 cut(s) 761
PspPPI RGGWCCY 1 cut(s) 761
PsuI RGATCY 1 cut(s) 470
RsaI GTAC 1 cut(s) 487
RsaNI GTAC 1 cut(s) 486
RseI CAYNNNNRTG 1 cut(s) 906
SacI GAGCTC 1 cut(s) 525
SapI GCTCTTC 1 cut(s) 178
SaqAI TTAA 6 cut(s) 201, 377, 386, 657, 735, 861
SatI GCNGC 3 cut(s) 160, 336, 584
Sau3AI GATC 4 cut(s) 10, 290, 470, 706
Sau96I GGNCC 1 cut(s) 761
SchI GAGTC 2 cut(s) 238, 926
ScrFI CCNGG 2 cut(s) 48, 438
SduI GDGCHC 2 cut(s) 308, 525
SfaNI GCATC 2 cut(s) 502, 859
SfcI CTRYAG 2 cut(s) 36, 420
SfuI TTCGAA 1 cut(s) 209
SinI GGWCC 1 cut(s) 761
SmiMI CAYNNNNRTG 1 cut(s) 906
Sse9I AATT 9 cut(s) 54, 66, 181, 393, 425, 597, 652, 801, 873
SspMI CTAG 2 cut(s) 117, 638
SstI GAGCTC 1 cut(s) 525
StyD4I CCNGG 2 cut(s) 46, 436
StyI CCWWGG 1 cut(s) 956
TaaI ACNGT 2 cut(s) 403, 421
TaiI ACGT 2 cut(s) 610, 965
TaqI TCGA 2 cut(s) 209, 429
TasI AATT 9 cut(s) 54, 66, 181, 393, 425, 597, 652, 801, 873
TauI GCSGC 1 cut(s) 162
TfiI GAWTC 3 cut(s) 449, 772, 845
Tru1I TTAA 6 cut(s) 201, 377, 386, 657, 735, 861
Tru9I TTAA 6 cut(s) 201, 377, 386, 657, 735, 861
TscAI CASTG 3 cut(s) 234, 481, 927
TseFI GTSAC 1 cut(s) 918
TseI GCWGC 2 cut(s) 335, 583
Tsp45I GTSAC 1 cut(s) 918
TspDTI ATGAA 2 cut(s) 264, 926
TspGWI ACGGA 3 cut(s) 123, 314, 429
TspRI CASTG 3 cut(s) 234, 481, 927
Van91I CCANNNNNTGG 1 cut(s) 896
VpaK11BI GGWCC 1 cut(s) 761
XapI RAATTY 3 cut(s) 181, 425, 652
XspI CTAG 2 cut(s) 117, 638
Zsp2I ATGCAT 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.